S Medium 1525 Ryan’s Aeromonas Medium See: Aeromonas Medium R2YE Medium Composition per 1062.2mL: Thiostrepton 50.0mg Basal solution 800.0mL TES (N-tris[hydroxymethyl]methyl-2-amino–ethane- sulfonic acid) buffer 100.0mL CaCl 2 ·2H 2 O solution 80.2mL Yeast extract solution 50.0mL L-Proline solution 15.0mL KH 2 PO 4 solution 10.0mL NaOH solution 5.0mL Trace elements solution 2.0mL pH 7.2 ± 0.2 at 25°C Basal Solution: Composition per 800.0mL: Sucrose 103.0g MgCl 2 ·6H 2 O 10.12g D-Glucose 10.0g K 2 SO 4 0.25g Casamino acids 0.1g Preparation of Basal Solution: Add components to distilled/de- ionized water and bring volume to 800.0mL. Mix thoroughly. Auto- clave for 15 min at 15 psi pressure–121°C. Cool to 25°C. TES Buffer: Composition per liter: TES (N-tris[hydroxymethyl] methyl-2-amino–ethane- sulfonic acid) buffer 57.3g Preparation of TES Buffer: Add TES to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Filter sterilize. CaCl 2 ·2H 2 O Solution: Composition per 100.0mL: CaCl 2 ·2H 2 O 3.68g Preparation of CaCl 2 ·2H 2 O Solution: Add the CaCl 2 ·2H 2 O to distilled/deionized water and bring volume to 100.0mL. Mix thorough- ly. Filter sterilize. Yeast Extract Solution: Composition per 100.0mL: Yeast extract 10.0g Preparation of Yeast Extract Solution: Add yeast extract to dis- tilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. L-Proline Solution: Composition per 100.0mL: L-Proline 20.0g Preparation of L-Proline Solution: Add the proline to distilled/ deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. KH 2 PO 4 Solution: Composition per 100.0mL: KH 2 PO 4 0.5g Preparation of KH 2 PO 4 Solution Solution: Add the KH 2 PO 4 to distilled/deionized water and bring volume to 100.0mL. Mix thorough- ly. Filter sterilize. NaOH Solution: Composition per 100.0mL: NaOH 40.0g Preparation of NaOH Solution: Add the NaOH to distilled/deion- ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster- ilize Trace Elements Solution: Composition per liter: FeCl 3 ·6H 2 O 0.2g ZnCl 2 0.04g CuCl 2 ·2H 2 O 0.01g MnCl 2 ·4H 2 O 0.01g Na 2 B 4 O 7 ·10H 2 O 0.01g (NH 4 ) 6 Mo 7 O 24 ·4H 2 O 0.01g Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Preparation of Medium: To 800.0mL of cooled, sterile basal solu- tion, aseptically add the remaining components. Mix thoroughly. Asep- tically distribute into sterile tubes or flasks. Use: For the cultivation and maintenance of Streptomyces lividans. S. aureus ID Composition per liter: Proprietary Source: This medium is available from bioMérieux. Use: For the direct identification of Staphylococcus aureus and the se- lective isolation of staphylococci. Direct identification of S. aureus is based on the spontaneous green coloration of α-glucosidase-producing colonies. S Broth Composition per liter: Peptone 10.0g Meat extract 2.4g NaCl 2.0g pH 7.0 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation of Bacillus cereus. S Medium Composition per liter: Glycogen 3.0g MgSO 4 ·7H 2 O 2.0g L-Leucine 1.0g L-Tyrosine 0.6g L-Asparagine 0.5g L-Isoleucine 0.5g L-Proline 0.5g L-Lysine 0.25g KH 2 PO 4 0.13g © 2010 by Taylor and Francis Group, LLC 1526 S Salts Djenkolic acid 0.1g L-Arginine 0.1g L-Serine 0.1g L-Threonine 0.1g L-Valine 0.1g L-Alanine 0.05g L-Glycine 0.05g L-Histidine 0.05g L-Methionine 0.05g L-Tryptophan 0.05g pH 7.6 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Asep- tically distribute into tubes or flasks. Use: For the cultivation of Myxococcus xanthus. S Salts Composition per liter: Dibenzothiophene 5.0g NH 4 Cl 0.5g KH 2 PO 4 0.25g MgCl 2 ·6H 2 O 0.25g pH 6.5–7.0 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.5–7.0 with KOH. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation of Bacillus sulfasportare. S6 Medium for Thiobacilli Composition per liter: Agar 15.0g Na 2 S 2 O 3 10.0g KH 2 PO 4 11.8g Na 2 HPO 4 1.2g MgSO 4 ·7H 2 O 0.1g (NH 4 ) 2 SO 4 0.1g CaCl 2 0.03g FeCl 3 0.02g MnSO 4 0.02g Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of Thiobacillus denitrificans and Thiobacillus thioparus. S8 Medium for Thiobacilli Composition per liter: Agar 15.0g KH 2 PO 4 11.8g Na 2 S 2 O 3 10.0g KNO 3 5.0g Na 2 HPO 4 1.2g NaHCO 3 0.5g MgSO 4 ·7H 2 O 0.1g (NH 4 ) 2 SO 4 0.1g CaCl 2 0.03g FeCl 3 0.02g MnSO 4 0.02g Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of Thiobacillus neapolita- nus. SA Agar Composition per liter: Agar 15.0g Pancreatic digest of casein 10.0g NaCl 5.0g Starch, soluble 1.0g Ampicillin 0.01g Phenol Red 0.018g pH 7.4 ± 0.2 at 25°C Preparation of Medium: Add components, except ampicillin, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres- sure–121°C. Cool to 45°–50°C. Aseptically add ampicillin. Mix thor- oughly. Pour into sterile Petri dishes. Use: For the isolation, cultivation, and differentiation, based on starch hydrolysis, of Aeromonas hydrophila from foods. After inoculation of plates and growth of cultures, starch hydrolysis is determined by flood- ing each plate with 5.0mL of Lugol’s iodine solution. SA Agar, Modified (Lachica’s Medium) Composition per liter: Beef heart, solids from infusion 500.0g Agar 15.0g Tryptose 10.0g NaCl 5.0g Amylose Azure 3.0g Ampicillin 0.01mg pH 7.4 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes. Use: For the isolation and cultivation of Aeromonas hydrophila from foods. Aeromonas hydrophila appears as colonies surrounded by a light halo on a light blue background. SA HiVeg Agar Base with Ampicillin Composition per liter: Agar 15.0g Plant hydrolysate 10.0g Starch, soluble 10.0g NaCl 5.0g Phenol Red 0.025g Ampicillin solution 10.0mL pH 7.4 ± 0.2 at 25°C Source: This medium, without ampicillin, is available as a premixed powder from HiMedia. © 2010 by Taylor and Francis Group, LLC SABHI Blood Agar 1527 Ampicillin Solution: Composition per 10.0mL: Ampicillin 0.01g Preparation of Ampicillin Solution: Add ampicillin to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except ampicillin solu- tion, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°C. Aseptically add sterile ampicil- lin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the isolation, cultivation, and differentiation of Aeromonas hydrophilia from foods based on starch hydrolysis. SABHI Agar (Sabouraud Glucose and Brain Heart Infusion Agar) Composition per liter: Glucose 21.0g Agar 15.0g Pancreatic digest of casein 10.5g Peptic digest of animal tissue 5.0g Brain heart, solids from infusion 4.0g NaCl 2.5g Na 2 HPO 4 1.25g pH 6.8 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes in 20.0mL volumes or leave in tubes. Use: For the cultivation of dermatophytes and other pathogenic and nonpathogenic fungi from clinical and nonclinical specimens. SABHI Agar Composition per liter: Beef heart, infusion from 125.0g Calf brains, infusion from 100.0g Glucose 21.0g Agar 15.0g Neopeptone 5.0g Proteose peptone 5.0g NaCl 2.5g Na 2 HPO 4 1.25g Chloromycetin solution 1.0mL pH 7.0 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Chloromycetin Solution: Composition per 10.0mL: Chloromycetin 1.0g Preparation of Chloromycetin Solution: Add chloromycetin to distilled/deionized water and bring volume to 10.0mL. Mix thorough- ly. Filter sterilize. Preparation of Medium: Add components, except chloromycetin solution, to distilled/deionized water and bring volume to 999.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 1.0mL of sterile chloromycetin solution. Mix thoroughly. Aseptically distribute into sterile tubes in 5.0mL volumes. Use: For the cultivation of dermatophytes and other pathogenic and nonpathogenic fungi from clinical and nonclinical specimens. SABHI Agar, Modified Composition per liter: Beef heart, infusion from 62.5g Calf brain, infusion from 50.0g Glucose 20.5g Brain heart infusion broth 18.6g Agar 7.5g Neopeptone 5.0g Pancreatic digest of gelatin 2.5g NaCl 1.25g Na 2 HPO 4 0.625g pH 6.8 ± 0.2 at 25°C Preparation of Medium: Dissolve, then autoclave at 121°C for 15 min. Cool to 50°C and add 1.0mL of sterile chloramphenicol solution (100.0mg/mL). Mix well and dispense