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Luận văn : SỬ DỤNG KỸ THUẬT DAS-ELISA VÀ RT-PCR ĐỂ PHÁT HIỆN VIRUS GÂY BỆNH ĐỐM VÕNG TRÊN CÂY ĐU ĐỦ (Papaya ringspot virus) TẠI HAI TỈNH ĐỒNG NAI VÀ ĐỒNG THÁP part 10 pdf

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79 Phụ lục 12 Kết quả so với trình tự của CP-PRSV trên Genebank CLUSTAL X (1.83) multiple sequence alignment 3 AJ012649 TCCAAGAATGAAGCTGTGGATGCTGGTTTGGATGAAAAACTCAAAGAAAAAGAAAAACAG 60 3 AJ012649 AAAGAAAAAGAAAAACAAAAAGAAAAAGAAAAAGACAATGCTAGTGACGGAAATGATGTG 120 3 AJ012649 TCGACTAGCACAAGAACTGGAGAGAAAGATAGAGATGTCAATGTCGGGACCAGTGGAACT 180 3 1 CCGAGAATAAAATCATTTACTGACAAGATGATTTTACCGAAAATTAAAGGA 51 AJ012649 181 TTCACGGTTCCGAGAATTAAATCATTCACTGATAAGATGATTCTACCAAGAATTAAGGGA 240 190******** ******** ***** ********* **** * ****** *** 3 AAAACTGTCCTTAATTTTAAATCATCTTCTTCAGTATAATCCGCAACAAATTGATATCTC 111 AJ012649 AAGACTGTCCTTAATTT-AAATCATCTTCTTCAGTATAATCCGCAACAAATTGACATTTC 299 ** ************** ************************************ ** ** 3 AAACACTCGTGCCACTCAAACTCAATTCGAAAAGTGGTATGAGGGAGTGAGGAATGATTA 171 AJ012649 TAACACTCGTGCCACTCAGTCACAATTTGAGAAATGGTATGAGGGAGTGAGGAATGATTA 359 ***************** * ***** ** ** ************************** 3 CGGTCTCAACGATAACGAAATGCAAGTG-ATGTTAAACGGTTTGATGGTTTGGTGTATTG 230 AJ012649 TGGTCTGAATGATAATGAAATGCAAGTG-ATGCTGAATGGCTTGATGGTTTGGTGTATCG 418 ***** ** ***** ************ *** * ** ** ***************** * 3 AAAATGGTACATCTCCAGACTTATCTGGTGTTTGGGTAATGATGGATGGGGAAACACAAG 290 AJ012649 AGAATGGTACATCTCCAGACATATCTGGTGTTTGGGTTATGATGGATGGGGAAATTCAAG 478 * ****************** **************** **************** **** 3 TTGATTATCCCATTAAGCCTTTAATTGAACATGCAACTCCTTCGTTTAGGCAAATCATGG 350 AJ012649 TTGACTATCCAATCAAGCCTTTAATTGAGCATGCTACCCCGTCATTTAGGCAGATTATGG 538 **** ***** ** ************** ***** ** ** ** ******** ** **** 3 351 CTCACTTCAGTAACGCGGCAGAGGCATACATCGCAAAGAGGAATG 396 AJ012649 CTCACTTTAGTAACGCGGCAGAAGCATATATTGCAAAGAGAAATGCCACTGAGAGGTACA 598 ******* ************** ***** ** ******** **** 583 3 AJ012649 TGCCGCGGTATGGAATCAAGAGAAATTTGACTGACATTAGCCTCGCTAGATACGCTTTCG 3 AJ012649 ATTTCTATGAGGTTAATTCGAAAACACCTGATAGGGCTCGCGAAGCTCACATGCAGATGA 3 AJ012649 