Characterization of fetomaternal microchimerism in a murine model 2

Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 E12.5-­‐1 28.00 23.20 28.00 14.52 28.00 28.00 28.00 28.00 28.00 28.00 20.75 28.00 28.00 23.77 24.87 28.00 22.20 28.00 28.00 22.87 21.09 28.00 23.74 28.00 E12.5-­‐11 28.00 25.82 28.00 14.66 28.00 28.00 28.00 28.00 25.80 28.00 19.21 28.00 28.00 28.00 28.00 21.88 24.25 28.00 28.00 28.00 20.86 22.72 28.00 28.00 E12.5-­‐3.6 10.14 14.99 28.00 15.28 18.59 28.00 20.21 28.00 28.00 28.00 23.55 28.00 28.00 21.87 28.00 28.00 24.13 28.00 28.00 20.60 22.63 28.00 28.00 28.00 E12.5-­‐3.7 28.00 21.96 28.00 15.08 28.00 28.00 28.00 28.00 28.00 28.00 22.41 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 22.53 28.00 22.42 28.00 Raw  Ct  Value E12.5-­‐2 E12.5-­‐2.4 28.00 28.00 14.37 23.28 28.00 28.00 14.45 14.93 16.54 28.00 28.00 28.00 16.54 28.00 28.00 28.00 28.00 28.00 28.00 28.00 21.51 20.91 28.00 28.00 23.34 28.00 30.34 22.19 26.56 28.00 28.00 28.00 20.37 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 21.32 28.00 24.66 28.00 28.00 28.00 28.00 Raw  Ct  Value E12.5-­‐3.8 E12.5-­‐7 28.00 28.00 19.45 21.01 28.00 28.00 16.43 16.42 20.02 21.02 28.00 28.00 20.23 28.00 28.00 28.00 28.00 28.00 28.00 28.00 22.07 19.97 28.00 28.00 28.00 28.00 22.93 28.00 25.84 28.00 28.00 28.00 21.95 25.40 28.00 28.00 28.00 28.00 22.79 28.00 23.69 25.28 23.16 28.00 28.00 28.00 28.00 28.00 E12.5-­‐2.8 21.10 21.74 28.00 14.62 28.00 28.00 28.00 28.00 28.00 28.00 21.73 28.00 28.00 28.00 24.36 28.00 22.56 28.00 28.00 28.00 23.05 513.57 28.00 28.00 E12.5-­‐3.1 28.00 24.58 28.00 16.06 28.00 28.00 28.00 28.00 28.00 28.00 23.13 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 513.24 25.43 22.87 28.00 E12.5-­‐8 28.00 18.30 28.00 17.29 22.02 28.00 20.06 28.00 28.00 28.00 24.04 28.00 28.00 24.69 22.00 28.00 28.00 28.00 28.00 28.00 28.96 28.00 25.12 28.00 E12.5-­‐5-­‐1.4 28.00 22.51 28.00 16.75 28.00 28.00 28.00 28.00 28.00 28.00 24.82 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 25.07 28.00 28.00 28.00 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 Raw  Ct  Value E12.5-­‐10 E12.5-­‐5-­‐3.11 E12.5-­‐5-­‐3.14 E12.5-­‐5-­‐3.2 E12.5-­‐5-­‐3.3 E12.5-­‐5-­‐3.4 28.00 28.00 27.01 28.00 28.00 28.00 24.17 17.32 15.29 20.34 20.64 17.91 28.00 28.00 28.00 28.00 28.00 28.00 15.99 16.94 16.32 16.59 16.77 15.87 28.00 22.41 28.00 22.05 20.94 20.18 28.00 28.00 28.00 28.00 28.00 28.00 28.00 20.95 21.54 19.93 28.00 21.63 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 22.94 24.27 26.53 22.70 23.08 22.97 28.00 28.00 28.00 28.00 28.00 28.00 28.00 22.14 28.00 28.00 28.00 28.00 28.00 22.92 26.19 28.00 28.00 28.00 28.00 24.82 30.01 19.00 28.00 20.20 28.00 28.00 28.00 28.00 28.00 28.00 24.03 21.10 21.13 24.96 24.49 24.04 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 22.69 28.00 28.00 28.00 21.07 24.48 23.78 25.95 24.44 28.32 23.30 28.00 28.41 28.48 26.76 28.00 23.43 28.00 28.00 28.00 24.03 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 Raw  Ct  Value E12.5-­‐5-­‐1.7 E12.5-­‐10-­‐1.4 E12.5-­‐10-­‐1.5E12.5-­‐10-­‐2.10E12.5-­‐10-­‐2.12E12.5-­‐10-­‐2.9 28.00 25.35 28.00 28.00 28.00 22.50 17.22 23.58 26.05 21.40 28.00 26.35 28.00 28.00 28.00 28.00 28.00 28.00 16.40 17.18 17.40 19.60 18.13 18.62 20.59 28.00 28.00 23.59 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 22.59 28.00 28.00 28.00 28.00 28.00 28.00 20.75 28.00 28.00 23.08 28.00 28.00 26.87 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 22.03 20.60 22.15 28.15 24.37 24.24 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 23.46 28.00 28.00 28.00 28.00 21.30 23.97 28.00 28.00 28.26 24.49 9.53 9.46 28.00 15.64 22.59 28.00 28.00 28.00 28.00 28.00 28.00 22.35 20.17 20.75 21.45 21.31 21.74 