Salmonella Differential Agar 1535 Salinibacter ruber Medium (DSMZ Medium 936) Composition per liter: NaCl 195.0g MgSO 4 ·7H 2 O 49.5g MgCl 2 ·6H 2 O 34.6g KCl 5.0g CaCl 2 ·2H 2 O 1.25g Yeast extract 1.0g NaBr 0.625g NaHCO 3 0.25g pH 7.2 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation of Salinibacter ruber. Salinivibrio costicola Subspecies vallismortis Medium (DSMZ Medium 597) Composition per liter: NaCl 25.0g MgSO 4 ·7H 2 O 9.6g MgCl 2 ·6H 2 O 7.0g Glucose 5.0g KCl 3.8g Yeast extract 1.0g CaCl 2 ·2H 2 O 0.5g K 2 HPO 4 ·3H 2 O 0.4g NaHCO 3 solution 100.0mL pH 7.0 ± 0.2 at 25°C NaHCO 3 Solution: Composition per 100.0mL: NaHCO 3 3.0g Preparation of NaHCO 3 Solution: Add NaHCO 3 to distilled/de- ionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except NaHCO 3 solu- tion, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 100.0mL NaHCO 3 solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of Salinivibrio costicola subsp. vallismortis. Salmonella Chromogen Agar (Rambach Equivalent Agar) Composition per liter: Agar 15.0g Peptone 5.0g NaCl 5.0g Yeast extract 2.0g Meat extract 1.0g Sodium deoxycholate 1.0g pH 7.3 ± 0.2 at 25°C Source: This medium is available from Fluka, Sigma-Aldrich. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure– 121°C. Pour into sterile Petri dishes. Use: A differential diagnostic agar for the detection of Salmonella in food, including the isolation and enumeration of Salmonella from bivalves. Salmonella Chromogenic Agar (OSCM) Composition per liter: Chromogenic mix 28.0g Agar 12.0g Special peptone 10.0g Selective supplement solution 10.0mL pH 7.2 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Oxoid Unipath. Selective Supplement Solution: Composition per 10.0mL: Cefsulodin 12.0mg Novobiocin 5.0mg Preparation of Selective Supplement Solution: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thor- oughly. Filter sterilize. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0mL Mix thoroughly. Gently heat while stirring and bring to boiling. Do not autoclave. Cool quickly to 50°C. Mix thoroughly. Pour into sterile Petri dishes. Use: For the identification of Salmonella species and differentiation of Salmonella spp. other organisms in the family Enterobacteriaceae. This medium combines two chromogens for the detection of Salmo- nella spp., 5-bromo-6-chloro-3-indolyl caprylate (Magenta-caprylate) and 5-bromo-4-chloro-3-indolyl-β- D galactopyranoside (X-gal). X-gal is a substrate for the enzyme β-D-galactosidase. Hydrolysis of the chro- mogen, Magenta-caprylate, by lactose-negative Salmonella species results in magenta colonies. The addition of the selective supplement solution increases the selectivity of the medium. Novobiocin inhibits Proteus growth and cefsulodin inhibits growth of pseudomonads. Salmonella Differential Agar (RajHans Medium) Composition per liter: Agar 12.0g Propylene glycol 10.0g Peptone, special 8.0g B.C. indicator 2.0g Yeast extract 2.0g Sodium deoxycholate 1.0g pH 7.3 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Mix to completely dissolve components. Do not autoclave. Cool to 50°C. Mix thoroughly. Pour into sterile Petri dishes. © 2010 by Taylor and Francis Group, LLC 1536 Salmonella Differential HiVeg Agar, Modified Use: A selective chromgenic medium used for the isolation and differ- entiation of Salmonella species from the members of Enterobacteri- aceae, especially Proteus species. Salmonella Differential HiVeg Agar, Modified (Salmonella Differential Agar, Modified, HiVeg) Composition per liter: Propylene glycol 10.0g Plant special peptone 8.0g NaCl 5.0g Yeast extract 3.0g B.C. indicator 2.0g Synthetic detergent 1.0g pH 7.3 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Mix to completely dissolve components. Do not autoclave. Cool to 50°C. Mix thoroughly. Pour into sterile Petri dishes. Use: A selective chromogenic medium used for the isolation and dif- ferentiation of Salmonella species from the members of Enterobacteri- aceae, especially Proteus species. Salmonella HiVeg Agar, ONOZ Composition per liter: Agar 15.0g Sucrose 13.0g Lactose 11.5g Na 3 -citrate·5H 2 O 9.3g Plant peptone 8.625g Plant extract No. 1 6.0g L-Phenylalanine 5.0g Na 2 S 2 O 3 ·5H 2 O 4.25g