AAGCTGCAGCGCTGCGAAACACTAGTCGCAGAATGTTTGGTATGGACGGCAGTGTTAGTA 3 AJ012649 ATAAGGAAGAAAACACGGAGAGACACACAGTGGAAGATGTCAATAGAGACATGCACTCTC 3 AJ012649 TCCTGGGTATGCGCAACTGAATACTCGCGCTGGTGTGTTTGTCGAGCTCGACTCGACCTT 3 AJ012649 GTTTCACCTTATAGTACTAAATAAGCATTAGAATACAGTGTGGCTGTGCCACCACTTCTA 3 AJ012649 TTTTACAGTGAGGGTAGCCCTCCGTGCTTTTAGTATTATTCGAGTTCTCTGAGTCTCCAT 3 AJ012649 ACAGTGTGGGTGGCCCACGTGTTATTT ALIGN 396 NU 80 Phụ lục 13 Kết quả so với genome của PRSV trên Genebank CLUSTAL X (1.83) multiple sequence alignment 3 AY231130 1 AAATAAAACATCTCAACACAACATAATCAAAAGCATTTCAACAAACATCAACTTATCTCA 60 3 AY231130 TTTTAAATTGTTATAGTAAGCGACAATGTCTTCTTTGTATACTTTGCGAGCAGCAGCTCA 120 3 AY231130 ATACGATAGGAGGTTGGAGAGTAAGAAAGGTGCTGGCTGGATCGAGCACAAACTCGAGAG 180 3 AY231130 GAAAGGGGATAAAGGAAACACTCACTATTGTAGTGAGTTTGACATTAGCAAGGGTGCCAA 240 3 AY231130 AATTCTGCAATTGGTGCAAATTGGTAGCGCTGAAGTTGGAAGGACCTTCCTGGAGGGTAA 300 3 AY231130 CAGATTTGTCCGTGCGAACATATTTGAGATCATCCGGAAAACCATGGTTGGTCGTCTAGG 360 3 AY231130 ATATGATTTCGAGAGTGAACTATGGGTCTGTCATAACTGCAACAGAACTTCTGAAAAATA 420 3 AY231130 CCTCAAGAAATGTGACTGCGGGGAAAAATATTATTACTCTGAAAGAAACCTGATGAAAAC 480 3 AY231130 AATGAACGACCTGACGTATCAATTTGACATGACACCATCAGAGATCAACTCCGTCGATTT 540 3 AY231130 TGATTACCTTGCTGATGCAGTGGATTATGCTGAACGGTCAGTCCAGAAATCTCAAGTTCC 600 3 AY231130 GGAACCTGTGGAACTTGTAGTGGTGGAACCAATTGCGGCTAGTGAGGAAGGTACTCTAAT 660 3 AY231130 GGTTTCTGAACCAGAAGTTGTACCTGCAACTACCAAAATTGAAGATGCATGGACAATACA 720 3 AY231130 GATTGGGGAAATTCCTGTCCCACTTGTCGTCATCAAAGAGACGCCAGTTATCAGTGGTGT 780 3 AY231130 GAACGGAACTTTGAACTCAACTGGTTTCTCACTTGAAGCTGAGATTACAAAACTGGTGGA 840 3 AY231130 GAAGGAAGTCTCTCAAGAAGAGGTTAAAGAAGCAGTGCATCTGGCACTCGAAGTTGGTAA 900 3 AY231130 CGAGATTGCTGAGAAGAAGCCTGAACTCAAGCTAATACCATACTGGAGTGCTAGCCTTGA 960 3 AY231130 GTTGCACAAGAGAATTCGTAAGCATAAGGAGCATGCTAAGATTGCAGCAATTCAAGTCCA 1020 3 AY231130 GAAGGAAAGAGAAAAGAACCAAAAGATATTCTCAATTCTAGAGTCGAAGCTCAACTTAAA 1080 3 AY231130 ATCCAGGAGGAGAAATCAAACTGTGGTCTGTGACAAAAGAGGCACACTTAAATGGAAAAC 1140 3 AY231130 CCGGCAAGGTTACAAGAAGAGTAGACTAATGCAACAAGCGAGTGACTCTGTTGTCACTCA 1200 81 3 AY231130 GATTTACTGTGATTTTGGTTGTGAACCTCAATATTCTGAACCTCACTCTTCTGGCATTAA 1260 3 AY231130 AATAGCGACATCTAAGAAGGTCCACAAACCGCGCAAGCAATCGAGGATTGTTGGTAATAA 1320 3 AY231130 