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 24.97 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 Gene Raw  Ct  Value E12.5-­‐5-­‐3.7 E12.5-­‐5-­‐3.9 E12.5-­‐10-­‐1.3 E12.5-­‐10-­‐5.1E12.5-­‐10-­‐5.14E12.5-­‐10-­‐5.16 Oct-­‐04 28.00 28.00 28.00 28.00 28.00 28.00 ACTIN 18.78 23.01 20.06 20.76 24.79 19.78 BMP4 28.00 28.00 28.00 28.00 28.00 28.00 T 16.24 17.49 19.05 19.52 18.90 18.88 CD31 19.80 28.00 23.81 28.00 28.00 22.90 CDX2 28.00 28.00 28.00 28.00 28.00 28.00 CXCR4 21.26 28.00 21.00 28.00 28.00 20.14 EOMES 28.00 28.00 28.00 28.00 28.00 28.00 FOXA2 28.00 28.00 28.00 28.00 28.00 28.00 GATA4 28.00 28.00 28.00 28.00 28.00 28.00 GFP4 23.29 25.14 28.00 19.09 24.35 28.00 HNF4A 28.00 28.00 28.00 28.00 28.00 28.00 MIX1 28.00 28.00 28.00 28.00 28.00 28.00 NANOG 28.00 28.00 28.00 28.00 28.00 27.81 NESTIN 23.29 28.00 22.79 28.00 28.00 23.14 NKX2.5 28.00 28.00 28.00 28.00 28.00 28.00 OTX2 28.01 22.07 20.19 21.39 21.71 20.74 PAX6 28.00 28.00 28.00 28.00 28.00 28.00 PDX1 28.00 28.00 28.00 28.00 28.00 28.00 RUNX 28.00 29.14 28.00 28.00 28.00 23.22 SOX2 28.00 28.00 28.00 28.00 28.00 28.00 SOX7 27.80 28.00 28.00 28.00 28.00 28.00 TBX6 28.00 28.00 28.00 28.00 28.00 28.00 ZIC1 28.00 28.00 28.00 28.00 28.00 28.00 Gene Raw  Ct  Value E12.5-­‐10-­‐3.10E12.5-­‐10-­‐3.13E12.5-­‐10-­‐3.6E12.5-­‐10-­‐5.28E12.5-­‐10-­‐5.31 Oct-­‐04 28.00 31.39 28.00 28.00 23.06 ACTIN 18.39 16.63 22.83 25.76 24.30 BMP4 28.00 28.00 28.00 28.00 28.00 T 19.60 18.90 17.72 18.16 18.61 CD31 28.00 23.18 28.00 28.00 28.00 CDX2 28.00 28.00 28.00 28.00 28.00 CXCR4 20.13 23.86 28.00 28.00 28.00 EOMES 28.00 28.00 23.60 28.00 28.00 FOXA2 28.00 28.00 28.00 23.47 28.00 GATA4 28.00 28.00 28.00 28.00 28.00 GFP4 25.60 28.00 21.38 22.48 22.12 HNF4A 28.00 28.00 28.00 28.00 28.00 MIX1 28.00 30.76 28.00 23.87 28.00 NANOG 28.00 28.00 22.55 26.06 28.00 NESTIN 28.00 28.00 17.84 25.06 14.34 NKX2.5 28.00 28.00 28.00 28.00 28.00 OTX2 22.06 22.32 20.29 19.65 19.02 PAX6 28.00 28.00 28.00 28.00 28.00 PDX1 28.00 28.00 28.00 28.00 28.00 RUNX 28.00 24.76 28.00 28.00 28.00 SOX2 23.97 28.00 28.00 28.00 28.00 SOX7 28.00 28.00 28.00 28.00 28.00 TBX6 28.00 28.00 28.00 25.58 28.00 ZIC1 28.00 28.00 28.00 28.00 28.00 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 E16.5-­‐1 E16.5-­‐11 26.01 28.00 18.53 16.68 28.00 28.00 14.92 14.40 20.95 20.23 28.00 28.00 20.51 18.55 28.00 28.00 28.00 28.00 28.00 28.00 19.46 22.54 28.00 28.00 28.00 28.00 28.00 30.20 28.00 28.00 28.00 25.44 22.39 25.18 28.00 28.00 28.00 28.00 28.00 28.00 20.96 21.33 24.50 28.00 28.00 28.00 28.00 28.00 E16.5-­‐2.7 E16.5-­‐2.8 28.00 22.07 19.71 19.53 28.00 28.00 15.64 15.35 20.97 20.30 28.00 28.00 18.59 19.14 28.00 28.00 28.00 28.00 28.00 28.00 23.32 21.11 28.00 28.00 28.00 28.00 515.52 27.93 26.30 25.59 28.00 28.00 28.00 21.53 28.00 28.00 28.00 28.00 26.69 22.21 22.03 23.58 23.11 23.24 28.00 28.00 28.00 28.00 Raw  Ct  Value E16.5-­‐2.11 E16.5-­‐2.12 E16.5-­‐2.2 E16.5-­‐2.4 24.09 21.89 28.00 28.00 17.37 19.51 19.49 18.42 28.00 28.00 28.00 28.00 16.71 15.87 17.32 14.95 28.00 20.11 22.85 19.80 28.00 28.00 28.00 32.42 17.43 18.06 20.77 19.59 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 23.65 23.60 23.38 21.37 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 23.70 21.37 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 24.51 28.00 22.79 28.00 24.