into sterile tubes. Slant and al- low to harden. Refrigerate until needed. Use: For the cultivation of dermatophytes and other pathogenic and nonpathogenic fungi from clinical and nonclinical specimens. SABHI Blood Agar Composition per liter: Beef heart, infusion from 125.0g Calf brains, infusion from 100.0g Glucose 21.0g Agar 15.0g Neopeptone 5.0g Proteose peptone 5.0g NaCl 2.5g Na 2 HPO 4 1.25g Blood 100.0mL Chloromycetin solution 1.0mL pH 7.0 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Chloromycetin Solution: Composition per 10.0mL: Chloromycetin 1.0g Preparation of Chloromycetin Solution: Add chloromycetin to distilled/deionized water and bring volume to 10.0mL. Mix thorough- ly. Filter sterilize. Preparation of Medium: Add components, except blood and chloro- mycetin solution, to distilled/deionized water and bring volume to 899.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL of sterile blood and 1.0mL of sterile chloromycetin solution. Sheep blood or human blood may be used. Mix thoroughly. Aseptically distribute into sterile tubes in 5.0mL volumes. © 2010 by Taylor and Francis Group, LLC 1528 SABHI HiVeg Agar Base with Chloramphenicol Use: For the cultivation of dermatophytes and other pathogenic and nonpathogenic fungi from clinical and nonclinical specimens. Blood enhances the recovery of Blastomyces dermatitidis and Histoplasma capsulatum and their conversion to the yeast phase. SABHI HiVeg Agar Base with Chloramphenicol Composition per liter: Glucose 21.0g Agar 15.0g Plant infusion 5.14g Plant peptone No. 3 5.0g Plant special peptone 5.0g Plant special infusion 4.11g NaCl 2.5g Na 2 HPO 4 1.25g Chloramphenicol solution 10.0mL pH 7.0 ± 0.2 at 25°C Source: This medium, without chloramphenicol, is available as a pre- mixed powder from HiMedia. Chloramphenicol Solution: Composition per 10.0mL: Chloramphenicol 0.1g Preparation of Chloramphenicol Solution: Add chlorampheni- col to distilled/deionized water and bring volume to 10.0mL. Mix thor- oughly. Filter sterilize. Preparation of Medium: Add components, except chlorampheni- col solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile chloramphenicol solution. Mix thoroughly. Pour into Petri dishes or aseptically distribute into sterile tubes. Use: For the cultivation of dermatophytes and other pathogenic and nonpathogenic fungi from clinical and nonclinical specimens. SABHI HiVeg Agar Base with Chloromycetin Composition per liter: Glucose 21.0g Agar 15.0g Plant infusion 5.14g Plant peptone No. 3 5.0g Plant special peptone 5.0g Plant special infusion 4.11g NaCl 2.5g Na 2 HPO 4 1.25g Chloromycetin solution 1.0mL pH 7.0 ± 0.2 at 25°C Source: This medium, without chloromycetin, is available as a pre- mixed powder from HiMedia. Chloromycetin Solution: Composition per 10.0mL: Chloromycetin 1.0g Preparation of Chloromycetin Solution: Add chloromycetin to distilled/deionized water and bring volume to 10.0mL. Mix thorough- ly. Filter sterilize. Preparation of Medium: Add components, except chloromycetin solution, to distilled/deionized water and bring volume to 999.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 1.0mL of sterile chloromycetin solution. Mix thoroughly. Aseptically distribute into sterile tubes in 5.0mL volumes. Use: For the cultivation of dermatophytes and other pathogenic and nonpathogenic fungi from clinical and nonclinical specimens. SABHI HiVeg Agar Base with Blood and Chloromycetin Composition per liter: Glucose 21.0g Agar 15.0g Plant infusion 5.14g Plant peptone No. 3 5.0g Plant special peptone 5.0g Plant special infusion 4.11g NaCl 2.5g Na 2 HPO 4 1.25g Blood 100.0mL Chloromycetin solution 1.0mL pH 7.0 ± 0.2 at 25°C Source: This medium, without blood and chloromycetin, is available as a premixed powder from HiMedia. Chloromycetin Solution: Composition per 10.0mL: Chloromycetin 1.0g Preparation of Chloromycetin Solution: Add chloromycetin to distilled/deionized water and bring volume to 10.0mL. Mix thorough- ly. Filter sterilize. Preparation of Medium: Add components, except blood and chloro- mycetin solution, to distilled/deionized water and bring volume to 899.