CAAGATAAATTATGTCATGAAACATCTATGTGACACAATTATCGAAAGAAGCATTCCTGT 1380 3 AY231130 AGAGCTTGTTACAAAGCGATGCAAGAGAAGGATTCTTCAGAAGAAAGGTAGAAGCTATGT 1440 3 AY231130 GCAGTTGAGGCACATGGATGGCATTCGTGCACAACAAGATGTGAGCAGCTCGCCTGATAT 1500 3 AY231130 GGAGCGGTTATTCACGCAATTTTGCAAATTTTTAGTTGGGCACAAACCACTCAAATCTGA 1560 3 AY231130 AAGTCTGACGTTTGGTTCTAGTGGTCTGATTTTTAAGCCAAAGTTTGCCGCCAATGTGGG 1620 3 AY231130 GCGATATTTTGGTGATTATTTCGTTGTTCGAGGACGTCTTGAAGGTAAGCTATTTGATGG 1680 3 AY231130 TAGATCAAAACTAGCGAGATCGATCTATGCCAGGATGGAGCAATACAATGATGTGGCCGA 1740 3 AY231130 AAAATTTTGGCTCGGTTTTAATAGGGCTTTTCTACGGCATAGAAAACCAACGGATCACAC 1800 3 AY231130 TTGCACGTCTGATATGGATGTTACAATGTGTGGGGAGGTAGCAGCTCTGGCAACTATAAT 1860 3 AY231130 TTTGTTTCCGTGCCACAAAATAACTTGCAACACTTGCATGAGCAGAGTGAAGGGAAGAGT 1920 3 AY231130 AATTGACGAAGTTGGTGAGGATTTGAATTCTGAGCTTGAACGTTTACGTGAAACTCTTTC 1980 3 AY231130 TTCATATGGAGGGTCATTCGGACATGTTTCAACATTACTTGATCAAATGAATAGAGTTTT 2040 3 AY231130 GAATGCGCGGAACACAAACGATGGAGCTTTTAAAGAGATTGCGAAGAAGATTGATGCAAA 2100 3 AY231130 GAAAGAGAGTCCTTGGACTCACATGACAACTATCAACAACACGCTCATCAAAGGTTCGCT 2160 3 AY231130 AGCAACTGGTTACGAATTTGAAAGAGCGTCTGATAGTCTCCTAGAGATTGTGAGATGGCA 2220 3 AY231130 TCTCAAAAGGACAGAATCAATAAAAGCTGGCAGCGTTGAGAGTTTTAGAAATAAGCGTTC 2280 3 AY231130 TGGGAAAGCTCACTTCAACCCAGCTCTTACGTGTGATAATCAGTTGGATAGGAATGGTAA 2340 3 AY231130 TTTCTTATGGGGTGAAAGGCAATACCACGCCAAAAGATTCTTTGCCAACTATTTCGAGAA 2400 3 AY231130 GATTGATCATAGTAAGGGTTATGAGTACTATAGTCAACGCCAAAACCCAAATGGTACCAG 2460 3 AY231130 GAAAATTGCCATTGGTAATTTAGTATTTTCAACAAATTTGGATAGATTTCGGCAGCAAAT 2520 3 AY231130 GGTTGAACACCACATTGATCAAGGACCAATCACTCGTGAGTGCATCGCATTGCGCAATAA 2580 82 3 AY231130 CAATTATGTTCATGTATGTAGCTGTGTAACTTTAGATGATGGAACTCCAGCAACAAGTGA 2640 3 AY231130 GTTGAAGACTCCTACCAAGAATCATATCGTTCTTGGTAATTCTGGTGATCCCAAGTACGT 2700 3 AY231130 TGACTTGCCGACTCTTGAGTCTGATTCAATGTACATAGCCAAAAAAGGTTATTGCTACAT 2760 3 AY231130 GAACATATTTTTAGCGATGCTCATAAATATACCTGAGAATGAGGCGAAAGATTTCACAAA 2820 3 AY231130 GAGAGTTCGCGACCTCGTGGGTTCAAAACTTGGAGAGTGGCCAACGATGTTGGATGTCGC 2880 3 AY231130 AACATGCGCAAATCAGCTGATAATCTTTCATCCTGACGCAGCCAATGCAGAATTGCCGCG 2940 3 AY231130 AATTTTAGTGGACCATCGACAGAAAACAATGCATGTTATTGACTCTTTTGGTTCCGTGGA 3000 3 AY231130 