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 Raw  Ct  Value E16.5-­‐3 E16.5-­‐3.1 E16.5-­‐3.3 E16.5-­‐3.4 28.00 28.00 21.92 28.00 25.19 18.45 19.03 20.64 28.00 28.00 28.00 28.00 14.67 16.90 16.65 17.25 21.52 20.52 20.74 22.88 28.00 28.00 28.00 28.00 28.00 20.31 18.96 20.07 28.00 28.00 28.00 28.00 23.35 21.90 23.30 28.00 28.00 28.00 28.00 28.00 17.94 22.59 22.49 22.74 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 27.09 22.01 22.83 28.00 28.00 28.00 28.00 21.67 28.00 28.00 28.00 510.84 22.22 28.00 24.49 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 22.49 28.00 28.00 21.95 28.00 25.84 29.05 23.28 22.82 28.00 26.92 28.00 22.78 28.00 28.00 28.00 28.00 28.00 28.00 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 E16.5-­‐3.5 E16.5-­‐3.7 22.94 28.00 18.76 15.94 28.00 28.00 16.25 16.40 22.02 20.27 28.00 28.00 21.09 18.51 28.00 28.00 28.00 28.00 28.00 28.00 23.67 21.72 28.00 28.00 24.94 27.73 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 22.04 22.87 28.00 28.00 28.00 28.00 28.00 28.00 28.00 E16.5-­‐4-­‐2.9 E16.5-­‐4-­‐3.1 22.90 28.00 26.52 22.96 28.00 28.00 28.00 16.86 15.77 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 22.56 22.96 28.00 28.00 24.98 28.00 25.05 22.96 26.26 21.54 28.00 28.00 23.81 24.05 28.00 28.00 28.51 28.00 28.00 28.00 22.65 21.94 23.47 24.72 28.00 28.00 28.00 28.00 Raw  Ct  Value E16.5-­‐3.8 E16.5-­‐7 E16.5-­‐4-­‐1.3 E16.5-­‐4-­‐2.5 28.00 28.00 28.00 28.00 18.53 15.22 18.99 23.52 28.00 28.00 28.00 28.00 15.60 14.28 16.99 16.43 21.19 18.73 20.24 28.00 28.00 28.00 28.00 28.00 17.75 16.82 21.50 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 23.32 20.17 23.96 17.02 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 24.16 28.00 22.50 28.00 28.00 19.04 28.00 17.73 28.00 28.00 28.00 28.00 22.42 23.36 25.50 26.46 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 27.68 28.00 22.06 23.29 23.70 24.55 28.00 28.24 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 Raw  Ct  Value E16.5-­‐4-­‐3.11 E16.5-­‐4-­‐4.4 E16.5-­‐6-­‐1.2 E16.5-­‐6-­‐1.4 28.00 28.00 17.04 27.73 22.90 21.85 18.53 18.88 28.00 28.00 28.00 28.00 16.72 15.86 17.38 17.61 28.00 28.00 28.00 21.07 28.00 28.00 28.00 28.00 28.00 28.00 17.70 19.42 28.00 28.00 28.00 28.00 23.09 27.03 28.00 28.00 28.00 28.00 28.00 28.00 19.88 22.35 20.57 28.00 28.00 28.00 28.00 28.00 28.00 23.93 28.00 26.36 28.00 28.00 28.00 25.25 20.36 21.31 28.00 28.00 28.00 28.00 28.00 28.00 22.83 22.02 23.58 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 22.33 28.00 24.54 22.78 28.00 24.47 26.78 26.57 28.00 27.55 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 E16.5-­‐4-­‐2.8 28.00 23.49 28.00 16.25 28.00 28.00 28.00 28.00 26.49 28.00 22.85 28.00 28.00 28.00 26.89 26.75 23.16 28.00 28.00 28.00 28.02 22.53 28.00 28.00 E16.5-­‐6-­‐1.7 21.95 19.48 28.00 15.82 21.36 28.00 18.96 28.00 28.00 28.00 22.08 28.00 28.00 22.39 28.00 21.72 23.49 32.93 28.00 20.78 25.76 24.22 28.00 28.00 Raw  Ct  Value E16.5-­‐10-­‐2.7 E16.5-­‐6-­‐2.1 E16.5-­‐6-­‐2.12 E16.5-­‐6-­‐2.6 E16.5-­‐6-­‐2.7 28.00 28.00 26.12 20.61 28.00 18.42 19.83 17.78 17.30 20.36 28.00 28.00 28.00 28.00 28.00 17.75 19.78 18.27 18.13 18.08 22.22 24.18 20.10 19.93 21.87 28.00 28.00 28.00 28.00 28.00 22.88 23.90 21.34 20.67 21.15 22.90 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 24.91 24.11 24.59 22.98 26.65 28.00 28.00 28.00 28.00 28.00 28.00 28.00 25.65 28.00 28.00 23.13 26.63 25.56 24.62 25.54 11.91 28.00 30.73 28.00 28.00 28.00 28.00 28.00 28.00 28.00 21.97 28.00 22.56 28.00 26.56 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 24.57 28.00 28.00 22.24 23.41 28.00 22.67 28.00 28.00 24.33 29.08 28.30 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 Raw  Ct  Value E16.5-­‐6-­‐3.2 E16.5-­‐6-­‐3.4 E16.5-­‐6-­‐3.7 E16.5-­‐6-­‐3.8 E16.5-­‐6-­‐3.9 28.00 28.00 28.00 28.00 28.00 18.99 18.75 18.43 18.13 23.55 28.00 28.00 28.00 28.00 28.00 18.61 18.79 16.86 17.17 16.30 21.44 22.79 22.91 19.80 28.00 28.00 28.00 28.00 28.00 28.00 20.03 19.85 20.90 19.44 28.00 28.00 28.00 28.00 28.00 28.00 26.60 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 25.03 23.90 23.03 24.09 