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL of sterile blood and 1.0mL of sterile chloromycetin solution. Sheep blood or human blood may be used. Mix thoroughly. Aseptically distribute into sterile tubes in 5.0mL volumes. Use: For the cultivation of dermatophytes and other pathogenic and nonpathogenic fungi from clinical and nonclinical specimens. Blood enhances the recovery of Blastomyces dermatitidis and Histoplasma capsulatum and their conversion to the yeast phase. Sabouraud Agar Composition per liter: Neopeptone 30.0g Agar 20.0g pH 6.8 ± 0.2 at 25°C Preparation of Medium: Add components to tap water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Dis- tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation of yeasts and molds. Sabouraud Agar with CCG and 3% Sodium Chloride Composition per 3031.5mL: Glucose 120.0g NaCl 90.0g Agar 45.0g Peptone 30.0g Chloramphenicol solution 15.0mL © 2010 by Taylor and Francis Group, LLC Sabouraud Agar, Modified 1529 Cycloheximide solution 15.0mL Gentamicin solution 1.5mL Chloramphenicol Solution: Composition per 15.0mL: Chloramphenicol 0.15g Preparation of Chloramphenicol Solution: Add chlorampheni- col to distilled/deionized water and bring volume to 15.0mL. Mix thor- oughly. Filter sterilize. Cycloheximide Solution: Composition per 15.0mL: Cycloheximide 0.3g Preparation of Cycloheximide Solution: Add cycloheximide to distilled/deionized water and bring volume to 15.0mL. Mix thorough- ly. Filter sterilize. Caution: Cycloheximide is toxic. Avoid skin contact or aerosol for- mation and inhalation. Gentamicin Solution: Composition per 10.0mL: Gentamicin 0.4g Preparation of Gentamicin Solution: Add gentamicin to dis- tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components—except chloramphen- icol solution, cycloheximide solution, and gentamicin solution—to dis- tilled/deionized water and bring volume to 3.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres- sure–121°C. Cool to 45°–50°C. Aseptically add 15.0mL of sterile chloramphenicol solution, 15.0mL of sterile cycloheximide solution, and 1.5mL of sterile gentamicin solution. Mix thoroughly. Aseptically distribute into sterile tubes. Allow tubes to cool in a slanted position. Use: For the selective isolation and cultivation of fungi from speci- mens with a mixed flora. Sabouraud Agar with CCG and 5% Sodium Chloride Composition per 3031.5mL: NaCl 150.0g Glucose 120.0g Agar 45.0g Peptone 30.0g Chloramphenicol solution 15.0mL Cycloheximide solution 15.0mL Gentamicin solution 1.5mL Chloramphenicol Solution: Composition per 15.0mL: Chloramphenicol 0.15g Preparation of Chloramphenicol Solution: Add chlorampheni- col to distilled/deionized water and bring volume to 15.0mL. Mix thor- oughly. Filter sterilize. Cycloheximide Solution: Composition per 15.0mL: Cycloheximide 0.3g Preparation of Cycloheximide Solution: Add cycloheximide to distilled/deionized water and bring volume to 15.0mL. Mix thorough- ly. Filter sterilize. Caution: Cycloheximide is toxic. Avoid skin contact or aerosol for- mation and inhalation. Gentamicin Solution: Composition per 10.0mL: Gentamicin 0.4g Preparation of Gentamicin Solution: Add gentamicin to dis- tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components—except chloramphen- icol solution, cycloheximide solution, and gentamicin solution—to dis- tilled/deionized water and bring volume to 3.