TTCTGGATATCATATGTTGAAGGCAAACACAGTTAATCAGCTAATCCAATTCGCTAGAGA 3060 3 AY231130 GCCACTCGATAGTGAAATGAAGCACTACATCGTTGGTGGAGAGTTTGACCCAACTACTAA 3120 3 AY231130 CTGCTTGCATCAGTTGATTCGTGTCATCTACAAGCCTCATGAACTCCGGAACTTGCTCAG 3180 3 AY231130 GAATGAACCGTACCTGATTGTGATTGCATTGATGTCACCAAGTGTACTTTTGACTTTGTT 3240 3 AY231130 CAATAGTGGTGCGATTGAGCACGCGTTGAATTACTGGATCAAAAGAGATCAAGATGTTGT 3300 3 AY231130 TGAGGTCATTGTTTTGGTGGAGCAATTATGCAGGAAAGTTACACTTGCTAGAACAATCCT 3360 3 AY231130 GGAGCAATTTAATGAGATTCGTCAAAATGCGAGAGATATACATGAGCTAATGGATCGAAA 3420 3 AY231130 CAATAAGCCTTGGATTTCATATGATCGCTCATTGGAACTATTGAGTGTGTATGCGAATTC 3480 3 AY231130 GCAGTTGACGGATGAAGGTCTACTCAAGCAAGGATTTTCAACATTAGATCCTAGGTTGCG 3540 3 AY231130 TGAAGCTGTGGAAAAAACCTACGCCACTCTTTTACAGGAAGAATGGCGTGCGTTAAGTTT 3600 3 AY231130 GTTTCAAAAGTTGCACTTAAGGTACTTTGCGTTCAAATCACAACCGTCTTTTTCCGAGTA 3660 3 AY231130 TTTAAAGCCAAAAGGGCGCGCAGATTTAAAAATTGTATACGACTTCTCACCGAAGTATTG 3720 3 AY231130 TGTACACGAGGTCGGAAAGGCGTTTCTACAACCAGTCAGGGCTGGGGCTAAAATTGCATC 3780 3 AY231130 GCGAGTCATTAATGGTTGCGGAACTTTCATTCGGAAGAGTGCCGCAAAAGGCTGTGCTTA 3840 3 AY231130 CATTTTTAAGGATCTTTTCCAGTTTGTACATGTAGTGTTAGTTTTAAGCATTTTATTACA 3900 3 AY231130 GATCTTTAGGAATGCGCAAGGAATTGCCACAGAGCATATACAATTGAAGCAGGCTAAGGC 3960 83 3 AY231130 AGAAATGGAAAGACAGAAAGATTTTGATCGTCTGGAGGCTCTATACGCTGAATTGTGCAT 4020 3 AY231130 TAAGAGCGGTGAGCAACCAACTGCTGAAGAATTTATTGATTTTGTGAAGGAGCGTGAGCC 4080 3 AY231130 AAGGCTTAAGGATCAAGCTTACAATTTAATACACATACCAGTGATTCATCAAGCAAAATC 4140 3 AY231130 GGACAATGAGAAAAAGCTCGAGCAAGTCATTGCATTCATTACATTAATTTTAATGATGAT 4200 3 AY231130 CGATGTTGATAAGAGTGATTGTGTCTATAGAATCCTAAATAAATTTAAAGGCGTGATAAA 4260 3 AY231130 ATCTTGCGATGCAAATGTTTATCACCAGTCTTTAGATGACATTCGGGATTTCTATGAAGA 4320 3 AY231130 CAAGCAGTTGACTATTGATTTCGATATCACTGGAGAAAATCAGATCAATCGAGGACCTAT 4380 3 AY231130 AGACGTTACGTTTGAGAAATGGTGGGACAATCAATTGTCCAATAACAACACAATTGGTCA 4440 3 AY231130 TTATCGAATCGGGGGAACGTTTGTTGAATTTTCACGAAGTACTGCAGCAACTGTGGCTAG 4500 3 AY231130 TGAGATAGCTCATAGTCCTGATCGTGAGTTTCTTGTTCGCGGAGCTGTTGGTAGTGGTAA 4560 3 AY231130 ATCAACGAATCTTCCTTTCTTGCTTAGTAAGCATGGTAGCGTGTTGTTAATAGAGCCCAC 4620 3 AY231130 CAGACCTCTTTGTGAGAACGTCTGTAAGCAATTACGTGGTGAACCGTTCCATTGTAATCC 4680 3 AY231130 AACCATTCGCATGCGTGGATTAACAGCTTTTGGTTCTACCAACATCACAATAATGACAAG 