Analysis of regulator of g protein signaling (RGS) function in growth, development and pathogenicity of magnaporthe grisea 2

Figure 16 WT TMT1398 Figure 17 A B MDDTSRPDQVFKITSTQGIDHNNKPTAPSEVVNEKSHAALSSAHLHSTLPAPLPDTTAKPGSRKSLL LQQHQQQKSTTESTNAAPDSKPQRAGGIFAYAAAALDRTLSESRNIRSRPSLNRLSVSVLPESTTTT SSPRQPTGGNNGSSSPEKKARNRASVSNLRLSSLGTPPSQPYNDTDPSQPAPVHVQRLSNKMHQTS SRLLRMTDDDRPFTKDFKDLFATLIVSMLPLTPHRVRLTKIDHTFLSEDAINNLGSLKFSQSNRMPD PKDVARIVTTTTTTTFSMAKEMARSMLQRFLEARLIESADGKYQQVYTMRGTVWQLTPKGISILD SFASRNGIQDRQIPEVVGSSLAQLVTLERDPATDKLVKDFHTIEVIFGRFIGIHGPNVKSSVSSADSD SLSDYRDGLTGVKMAPERKVNGKIHKDTFTGKAASEWLMDCCTTVDRREAVEIASLFVEYELIEA LQQDRSHIQQFPGCQVFQPTKHAIYHMTSKGKDMINGSVPRGRASEGDATHSATHRHGVARDSNT QRMDKILNDAALRLLFRENLRETHCEENLSFYLDVEEFVRLCRSAVRQAQLAQSSPANSAEAKKQ ALDGVKEVMAQAYGIYNAFLAPGSPCELNIDHQLRNNLATRMTKAVGQDSAMVETLQEVMALF EDAQNAVFKLMASDSVPKFLRSRRYEHLLKNYDFDSVSLESSPRVPERSQSRSQRL C Figure 18 Rgs1 Cprgs-1 FlbA Sst2 1 1 --MDDTSRPDQVFKITSTQGIDHNNKPTAPSEVVNEKSHAALSSAHLHSTLPAPLPDTTA -----------------------------------------------------------MPTISSTPLDQGSPPSSQHHHPSCHSHTSTTSVPVSSTPADIPSSSIISPLTSPAPTTSS ----------MVDKNRTLHELSSKNFSRTPNGLIFTNDLKTVYSIFLICLDLKEKKHSSD Rgs1 Cprgs-1 FlbA Sst2 59 61 51 KPGSRKSLLLQQHQQQKSTTESTN--------AAPDSKPQRAGGIFAYAAAALDRTLSES -----------------------------------------------------------TTTTANTTATTTTSFTGSVIGSISRRNRRSFAALAREKTSNALANLSAIGSTTSSSLRTS TKSFLLTAFTKHFHFTFTYQEAIKAMG-----QLELKVDMNTTCINVSYNIKPSLARHLL Rgs1 Cprgs-1 FlbA Sst2 111 121 106 RNIRSRPSLNRLSVSVLPESTTTTSSPRQPTGGNNGSSSPEKKARNRASVSNLRLSSLG-----------------------------------------------------------TSSGSLSKHSRKPSQISTHEIAGVSPLTPPLSDESTSSNQSSPACIETSEVESQQSLSGR TLFMSSKLLHTPQDRTRGEPKEKVLFQPTPKGVAVLQKYVRDIGLKTMPDILLSSFNSMK Rgs1 Cprgs-1 FlbA Sst2 170 181 166 TPPSQPYNDTDPSQPAPVHVQRLSNKMHQTSSRLLRMTDDDRPFTKDFKDLFATLIVSML --------------------------MHQTSSRLLRMTDDDRPFTKDFKDLFATLIVSLL RRHTLQRNSPISEQQLEAGFAALPSKMHQTSSRLLRMTEDDRPFTKDFMDLFSTLMVSLK LFTFERSSVTDSIIHSDYLIHILFIKMMGAKPNVWSPTNADDPLPCLSSLLEYTNNDDTF Rgs1 Cprgs-1 FlbA Sst2 230 35 241 226 PLTPHRVRLTKIDHTFLSEDAINNLGSLKFSQSNRMPDPKDVARIVTTTTTTTFSMAKEM PLSAHRVRLTKVEHTFLSEDAINNLGSLKFSQSNRMPDPKDPSRIVTTTTTTTFSMAKDM -LDSHRVRFTKYDHTFTSEEAINNLGSLKFSQSNRMPDPKDPSRIVTTTTTTTFSMAKEM TFEKSKPEQGWQAQIGNIDINDLERVSPLAHRFFTNPDSESHTQYYVSNAGIRLFENKTF Rgs1 Cprgs-1 FlbA Sst2 290 95 300 286 ARSMLQRFLEARLIESADGKYQQVYTMRGTVWQLTPKGISILDSFASRNGIQDRQIPEVV ARSICQRFLEARLIESADGKLQQVYTMKGSVWQLTPKGIAILDQFCSKNGIQQKQVAELI ARSVCQRFVDARFIEPVDGKALPIFPLKGALFQLTPKGINILQRFCQRNGITARHVIDVL GTSKKIVIKYTFTTKAIWQWIMDCTDIMHVKEAVSLAALFLKTGLIVPVLLQPSRTDKKK Rgs1 Cprgs-1 FlbA Sst2 350 155 360 346 G--SSLAQLVTLERDPATDKLVKDFHTIEVIFGRFIGIHGPNVKSSVSSADSDSLSDYRD G--PALTQLVSLERESSTDKLQTDRATMEVVFRRFTGLKGLNIKSNVNAADSESLSDYSD