Cửa sổ Alignment Editor trong chương trình Mega...7Hình 2.13.. Cửa sổ DataType for Alignment trong chương trình Mega...7Hình 2.14.. Copy các đoạn trình tự vào chương trình Mega...8Hình 2
PHƯƠNG PHÁP NGHIÊN CỨU
Vật liệu
Ngân hàng dữ liệu genbank (NCBI)
Chương trình giải trình tự Chromas, chương trình xây dựng cây di truyền loàiMega.
Các bước thực hiện
Bước 1: Truy cập vào trang web NCBI (https://www.ncbi.nlm.nih.gov/)
Mở list All Databases chọn Nucleotide, Nhập Accession Number + 16S rRNA (Ví dụ: “KP699121.1 16S rRNA”) Nhấp Search.
Hình 2.1 Truy cập vào trang web NCBI.
Bước 2: Xác định sequence từ Accession Number của một phần trình tự gen 16S rRNA: Nhấp FASTA
Hình 2.2 Xác định sequence từ Accession number.
2.2.2 Blast và xác định tên loài của mẫu 1
Bước 1: Lưu file mẫu về thiết bị.
Bước 2: Tải Chromas (http://technelysium.com.au/wp/chromas/) và khởi động phần mềm.
Hình 2.4 Giao diện chương trình Chromas.
Bước 3: Chọn Open Chọn mẫu 1 Open.
Hình 2.5 Mẫu 1 trên chương trình Chromas.
Bước 4: Chọn Edit Copy Sequence Plain Text.
Hình 2.6 Copy trình tự từ Chromas.
Bước 5: Mở Word Paste trình tự mới copy từ Chromas.
Hình 2.7 Trình tự mẫu 1 trong Word.
Bước 6: Truy cập trang BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi) và nhấp vào Nucleotide BLAST.
Hình 2.8 Giao diện trang BLAST trên web NCBI.
Bước 7: Copy trình tự ở Word (Bước 4) vào khung sequence BLAST.
Hình 2.9 Copy trình tự vào trang BLAST trên web NCBI.
2.2.3 Xây dựng cây di truyền các accession number
Bước 1: Mở chương trình Mega.
Bước 2: Chọn “Align” sau đó chọn “Edit/Build Alignment”.
Hình 2.11 Giao diện chương trình Mega.
Bước 3: Cửa sổ “Alignment Editer” hiện ra, chọn “Create a new alignmet”, sau đó nhấn “OK”.
Hình 2.12 Cửa sổ Alignment Editor trong chương trình Mega.
Bước 4: Một cửa sổ hiện câu hỏi “Are you building a DNA or protein sequence alignment?”, chọn “DNA”.
Hình 2.13 Cửa sổ DataType for Alignment trong chương trình Mega.
Bước 5: Giao diện mới hiện ra Sao chép các trình tự 16s rRNA của mẫu 1 vào chương trình.
Hình 2.14 Copy các đoạn trình tự vào chương trình Mega.
Bước 6: Ctrl+A để chọn tất cả các trình tự Chọn “Alignment”, sau đó chọn
Hình 2.15 Chọn Align by MUSCLE.
Bước 7: Cửa sổ mới hiện ra chọn “Neighbor Joining” trong “Cluster Method” và nhấn “OK”
Hình 2.16 Cửa sổ MUSCLE Alignment Options trong chương trình Mega.
Bước 8: Kết quả hiện ra chọn “Data” ► “Export Alignment” ► “Mega Format” Lưu file lại với đuôi “.MES
Bước 9: Sau khi lưu thì đóng trang và quay lại trang đầu tiên chọn “File” ►
“Open A File/Sesion”, dẫn đến file vừa lưu ở Bước 8
Hình 2.18 Mở file vừa lưu.
Bước 10: Tiếp theo chọn “Distance”, rồi chọn “Compute Overall Mean Distance…”
Hình 2.19 Chọn Compute Overall Mean Distance.