4740 3 AY231130 CGGATTTGCTTTACATTATTATGCTCACAATGTTCAACAATTGAGACTCTTTGATTTCAT 4800 3 AY231130 AATCTTTGATGAATGTCATGTCATAGATAGCCAAGCCATGGCTTTCTATTGTCTGATGGA 4860 3 AY231130 GGGGAACGCTATTGAGAAAAAGATCCTTAAAGTTTCTGCTACGCCACCTGGGCGTGAGGT 4920 3 AY231130 TGAATTTTCAACACAATTCCCAACAAAGATTGTAACGGAACAGTCCATAAGCTTTAAGCA 4980 3 AY231130 GTTGGTTGATAACTTTGGTACTGGTGCGAATAGTGATGTGACCGTTTTTGCTGATAACAT 5040 3 AY231130 ATTAGTTTATGTTGCCAGTTACAATGAAGTAGATCAACTAAGTAAGCTCTTATCCGATAA 5100 3 AY231130 AGGCTACTTAGTCACTAAAATCGATGGAAGAACGATGAAAGTTGGAAAAACTGAAATTTC 5160 3 AY231130 CACTAGTGGCACAAAATCCAAGAAGCATTTCATAGTTGCCACGAATATCATTGAGAATGG 5220 3 AY231130 CGTCACACTTGACATAGAAGCCGTTATAGATTTTGGGATGAAAGTTGTACCTGAGATGGA 5280 3 84 AY231130 TTCAGATAATCGCATGATTCGGTACTCAAAGCAAGCGATCAGTTTTGGAGAGAGAATTCA 5340 3 AY231130 AAGACTTGGCCGAGTGGGAAGACATAAAGAGGGGATTGCACTTAGAATTGGGCACACAGA 5400 3 AY231130 GAAAGGTATCCAAGAAATTCCAGAGATGGCAGCTACTGAAGCAGCTTTTCTGAGCTTCAC 5460 3 AY231130 GTATGGCTTGCCTGTTATGACTCACAATGTAGGGTTAAGCTTGCTTAAAAATTGCACTGT 5520 3 AY231130 GAGACAAGCACGCACAATGCATCAGTATGAACTGAGCCCATTCTTTACACAAAATTTGGT 5580 3 AY231130 AAATTTTGATGGGACAGTGCACCCCAAGATTGATATTCTGTTACGTCCCTATAAGCTGAG 5640 3 AY231130 AGATTGTGAAGTCAGATTAAGTGAAGCAGCAATTCCGCATGGGGTACAGTCTATTTGGTT 5700 3 AY231130 GTCTGCGCGGGATTATGAAGCAGTTGGAGGTCGTCTTTGCTTGGAGAGCGATGTTCGAA 5760 3 AY231130 ACCATTCCTCATTAAGGATGTTCCTGAGCGGTTATACAAAGAATTGTGGGATATCGTGCA 5820 3 AY231130 GACCTACAAGCGTGACTTTACATTTGGGCGAATTAATTCTGTGTCTGCTGGGAAAATTGC 5880 3 AY231130 GTACACATTAAGGACTGATGTGTATTCTATTCCTAGAACTCTCATAACGATTGACAAGCT 5940 3 AY231130 GATTGAGAGTGAAAACATGAAGCATGCCCATTTTAAAGCCATGACAAGTTGCACTGGCTT 6000 3 AY231130 AAACTCCAGCTTTTCTCTCCTTGGTGTTATAAACACTATCCAGAGTAGATATCTAGTTGA 6060 3 AY231130 TCATTCAGTTGAAAATATCAGGAAACTTCAACTAGCGAAGGCCCAGATTCAACAACTTGA 6120 3 AY231130 AGCTCATGTGCAAGAAAACAATGTTGAAAATTTAATTCAATCTCTTGGTGCTGTTAGAGC 6180 3 AY231130 TGTTTACCATCAAAGTGTTGATGGAGTCAAGCACATAAAGCGAGAGTTGGGCTTGAAGGG 6240 3 AY231130 AATTTGGGATGGTTCATTGATGATCAAGGATGCGATTGTATGCGGTTTCACAATGGCTGG 6300 3 AY231130 CGGTGCGATGCTTTTGTACCAACACTTTCGTGATAGGCTTACAAATGTTCATGTGTTTCA 6360 3 AY231130 CCAAGGTTTCTCTGCGCGACAGAGACAAAAGTTAAGATTTAAGTCAGCAGCAAATGCTAA 