Yeast extract 3.0g Synthetic detergent No. 1 2.0g Na 2 HPO 4 ·2H 2 O 1.0g Ferric citrate 0.5g Metachrome Yellow 0.47g MgSO 4 0.4g Aniline Blue 0.25g Neutral Red 0.022g Brilliant Green 1.66mg pH 7.4 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Gently heat and bring to boiling. Do not autoclave. Cool to 50°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance Salmonella spp. from clini- cal specimens. Salmonella Medium Composition per liter: Pancreatic digest of casein 10.0g NaCl 5.0g pH 7.4 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation and maintenance of Escherichia coli and Sal- monella choleraesuis. Salmonella Rapid Test Elective Medium Composition per liter: Tryptone 10.0g Na 2 HPO 4 9.0g Sodium chloride 5.0g Casein 5.0g KH 2 PO 4 1.5g Malachite Green 0.0025g pH 6.5 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the Oxoid Salmonella Rapid Test which is for the presump- tive detection of motile Salmonella in foods and environmental sam- ples. Salmonella Rapid Test Elective Medium, 2X Composition per liter: Tryptone 20.0g Na 2 HPO 4 18.0g Sodium chloride 10.0g KH 2 PO 4 3.0g Casein 10.0g Malachite Green 0.005g pH 6.5 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Use: Use as described in the Oxoid Salmonella Rapid Test which is for the presumptive detection of motile Salmonella in foods and environ- mental samples. Salmonella Shigella Agar (SS Agar) Composition per liter: Agar 13.5g Lactose 10.0g Bile salts 8.5g Na 2 S 2 O 3 8.5g Sodium citrate 8.5g Beef extract 5.0g Pancreatic digest of casein 2.5g Peptic digest of animal tissue 2.5g Ferric citrate 1.0g Neutral Red 0.025g Brilliant Green 0.33mg pH 7.0 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems and Oxoid Unipath. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Do not autoclave. Cool to 45°–50°C. Pour © 2010 by Taylor and Francis Group, LLC Salt Meat Broth 1537 into sterile Petri dishes in 20.0mL volumes. Allow the surface of the plates to dry before inoculation. Use: For the selective isolation and differentiation of pathogenic enteric bacilli, especially those belonging to the genus Salmonella. This medium is not recommended for the primary isolation of Shigella species. Lactose-fermenting bacteria such as Escherichia coli or Kleb- siella pneumoniae appear as small pink or red colonies. Lactose-non- fermenting bacteria—such as Salmonella species, Proteus species, and Shigella species—appear as colorless colonies. Production of H 2 S by Salmonella species turns the center of the colonies black. Salmonella Shigella Agar, Modified (SS Agar, Modified) Composition per liter: Agar 12.0g Lactose 10.0g Sodium citrate 10.0g Na 2 S 2 O 3 8.5g Bile salts 5.5g Beef extract 5.0g Peptone 5.0g Ferric citrate 1.0g Neutral Red 0.025g Brilliant Green 0.33mg pH 7.3 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Oxoid Unipath. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Do not autoclave. Cool to 45°–50°C. Pour into sterile Petri dishes in 20.0mL volumes. Allow the surface of the plates to dry before inoculation. Use: For the selective isolation and differentiation of pathogenic enteric bacilli, especially those belonging to the genus Salmonella. This medium provides better growth of Shigella species. Lactose-fer- menting bacteria such as Escherichia coli or Klebsiella pneumoniae appear as small pink or red colonies. Lactose-nonfermenting bacte- ria—such as Salmonella species, Proteus species, and Shigella spe- cies—appear as colorless colonies. Production of H 2 S by Salmonella species turns the center of the colonies black. Salmonella Shigella Deoxycholate Agar See: SS Deoxycholate Agar Salt Agar Composition per liter: NaCl 58.4g Agar 15.0g Proteose peptone 5.0g Pancreatic digest of casein 5.0g pH 6.9 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of Marinococcus halophilus. Salt Broth, Modified Composition per liter: NaCl 65.0g Enzymatic digest of animal tissue 10.0g Heart digest 10.0g Glucose 1.0g Bromcresol Purple 0.016g pH 7.2 ± 0.2 at 25°C Source: Available as a prepared medium from BD Diagnostic Sys- tems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation and differentiation of the enterococcal group D streptococci from nonenterococcal group D streptococci based on salt tolerance. Salt Colistin Broth Composition per liter: NaCl 20.0g Peptone 10.0g Yeast extract 3.0g Colistin solution 10.0mL pH 7.4 ± 0.2 at 25°C Colistin Solution: Composition per 10.0mL: Colistin methane sulfonate 500,000U Preparation of Colistin Solution: Add colistin methane sulfonate to distilled/deionized water and bring volume to 10.0mL. Mix thor- oughly. Filter sterilize. Preparation of Medium: Add components, except colistin solu- tion, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat until dissolved. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add sterile colistin solu- tion. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of halophilic Vibrio species. Salt Malt Agar (SMA1) Composition per liter: NaCl 25.0g Agar 15.0g Malt extract 10.0g Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation of Dendryphiella salina and Dendryphiella arenaria. Salt Meat Broth Composition per liter: NaCl 100.0g Neutral ox-heart tissue 30.0g Beef extract 10.0g Peptone 10.0g pH 7.6 ± 0.2 at 25°C Source: This medium is available as tablets from Oxoid Unipath. © 2010 by Taylor and Francis Group, LLC 1538 Salt Medium Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the isolation and cultivation of staphylococci from specimens with a mixed flora such as fecal specimens, especially during the inves- tigation of staphylococcal food poisoning. Salt Medium Composition per liter: NaCl 58.4g Proteose peptone 5.0g Pancreatic digest of casein 5.0g pH 4.9 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation and maintenance of Marinococcus halophilus. Salt Nutrient Agar Composition per liter: NaCl 25.0g Agar 15.0g Peptone 5.0g Yeast extract 2.0g Beef extract 1.0g Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation of Bacillus species, Pseudomonas beijer- inckii, and other Pseudomonas species. Salt Polymyxin Broth (SPB) Composition per liter: NaCl 20.0g Pancreatic digest of casein 10.0g Yeast extract 3.0g Polymyxin B 250,000U pH 8.8 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 8.8. Distribute into tubes or flasks. Autoclave for 10 min at 10 psi pressure– 115°C. Use: For the isolation and cultivation of Vibrio species from foods. Salt Polymyxin HiVeg Broth Base with Polymyxin Composition per liter: NaCl 20.0g Plant hydrolysate 10.0g Yeast extract 3.0g Selective supplement 10.0mL pH 8.8 ± 0.2 at 25°C Source: This medium, without selective supplement, is available as a premixed powder from HiMedia. Selective Supplement: Composition per 10.0mL: Polymyxin B sulfate 100,000U Preparation of Selective Supplement: Add polymycin B sulfate to distilled/deionized water and bring volume to 10.0mL. Mix thor- oughly. Filter sterilize. Preparation of Medium: Add components, except selective sup- plement, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Mix thoroughly. Adjust pH to 8.8. Distribute into tubes or flasks. Autoclave for 10 min at 10 psi pressure–115°C. Cool to 50°C. Aseptically add 10.0mL sterile selective supplement. Mix thoroughly. Pour into sterile Petri dishes or aseptically distribute into tubes. Use: For the isolation and cultivation of Vibrio species from foods. Salt Tolerance Medium Composition per liter: Beef heart, infusion from 500.0g NaCl 65.0g Tryptose 10.0g Glucose 1.0g Indicator solution 1.0mL pH 7.4 ± 0.2 at 25°C Indicator Solution: Composition per 100.0mL: Bromcresol Purple 1.6g Ethanol (95% solution) 100.0mL Preparation of Indicator Solution: Add Bromcresol Purple to ethanol. Mix thoroughly. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation of salt-tolerant Streptococcus species and other salt-tolerant Gram-positive cocci. For the differentiation of Gram-positive cocci based on salt tolerance. Salt Tolerance Medium Composition per liter: NaCl 60.0g Peptone 5.0g Yeast extract 2.0g Beef extract 1.0g pH 7.4 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation and differentiation of Aeromonas and Ple- siomonas species based on salt tolerance. Salt Tolerance Medium Composition per liter: Beef heart, solids from infusion 500.0g NaCl 65.0g Tryptose 10.0g pH 7.4 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For testing the salt tolerance of a variety of microorganisms. © 2010 by Taylor and Francis Group, LLC Saprospira grandis Medium 1539 Salt Tolerance Medium, Gilardi Composition per liter: NaCl 65.0g Pancreatic digest of casein 15.0g Agar 15.0g Papaic digest of soybean meal 5.0g pH 7.3 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Do not overheat. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of salt-tolerant, nonferment- ing Gram-negative bacteria. For the differentiation of nonfermenting Gram-negative bacteria based on salt tolerance. Salt Tolerance Medium, Tatum Composition per liter: NaCl 65.0g Peptone 5.0g Yeast extract 2.0g Beef extract 1.0g pH 7.4 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation of salt-tolerant, nonfermenting Gram-nega- tive bacteria. For the differentiation of nonfermenting Gram-negative bacteria based on salt tolerance. Sanfrancisco Medium Composition per liter: Rye bran or wheat bran 50.0g Fresh baker's yeast 21.0g Pancreatic digest of casein 10.0g Fructose 7.0g Glucose 7.0g Maltose 7.0g Yeast extract 7.0g Diammonium citrate 5.0g Sodium acetate·3H 2 O 5.0g KH 2 PO 4 ·3H 2 O 2.5g Meat extract 2.0g Sodium gluconate 2.0g Tween™ 80 1.0g L-Cysteine·HCl 0.5g MgSO 4 ·7H 2 O 0.2g MnSO 4 ·4H 2 O 0.05g FeSO 4 ·7H 2 O 0.01g pH 5.4 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 5.4. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C. Use: For the cultivation of Lactobacillus sanfrancisco. SAP 1 Agar Composition per liter: Agar 15.0g Pancreatic digest of casein 5.0g Yeast extract 5.0g Artificial seawater 1.0L pH 7.2 ± 0.2 at 25°C Artificial Seawater: Composition per liter: NaCl 24.7g MgSO 4 ·7H 2 O 6.3g MgCl 2 ·6H 2 O 4.6g CaCl 2 1.0g KCl 0.7g NaHCO 3 0.2g Preparation of Artificial Seawater: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Medium: Add solid components to 1.0L of artifi- cial seawater. Mix thoroughly. Gently heat and bring to boiling. Dis- tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the isolation and cultivation of Cytophaga species, Herpeto- siphon species, Saprospira species, and Flexithrix species. SAP 2 Agar Composition per liter: Agar 15.0g Pancreatic digest of casein 1.0g Yeast extract 1.0g Artificial seawater 1.0L pH 7.2 ± 0.2 at 25°C Artificial Seawater: Composition per liter: NaCl 24.7g MgSO 4 ·7H 2 O 6.3g MgCl 2 ·6H 2 O 4.6g CaCl 2 1.0g KCl 0.7g NaHCO 3 0.2g Preparation of Artificial Seawater: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Medium: Add solid components to 1.0L of artifi- cial seawater. Mix thoroughly. Gently heat and bring to boiling. Dis- tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the isolation and cultivation of Cytophaga species, Herpeto- siphon species, Saprospira species, and Flexithrix species. Saprospira grandis Medium Composition per 1010.0mL: Pancreatic digest of casein 5.0g Yeast extract 5.0g Ca(NO 3 ) 2 ·4H 2 O 0.1g K 2 HPO 4 0.02g Seawater, filtered 1.0L Trace elements 10.0mL pH 7.0 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC 1540 Sarcina maxima Medium Trace Elements: Composition per liter: FeSO 4 ·7H 2 O 0.5mg ZnSO 4 ·7H 2 O 0.3mg H 3 BO 3 0.1mg CoCl 2 ·6H 2 O 0.1mg CuSO 4 ·5H 2 O 0.1mg MnSO 4 ·4H 2 O 0.1mg Na 2 MoO 4 ·2H 2 O 0.1mg Preparation of Trace Elements: Add components to distilled/de- ionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Medium: Combine components. Mix thoroughly. Adjust pH to 7.0. Filter sterilize. Use: For the cultivation of Saprospira grandis. Sarcina maxima Medium Composition per liter: Glucose 10.0g Peptone 10.0g Yeast extract 5.0g L-Cysteine·HCl solution 10.0mL pH 6.0 ± 0.2 at 25°C L-Cysteine·HCl Solution: Composition per 10.0mL: L-Cysteine·HCl 0.5g Preparation of L-Cysteine·HCl Solution: Add L-cysteine·HCl to distilled/deionized water and bring volume to 10.0mL. Mix thorough- ly. Filter sterilize. Preparation of Medium: Add components, except L-cysteine·HCl solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add sterile L- cysteine·HCl solution. Mix thoroughly. Aseptically distribute into ster- ile tubes or flasks. Use: For the cultivation of Sarcina maxima. Sarcina Medium (DSMZ Medium 21) Composition per liter: Glucose 30.0g Peptone 5.0g Yeast extract 5.0g Distilled water 1000.0mL pH 6.0 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.0. Gently