United States Patent 1191 Newell et a1. 1111“ 3,867,255 1451 Feb. 18,1975 1541 PROCESS OF MAKING YEAST PROTEIN ISOLATE HAVING REDUCED NUCLEIC ACID CONTENT [75] Inventors: Jon Albert Newell, Webster Groves; Robert Dudley Seeley, Crestwood; Ernest Aleck Robbins, High Ridge, all of Mo. [73] Assignee: Anheuser-Busch, Incorporated, St. Louis, Mo. [22] Filed: Nov. 29, 1972 [21] Appl. No.: 310,468 [52] US. Cl. 195/5, 260/112 R, 426/60, 426/148, 426/204, 426/212 [51] Int. Cl. A23j 1/18, A231 1/28 [58} Field of Search ,. 426/60, 148, 364, 20.4, 426/212, 229; 260/112 R; 195/5 [56] References Cited UNITED STATES PATENTS 3,268,412 8/1966 Champagnat ct a1 1. 195/3 3,585,179 6/1971 Samejima ct n1. 260/112 3,615,654 10/1971 Ayukawa ct a1. 99/9 3,634,194 1/1972 Frankenfeld ct a1 195/28 3,681,195 8/1972 Suekane et a1. . . . . . . . . . . . , . , . 195/4 3,718,541 2/1973 Kalina . . . . 1 . . . , . . . . . . . . . . . . . 195/28 3,725,075 4/1973 Muroi et a1, 99/14 Primary Examiner-lames R. Hoffman Attorney, Agent, or Firm-Gravely, Lieder & Woodruff [57] ' ABSTRACT This disclosure relates to a process of making a yeast protein isolate of low nucleic acid content. The pro cess involves adding exogenous nuclease to a solubles fraction separated from a mass of ruptured yeast cells to hydrolyze the nucleic acid. The preferred source of exogenous nuclease is malt sprouts. 17 Claims, 1 Drawing Figure . United States Patent 1191 Newell et a1. 1111“ 3,867,255 1451 Feb. 18,1975 1541 PROCESS OF. 426/60, 148, 364, 20.4, 426/212, 229; 260/112 R; 195/5 [56] References Cited UNITED STATES PATENTS 3,268,412 8/1966 Champagnat ct a1 1. 195/3 3,585,179 6/1971 Samejima. grams per day for a 70 ilogram adult male and The Pro tein Advisory Group of the United Nations System rec ommends that the amount of nucleic acid ingested per day from