6420 3 AY231130 GCTTGGTCGAGAGGTCTATGGAGATGATGGAACAATTGAGCACTACTTTGGAGAGGCGTA 6480 3 AY231130 CACGAAGAAAGGAAACAAGAAAGGAAAGATGCATGGCATGGGCGTTAAAACGAGGAAATT 6540 3 AY231130 TGTTGCGACATATGGATTTAAACCGGAGGATTACTCGTATGTGCGGTACTTGGATCCTTT 6600 3 AY231130 AACAGGTGAGACTTTAGATGAAAGCCCACAGACTGATATCTCAATGGTGCAAGAACATTT 6660 3 85 AY231130 TGGTGAAATTCGGAATAAGTACATGGATTCAGACAGCTTCGACAGGCAAGCTTTGATAGC 6720 3 AY231130 AAACAATACAATTAAGGCCTATTATGTCCGAAACTCCGCGAAGACAGCATTGGAAGTTGA 6780 3 AY231130 TCTGACACCGCACAACCCTCTTAAAGTTTGTGACAATAAGTTGACCATTGCAGGATTTCC 6840 3 AY231130 AGACAGGGAAGCTGAGCTGAGACAAACAGGCCCGCCCAGAACTATTCAAGTCAATCAAGT 6900 3 AY231130 GCCACCACCTTCAAGATCAGTTCATCATGAAGGAAAAAGTCTTTGTCAAGGCATGCGAAA 6960 3 AY231130 TTACAATGGCATAGCTTCTGTAGTTTGCCATTTGAAAAACACATCAGGAAAGGGGAAGAG 7020 3 AY231130 CTTATTTGGAATCGGATATAATTCATTCATCATTACCAACCGACATTTGTTCAAGGAGAA 7080 3 AY231130 TAATGGTGAACTTATAGTGAAATCCCAACACGGTAAGTTTGTCGTCAAGAACACCACGAC 7140 3 AY231130 GCTCCGAGTTGCTCCAGTTGGTAAGACTGATCTTTTAATTATTCAAATGCCGAAAGATTT 7200 3 AY231130 TCCTCCATTTCATAGCAGAGCTAGGTTTAGGGCCATGAAAGCTGGGGACAAGGTTTGCAT 7260 3 AY231130 GATAGGTGTTGATTACCAAGAGAATCATATCGCGAGCAAAGTGTCTGAAACTTCTATCAT 7320 3 AY231130 AGTGAGGGCACGGGAGATTTTGGATGCCACTGGATTTCCACGAATGACGGTGATTGCGG 7380 3 AY231130 CAATCCATTAGTTAGTGTTTCAGATGGTTTTATTGTTGGCTTGCATAGTCTGTCGACATC 7440 3 AY231130 AACTGGAGATCAAAATTTCTTCGCTAAAATACCCGCACAATTTGAAGAAAAGGTCCTGAA 7500 3 AY231130 GAAGATTGATGATTTAACTTGGAGCAAACACTGGAGCTATAATATTAATGAACTGAGTTG 7560 3 AY231130 GGGGGCTCTCAAAGTGTGGGAAAGTCGACCCGAAGCAATTTTTAACGCGCAAAAGGAAGT 7620 3 AY231130 TAATCAATTGAATGCCTTCGAGCAAAGTGGTAGTCGTTGGCTCTTTGACAAACTACACGG 7680 3 AY231130 CAATTTGAAAGGAGTAAGTTCCGCTCCTAGCAATTTGGTGACAAAGCACGTTGTTAAAGG 7740 3 AY231130 TATTTGTCCTCTCTTTAGAAACTATCTCGAGTGTGATAAAGAGGCGAAAGCTTTCTTTAG 7800 3 AY231130 TCCACTTATGGGTCACTACATGAAGAGTGTTCTGAGCAAGGAAGCATACATTAAGGATTT 7860 3 AY231130 ATTTAAATATTCAAGTGACATTGTCGTTGGAGAAGTCAACCATGATATTTTTGAGGATAG 7920 3 AY231130 TGTCGCGCAAGTTGTCGAACTGTTGAACGATCACGAGTGTTTTGAACTTGAATATATTAC 7980 3 AY231130 AGACAGTGAAGTGATTATTCAGGCCTTGAACATGGATGCAGCTGTTGGAGCCTTGTACAC 8040 86 3 AY231130 AGGAAAGAAAAGGAAATATTTTGAGGGTTCAACAGTGGAGCATAGACAAGCACTTGTGCG 8100 3 AY231130 