Bước 11: Giao diện “M11: Analysis Preferences” hiện ra, ở dòng
“Model/Method” chọn “Jukes-Cantor model” rồi nhấn “OK”
Hình 2.20 Cửa sổ M11: Analysis Preferences.
Bước 12: Kết quả hiện ra với d = 0,06 < 1,00 (mẫu 18 loài) và d=0,07KP699121.1 Bacillus subtilis strain GnzIII 16S ribosomal RNA gene, partial sequence
CTATACATGCAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGGTTGTTTGAACCGCATGGTTCAAACATAAAAGGTGGCTTTGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAGGAA
>HF562894.1 Bacillus tequilensis partial 16S rRNA gene, isolate S42
TGCAAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGA CGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGG GAAACCGGGGCTAATACCGGATGGTTGTTTGAACCGCATGGTTCAAACAT AAAAGGTGGCTTTGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCT AGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAG AGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGA GGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACG CCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAG AACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTACCTAACCAGAAA GCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGC GTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCT GATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAA CTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCG TAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAAC TGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGG TAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCT TAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCA AGACTGAAACTCAAAGGAATTGACGGGGCCCGCACAGCGTGGAGCATGTG TTTATTCGAGCACGCGAGACTTACAGGTCT
>KT273321.1 Bacillus pumilus strain AZ-1 16S ribosomal RNA gene, partial sequence
GGGAGCTTGCTCCCGGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGAGCTAATACCGGATAGTTCCTTGAACCGCATGGTTCAAGGATGAAAGACGGTTTCGGCTGTCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGGGGTAATGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTGCGAGAGTAACTGCTCGCACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGAAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAACCCTAGAAATAGGGCTTTCCCTTCGGGGACAGAGTGACAGGTGGTGCATGGTTGTCCTCAGCTCGGGCCGGGAAATGTTGGGTTAAGTCCCGCAACGAACGCAACCTTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCCGGAGGAAGGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGGCTGCAAGACCGCAAGGT
>KT216619.1 Bacillus vallismortis strain DD007 16S ribosomal RNA gene, partial sequence
TGCCTAATACATGCAAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGGTTGTTTGAACCGCATGGTTCAAACATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAG
TACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGC GGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTC TTGACATCCTCTGACAATCCTAGAGATAGGACGTCCCCTTCGGGGGCAGAG TGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTA AGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGG CACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGT CAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACA GAACAAAGGGCAGCGAAACCGCGAGGTTAAGCCAATCCCACAAATCTGTT CTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTA GTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACAC ACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAA CCTTTTAGGAGCCAGCCGCCGAAGGTGGGACA
>KR709243.1 Bacillus cereus strain UBT4 16S ribosomal RNA gene, partial sequence
CGGCCAGTAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACAGAAGGGAGCTTGCTCCCGGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGAGCTAATACCGGATAGTTCCTTGAACCGCATGGTTCAAGGATGAAAGACGGTTTCGGCTGTCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGGGGTAATGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAG
GGAAGAACAAGTGCGAGAGTAACTGCTCGCACCTTGACGGTACCTAACCA GAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGC AAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAA GTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGG GAAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAA TGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTG TAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACC CTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGC CCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTC GCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGA GCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACAT CCTCTGACAACCCTAGAGATAGGGCTTTCCCTTCGGGGACAGAGTGACAG GTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCC GCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCT AAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATC ATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACAGAACA AAGGGCTGCAAGACCGCAAGGTTTAGCCAATCCCATAAATCTGTTCTCAGT TCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATC GCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCC CGTCACACCACGAGAGTTTGCAACACCCGAAGTCGGTGAGGTAACCTTTAT GGAGCCAGCCGCCGAAGGTGGGGCAGATGATTGGGGTGAAGTCGTAACAA GGTATCCGGTCATATCTGTTTCCTG