22.62 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 25.34 23.91 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 24.54 22.88 28.00 22.28 24.38 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 23.36 28.00 28.00 21.43 28.00 23.75 28.00 28.00 26.89 25.53 28.22 28.00 29.21 28.00 23.85 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 Raw  Ct  Value E16.5-­‐6-­‐3.12 E16.5-­‐6-­‐3.13 E16.5-­‐7-­‐1.10 E16.5-­‐7-­‐1.5 E16.5-­‐7-­‐2.10 E16.5-­‐7-­‐2.6 28.00 28.00 28.00 28.00 28.00 28.00 16.58 18.97 19.62 22.59 22.29 28.15 28.00 28.00 28.00 28.00 28.00 28.00 16.61 19.09 16.78 17.94 17.82 17.22 28.00 28.00 21.25 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 20.03 28.00 20.15 23.17 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 24.46 23.21 23.13 28.00 23.96 23.10 28.00 28.00 28.00 28.00 28.00 28.00 28.00 25.48 28.00 25.23 28.00 23.32 26.11 28.00 28.00 28.00 28.00 28.00 28.00 28.00 10.17 16.00 15.73 14.95 22.34 28.00 31.98 28.00 28.00 28.00 26.27 23.67 23.03 28.00 23.84 24.54 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 21.66 28.00 28.00 28.00 28.00 25.38 28.08 28.00 23.32 28.00 28.00 28.00 28.00 27.61 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 Raw  Ct  Value E16.5-­‐6-­‐4.2 E16.5-­‐7-­‐4.8 E16.5-­‐7-­‐4.9 E16.5-­‐8-­‐1.16 E16.5-­‐8-­‐1.18 E16.5-­‐8-­‐1.24 28.00 28.00 28.00 24.50 28.00 28.00 22.95 20.51 19.63 26.49 26.69 23.84 28.00 28.00 28.00 28.00 28.00 28.00 17.21 16.96 17.23 16.29 17.12 16.45 28.00 28.00 23.42 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 21.52 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 25.13 30.75 29.18 25.06 28.00 28.00 28.00 28.00 28.00 28.00 28.00 21.89 24.11 22.97 17.93 25.46 22.94 28.00 28.00 28.00 28.00 28.00 28.00 28.00 26.72 28.26 28.00 28.00 25.68 28.00 28.00 30.46 28.00 28.00 28.00 28.00 14.49 13.83 28.00 22.74 28.00 28.00 23.65 28.00 28.00 28.00 28.00 27.54 22.65 28.90 22.05 23.86 23.52 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 29.12 28.00 28.00 28.00 28.00 28.00 28.00 28.00 25.73 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 24.52 28.00 28.00 28.00 28.00 28.00 28.00 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 Raw  Ct  Value E16.5-­‐7-­‐2.8 E16.5-­‐7-­‐3.6 E16.5-­‐7-­‐4.14 E16.5-­‐7-­‐4.7 E16.5-­‐8-­‐2.16 E16.5-­‐8-­‐2.18 28.00 28.00 28.00 22.86 24.50 28.00 19.38 13.66 20.46 17.48 21.36 21.31 28.00 28.00 28.00 28.00 28.00 28.00 17.07 17.63 17.85 17.10 17.02 17.86 20.41 18.83 28.00 27.20 21.82 22.14 28.00 28.00 28.00 28.00 28.00 28.00 20.26 28.00 28.00 22.61 20.92 21.20 28.00 28.00 28.00 28.00 28.00 28.00 32.55 28.00 28.00 28.00 26.86 28.00 28.00 28.00 28.00 28.00 28.00 28.00 25.62 23.68 25.25 22.89 26.13 26.67 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 25.21 25.29 28.00 28.00 15.43 14.62 14.93 14.49 28.00 28.00 26.92 28.00 28.00 28.00 29.96 28.00 28.00 22.52 25.73 21.73 26.11 24.92 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 22.73 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 26.44 28.00 28.00 28.00 28.00 28.00 28.00 Raw  Ct  Value E16.5-­‐8-­‐2.11 E16.5-­‐8-­‐2.13 E16.5-­‐8-­‐2.14 E16.5-­‐9-­‐1.5 E16.5-­‐9-­‐1.8 E16.5-­‐9-­‐2.1 28.00 28.00 28.00 28.00 28.00 23.07 20.66 17.77 19.50 23.38 24.10 26.13 28.00 28.00 28.00 28.00 28.00 28.00 17.70 17.14 17.16 16.72 16.81 16.81 21.49 21.31 20.97 28.00 28.00 23.19 28.00 28.00 28.00 28.00 28.00 28.00 21.35 19.33 22.28 28.00 28.00 22.20 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.74 28.00 28.00 25.02 28.00 28.00 28.00 24.99 24.25 23.40 23.06 23.39 21.19 28.00 26.97 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 25.16 28.00 28.00 29.65 18.87 28.00 11.80 20.23 12.36 28.00 28.00 28.00 28.00 28.00 28.00 28.00 29.01 22.80 26.44 18.99 19.92 