GAAAAGCTGTGAGCGTCTCTATGAAGGGAGAATGGGAGTCTGGAATGGGTCGCTAAAGGC 8160 3 AY231130 TGAACTGAGACCAGCTGAAAAAGTGCTCGCGAAAAAGACAAGGTCATTTACAGCAGCTCC 8220 3 AY231130 TCTTGACACACTGTTAGGAGCCAAAGTCTGCGTTGATGATTTCAACAACTGGTTCTACAG 8280 3 AY231130 TAAGAACATGGAATGTCCATGGACCGTTGGGATGACAAAATTTTACAAAGGCTGGGATGA 8340 3 AY231130 GTTTCTGAAGAAGTTTCCTGAAGGCTGGGTGTATTGTGATGCAGATGGCTCCCAGTTCGA 8400 3 AY231130 TAGCTCATTGACACCATACTTGTTAAATGCTGTGTTGTCAATCCGGTTATGGGCAATGGA 8460 3 AY231130 GGATTGGGATATTGGAGAGCAAATGCTTAAGAATTTGTATGGGGAAATTACTTACACGCC 8520 3 AY231130 AATATTGACACCGGATGGGACAATCGTCAAGAAGTTCAAAGGAAATAATAGTGGTCAACC 8580 3 AY231130 TTCGACAGTTGTTGATAATACATTAATGGTTTTAATCACAATGTATTACGCACTACGGAA 8640 3 AY231130 GATTGGTTATGATACGAAGGCTCAAGAAGACATGTGTGTATTTTATATCAATGGTGATGA 8700 3 AY231130 CCTCTGTATTGCAATTCACCCGGATCATGAGCATGTTCTTGACTCATTCTCTAGTTCATT 8760 3 AY231130 TGCTGAGCTTGGGCTTAAGTATGACTTCACACAAAGGCACCGGAATAAACAGGATTTGTG 8820 3 AY231130 GTTTATGTCGCATCGAGGTATTCTGATCGATGACATTTACATTCCGAAACTTGAACCTGA 8880 3 AY231130 GCGAATTGTTGCAATTCTTGAATGGGACAAATCTAAGCTTCCAGAGCATCGATTAGAGGC 8940 3 AY231130 AATCACAGCAGCAATGATAGAGTCATGGGGTTATGGTGAGCTAACACACCAAATTCGTAG 9000 3 AY231130 ATTTTATCAATGGGTTCTTGAACAAGCTCCATTCAATGAGTTGGCGAAACAAGGAAGGGC 9060 3 AY231130 CCCATACGTCTCAGAAGTTGGATTAAGAAGATTGTATACGAGTGAACGTGGATCAATGGA 9120 3 AY231130 TGAATTGGAAGCGTATATAGATAAGTACTTTGAGCGTGAGAGGGGAGAATCACCCGAATT 9180 3 AY231130 ACTAGTGTATCATGAATCGAAGAGCACCGATGATTATCAACTTGTTTGTAATAACAATGC 9240 3 AY231130 ACATGTGTTTCATCAGTCAAAAAATGAAGCTGTGGATGCTGGTTTGAATGAAAAACTCAA 9300 3 AY231130 AGAAAAAGAAAAACAGAAAGAAAAAGAAAAACAAAAAGAGAAAGAAAAAGACGATGCTAG 9360 3 AY231130 TGACGGAAATGATGTGTCGACTAGCACAAGAACTGGAGAGAAAGATAGAGATGTCAATGT 9420 87 3 1 CCGAGAATAAAATCATTTACTGACAAGATGATTTT 35 AY231130 CGGGACCAGTGGAACTTTCACGGTTCCGAGAATTAAATCATTCACTGATAAGATGATTCT 9480 ******** ******** ***** ********* * 9444 3 ACCGAAAATTAAAGGAAAAACTGTCCTTAATTTTAAATCATCTTCTTCAGTATAATCCGC 95 AY231130 ACCAAGAATTAAGGGAAAGACTGTCCTTAATTT-AAATCATCTTCTTCAGTATAATCCGC 9539 *** * ****** ***** ************** ************************** 3 AACAAATTGATATCTCAAACACTCGTGCCACTCAAACTCAATTCGAAAAGTGGTATGAGG 155 AY231130 AACAAATTGACATTTCTAACACTCGTGCCACTCAGTCACAATTTGAGAAATGGTATGAGG 