ESPRNTMQLVNLERDTETDKLSHDRATIEVIFRRFAGQDGPNVKSSISTSDSDSLSDYSN FQISRSSFFTLSKRGWDLVSWTGCKSNNIRAPNGSTIDLDFTLRGHMTVRDEKKTLDDSE Rgs1 Cprgs-1 FlbA Sst2 408 213 420 406 GLTGVKMAPERKVNGKIHKDTFTGK-AASEWLMDCCTTVDRREAVEIASLFVEYELIEAL GLTGVKMAAERKIGGKTYKETFTGK-AATDWLMDCSTTVDRRETVEIASYFVEFGLMECV GLVGVKMAKERKINDKIYMNTFTGK-AAVDWLMDCSTTIERRETVLIAELFVKYGLITML GFSQDMLISSSNLNKLDYVLTDPGMRYLFRRHLEKELCVENLDVFIEIKRFLKKMTILKK Rgs1 Cprgs-1 FlbA Sst2 467 272 479 466 QQDRSHIQQFPGCQVFQPTKHAIYHMTSKGKDMINGSVPRGRASEGDATHSATHRHG-VA QQDRAYTAQYPGSQLFQPTKHSIYQITPNGKDLINGMNSRGRTSDAESTPRDHKPNEKLP QEDKGYPQPDASIVVFQPSKYAIYGITERGQRVCG-------WIARDKSRETFYDNRGMP LIDSKHCDKKSNTSTSKNNIVKTIDSALMKQANECLEMAYHIYSSYIMIGSPYQLNIHHN Rgs1 Cprgs-1 FlbA Sst2 526 332 532 526 RDSNTQRMDKILNDAALRLLFRENLR--ETHCEENLSFYLDVEEFVRLCRSAVRQAQLAQ RDSNTQRLEKILSDPALRLLFRENLR--ETHCEENLSFYLDVDEFVKQCKLAIRNAQKNP KDSNNARLNHILQDPALRLLFREFLR--FSLCEENLSFYLDVSEFTAAYHKSEKMGAFKK LRQNISDIMLHPHSPLSEHFPTNLYDPSPASAESAASSISSTEADTLGEPPEVSLKPSKN Rgs1 Cprgs-1 FlbA Sst2 584 390 590 586 SSPANSAEAKKQALDGVKEVMAQAYGIYNAFLAPGSPCELNIDHQLRNNLATRMTKAVGQ STTS---------MDGIKEIMAQAYGIYNAFLAPGSPCELNIDHQLRSNLATRMTKAVGQ -------------ADAVRETLAAAYGLYNAFLAPGSPCELNIDHALRNSLASRMTKAVGD LSNENCSFKKQGFKHQLKEYKPAPLTLAETHSPNASVENSHTIVRYGMDNTQNDTKSVES Rgs1 Cprgs-1 FlbA Sst2 644 441 637 646 DSAMVETLQEVMALFEDAQNAVFKLMASDS------VPKFLRSRRYEHLLKNYDFDSVSL DVAMIDTLHEVTALFEDAQNAVFKLMASDS------VPKFLRTSKYESVLKNYDFDSVSY DESMFKSLQEVVQLFEMAQTSVFKLMSSFTDEDQDSVPKFLRDPKYAVVLQEHDVDLIGA FPATLKVLRKLYPLFEIVSNEMYRLMNNDS------FQKFTQSDVYKDASALIEIQEKC- Rgs1 Cprgs-1 FlbA Sst2 698 ESSP-----RVPERSQSRSQRL 495 PVG------KGLDRSQSRSNRK 697 PRAYSPTPAPVPERSMSRSARS ---------------------- Figure 19 Figure 20 A B HPH1 RGS1 Figure 21 A rgs1D WT Rgs1 OP Complemented B WT rgs1D Complemented Rgs1 OP _ Figure 22 Inductive Non-inductive _ Figure 23 WT rgs1D Complemented Figure 24 Figure 25 Figure 26 Rgs1 MG00990 MG03146 MG03726 MG08735 MG09618 [...]...Figure 25 Figure 26 Rgs1 MG00990 MG03146 MG03 726 MG08735 MG09618 . OP Figure 21 _ A WT B Rgs1 OP rgs1D Comple- mented Figure 22 _ Inductive Non-inductive Complemented WT rgs1D Figure 23 Figure 24 Figure 25 Rgs1 MG00990 MG03146 Figure 26 MG09618 MG03 726 MG08735 . PLSAHRVRLTKVEHTFLSEDAINNLGSLKFSQSNRMPDPKDPSRIVTTTTTTTFSMAKDM FlbA 24 1 -LDSHRVRFTKYDHTFTSEEAINNLGSLKFSQSNRMPDPKDPSRIVTTTTTTTFSMAKEM Sst2 22 6 TFEKSKPEQGWQAQIGNIDINDLERVSPLAHRFFTNPDSESHTQYYVSNAGIRLFENKTF Rgs1 29 0 ARSMLQRFLEARLIESADGKYQQVYTMRGTVWQLTPKGISILDSFASRNGIQDRQIPEVV. FlbA 420 GLVGVKMAKERKINDKIYMNTFTGK-AAVDWLMDCSTTIERRETVLIAELFVKYGLITML Sst2 406 GFSQDMLISSSNLNKLDYVLTDPGMRYLFRRHLEKELCVENLDVFIEIKRFLKKMTILKK Rgs1 467 QQDRSHIQQFPGCQVFQPTKHAIYHMTSKGKDMINGSVPRGRASEGDATHSATHRHG-VA