Research on the ability of propagation of some polyscias fruticosa varieties by stem cutting and in vitro

VIETNAM NATIONAL UNIVERSITY OF AGRICULTURE FACULTY OF AGRONOMY  UNDERGRADUATE THESIS TOPIC: RESEARCH ON THE ABILITY OF PROPAGATION OF SOME Polyscias fruticosa VARIETIES BY STEM CUTTING AND IN VITRO Instructor: Assoc Prof Dr Ninh Thi Phip Department: Industrial and Medicinal Crops Student: Duong Thu Hang Class: K61KHCTT Course: 2016 – 2021 Location: Vietnam National University of Agriculture Hanoi – 2021 COMMITMENT I pledge that the contents of this report are honestly conducted by me under the guidance of Assoc Prof Dr Ninh Thi Phip and MSc Nguyen Thi Son I hereby undertake that all references in this document are quoted which are the name of the authors, name of researches, time, location, and origin Hanoi, January 25, 2021 Student Duong Thu Hang ACKNOWLEDGE During the implement this graduation thesis, I am not the only effort but also received a lot of support in many aspects of leaders, organizations, and individuals First of all, I would like to express my sincere thanks to the dean of the Agronomy Faculty in general, my teachers in the Department of Industrial and Medicinal Crops in particular who taught and created most conditions for students in the process of learning and performing this research The project would not have been possible if it lacks support from my advisors Therefore, I would like to express my deep gratitude and respect to Assoc Prof Dr Ninh Thi Phip, MSc Nguyen Thi Son has dedicatedly instructed, cared for, and helped me during the last time Their ideas, inputs, and guidance were most helpful and have assisted me in making the research Moreover, I also would like to thank the dean and all the teachers and sisters in the Institute of Agricultural Biology for imparting my knowledge, facilitating and supporting me during the implementation of the topic This is a great opportunity I was able to practice my skills in class At the same time, it was good to help to give me more confidence in myself Finally, I would like to send my sincere thanks to my family, friends, and relatives for encouraging, supporting, and helping me during the thesis Thank you again for your support Hanoi, January 25, 2021 Sincerely, Duong Thu Hang LIST OF CONTENTS COMMITMENT ACKNOWLEDGE LIST OF CONTENTS LIST OF TABLES LIST OF PICTURES LIST OF ABBREVIATION ABSTRACT 10 PART I INTRODUCTION 13 1.1 Background and significance 13 1.2 Objective and requirements 14 1.2.1 Objective 14 1.2.2 Requirements 14 PART II LITERATURE REVIEWS 15 2.1 General introduction of Polyscias fruticosa 15 2.1.1 Classification and distribution of Polyscias fruticosa 15 2.1.2 Botanical characteristics 15 2.1.3 Environmental factors influence growth and development 16 2.1.4 Chemical composition 16 2.1.5 Pharmacological benefits and uses 17 2.2 Production and consumption of Polyscias fruticosa in Vietnam and the world 19 2.2.1 Production and consumption of Polyscias fruticosa in Vietnam 19 2.2.2 Production and consumption of Polyscias fruticosa in the world 22 2.3 Some studies about Polyscias fruticosa 23 2.3.1 Scientific studies on chemical composition and pharmacological effects 23 2.3.2 Studies on breeding, propagation, and cultivation 24 2.4 Cultivation techniques 27 2.5 Conclusion 29 PART III MATERIAL AND METHOD 33 3.1 Plant and materials 33 3.2 Time and location 33 3.3 Research contents 33 3.4 Research method 34 3.4.1 Experiment 1: Evaluation on morphological characteristics of some Polyscias fruticosa varieties 34 3.4.2 Experiment 2: Research on the in vitro propagation of some Polyscias fruticosa varieties 34 3.4.3 Experiment 3: Researches on propagation of VT-DL1, and VT-DL2 by cutting 36 3.5 Parameters collecting 37 3.5.1 Parameter of