heat and bring to boiling. Distribute into tubes or flasks. Auto- clave for 15 min at 15 psi pressure–121°C. Use: For the cultivation and maintenance of Sarcina ventriculi and Sarcina maxima. Sarcina ventriculi Growth Medium Composition per liter: Glucose 30.0g Peptone 5.0g Yeast extract 5.0g pH 6.0 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes in 10.0mL volumes. Autoclave for 20 min at 15 psi pressure–121°C. Use: For the cultivation and maintenance of Sarcina maxima and Sarcina ventriculi. Sauton’s Fluid Medium Base Composition per liter: L-Asparagine 1.33g Polysorbate 80 0.833g Citric acid 0.66g K 2 HPO 4 0.177g NaH 2 PO 4 0.056g NaCl 0.035g Ferric ammonium citrate (brown) 0.0167g Glycerol 20.0mL pH 7.2 ± 0.2 at 25°C Source: This medium is available from HiMedia. Preparation of Medium: Add glycerol to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Add remaining compo- nents. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the enumeration of mycobacteria. Sauton’s Medium Composition per liter: L-Asparagine 4.0g Citric acid 2.0g K 2 HPO 4 0.5g MgSO 4 0.5g Triton ® WR 1339 0.25g Ferric ammonium citrate 0.05g Glycerol 40.0mL Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation of Mycobacterium tuberculosis strain Bacille Calmette-Guèrin (BCG) for vaccine production. SBG Enrichment Broth (Selenite Brilliant Green Enrichment Broth) Composition per liter: D-Mannitol 5.0g Peptone 5.0g Yeast extract 5.0g Na 2 SeO 3 ·5H 2 O 4.0g K 2 HPO 4 2.65g KH 2 PO 4 1.02g Sodium taurocholate 1.0g Brilliant Green 5.0mg pH 7.2 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring © 2010 by Taylor and Francis Group, LLC SB/SW Medium 1541 to boiling. Continue boiling for 5–10 min. Do not autoclave. Distribute into sterile tubes or flasks. Use: For the selective isolation of Salmonella species, especially from eggs and egg products. SBG Sulfa Enrichment Composition per liter: D-Mannitol 5.0g Peptone 5.0g Yeast extract 5.0g Na 2 SeO 3 ·5H 2 O 4.0g K 2 HPO 4 2.65g KH 2 PO 4 1.02g Sodium taurocholate 1.0g Sodium sulfapyridine 0.5g Brilliant Green 5.0mg pH 7.2 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Continue boiling for 5–10 min. Do not autoclave. Distribute into sterile tubes or flasks. Use: For the selective isolation of Salmonella species, especially from eggs and egg products. SB/SW Medium Composition per liter: NaCl 1.0g KCl 0.5g MgCl 2 ·6H 2 O 0.4g NH 4 Cl 0.25g KH 2 PO 4 0.2g CaCl 2 ·2H 2 O 0.15g Resazurin 1.0mg Sodium crotonate solution 50.0mL NaHCO 3 solution 20.0mL Na 2 S·9H 2 O solution 10.0mL Seven vitamin solution 10.0mL Sodium dithionite solution 10.0mL Trace elements solution SL-10 1.0mL pH 7.2 ± 0.2 at 25°C Trace Elements Solution SL-10: Composition per liter: FeCl 2 ·4H 2 O 1.5g CoCl 2 ·6H 2 O 190.0mg MnCl 2 ·4H 2 O 100.0mg ZnCl 2 70.0mg Na 2 MoO 4 ·2H 2 O 36.0mg NiCl 2 ·6H 2 O 24.0mg H 3 BO 3 6.0mg CuCl 2 ·2H 2 O 2.0mg HCl (25% solution) 10.0mL Preparation of Trace Elements Solution SL-10: Prepare and dis- pense under 80% N 2 + 20% CO 2 . Add FeCl 2 ·4H 2 O to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring vol- ume to 1.0L. Add remaining components. Mix thoroughly. Sparge with 80% N 2 + 20% CO 2 . Autoclave for 15 min at 15 psi pressure–121°C. Sodium Crotonate Solution: Composition per 50.0mL: Sodium crotonate 1.1g Preparation of Sodium Crotonate Solution: Add sodium croto- nate to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize. Sparge with 80% N 2 + 20% CO 2 . NaHCO 3 Solution: Composition per 20.0mL: NaHCO 3 2.5g Preparation of NaHCO 3 Solution: Add NaHCO 3 to distilled/de- ionized water and bring volume to 20.0mL. Mix thoroughly. Filter ster- ilize. Sparge with 80% N 2 + 20% CO 2 . Na 2 S·9H 2 O Solution: Composition per 10.0mL: Na 2 S·9H 2 O 0.36g Preparation of Na 2 S·9H 2 O Solution: Add Na 2 S·9H 2 O to dis- tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N 2 . Autoclave for 15 min at 15 psi pressure–121°C. Seven Vitamin Solution: Composition per liter: Pyridoxine·HCl 0.3g Thiamine·HCl 0.2g Nicotinic acid 0.2g Calcium DL-pantothenate 0.1g Vitamin B 12 0.1g p-Aminobenzoic acid 80.0mg Biotin 20.0mg Preparation of Seven Vitamin Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sodium