9599 ********** ** ** ***************** * ***** ** ** ********** 3 GAGTGAGGAATGATTACGGTCTCAACGATAACGAAATGCAAGTG-ATGTTAAACGGTTTG 214 AY231130 GAGTGAGGAATGATTATGGTCTGAATGATAATGAAATGCAAGTG-ATGCTGAATGGCTTG 9658 **************** ***** ** ***** ************ *** * ** ** *** 3 ATGGTTTGGTGTATTGAAAATGGTACATCTCCAGACTTATCTGGTGTTTGGGTAATGATG 274 AY231130 ATGGTTTGGTGTATCGAGAATGGTACATCTCCAGACATATCTGGTGTTTGGGTTATGATG 9718 ************** ** ****************** **************** ****** 3 GATGGGGAAACACAAGTTGATTATCCCATTAAGCCTTTAATTGAACATGCAACTCCTTCG 334 AY231130 GATGGGGAAATTCAAGTTGACTATCCAATCAAGCCTTTAATTGAGCATGCTACCCCGTCA 9778 ********** ******** ***** ** ************** ***** ** ** ** 3 TTTAGGCAAATCATGGCTCACTTCAGTAACGCGGCAGAGGCATACATCGCAAAGAGGAAT 394 AY231130 TTTAGGCAGATTATGGCTCACTTTAGTAACGCGGCAGAAGCATATATTGCAAAGAGAAAT 9838 ******** ** *********** ************** ***** ** ******** *** 3 395 G AY2311309839 GCCACTGAGAGGTACATGCCGCGGTATGGAATCAAGAGAAATTTGACTGACATTAGCCTC 9898 * 3 AY231130 GCTAGATACGCTTTCGATTTCTATGAGGTTAATTCGAAAACACCTGATAGGGCTCGCGAA 3 AY231130 GCTCACATGCAGATGAAAGCTGCAGCGCTGCGAAACACTAGTCGCAGAATGTTTGGTATG 3 AY231130 GACGGCAGTGTTAGTAATAAGGAAGAAAACACGGAGAGACACACAGTGGAAGATGTCAAT 3 AY231130 AGAGACATGCACTCTCTCCTGGGTATGCGCAACTGAATACTCGCGCTGGTGTGTTTGTCG 3 AY231130 AGCTCGACTCGACCTTGTTTCACCTTATAGTACTAAATAAGCATTAGAATACAGTGTGGC 3 AY231130 TGTGCCACCACTTCTATTTTACAGTGAGGGTAGCCCTCCGTGCTTTTAGTATTATTCGAG 3 AY231130 TTCTCTGAGTCTCCATACAGTGTGGGTGGCCCACGTGTTATTTGAGCCTCTTAGAATGAG 3 AY231130 AG ALIGN 395 NU . GTTGCACAAGAGAATTCGTAAGCATAAGGAGCATGCTAAGATTGCAGCAATTCAAGTCCA 102 0 3 AY231130 GAAGGAAAGAGAAAAGAACCAAAAGATATTCTCAATTCTAGAGTCGAAGCTCAACTTAAA 108 0 3 AY231130 ATCCAGGAGGAGAAATCAAACTGTGGTCTGTGACAAAAGAGGCACACTTAAATGGAAAAC. ACAGTGTGGGTGGCCCACGTGTTATTT ALIGN 396 NU 80 Phụ lục 13 Kết quả so với genome của PRSV trên Genebank CLUSTAL X (1.83) multiple sequence alignment 3 AY231130 1 AAATAAAACATCTCAACACAACATAATCAAAAGCATTTCAACAAACATCAACTTATCTCA. 79 Phụ lục 12 Kết quả so với trình tự của CP-PRSV trên Genebank CLUSTAL X (1.83) multiple sequence alignment 3 AJ012649 TCCAAGAATGAAGCTGTGGATGCTGGTTTGGATGAAAAACTCAAAGAAAAAGAAAAACAG

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