20.17 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 24.46 28.00 28.00 24.90 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 Raw  Ct  Value E16.5-­‐8-­‐2.24 E16.5-­‐8-­‐2.3 E16.5-­‐8-­‐2.4 E16.5-­‐8-­‐2.7 E16.5-­‐9-­‐1.1 E16.5-­‐9-­‐1.3 28.00 28.00 24.34 28.00 28.00 28.00 20.10 18.52 19.60 20.71 23.77 23.58 28.00 28.00 28.00 28.00 28.00 28.00 18.52 16.94 18.54 18.76 17.44 16.70 22.30 21.16 20.80 23.60 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 19.63 21.74 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 29.85 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 24.18 23.59 23.40 26.26 24.72 23.36 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 25.24 28.00 28.00 28.00 28.00 27.96 22.36 28.73 28.00 21.37 22.58 28.00 32.69 28.00 28.00 28.00 28.00 30.85 28.00 25.90 21.99 28.00 21.01 19.86 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 23.31 21.87 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 23.46 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 Raw  Ct  Value E16.5-­‐9-­‐2.15 E16.5-­‐9-­‐2.16 E16.5-­‐9-­‐2.17 E16.5-­‐9-­‐2.8 E16.5-­‐7-­‐4.10 28.00 28.00 27.53 28.00 22.16 19.00 18.97 19.91 23.78 16.77 28.00 28.00 28.00 28.00 28.00 17.42 16.77 16.81 17.03 16.57 20.66 21.53 21.58 28.00 19.58 28.00 28.00 28.00 28.00 28.00 20.05 21.81 20.36 28.00 18.17 28.00 28.00 28.00 28.00 28.00 28.00 30.71 29.53 28.00 28.00 28.00 28.00 28.00 28.00 28.00 22.41 19.76 21.03 19.00 21.51 28.00 28.00 28.00 28.00 28.00 28.00 25.39 28.00 26.68 28.00 28.00 29.11 28.00 28.00 23.75 17.83 22.44 28.00 22.43 13.17 27.46 27.29 27.78 28.00 31.64 19.00 19.40 18.79 19.02 26.04 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 20.96 28.00 28.00 28.00 28.00 28.00 22.78 23.38 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 Raw  Ct  Value E16.5-­‐9-­‐2.20 E16.5-­‐9-­‐2.2 28.00 28.00 20.83 20.35 28.00 28.00 17.37 17.82 28.00 22.96 28.00 28.00 20.04 20.21 28.00 28.00 28.00 28.00 28.00 28.00 25.76 26.60 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 19.78 19.84 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 P0-­‐2 28.00 18.77 28.00 15.90 19.63 28.00 20.36 28.00 24.87 28.00 22.25 28.00 28.00 28.00 21.76 28.00 20.34 28.00 28.00 24.88 28.00 28.00 28.00 28.00 P0-­‐2.3 22.39 21.02 28.00 15.88 28.00 28.00 28.00 28.00 28.00 28.00 22.75 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 23.56 23.86 28.00 28.00 P0-­‐4-­‐1.2 28.00 21.06 28.00 16.48 28.00 30.74 28.00 28.00 28.00 28.00 23.09 28.00 28.00 28.00 28.00 20.56 21.53 28.00 28.00 28.00 28.00 28.00 28.00 28.00 P0-­‐6-­‐1.12 20.72 23.16 28.00 15.48 28.00 28.00 28.00 28.00 28.00 28.00 21.63 28.00 28.00 28.00 19.13 28.00 25.08 28.00 28.00 28.00 24.18 28.00 24.29 28.00 Raw  Ct  Value P0-­‐2.6 P0-­‐4 28.00 28.00 23.48 18.61 28.00 28.00 15.35 16.46 28.00 20.90 28.00 28.00 28.00 20.99 28.00 28.00 28.00 28.00 28.00 28.00 16.71 23.56 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 22.54 28.00 28.00 28.00 28.00 28.00 28.00 22.09 21.89 28.00 23.50 28.00 28.00 28.00 28.00 28.00 Raw  Ct  Value P0-­‐6-­‐1.13 P0-­‐6-­‐1.14 28.00 28.00 22.83 28.00 28.00 16.12 15.28 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 22.64 21.48 28.00 28.00 28.00 28.00 23.68 24.00 29.94 28.00 28.00 28.00 24.47 21.73 28.00 28.00 28.00 28.00 28.00 28.00 25.00 25.00 26.22 27.30 28.00 28.00 28.00 28.00 P0-­‐5 28.00 19.51 28.00 15.13 20.91 28.00 19.46 28.00 28.00 28.00 23.29 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 21.27 74.41 28.00 28.00 28.00 P0-­‐8 28.00 23.13 28.00 14.79 28.00 28.00 28.00 28.00 28.00 28.00 22.82 28.00 28.00 28.00 21.77 28.00 21.55 28.00 28.00 23.31 21.60 24.68 28.00 28.00 P0-­‐6-­‐1.15 28.00 25.10 28.00 16.28 28.00 28.00 28.00 28.00 29.53 28.00 23.68 28.00 23.26 