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres- sure–121°C. Cool to 45°–50°C. Aseptically add 15.0mL of sterile chloramphenicol solution, 15.0mL of sterile cycloheximide solution, and 1.5mL of sterile gentamicin solution. Mix thoroughly. Aseptically distribute into sterile tubes. Allow tubes to cool in a slanted position. Use: For the selective isolation and cultivation of fungi from speci- mens with a mixed flora. Sabouraud Agar, Diluted 1/10 Composition per liter: Agar 20.0g Glucose 4.0g KH 2 PO 4 1.5g MgSO 4 ·7H 2 O 1.0g NaNO 3 1.0g Peptone 1.0g pH 6.8 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.8. Gently heat and bring to boiling. Distribute into tubes or flasks. Auto- clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of a variety of fungi and het- erotrophic bacteria. Sabouraud Agar, Diluted 1/10 with Salt Composition per liter: Agar 15.0g Glucose 2.0g KH 2 PO 4 1.0g MgSO 4 ·7H 2 O 1.0g Mycological peptone 1.0g pH 6.8–7.0 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.8–7.0. Gently heat and bring to boiling. Distribute into tubes or flasks. Auto- clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of Arthroderma benhamiae, Arthroderma vanbreuseghemii, Microsporum canis, and Trichophyton mentagrophytes. Sabouraud Agar, Modified Composition per liter: Agar 20.0g Glucose 20.0g Neopeptone 10.0g pH 7.0 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC 1530 Sabouraud Chloramphenicol HiVeg Agar Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation of yeasts, molds, and aciduric bacteria. Sabouraud Chloramphenicol HiVeg Agar Composition per liter: Glucose 40.0g Agar 15.0g Plant hydrolysate 5.0g Plant peptone 5.0g Chloramphenicol 0.05g Chloramphenicol solution 10.0mL pH 5.6 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and identification of yeasts. Sabouraud Cycloheximide Chloramphenicol HiVeg Agar Composition per liter: Glucose 20.0g Agar 15.0g Plant peptone 10.0g Cycloheximide 0.5g Chloramphenicol 0.04g pH 6.8 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Caution: Cycloheximide is very toxic. Avoid skin contact or aerosol formation and inhalation. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the isolation and cultivation of pathogenic fungi. Sabouraud Dextrose Agar (BAM M133) Composition per liter: Neopeptone 30.0g Agar 20.0g pH 5.6 ± 0.2 at 25°C Preparation of Medium: Add components to tap water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Dis- tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation of yeasts and molds. Sabouraud Dextrose Agar pH 5.6 Composition per liter: Glucose 40.0g Agar 15.0g Mycological peptone 10.0 g pH 5.6 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Oxoid Unipath. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 50°C. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation of yeasts and other fungi. For use in qualita- tive and quantitative procedures for yeasts and fungi. Sabouraud Glucose Agar (Saboraud Dextrose Agar) (SabDex, 2%) Composition per liter: Glucose 20.0g Agar 15.0g Pancreatic digest of casein 5.0g Peptic digest of animal tissue 5.0g pH 5.6 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation of yeast and filamentous fungi. For the culti- vation of pathogenic and nonpathogenic fungi, especially dermato- phytes. The medium may be made more selective for fungi by the addi- tion of chloramphenicol. Fluconozole (final concentration of 8–16mg per mL) may also be added to test for antibiotic sensitivity. Sabouraud Glucose Agar (Saboraud Dextrose Agar) (SabDex, 4%) Composition per liter: Glucose 40.0g Agar 15.0g Pancreatic digest of casein 5.0g Peptic digest of animal tissue 5.0g pH 5.6 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems and Oxoid Unipath. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation of yeast and filamentous fungi. For the culti- vation of pathogenic and nonpathogenic fungi, especially dermato- phytes. The medium may be made more selective for fungi by the addi- tion of chloramphenicol. Fluconozole (final concentration of 8–16mg per mL) may also be added to test for antibiotic sensitivity. © 2010 by Taylor and Francis Group, LLC Sabouraud Glucose Broth 1531 Sabouraud Glucose Agar, Emmons Composition per liter: Glucose 20.0g Agar 17.0g Pancreatic digest of casein 5.0g Peptic digest of animal tissue 5.0g pH 6.9 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 13 psi pressure–118°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation of dermatophytes and other pathogenic and nonpathogenic fungi from clinical and nonclinical specimens. For the cultivation of yeast and filamentous fungi. Sabouraud Glucose Agar with Chloramphenicol and Cycloheximide Composition per liter: Glucose 40.0g Agar 15.0g Pancreatic digest of casein 5.0g Peptic digest of animal tissue 5.0g Cycloheximide solution 10.0mL Chloramphenicol solution 10.0mL pH 5.6 ± 0.2 at 25°C Cycloheximide Solution: Composition per 10.0mL: Cycloheximide 0.5g Acetone 10.0mL Preparation of Cycloheximide Solution: Add cycloheximide to acetone. Mix thoroughly. Caution: Cycloheximide is toxic. Avoid skin contact or aerosol for- mation and inhalation. Chloramphenicol Solution: Composition per 10.0mL: Chloramphenicol 0.05g Ethanol (95% solution) 10.0mL Preparation of Chloramphenicol Solution: Add chlorampheni- col to ethanol. Mix thoroughly. Preparation of Medium: Add components, except cycloheximide solution and chloramphenicol solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Add the cycloheximide solution and chloramphenicol solu- tion. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and identification of yeasts. Sabouraud Glucose Agar, HiVeg Composition per liter: Glucose 40.0g Agar 15.0g Plant peptone No. 4 10.0g Selective supplement 10.0mL pH 6.9 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Selective Supplement: Composition per 10.0mL: Cycloheximide 0.5g Chloramphenicol 0.04g Preparation of Selective Supplement: Add components to dis- tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Caution: Cycloheximide is very toxic. Avoid skin contact or aerosol formation and inhalation. Preparation of Medium: Add components, except selective sup- plement, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 10.0mL sterile selective supplement. Mix thor- oughly. Pour into sterile Petri dishes or aseptically distribute into tubes. Use: For the cultivation of dermatophytes and other pathogenic and nonpathogenic fungi from clinical and nonclinical specimens. For the cultivation of yeast and filamentous fungi. Sabouraud Glucose Agar with Olive Oil Composition per liter: Glucose 40.0g Agar 15.0g Pancreatic digest of casein 5.0g Peptic digest of animal tissue 5.0g Olive oil 20.0mL Tween™ 80 2.0mL pH 5.6 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Allow tubes to cool in a slanted position. Use: For the cultivation and maintenance of Malassezia species. Sabouraud Glucose and Brain Heart Infusion Agar See: SABHI Agar Sabouraud Glucose Broth Composition per liter: Glucose 20.0g Neopeptone 10.0g pH 5.6 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Avoid over- heating. Use: For the cultivation of pathogenic and nonpathogenic fungi, espe- cially dermatophytes. The medium may be made more selective for fungi by the addition of chloramphenicol. © 2010 by Taylor and Francis Group, LLC 1532 Sabouraud Glucose HiVeg Agar Base, Modified with Cycloheximide and Chloramphenicol Sabouraud Glucose HiVeg Agar Base, Modified with Cycloheximide and Chloramphenicol (Glucose HiVeg Agar Base, Emmons) Composition per liter: Glucose 20.0g Agar 17.0g Plant special peptone 10.0g pH 7.0 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation of fungi. Sabouraud Glucose HiVeg Broth (Sabouraud Liquid HiVeg Medium) Composition per liter: Glucose 20.0g Plant special peptone 10.0g pH 5.6 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation of pathogenic and nonpathogenic fungi, espe- cially dermatophytes. The medium may be made more selective for fungi by the addition of chloramphenicol. Sabouraud Glucose Maltose HiVeg Agar Composition per liter: Agar 15.0g Glucose 10.0g Maltose 10.0g Plant hydrolysate 5.0g Plant peptone 5.0g pH 5.6 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Avoid overheating. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of a variety of fungi. Sabouraud Liquid Broth, Modified See: Antibiotic Medium 13 Sabouraud Maltose Agar Composition per liter: Maltose 40.0g Agar 15.0g Pancreatic digest of casein 5.0g Peptic digest of animal tissue 5.0g pH 5.6 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems and Oxoid Unipath. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Avoid overheating. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of a variety of fungi. Sabouraud Maltose Broth Composition per liter: Maltose 40.0g Neopeptone 10.0g pH 5.6 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Avoid over- heating. Use: For the cultivation of a variety of fungi. Sabouraud Maltose HiVeg Broth Composition per liter: Maltose 40.0g Plant peptone No. 4 10.0g pH 5.6 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Avoid overheating. Use: For the cultivation and maintenance of a variety of fungi. Sabouraud Medium, Emmons Modification Composition per liter: Glucose 20.0g Agar 15.0g Peptone 10.0g pH 6.8–7.0 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.8–7.0. Gently heat and bring to boiling. Distribute into tubes or flasks. Auto- clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of Arthroderma benhamiae, Arthroderma vanbreuseghemii, Aureobasidium pullulans, Epidermo- phyton floccosum, Microsporum canis, Sporothrix schenckii, Tricho- phyton mentagrophytes, and Trichophyton rubrum. © 2010 by Taylor and Francis Group, LLC Saccharolytic Clostridia Medium 1533 Sabouraud Medium, Fluid Composition per liter: Glucose 20.0g Pancreatic digest of casein 5.0g Peptamin 5.0g pH 5.7 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems and Oxoid Unipath. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Avoid over- heating. Use: For the isolation and cultivation of yeasts and molds. Sabouraud Medium, Fluid, HiVeg (Fluid Sabouraud HiVeg Medium) Composition per liter: Glucose 20.0g Plant hydrolysate 5.0g Plant peptone 5.0g pH 5.7 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation and maintenance of a variety of fungi. For the sterility testing of pharmaceutical preparations for the presence of molds and bacteria. Saccharococcus Agar Composition per liter: Agar 20.0g Beef extract 5.0g Sucrose 5.0g Pancreatic digest of casein 3.0g Glucose 1.0g pH 6.8 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of Saccharococcus thermo- philus. Saccharolytic Clostridia Medium Composition per liter: Pancreatic digest of casein 10.0g Yeast extract 6.0g Sodium thioglycolate 0.5g Carbohydrate solution 100.0mL Potassium phosphate (1M solution, pH 7.5) 30.0mL MgSO 4 (1M solution) 1.0mL Solution M 0.5mL FeSO 4 solution 0.2mL pH 7.0–7.2 at 25°C Carbohydrate Solution: Composition per 100.0mL: Glucose or sucrose 20.0g Preparation of Carbohydrate Solution: Add carbohydrate to distilled/deionized water and bring volume to 100.0mL. Mix thorough- ly. Filter sterilize. Solution M: Composition per liter: CaCl 2 3.33g MnCl 2 ·4H 2 O 1.98g Na 2 MoO 4 ·2H 2 O 1.21g CoCl 2 ·6H 2 O 1.19g Preparation of Solution M: Add components to distilled/deion- ized water and bring volume to 1.0L. Mix thoroughly. FeSO 4 ·7H 2 O Solution: Composition per 10.0mL: FeSO 4 ·7H 2 O 55.6g H 2 SO 4 (0.1M solution) 10.0mL Preparation of FeSO 4 ·7H 2 O Solution: Add the FeSO 4 ·7H 2 O to 10.0mL of H 2 SO 4 solution. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except carbohydrate solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 20 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile carbohydrate solution. Mix thoroughly. Aseptically distribute into ster- ile tubes. Use: For the cultivation of saccharolytic Clostridium species. Saccharolytic Clostridia Medium Composition per liter: Sodium thioglycolate 1.0g K 2 HPO 4 0.8g KH 2 PO 4 0.2g MgSO 4 ·7H 2 O 0.2g NaCl 0.2g Na 2 MoO 4 ·2H 2 O 0.025g Yeast extract 0.01g FeSO 4 ·7H 2 O 0.01g MnSO 4 ·4H 2 O 0.01g CaCl2 0.01g Carbohydrate solution 100.0mL Soil extract 10.0mL Trace elements solution 1.0mL pH 7.2 ± 0.2 at 25°C Carbohydrate Solution: Composition per 100.0mL: Glucose or sucrose 10.0g Preparation of Carbohydrate Solution: Add glucose or sucrose to distilled/deionized water and bring volume to 