>KP940383.1 Bacillus safensis strain CCH3X 16S ribosomal RNA gene, partial sequence
CGCGGGCTATACATGCAAGTCGAGCGGACAGAAGGGAGCTTGCTCCCGGA TGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACT GGGATAACTCCGGGAAACCGGAGCTAATACCGGATAGTTCCTTGAACCGC ATGGTTCAAGGATGAAAGACGGTTTCGGCTGTCACTTACAGATGGACCCG CGGCGCATTAGCTAGTTGGTGGGGTAATGGCTCACCAAGGCGACGATGCG TAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCA GACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTC TGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTC TGTTGTTAGGGAAGAACAAGTGCGAGAGTAACTGCTCGCACCTTGACGGT ACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATAC GTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGC GGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATT GGAAACTGGGAAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTA GCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTC TCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGA TTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGG GGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGG AGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAA GCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGG TCTTGACATCCTCTGACAACCCTAGAGATAGGGCTTTCCCTTCGGGGACAG AGTGACAGGTGGTGCATGATGTCGTCAGCTCGTGTCGTGAGATGTTGGGTA GTCCCGCACGAGCGCACCTTGATCTAGTGCCAGCATTCAGTGGCACTCTAG TGACTGCATGACAACCGAGAAGGGGGGGAATGAACGTCAATCATC
>KT719573.1 Bacillus infantis strain MER_TA_169 16S ribosomal RNA gene, partial sequence
GTCGAGCGGACGGATGGGAGCTTGCTCCCTGAAGTCAGCGGCGGACGGGTGAGTAACACGTGGGCAACCTGCCTGTAAGACTGGGATAACTTCGGGAAACCGGAGCTAATACCGGATAATGCACAGCCTCTCATGAGGCTATGCTGAAAGATGGTTTCGGCTATCACTTACAGATGGGCCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAACTCTGTTGTCAGGGAAGAACAAGTGCCGGAGTAACTGCCGGCACCTTGACGGTACCTGACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTCTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGGACTTGAGTGCAGAAGAGGAAAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTTTCTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGCAGCAAACGCATTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCTCCTGACAACCCTAGAGATAGGGCGTTCCCCTTCGGGGGACAGGATGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTAGGTTGGGCACTCTAAGGTGAC
TGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCC CCTTATGACCTGGGCTACACACGTGCTACAATGGATGGTACAAAGGGCTG CAAGACCGCGAGGTTAAGCGAATCCCATAAAACCATTCTCAGTTCGGATT GCAGGCTGCAACTCGCCTGCATGAAGCTGGAATCGCTAGTAATCGCGGAT CAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCAC ACCACGAGAGTTTGTAACACCCGAAGTCGGTGGGGTAACCTTTGGAGCCA GCCGC
>HQ397586.1 Bacillus firmus strain BSCS3 16S ribosomal RNA gene, partial sequence
ACATGCAGTCGAGCGGACGGATGGGAGCTTGCTCCCTGAAGTCAGCGGCGGACGGGTGAGTAACACGTGGGCAACCTGCCTGTAAGACTGGGATAACTTCGGGAAACCGGAGCTAATACCGGATAATGCACAGCCTCTCATGAGGCTATGCTGAAAGATGGTTTCGGCTATCACTTACAGATGGGCCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAACTCTGTTGTCAGGGAAGAACAAGTGCCGGAGTAACTGCCGGCACCTTGACGGTACCTGACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTCTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGGACTTGAGTGCAGAAGAGGAAAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTTTCTGGTCTGTAAC
TGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGG TAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTT TAGTGCTGCAGCAAACGCATTAAGCACTCCGCCTGGGGAGTACGGCCGCA AGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCAT GTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCTCC TGACAACCCTAGAGATAGGGCGTTCCCCTTCGGGGGACAGGATGACAGGT GGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGC AACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAA GGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCAT CATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGATGGTACAAAG GGCTGCAAGACCGCGAGGTTAAGCGAATCCCATAAAACCATTCTCAGTTC GGATTGCAGGCTGCAACTCGCCTGCATGAAGCTGGAATCGCTAGTAATCG CGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCC GTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGGGGTAACCTTTGG AGCC
>KJ000207.1 Bacillus thuringiensis strain VKK-GA1 16S ribosomal RNA gene, partial sequence
CGGACGGGTGAGTAACACGTGGGCAACCTGCCTGTAAGACTGGGATAACTTCGGGAAACCGGAGCTAATACCGGATAATGCACAGCCTCTCATGAGGCTATGCTGAAAGATGGTTTCGGCTATCACTTACAGATGGGCCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCA
ACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAACTCTGTTGTCAGGG AAGAACAAGTGCCGGAGTAACTGCCGGCACCTTGACGGTACCTGACCAGA AAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAA GCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTCTCTTAAGT CTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGG GACTTGAGTGCAGAAGAGGAAAGTGGAATTCCACGTGTAGCGGTGAAATG CGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTTTCTGGTCTGTA ACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCT GGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCC TTTAGTGCTGCAGCAAACGCATTAAGCACTCCGCCTGGGGAGTACGGCCG CAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAG CATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATC TCCTGACAACCCTAGAGATAGGGCGTTCCCCTTCGGGGGACAGGATGACA GGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCC CGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTAGGTTGGGCACTC TAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAT CATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGATGGTACA AAGGGCTGCAAGACCGCGAGGTTAAGCGAATCCCATAAAACCATTCTCAG TTCGGATTGCAGGCTGCAACTCGCCTGCATGAAGCTGGAATCGCTAGTAAT CGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTA
>KM041133.1 Bacillus drentensis strain G5C_0m_04 16S ribosomal RNA gene,partial sequence
TGCAGTCGAGCGGACGGATGGGAGCTTGCTCCCTGAAGTCAGCGGCGGAC GGGTGAGTAACACGTGGGCAACCTGCCTGTAAGACTGGGATAACTTCGGG AAACCGGAGCTAATACCGGATAATGCATAACCTCTCATGAGGTTATGCTG AAAGATGGTTTCGGCTATCACTTACAGATGGGCCCGCGGCGCATTAGCTAG TTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAG GGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGG CAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCC GCGTGAGTGATGAAGGTTTTCGGATCGTAAAACTCTGTTGTCAGGGAAGA ACAAGTGCCGGAGTAACTGCCGGCACCTTGACGGTACCTGACCAGAAAGC CACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGT TGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTCTCTTAAGTCTGA TGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGGACT TGAGTGCAGAAGAGGAAAGTGGAATTCCACGTGTAGCGGTGAAATGCGTA GAGATGTGGAGGAACACCAGTGGCGAAGGCGACTTTCTGGTCTGTAACTG ACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTA GTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTA GTGCTGCAGCAAACGCATTAAGCACTCCGCCTGGGGAGTACGGCCGCAAG GCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGT GGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCTCCTG ACAACCCTAGAGATAGGGCTTTCCCCTTCGGGGGACAGGATGACAGGTGG TGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAA CGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGG TGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCA TGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGATGGTACAAAGG GCTGCAAGACCGCGAGGTTAAGCGAATCCCATAAAACCATTCTCAGTTCG GATTGCAGGCTGCAACTCGCCTGCATGAAGCTGGAATCGCTAGTAATCGC GGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCG TCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGGGGTAACCTTTGGA GCCA
>KT274776.1 Bacillus licheniformis strain DMB31 16S ribosomal RNA gene,partial sequence
GGATGCTTGATTGAACCGCATGGTTCAATTATAAAAGGTGGCTTTTAGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAACTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGCAGCAAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAACCCTAGAGATAGGGCTTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGGCAGAACAAAGGGCAGCGAAGCCGCGAGGCTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCG
>KP296232.1 Bacillus sonorensis strain Na5RB-2 16S ribosomal RNA gene, partial sequence
ATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGCTTGATTGAACCGCATGGTTCAATTATAAAAGGTGGCTTTTAGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAACTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGCAGCAAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGC
AACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAACCCTAGAGAT AGGGCTTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCA GCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGA TCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAA ACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTG GGCTACACACGTGCTACAATGGGCAGAACAAAGGGCAGCGAAGCCGCGA GGCTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAAC TCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCG GTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTT TGTAACACCCGAAGTCGGTGAGGTAACCTTTG
>EU833948.1 Pseudomonas putida strain FWC30 16S ribosomal RNA gene, partial sequence
GAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGGTTGTTTGAACCGCATGGTTCAAACATAAAAGGTGGCTTYGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCA
ACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGA GTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACC AGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGC GTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGAT GAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCAT
>KR010178.1 Bacillus amyloliquefaciens strain NSB-1 16S ribosomal RNA gene, partial sequence
GTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGGTTGTCTGAACCGCATGGTTCAGACATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTGCCGTTCAAATAGGGCGGCACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAAC
GATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACG CATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGG AATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGC AACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAATCCTAGAGATA GGACGTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAG CTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGAT CTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAA CCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGG GCTACACACGTGCTACAATGGACAGAACAAAGGGCAGCGAAACCGCGAG GTTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACT CGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCACATGCCGGGGG GGAAAAAAACTTTCCCCGGGCCTTTGTACACACCGGCCCGTCACACCACG AAGAGTTTGTAACACCCCGAAGTCCGGTGAGGTAACCTTTTATGGAGCCA GCCGCCCGAAGGTGGGACAGATGATTGGGGTGATAG
>KT216570.1 Bacillus methylotrophicus strain SHP2-20 16S ribosomal RNA gene, partial sequence
TCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGGTTGTCTGAACCGCATGGTTCAGACATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTT
CCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGT TTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTGCCGTTCAAATA GGGCGGCACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGC CAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGG CGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCT CAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGA GAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACA CCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAA GCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACG ATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGC ATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGA ATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCA ACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAATCCTAGAGATAG GACGTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCT CGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCT TAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACC GGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGC TACACACGTGCTACAATGGACAGAACAAAGGGCAGCGAAACCGCGAGGTT AAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGA CTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCCGGGGGA ATACCGTTCCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTG TAACACCCGAAGTCGGTGAGGTAACCTTTTTAGGAGCCAGCCGCCGAAGG TGGGACAGATGATTGGGGTGA
>LN890068.1 Bacillus pseudomycoides partial 16S rRNA gene, strain L72
TCGAGCGAACCGATTAAGAGCTTGCTCTTATGAAGTTAGCGGCGGACGGG TGAGTAACACGTGGGTAACCTGCCCATAAGACTGGGATAACTCCGGGAAA CCGGGGCTAATACCGGATAACATTTTGCACCGCATGGTGCGAAATTGAAA GGCGGCTTCGGCTGTCACTTATGGATGGACCCGCGTCGCATTAGCTAGTTG GTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGT GATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAG CAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCG TGAGTGATGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACA AGTGCTAGTTGAATAAGCTGGCACCTTGACGGTACCTAACCAGAAAGCCA CGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTA TCCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGGTTTCTTAAGTCTGATG TGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGAGACTTG AGTGCAGAAGAGGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGA GATATGGAGGAACACCAGTGGCGAAGGCGACTTTCTGGTCTGTAACTGAC ACTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGT CCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGT GCTGAAGTTAACGCATTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGC TGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGG TTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGAC AACCCTAGAGATAGGGCTTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCA TGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAG CGCAACCCTTGATCTTAGTTGCCATCATTAAGTTGGGCACTCTAAGGTGAC TGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCC CCTTATGACCTGGGCTACACACGTGCTACAATGGACGGTACAAAGAGCTG CAAGACCGCGAGGTGGAGCTAATCTCATAAAACCGTTCTCAGTTCGGATTG TAGGCTGCAACTCGCCTACATGAAGCTGGAATCGCTAGTAATCGCGGATC AGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACA CCACGAGAGTTTGTAACACCCGAAGTCGGTGGGGTAACCTTTT
>KF419123.1 Bacillus megaterium strain QAP17 16S ribosomal RNA gene,partial sequence
CGTGCGCGCTATAATGCAAGTCGAGCGAACTGATTAGAAGCTTGCTTCTATGACGTTAGCGGCGGACGGGTGAGTAACACGTGGGCAACCTGCCTGTAAGACTGGGATAACTTCGGGAAACCGAAGCTAATACCGGATAGGATCTTCTCCTTCATGGGAGATGATTGAAAGATGGTTTCGGCTATCACTTACAGATGGGCCCGCGGTGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTACGAGAGTAACTGCTCGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGAAAAGCGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGGCTTTTTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAACTCTAGAGATAGAGCGTTCCCCTTCGGGGGGACAGAGTTGACAGGTGGGTGCATGGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGGCTTAAGTTCCGCCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTTAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACG
TGCTACAATGGATGGTACAAAGGGCTGCAAGACCGCGAGGTCAAGCCAAT CCCATAAAACCATTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGA AGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTC CCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGA AGTCGGTGGAGTAACCGTAAGGAGCTAGCCGCCTAAGGTGGGACAGATGA TTGGGGGAAGTCGAACAAGTGCG
>KP706808.1 Bacillus aryabhattai strain SO4H51 16S ribosomal RNA gene, partial sequence
CTTCTATGACGTTAGCGGCGGACGGGTGAGTAACACGTGGGCAACCTGCCTGTAAGACTGGGATAACTTCGGGAAACCGAAGCTAATACCGGATAGGATCTTCTCCTTCATGGGAGATGATTGAAAGATGGTTTCGGCTATCACTTACAGATGGGCCCGCGGTGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTACGAGAGTAACTGCTCGTACCTTGACGGTACCTTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGAAAAGCGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGGCTTTTTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTA
AGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAACTCTAGAGATAGAGCGTTCCCCTTCGGGGGGACAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTTAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGATGGTACAAAGGGCTGCAAGACCGCGAGGTCAAGCCAATCCCATAAAACCATTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAAC
Blast và xác định tên loài của mẫu 1
Trình tự copy từ Chromas:
TGCCCACGGTAGAGCTTAGCGTCTATGAAGTTAGCGTGCTGGACGGTGTGA GCTAACACGTGGTGACAACCTGCCTGTAAGACTGGGATAACTTCGGGAAA CCGAAGCTAATACCGGATAGGATCTTCTCCTTCATGGGAGATGATTGAAAG ATGGTTTCGGCTATCACTTACAGATGGGCCCGCGGTGCATTAGCTAGTTGG TGAGGTAACGGCTCACCAAGGCAACGATGCATAGCCGACCTGAGAGGGTG ATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGC AGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGT GAGTGATGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAA GTACAAGAGTAACTGCTTGTACCTTGACGGTACCTAACCAGAAAGCCACG GCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATC CGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTTTCTTAAGTCTGATGTG AAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGAACTTGAG TGCAGAAGAGAAAAGCGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGA TGTGGAGGAACACCAGTGGCGAAGCGGCTTTTTGGTCTGTAACTGACGCTG AGGCGCGAAAGCGTGGGGGAGCA
Kết quả hiện ra ta xác định được tên loài là Priestia megaterium.
Hình 3.1 Kết quả BLAST trình tự mẫu 1.
Xây dựng cây di truyền các accession number
Cây phả hệ mô tả lịch sử tiến hóa của một nhóm các loài với những đặc tính khác nhau nhưng cùng có mối quan hệ họ hàng với nhau và cùng hình thành từ một tổ tiên chung trong quá khứ.
Hình 3.2 Cây di truyền từ trình tự của 18 loài.
Từ Hình 3.2 cho thấy có thể thấy các Accession Number từ đề có cấu trúc DNA thuộc chi Bacillus.
3.3.2 Cây di truyền từ trình tự của mẫu 1
Hình 3.3 Cây di truyền từ trình tự của mẫu 1.