28.00 28.00 28.00 24.98 28.00 28.00 22.59 26.23 28.00 28.00 28.00 P0-­‐6-­‐1.16 22.20 999.00 28.00 15.66 27.52 28.00 26.89 28.00 22.97 28.00 23.38 28.00 27.07 26.76 22.55 28.00 23.46 28.00 28.00 27.14 22.10 27.54 28.00 28.00 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 P0-­‐6-­‐1.4 28.00 22.37 28.00 16.53 28.00 28.00 28.00 28.00 28.00 28.00 25.31 28.00 28.00 28.00 28.00 28.00 25.85 28.00 28.00 28.00 23.01 28.00 28.00 28.00 P0-­‐6-­‐1.5 28.00 25.00 28.00 16.42 28.00 28.00 28.00 28.00 28.00 28.00 22.98 28.00 28.00 28.00 28.00 28.00 24.18 28.00 28.00 28.00 24.66 28.00 28.00 28.00 P0-­‐6-­‐4.2 28.00 16.80 28.00 16.48 28.00 28.00 19.78 28.00 26.08 28.00 22.18 26.78 28.00 28.00 28.00 28.00 22.69 28.00 28.00 22.49 24.20 28.00 28.00 28.00 P0-­‐6-­‐4.5 28.00 28.00 28.00 15.28 28.00 28.00 28.00 28.00 28.00 28.00 23.94 28.00 28.00 27.66 28.00 28.00 21.44 28.00 28.00 28.00 24.89 25.33 28.18 28.00 Raw  Ct  Value P0-­‐6-­‐1.9 P0-­‐6-­‐2.11 28.00 28.00 25.49 25.56 28.00 28.00 16.90 14.87 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 24.24 25.56 28.00 28.00 28.00 28.00 28.00 22.67 28.00 28.00 28.00 28.00 27.92 23.56 28.00 28.00 28.00 28.00 28.00 28.00 22.32 22.44 27.57 28.00 28.00 28.00 28.00 28.00 Raw  Ct  Value P0-­‐9 P0-­‐8-­‐2.1 28.00 28.00 17.29 26.01 28.00 28.00 16.40 16.98 28.00 28.00 28.00 28.00 18.25 28.00 28.00 28.00 28.00 28.00 28.00 30.31 23.28 23.12 28.00 28.00 28.00 28.00 28.00 28.00 28.00 24.55 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 24.37 28.00 27.97 28.00 28.00 28.00 28.00 28.00 P0-­‐6-­‐2.15 28.00 23.86 28.00 16.28 28.00 28.00 28.00 28.00 28.00 28.00 23.65 28.00 28.00 23.65 28.00 28.00 28.00 28.00 28.00 28.00 21.97 28.00 28.00 28.00 P0-­‐6-­‐3.2 28.00 24.83 28.00 16.42 28.00 28.00 28.00 28.00 28.00 28.00 23.91 28.00 28.00 28.00 22.18 28.00 21.88 28.00 22.18 28.00 28.00 28.00 28.00 28.00 P0-­‐8-­‐2.19 28.00 28.00 28.00 17.21 28.00 28.00 28.00 28.00 28.42 28.00 29.96 28.00 28.00 28.00 28.00 28.00 24.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 P0-­‐8-­‐2.3 28.00 24.59 28.00 17.01 23.00 28.00 28.00 28.00 28.00 28.00 23.71 28.00 28.00 28.00 23.70 28.00 23.40 28.00 28.00 29.27 28.00 28.00 28.00 28.00 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 P0-­‐9-­‐2.1 23.47 21.32 28.00 18.45 28.00 28.00 28.00 28.00 28.00 28.00 21.72 28.00 28.00 28.00 20.30 28.00 22.29 28.00 28.00 28.00 28.00 28.00 28.00 28.00 P0-­‐9-­‐2.4 28.00 21.09 28.00 17.52 28.00 28.00 22.83 28.00 28.00 28.00 28.00 28.00 28.00 27.21 22.83 28.00 25.54 28.00 28.00 23.42 28.00 28.00 28.00 28.00 Raw  Ct  Value P0-­‐9-­‐5.17 P0-­‐9-­‐5.29 28.00 28.00 24.65 22.82 28.00 28.00 18.81 17.31 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 25.15 22.40 28.00 28.00 26.66 23.23 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 WT-­‐1 28.00 17.55 28.00 15.52 20.79 28.00 18.70 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 26.61 24.35 23.42 28.00 28.00 WT-­‐2 28.00 18.53 28.00 15.39 19.96 28.00 18.39 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 25.22 28.00 28.00 WT-­‐7 28.00 20.00 28.00 17.74 21.27 28.00 23.24 28.00 28.00 28.00 28.55 28.00 28.00 28.00 28.00 28.00 20.28 28.00 24.21 27.95 28.00 28.00 28.00 28.00 WT-­‐8 28.00 17.39 28.00 18.16 23.04 28.00 19.31 28.00 27.28 28.00 28.00 28.00 28.00 25.18 28.00 27.34 21.65 28.00 28.00 26.15 28.00 28.00 28.00 28.00 Raw  Ct  Value WT-­‐3 WT-­‐4 28.00 28.00 20.01 21.11 28.00 28.00 16.69 16.34 22.84 20.98 28.00 28.00 21.22 20.71 28.00 28.00 26.89 28.00 28.00 28.00 28.00 28.00 28.00 28.00 25.02 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 20.91 28.00 28.00 28.00 28.00 22.55 28.00 23.98 23.78 26.10 28.00 28.00 28.00 28.00 28.00 Raw  Ct  Value WT-­‐9 WT-­‐10 28.00 28.00 18.95 17.91 