morphological characteristics 37 3.5.2 Parameters of the in vitro propagation of some Polyscias fruticosa varieties 37 3.5.3 Parameters of the propagation of some Polyscias fruticosa by stem cutting 39 3.6 Data analysis method 39 PART IV: RESULTS AND DISCUSSION 40 4.1 Evaluation on morphological characteristics of some Polyscias fruticosa varieties 40 4.1.1 Stem morphological characteristics 40 4.1.2 Leaf morphological characteristics 41 4.1.3 Root morphological characteristics 43 4.2 Research on the in vitro propagation of some Polyscias fruticosa varieties 43 4.2.1 Evaluating the effect of varieties on survival rate and regenerate rate at initiation stage Error! Bookmark not defined 4.2.2 Evaluating the effect of coconut water concentration on multiplication stage of three Polyscias fruticosa varieties 46 4.2.3 Evaluating the effect of αNAA and IBA regulator on the rooting ability of three Polyscias fruticosa varieties 53 4.2.4 Evaluating the effect of varieties on seedling survival rate at acclimatization stage 58 4.3 Researches on the propagation of VT-DL1, and VT-DL2 by stem cutting 59 4.3.1 Evaluating the effect of branch positions on rooting rate, shooting rate and transplanting rate of the propagation of VT-DL1 and VT-DL2 by stem cutting 59 4.3.2 Evaluating the effect of branch positions on some growth and development indicators of the propagation of VT-DL1 and VT-DL2 by stem cutting 63 PART V CONCLUSION AND RECOMMENDATION 66 5.1 Conclusion 66 5.2 Recommendation 67 PART VI REFERENCES 68 DATA ANALYSIS 71 LIST OF TABLES Table 4.1 Stem morphological characteristics of some Polyscias fruticosa varieties Table 4.2 Leaf morphological characteristics of some Polyscias fruticosa varieties Table 4.3 Root morphological characteristics of some Polyscias fruticosa varieties Table 4.4 The effect of varieties on survival rate and regenerate rate at the initiation stage Table 4.5 The effect of coconut water concentration on multiplication stage of three Polyscias fruticosa varieties after weeks Table 4.6 The effect of αNAA and IBA regulator on the rooting ability of three Polyscias fruticosa varieties Table 4.7 The effect of varieties on seedling survival rate at acclimatization stage after weeks Table 4.8 Effect of branch positions on rooting rate, shooting rate and transplanting rate of the propagation of VT-DL1 and VT-DL2 by stem cutting Table 4.9 The effect of branch positions on some growth and development indicators of the propagation of VT-DL1 and VT-DL2 by stem cutting method after 60 days’ transplanting LIST OF PICTURES Picture 4.1 Survival explants of some Polyscias fruticosa varieties Picture 4.2 Infected samples of some Polyscias fruticosa varieties Picture 4.3 The effect of coconut water concentration on multiplication stage of three Polyscias fruticosa varieties Picture 4.4 The effect of αNAA and IBA regulator on the rooting ability of three Polyscias fruticosa varieties Picture 4.5 Effect of branch positions on rooting rate, shooting rate and transplanting rate of the propagation of VT-DL1 and VT-DL2 by cutting Picture 4.6 The seedling in the nursery LIST OF ABBREVIATION MS Murashige & Shoog, 1962 BAP or BA 6- Benzylaminopurine IBA Indolylbutyrique acid Ki Kinetin αNAA or NAA 1-Naphthylacetic Acid LSD Least Significant Difference CV Correlation of Variants ∑ Sum ABSTRACT We conduct the topic: “Research on the ability of propagation of some Polyscias fruticosa by stem cutting and in vitro" to compare two different propagation methods Research methods: conduct experiments, with factors are varieties and treatments Experiment 1: Evaluation of morphological characteristics of some Polyscias fruticosa varieties Experiment 2: Research on the in vitro propagation of some Polyscias fruticosa varieties Experiment 3: Researches on the propagation of VT-DL1, and VT-DL2 by cutting The experiment is conducted following the Randomized Complete Block Design of replications for each treatment Conclusions: VT-DL1 is the tri-pinnately compound leaf, has a small and lanceolate form, and the leaf margin is lobed, irregularly serrate VT-DL2 is a pinnately compound leaf, has a big and ovate form; the leaf margin is irregularly serrate VT-DL3 is a very thick leaf, pinnately compound leaf, and has a slightly lobed, regularly serrate, and reddish color The in vitro propagation of Polyscias fruticosa varieties at stages (initiation stage, multiplication, rooting stage, and acclimatization stage), each variety illustrated the different results + In the initiation stage, VT-DL2 is the best both survived and regenerated, with 83.33% and 82.22%, respectively + In the multiplication stage, treatment MS+ 1.5mg/l BA + 0.5 mg/l Ki + 0.5 mg/l IBA + 15% coconut water (M4) is the best choice for multiplication stage of some Polyscias fruticosa by in vitro propagation + In the rooting stage, treatment R2 (½ MS + 0.5 mg/l IBA + 0.1g/l activated carbon + 10% coconut water) was the best choice for VT-DL1 at the rooting stage, and treatment R1 (½ MS + mg/l NAA + 0.1 g/l activated carbon + 10% coconut water) was the best choice for rooting stage of VT-DL2 and VT-DL3 + In the acclimatization stage, VT-DL2 is still the strongest variety, giving the largest survival rate of 86.67% Cuttings propagation by using a middle branch gave the best choice for VT-DL1 and VT-DL2 10 SQUARES SQUARES LN ============================================================================= NL$ 33.3333 16.6667 0.56 0.595 G$ 555.555 555.555 18.52 0.002 BP$ 233.333 116.667 3.89 0.056 G$*BP$ 77.7778 38.8889 1.30 0.316 * RESIDUAL 10 300.000 30.0000 * TOTAL (CORRECTED) 17 1200.00 70.5882 BALANCED ANOVA FOR VARIATE STEM FILE NR 28/ 2/21 7:32 :PAGE Dinh lang giam canh VARIATE V007 STEM LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= NL$ 723334E-02 361667E-02 0.87 0.452 G$ 312500E-01 312500E-01 7.51 0.020 BP$ 816133 408067 98.01 0.000 G$*BP$ 640000E-02 320000E-02 0.77 0.492 * RESIDUAL 10 416332E-01 416332E-02 * TOTAL (CORRECTED) 17 902650 530971E-01 BALANCED ANOVA FOR VARIATE HEIGHT FILE NR 28/ 2/21 7:32 :PAGE Dinh lang giam canh VARIATE V008 HEIGHT LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= NL$ 2.37880 1.18940 3.43 0.072 G$ 53.3889 53.3889 154.08 0.000 BP$ 165.495 82.7475 238.82 0.000 G$*BP$ 521244 260622 0.75 0.500 * RESIDUAL 10 3.46492 346492 * TOTAL (CORRECTED) 17 225.249 13.2499 BALANCED ANOVA FOR VARIATE SHOOT FILE NR 28/ 2/21 7:32 :PAGE Dinh lang giam canh VARIATE V009 SHOOT LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= NL$ 217778 108889 0.51 0.619 G$ 59.7689 59.7689 280.75 0.000 BP$ 8.03111 4.01556 18.86 0.000 G$*BP$ 8.88444 4.44222 20.87 0.000 * RESIDUAL 10 2.12888 212888 * TOTAL (CORRECTED) 17 79.0311 4.64889 BALANCED ANOVA FOR VARIATE LEAVES FILE NR 28/ 2/21 7:32 :PAGE Dinh lang giam canh VARIATE V010 LEAVES LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= NL$ 1.28444 642222 2.49 0.132 G$ 98.0000 98.0000 379.52 0.000 BP$ 30.4844 15.2422 59.03 0.000 G$*BP$ 68.8134 34.4067 133.24 0.000 72 * RESIDUAL 10 2.58222 258222 * TOTAL (CORRECTED) 17 201.164 11.8332 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE NR 28/ 2/21 7:32 :PAGE Dinh lang giam canh MEANS FOR EFFECT NL$ NL$ SE(N= 5%LSD NOS 6 6) 10DF NL$ NOS 6 ROOTR 90.0000 88.3333 85.0000 SPROUTR 83.3333 86.6667 81.6667 TRANSR 86.6667 88.3333 85.0000 2.06380 6.50310 2.06380 6.50310 2.23607 7.04593 HEIGHT 26.9467 26.3167 27.1767 SHOOT 5.23333 5.50000 5.40000 LEAVES 12.7667 12.7667 13.3333 STEM 1.25833 1.25667 1.30000 0.263417E-01 0.830037E-01 SE(N= 6) 0.240309 0.188365 0.207454 5%LSD 10DF 0.757224 