Dithionite Solution: Composition per 10.0mL: Sodium dithioninium 0.2g Preparation of Sodium Dithionite Solution: Add sodium dithi- oninium to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N 2 . Autoclave for 15 min at 15 psi pres- sure–121°C. Preparation of Medium: Prepare medium and dispense under 80% N 2 + 20% CO 2 . Add components, except sodium crotonate solution, seven vitamin solution, sodium dithionite solution, NaHCO 3 solution, and Na 2 S·9H 2 O solution, to distilled/deionized water and bring volume to 910.0mL. Mix thoroughly. Sparge with 80% N 2 + 20% CO 2 . Auto- clave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobi- cally add 50.0mL of sterile sodium crotonate solution, 20.0mL of sterile NaHCO 3 solution, 10.0mL of seven vitamin solution, and 10.0mL of sterile Na 2 S·9H 2 O solution. Mix thoroughly. Aseptically and anaerobically distribute into sterile tubes or flasks. After inocula- tion, add 0.1mL of sodium dithionite solution per 10.0mL of medium. Use: For the cultivation of Syntrophobacter buswellii. SB/SW Medium Composition per liter: NaCl 1.0g KCl 0.5g MgCl 2 ·6H 2 O 0.4g NH 4 Cl 0.25g KH 2 PO 4 0.2g CaCl 2 ·2H 2 O 0.15g © 2010 by Taylor and Francis Group, LLC 1542 SC Agar Resazurin 1.0mg Sodium pyruvate solution 50.0mL NaHCO 3 solution 20.0mL Na 2 S·9H 2 O solution 10.0mL Seven vitamin solution 10.0mL Sodium dithionite solution 10.0mL Trace elements solution SL-10 1.0mL pH 7.2 ± 0.2 at 25°C Trace Elements Solution SL-10: Composition per liter: FeCl 2 ·4H 2 O 1.5g CoCl 2 ·6H 2 O 190.0mg MnCl 2 ·4H 2 O 100.0mg ZnCl 2 70.0mg Na 2 MoO 4 ·2H 2 O 36.0mg NiCl 2 ·6H 2 O 24.0mg H 3 BO 3 6.0mg CuCl 2 ·2H 2 O 2.0mg HCl (25% solution) 10.0mL Preparation of Trace Elements Solution SL-10: Prepare and dis- pense under 80% N 2 + 20% CO 2 . Add FeCl 2 ·4H 2 O to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring vol- ume to 1.0L. Add remaining components. Mix thoroughly. Sparge with 80% N 2 + 20% CO 2 . Autoclave for 15 min at 15 psi pressure–121°C. Sodium Pyruvate Solution: Composition per 50.0mL: Sodium pyruvate 1.25g Preparation of Sodium Pyruvate Solution: Add sodium pyru- vate to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize. Sparge with 80% N 2 + 20% CO 2 . NaHCO 3 Solution: Composition per 20.0mL: NaHCO 3 2.5g Preparation of NaHCO 3 Solution: Add NaHCO 3 to distilled/de- ionized water and bring volume to 20.0mL. Mix thoroughly. Filter ster- ilize. Sparge with 80% N 2 + 20% CO 2 . Na 2 S·9H 2 O Solution: Composition per 10.0mL: Na 2 S·9H 2 O 0.36g Preparation of Na 2 S·9H 2 O Solution: Add Na 2 S·9H 2 O to dis- tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N 2 . Autoclave for 15 min at 15 psi pressure–121°C. Seven Vitamin Solution: Composition per liter: Pyridoxine·HCl 0.3g Thiamine·HCl 0.2g Nicotinic acid 0.2g Calcium DL-pantothenate 0.1g Vitamin B 12 0.1g p-Aminobenzoic acid 80.0mg Biotin 20.0mg Preparation of Seven Vitamin Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sodium Dithionite Solution: Composition per 10.0mL: Sodium dithioninium 0.2g Preparation of Sodium Dithionite Solution: Add sodium dithi- oninium to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N 2 . Autoclave for 15 min at 15 psi pres- sure–121°C. Preparation of Medium: Prepare medium and dispense under 80% N 2 + 20% CO 2 . Add components, except sodium pyruvate solution, seven vitamin solution, sodium dithionite solution, NaHCO 3 solution, and Na 2 S·9H 2 O solution, to distilled/deionized water and bring volume to 910.0mL. Mix thoroughly. Sparge with 80% N 2 + 20% CO 2 . Auto- clave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobi- cally add 50.0mL of sterile sodium pyruvate solution, 20.0mL of sterile NaHCO 3 solution, 10.0mL of seven vitamin solution, and 10.0mL of sterile Na 2 S·9H 2 O solution. Mix thoroughly. Aseptically and anaerobi- cally distribute into sterile tubes or flasks. After inoculation, add 0.1mL of sodium dithionite solution per 10.0mL of medium. Use: For the cultivation of Syntrophobacter wolinii. SC Agar (DSMZ Medium 751) Composition per liter: Agar 15.0g Peptone from soy meal 8.0g Corn meal, solids from infusion 2.0g K 2 HPO 4 1.0g KH 2 PO 4 1.0g MgSO 4 ·7H 2 O 0.2g Hemin solution 