28.00 28.00 17.84 17.96 21.13 22.70 28.00 28.00 19.76 21.31 26.77 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 22.67 21.47 28.00 28.00 28.00 28.00 27.79 22.55 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 WT-­‐5 28.00 16.62 28.00 16.11 28.00 28.00 19.10 20.00 27.12 28.00 28.00 28.00 28.00 28.00 28.00 28.00 21.37 28.00 28.00 21.74 22.10 28.00 28.00 28.00 WT-­‐6 28.00 20.64 28.00 17.14 20.25 28.00 20.78 28.00 26.49 28.00 28.00 28.00 25.39 28.60 28.00 28.00 20.07 28.00 28.00 28.00 28.00 28.00 28.00 28.00 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 Gene Oct-­‐04 ACTIN BMP4 T CD31 CDX2 CXCR4 EOMES FOXA2 GATA4 GFP4 HNF4A MIX1 NANOG NESTIN NKX2.5 OTX2 PAX6 PDX1 RUNX SOX2 SOX7 TBX6 ZIC1 GFP-­‐1 28.00 21.24 28.00 15.24 21.87 28.00 21.42 28.00 28.00 28.00 21.10 28.00 28.00 28.00 26.06 28.00 28.00 28.00 28.00 28.00 28.00 28.00 23.14 28.00 GFP-­‐2 28.00 16.98 28.00 15.17 20.48 28.00 20.49 28.00 28.00 28.00 21.13 28.00 28.00 28.00 28.00 28.00 28.00 28.00 26.32 28.00 21.45 20.86 28.00 28.00 GFP-­‐7 28.00 18.66 28.00 16.78 28.00 28.00 19.29 28.00 28.00 28.00 20.55 28.00 28.33 28.00 28.00 28.00 24.56 28.00 28.00 28.00 24.08 28.00 28.00 28.00 GFP-­‐8 28.00 21.58 28.00 17.11 28.00 28.00 28.00 28.00 28.00 28.00 23.54 28.00 28.00 28.00 20.49 28.00 21.69 28.00 28.00 28.00 28.00 28.00 28.00 28.00 Raw  Ct  Value GFP-­‐3 GFP-­‐4 28.00 28.00 18.35 18.61 28.00 28.00 17.13 19.05 28.00 21.71 28.00 28.00 18.19 20.17 28.00 28.00 28.34 28.00 28.00 28.00 18.38 21.41 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 27.62 28.00 24.63 28.00 28.00 28.00 28.00 28.00 Raw  Ct  Value GFP-­‐9 GFP-­‐10 20.87 28.00 18.20 22.34 28.00 28.00 17.61 17.52 22.59 28.00 28.00 28.00 28.00 28.00 28.00 23.14 28.00 31.91 28.00 28.00 20.07 22.10 28.00 28.00 28.00 28.00 28.00 28.00 20.15 20.25 28.00 28.84 24.14 22.01 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 28.00 GFP-­‐5 28.00 16.93 28.00 16.82 28.00 28.00 18.34 28.00 28.00 28.00 18.79 28.00 28.00 26.09 24.98 28.00 25.21 28.00 28.00 21.52 26.70 23.68 28.00 28.00 GFP-­‐6 22.97 19.16 28.00 16.43 28.00 28.00 20.10 28.00 28.00 28.00 19.45 28.00 27.11 28.00 18.76 28.00 21.47 28.00 28.00 28.00 28.00 28.00 28.00 28.00 [...]... 28 .00 28 .00 28 .00 22 .67 28 .00 28 .00 28 .00 28 .00 27 . 92 23.56 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 22 . 32 22. 44 27 .57 28 .00 28 .00 28 .00 28 .00 28 .00 Raw  Ct  Value P0-­‐9 P0-­‐8-­ 2. 1 28 .00 28 .00 17 .29 26 .01 28 .00 28 .00 16.40 16.98 28 .00 28 .00 28 .00 28 .00 18 .25 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 30.31 23 .28 23 . 12 28.00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 24 .55 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00... 28 .00 27 .21 22 .83 28 .00 25 .54 28 .00 28 .00 23 . 42 28.00 28 .00 28 .00 28 .00 Raw  Ct  Value P0-­‐9-­‐5.17 P0-­‐9-­‐5 .29 28 .00 28 .00 24 .65 22 . 82 28.00 28 .00 18.81 17.31 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 25 .15 22 .40 28 .00 28 .00 26 .66 23 .23 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00... 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 21 .27 74.41 28 .00 28 .00 28 .00 P0-­‐8 28 .00 23 .13 28 .00 14.79 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 22 . 82 28.00 28 .00 28 .00 21 .77 28 .00 21 .55 28 .00 28 .00 23 .31 21 .60 24 .68 28 .00 28 .00 P0-­‐6-­‐1.15 28 .00 25 .10 28 .00 16 .28 28 .00 28 .00 28 .00 28 .00 29 .53 28 .00 23 .68 28 .00 23 .26 28 .00 28 .00 28 .00 24 .98 28 .00 28 .00 22 .59 26 .23 28 .00 28 .00 28 .00 P0-­‐6-­‐1.16 22 .20 999.00 28 .00... 28 .00 28 .00 28 .00 18.38 21 .41 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 27 . 62 28.00 24 .63 28 .00 28 .00 28 .00 28 .00 28 .00 Raw  Ct  Value GFP-­‐9 GFP-­‐10 20 .87 28 .00 18 .20 22 .34 28 .00 28 .00 17.61 17. 