100.0mL. Mix thor- oughly. Filter sterilize. Soil Extract: Composition per 200.0mL: Garden soil, neutral 100.0g Preparation of Soil Extract: Add garden soil to 100.0mL of tap water. Gently heat and bring to 130°C for 60 min. Cool to 45°C. Filter through Whatman #1 filter paper. Autoclave for 15 min at 15 psi pres- sure–121°C. Cool to 45°–50°C. © 2010 by Taylor and Francis Group, LLC 1534 Saccharomyces Medium Trace Elements Solution: Composition per liter: Na 2 B 4 O 7 ·10H 2 O 0.05g CoNO 3 ·6H 2 O 0.05g CdSO 4 ·2H 2 O 0.05g CuSO 4 ·5H 2 O 0.05g ZnSO 4 ·7H 2 O 0.05g MnSO 4 ·H 2 O 0.05g Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Medium: Add components, except sodium thiogly- colate and carbohydrate solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Add sodium thioglycolate. Mix thoroughly. Distribute 9.5mL into test tubes that contain inverted Durham tubes. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 0.5mL of ster- ile carbohydrate solution to each tube. Mix thoroughly. Use: For the isolation of N 2 -fixing, saccharolytic Clostridium species. Saccharomyces Medium Composition per liter: Glucose 20.0g Peptone 20.0g Agar 15.0g Yeast extract 10.0g Yeast nitrogen base without amino acids 6.7g (NH 4 ) 2 SO 4 5.0g Proline 4.0mg Preparation of Medium: Add glucose, yeast nitrogen base without amino acids, and proline to distilled/deionized water and bring volume to 200.0mL. Mix thoroughly. Filter sterilize. Add peptone, yeast ex- tract, (NH 4 ) 2 SO 4 , and agar to distilled/deionized water and bring vol- ume to 800.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Asepti- cally combine the two sterile solutions. Mix thoroughly. Pour into ster- ile Petri dishes or aseptically distribute into sterile tubes. Use: For the cultivation and maintenance of Saccharomyces cerevi- siae. Saccharomyces rouxii Medium Composition per liter: Glucose 400.0g Agar 20.0g Peptone 20.0g Yeast extract 10.0g Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation of Zygosaccharomyces rouxii. Use: For the cultivation of Monodictys austrina. Sakazakii DHL Agar Composition per liter: Agar 15.0g Casein enzymic hydrolysate 10.0g Meat peptone 10.0g Lactose 10.0g Sucrose 10.0g Meat extract 3.0g Na 2 S 2 O 3 2.0g Sodium deoxycholate 1.5g Sodium citrate 1.0g Ammonium iron (III) citrate 1.0g L-Cysteine·HCl·H 2 O 0.2g Neutral Red 0.03g pH 7.2 ± 0.2 at 25°C Source: This medium is available from HiMedia. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the detection and isolation of pathogenic Enterobacteriaceae from all types of specimens. Saline Czapek Agar (SCZA) Composition per liter: Sucrose 30.0g NaCl 25.0g Agar 15.0g NaNO 3 3.0g K 2 HPO 4 1.0g KCl 0.5g MgSO 4 ·7H 2 0 0.5g FeSO 4 ·7H 2 O 0.01g pH 7.3 ± 0.2 at 25°C Preparation of Medium: Add components, except sucrose, to dis- tilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Distribute into tubes or flasks. In a separate flask, add sucrose to dis- tilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave both solutions separately for 15 min at 15 psi pressure– 121°C. Cool to 50°C. Combine the sterile solutions. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Salinibacter ruber Agar (DSMZ Medium 936) Composition per liter: NaCl 195.0g MgSO 4 ·7H 2 O 49.5g MgCl 2 ·6H 2 O 34.6g Agar 20.0g KCl 5.0g CaCl 2 ·2H 2 O 1.25g Yeast extract 1.0g NaBr 0.625g NaHCO 3 0.25g pH 7.2 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of Salinibacter ruber. © 2010 by Taylor and Francis Group, LLC . starch hydrolysis, of Aeromonas hydrophila from foods. After inoculation of plates and growth of cultures, starch hydrolysis is determined by flood- ing each plate with 5.0mL of Lugol’s iodine. pressure–121°C. Use: For the cultivation and maintenance of a variety of fungi. For the sterility testing of pharmaceutical preparations for the presence of molds and bacteria. Saccharococcus Agar Composition. 0.01g Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Preparation of Medium: To 800.0mL of cooled, sterile