15.0mL Serum albumin solution 10.0mL Cysteine solution 10.0mL Glucose solution 5.0mL pH 6.6 ± 0.2 at 25°C Hemin Solution: Composition per 100.0mL: Hemin 0.1g Preparation of Hemin Solution: Add hemin to 100.0mL 0.05N NaOH. Mix thoroughly. Serum Albumin Solution: Composition per 10.0mL: Bovine serum albumin, fraction V 2.0g Preparation of Serum Albumin Solution: Add bovine serum al- bumin, fraction V to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Glucose Solution: Composition per 10.0mL: D-Glucose 1.0g Preparation of Glucose Solution: Add glucose to distilled/deion- ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril- ize. Cysteine Solution: Composition per 10.0mL: L-Cysteine·HCl·H 2 O 1.0g Preparation of Cysteine Solution: Add L-cysteine·HCl·H 2 O to distilled/deionized water and bring volume to 10.0mL. Mix thorough- ly. Filter sterilize. Preparation of Medium: Add components, except glucose solu- tion, serum albumin solution, and cysteine solution, to distilled/deion- ized water and bring volume to 975.0mL. Mix thoroughly. Gently heat © 2010 by Taylor and Francis Group, LLC SC Agar 1543 and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 10.0mL sterile cysteine solution, 10.0mL sterile glucose solution, and 10.0mL sterile serum albumin so- lution. Mix thoroughly. Adjust pH to 6.6. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation and maintenance of Leifsonia xyli subsp. cyno- dontis=Clavibacter xyli subsp. cynodonti. SC Agar Composition per liter: Agar 15.0g Papaic digest of soybean meal 8.0g Corn meal (solids from infusion) 2.0g K 2 HPO 4 1.0g KH 2 PO 4 1.0g MgSO 4 ·7H 2 O 0.2g Hemin solution 15.0mL Bovine serum albumin, fraction V solution 10.0mL L-Cysteine·H 2 O solution 10.0mL Glucose solution 1.0mL pH 6.6 ± 0.2 at 25°C Hemin Solution: Composition per 100.0mL: Hemin 0.1g NaOH (0.05N solution) 100.0mL Preparation of Hemin Solution: Add hemin to NaOH solution. Mix thoroughly. Bovine Serum Albumin, Fraction V Solution: Composition per 10.0mL: Bovine serum albumin, fraction V 2.0g Preparation of Bovine Serum Albumin, Fraction V Solu- tion: Add bovine serum albumin to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. L-Cysteine·H 2 O Solution: Composition per 10.0mL: L-Cysteine·H 2 O 1.0g Preparation of L-Cysteine·H 2 O Solution: Add L-cysteine·H 2 O to distilled/deionized water and bring volume to 10.0mL. Mix thor- oughly. Filter sterilize. Glucose Solution: Composition per 10.0mL: D-Glucose 5.0g Preparation of Glucose Solution: Add glucose to distilled/deion- ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril- ize. Preparation of Medium: Add components—except bovine serum albumin solution, L-cysteine·H 2 O solution, and glucose solution—to distilled/deionized water and bring volume to 979.0mL. Mix thorough- ly. Adjust pH to 6.6 with NaOH. Gently heat and bring to boiling. Au- toclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile bovine serum albumin solution, 10.0mL of sterile L-cysteine·H 2 O solution, and 1.0mL of sterile glu- cose solution. Mix thoroughly. Pour into sterile Petri dishes or distrib- ute into sterile tubes. Use: For the cultivation and maintenance of Clavibacter xyli. SC Agar Composition per liter: Agar 20.0g (NH 4 ) 2 SO 4 5.0g KH 2 PO 4 1.0g MgSO 4 ·7H 2 O 0.5g NaCl 0.1g CaCl 2 ·2H 2 O 0.1g Inositol 2.0mg KI 1.0mg H 3 BO 3 0.5mg ZnSO 4 ·7H 2 O 0.4mg MnSO 4 ·4H 2 O 0.4mg Thiamine·HCl 0.4mg Pyroxidine·HCl 0.4mg Niacin 0.4mg Calcium pantothenate 0.4mg p-Aminobenzoic acid 0.2mg Riboflavin 0.2mg FeCl 3 0.2mg Na 2 MoO 4 ·4H 2 O 0.2mg CuSO 4 ·5H 2 O 0.04mg Folic acid 2.0μg Biotin 2.0μg Glucose solution 50.0mL Synthetic stock solution 30.0mL Glucose Solution: Composition per 100.0mL: Glucose 40.0g Preparation of Glucose Solution: Add glucose to distilled/deion- ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster- ilize. Synthetic Stock Solution: Composition per 300.0mL: L-Isoleucine 5.25g L-Arginine 3.48g L-Aspartic acid 2.66g L-Leucine 2.62g L-Methionine 1.49g L-Threonine 1.19g L-Valine 1.17g L-Serine 1.05g L-Lysine 0.91g L-Phenylalanine 0.83g L-Tryptophan 0.82g L-Histidine 0.58g m-Inositol 0.36g Uracil 0.22g L-Tyrosine 0.18g Adenine 0.135g Preparation of Synthetic Stock Solution: Add components to distilled/deionized water and bring volume to 300.0mL. Mix thorough- ly. Filter sterilize. Store in the dark at 25°C. Preparation of Medium: Add components, except synthetic stock solution and glucose solution, to distilled/deionized water and bring volume to 920.