52 22. 59 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 23 .14 28 .00 31.91 28 .00 28 .00 20 .07 22 .10 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 20 .15 20 .25 28 .00 28 .84... 28 .00 28 .00 28 .00 24 .37 28 .00 27 .97 28 .00 28 .00 28 .00 28 .00 28 .00 P0-­‐6-­ 2. 15 28 .00 23 .86 28 .00 16 .28 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 23 .65 28 .00 28 .00 23 .65 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 21 .97 28 .00 28 .00 28 .00 P0-­‐6-­‐3 .2 28.00 24 .83 28 .00 16. 42 28.00 28 .00 28 .00 28 .00 28 .00 28 .00 23 .91 28 .00 28 .00 28 .00 22 .18 28 .00 21 .88 28 .00 22 .18 28 .00 28 .00 28 .00 28 .00 28 .00 P0-­‐8-­ 2. 19 28 .00 28 .00...  Value P0-­‐6-­‐1.13 P0-­‐6-­‐1.14 28 .00 28 .00 22 .83 28 .00 28 .00 16. 12 15 .28 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 22 .64 21 .48 28 .00 28 .00 28 .00 28 .00 23 .68 24 .00 29 .94 28 .00 28 .00 28 .00 24 .47 21 .73 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 25 .00 25 .00 26 .22 27 .30 28 .00 28 .00 28 .00 28 .00 P0-­‐5 28 .00 19.51 28 .00 15.13 20 .91 28 .00 19.46 28 .00 28 .00 28 .00 23 .29 28 .00 28 .00 28 .00... 28 .00 28 .00 WT-­ 2 28.00 18.53 28 .00 15.39 19.96 28 .00 18.39 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 25 .22 28 .00 28 .00 WT-­‐7 28 .00 20 .00 28 .00 17.74 21 .27 28 .00 23 .24 28 .00 28 .00 28 .00 28 .55 28 .00 28 .00 28 .00 28 .00 28 .00 20 .28 28 .00 24 .21 27 .95 28 .00 28 .00 28 .00 28 .00 WT-­‐8 28 .00 17.39 28 .00 18.16 23 .04 28 .00 19.31 28 .00 27 .28 28 .00 28 .00 28 .00 28 .00 25 .18 28 .00... 25 .18 28 .00 27 .34 21 .65 28 .00 28 .00 26 .15 28 .00 28 .00 28 .00 28 .00 Raw  Ct  Value WT-­‐3 WT-­‐4 28 .00 28 .00 20 .01 21 .11 28 .00 28 .00 16.69 16.34 22 .84 20 .98 28 .00 28 .00 21 .22 20 .71 28 .00 28 .00 26 .89 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 25 . 02 28.00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 20 .91 28 .00 28 .00 28 .00 28 .00 22 .55 28 .00 23 .98 23 .78 26 .10 28 .00 28 .00 28 .00 28 .00 28 .00 Raw  Ct  Value WT-­‐9... 28 .00 28 .00 P0-­ 2. 3 22 .39 21 . 02 28.00 15.88 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 22 .75 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 23 .56 23 .86 28 .00 28 .00 P0-­‐4-­‐1 .2 28.00 21 .06 28 .00 16.48 28 .00 30.74 28 .00 28 .00 28 .00 28 .00 23 .09 28 .00 28 .00 28 .00 28 .00 20 .56 21 .53 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 P0-­‐6-­‐1. 12 20. 72 23.16 28 .00 15.48 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 21 .63 28 .00... P0-­‐6-­‐1.4 28 .00 22 .37 28 .00 16.53 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 25 .31 28 .00 28 .00 28 .00 28 .00 28 .00 25 .85 28 .00 28 .00 28 .00 23 .01 28 .00 28 .00 28 .00 P0-­‐6-­‐1.5 28 .00 25 .00 28 .00 16. 42 28.00 28 .00 28 .00 28 .00 28 .00 28 .00 22 .98 28 .00 28 .00 28 .00 28 .00 28 .00 24 .18 28 .00 28 .00 28 .00 24 .66 28 .00 28 .00 28 .00 P0-­‐6-­‐4 .2 28.00 16.80 28 .00 16.48 28 .00 28 .00 19.78 28 .00 26 .08 28 .00 22 .18 26 .78 28 .00 28 .00 28 .00 . 28 .00 23 .49 24 .54 22 .88 28 .00 22 .28 24 .38 32. 93 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 20 .78 23 .36 28 .00 28 .00 21 .43 28 .00 25 .76 23 .75 28 .00 28 .00 26 .89 25 .53 24 .22 28 .22 . 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 27 .21 28 .00 28 .00 20 .30 22 .83 25 .15 22 .40 28 .00 28 .00 28 .00 28 .00 22 .29 25 .54 26 .66 23 .23 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 23 . 42. 19.33 22 .28 28 .00 28 .00 22 .20 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .00 28 .74 28 .00 28 .00 25 . 02 28.00 28 .00 28 .00 24 .99 24 .25 23 .40 23 .06 23 .39 21 .19 28 .00 26 .97 28 .00 28 .00