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 30.0mL of sterile synthetic stock solution and 50.0mL of sterile glucose solution. Mix thoroughly. Pour into sterile Petri dish- es or distribute into sterile tubes. © 2010 by Taylor and Francis Group, LLC 1544 SC Agar without Histidine Use: For the cultivation and maintenance of Saccharomyces cerevi- siae. SC Agar without Histidine Composition per liter: Agar 20.0g (NH 4 ) 2 SO 4 5.0g KH 2 PO 4 1.0g MgSO 4 ·7H 2 O 0.5g NaCl 0.1g CaCl 2 ·2H 2 O 0.1g Inositol 2.0mg KI 1.0mg H 3 BO 3 0.5mg ZnSO 4 ·7H 2 O 0.4mg MnSO 4 ·4H 2 O 0.4mg Thiamine·HCl 0.4mg Pyroxidine·HCl 0.4mg Niacin 0.4mg Calcium pantothenate 0.4mg p-Aminobenzoic acid 0.2mg Riboflavin 0.2mg FeCl 3 0.2mg Na 2 MoO 4 ·4H 2 O 0.2mg CuSO 4 ·5H 2 O 0.04mg Folic acid 2.0μg Biotin 2.0μg Glucose solution 50.0mL Synthetic stock solution 30.0mL Glucose Solution: Composition per 100.0mL: Glucose 40.0g Preparation of Glucose Solution: Add glucose to distilled/deion- ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster- ilize. Synthetic Stock Solution: Composition per 300.0mL: L-Isoleucine 5.25g L-Arginine 3.48g L-Aspartic acid 2.66g L-Leucine 2.62g L-Methionine 1.49g L-Threonine 1.19g L-Valine 1.17g L-Serine 1.05g L-Lysine 0.91g L-Phenylalanine 0.83g L-Tryptophan 0.82g Myo-inositol 0.36g Uracil 0.22g L-Tyrosine 0.18g Adenine 0.135g Preparation of Synthetic Stock Solution: Add components to distilled/deionized water and bring volume to 300.0mL. Mix thorough- ly. Filter sterilize. Store in the dark at 25°C. Preparation of Medium: Add components, except synthetic stock solution and glucose solution, to distilled/deionized water and bring volume to 920.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 30.0mL of sterile synthetic stock solution and 50.0mL of sterile glucose solution. Mix thoroughly. Pour into sterile Petri dish- es or distribute into sterile tubes. Use: For the cultivation and maintenance of strains of Saccharomyces cerevisiae that do not require histidine. SC Agar without Leucine and Tryptophan Composition per liter: Agar 20.0g (NH 4 ) 2 SO 4 5.0g KH 2 PO 4 1.0g MgSO 4 ·7H 2 O 0.5g NaCl 0.1g CaCl 2 ·2H 2 O 0.1g Inositol 2.0mg KI 1.0mg H 3 BO 3 0.5mg ZnSO 4 ·7H 2 O 0.4mg MnSO 4 ·4H 2 O 0.4mg Thiamine·HCl 0.4mg Pyroxidine·HCl 0.4mg Niacin 0.4mg Calcium pantothenate 0.4mg p-Aminobenzoic acid 0.2mg Riboflavin 0.2mg FeCl 3 0.2mg Na 2 MoO 4 ·4H 2 O 0.2mg CuSO 4 ·5H 2 O 0.04mg Folic acid 2.0μg Biotin 2.0μg Glucose solution 50.0mL Synthetic stock solution 30.0mL Glucose Solution: Composition per 100.0mL: Glucose 40.0g Preparation of Glucose Solution: Add glucose to distilled/deion- ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster- ilize. Synthetic Stock Solution: Composition per 300.0mL: L-Isoleucine 5.25g L-Arginine 3.48g L-Aspartic acid 2.66g L-Methionine 1.49g L-Threonine 1.19g L-Valine 1.17g L-Serine 1.05g Lysine 0.91g L-Phenylalanine 0.83g L-Histidine 0.58g Myo-inositol 0.36g Uracil 0.22g Adenine 0.135g L-Tyrosine 0.18g Preparation of Synthetic Stock Solution: Add components to distilled/deionized water and bring volume to 300.0mL. Mix thorough- ly. Filter sterilize. Store in the dark at 25°C. Preparation of Medium: Add components, except synthetic stock solution and glucose solution, to distilled/deionized water and bring © 2010 by Taylor and Francis Group, LLC . Aseptically and anaerobi- cally add 50.0mL of sterile sodium crotonate solution, 20.0mL of sterile NaHCO 3 solution, 10.0mL of seven vitamin solution, and 10.0mL of sterile Na 2 S·9H 2 O solution. Mix. Aseptically and anaerobi- cally add 50.0mL of sterile sodium pyruvate solution, 20.0mL of sterile NaHCO 3 solution, 10.0mL of seven vitamin solution, and 10.0mL of sterile Na 2 S·9H 2 O solution. Mix. tolerance of a variety of microorganisms. © 2010 by Taylor and Francis Group, LLC Saprospira grandis Medium 1539 Salt Tolerance Medium, Gilardi Composition per liter: NaCl 65.0g Pancreatic digest of