0.593544 0.653694 MEANS FOR EFFECT G$ G$ DL2 DL3 SE(N= 5%LSD NOS 9 9) 10DF G$ DL2 DL3 NOS 9 ROOTR 82.2222 93.3333 SPROUTR 78.8889 88.8889 TRANSR 81.1111 92.2222 1.68508 5.30976 1.68508 5.30976 1.82574 5.75298 HEIGHT 25.0911 28.5356 SHOOT 3.55556 7.20000 LEAVES 10.6222 15.2889 STEM 1.23000 1.31333 0.215079E-01 0.677723E-01 SE(N= 9) 0.196212 0.153799 0.169385 5%LSD 10DF 0.618270 0.484627 0.533739 MEANS FOR EFFECT BP$ BP$ Y M O SE(N= 5%LSD NOS 6 6) 10DF ROOTR 90.0000 91.6667 81.6667 SPROUTR 83.3333 90.0000 78.3333 TRANSR 88.3333 90.0000 81.6667 2.06380 6.50310 2.06380 6.50310 2.23607 7.04593 STEM 0.988333 1.32500 1.50167 0.263417E-01 0.830037E-01 Initiation stage BALANCED ANOVA FOR VARIATE SURR FILE SURV 2/ 3/21 22:56 :PAGE Survival rate and regenerate rate VARIATE V003 SURR LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= NL$ 32.0864 16.0432 3.25 0.145 G$ 195.146 97.5728 19.78 0.010 * RESIDUAL 19.7284 4.93211 * TOTAL (CORRECTED) 246.961 30.8701 - 73 BALANCED ANOVA FOR VARIATE REGR FILE SURV 2/ 3/21 22:56 :PAGE Survival rate and regenerate rate VARIATE V004 REGR LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= NL$ 7.39261 3.69630 2.00 0.250 G$ 229.570 114.785 62.11 0.002 * RESIDUAL 7.39258 1.84815 * TOTAL (CORRECTED) 244.356 30.5445 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE SURV 2/ 3/21 22:56 :PAGE Survival rate and regenerate rate MEANS FOR EFFECT NL$ NL$ NOS 3 SURR 76.6667 81.1100 77.7767 REGR 76.6667 77.7767 75.5567 SE(N= 3) 1.28220 0.784888 5%LSD 4DF 5.02595 3.07659 MEANS FOR EFFECT G$ G$ DL1 DL2 DL3 NOS 3 SURR 80.0000 83.3333 72.2200 REGR 77.7800 82.2200 70.0000 SE(N= 3) 1.28220 0.784888 5%LSD 4DF 5.02595 3.07659 ANALYSIS OF VARIANCE SUMMARY TABLE FILE SURV 2/ 3/21 22:56 :PAGE Survival rate and regenerate rate F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE SURR REGR GRAND MEAN (N= 9) NO OBS 78.518 76.667 STANDARD DEVIATION C OF V |NL$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 5.5561 2.2208 2.8 0.1452 5.5267 1.3595 1.8 0.2501 |G$ | | | 0.0103 0.0020 | | | | Multiplication stage BALANCED ANOVA FOR VARIATE RESRATE FILE MULTI 2/ 3/21 23: :PAGE MULTIPLICATION STAGE VARIATE V004 RESRATE LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= NL$ 16.6667 8.33333 0.44 0.657 VAR$ 50.0000 25.0000 1.31 0.290 T$ 570.304 190.101 9.95 0.000 VAR$*T$ 164.733 27.4556 1.44 0.245 * RESIDUAL 22 420.408 19.1094 74 * TOTAL (CORRECTED) 35 1222.11 34.9175 BALANCED ANOVA FOR VARIATE SHOOT FILE MULTI 2/ 3/21 23: :PAGE MULTIPLICATION STAGE VARIATE V005 SHOOT LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= NL$ 1.11642 558211 0.56 0.586 VAR$ 44.7749 22.3875 22.31 0.000 T$ 206.565 68.8550 68.62 0.000 VAR$*T$ 22.0199 3.66998 3.66 0.011 * RESIDUAL 22 22.0763 1.00347 * TOTAL (CORRECTED) 35 296.552 8.47293 BALANCED ANOVA FOR VARIATE MULTI FILE MULTI 2/ 3/21 23: :PAGE MULTIPLICATION STAGE VARIATE V006 MULTI LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= NL$ 110889E-01 554444E-02 0.55 0.589 VAR$ 448606 224303 22.27 0.000 T$ 2.06428 688093 68.32 0.000 VAR$*T$ 217572 362620E-01 3.60 0.012 * RESIDUAL 22 221578 100717E-01 * TOTAL (CORRECTED) 35 2.96312 846606E-01 BALANCED ANOVA FOR VARIATE HEIGHT FILE MULTI 2/ 3/21 23: :PAGE MULTIPLICATION STAGE VARIATE V007 HEIGHT LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= NL$ 561668E-02 280834E-02 1.00 0.384 VAR$ 5.73315 2.86657 ****** 0.000 T$ 4.78172 1.59391 570.01 0.000 VAR$*T$ 284872 474787E-01 16.98 0.000 * RESIDUAL 22 615177E-01 279626E-02 * TOTAL (CORRECTED) 35 10.8669 310482 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE MULTI 2/ 3/21 23: :PAGE MULTIPLICATION STAGE MEANS FOR EFFECT NL$ NL$ NOS 12 12 12 RESRATE 83.3333 82.5000 84.1667 SHOOT 24.3617 24.2500 24.6667 MULTI 2.43667 2.42500 2.46667 HEIGHT 2.97750 3.00167 2.97333 SE(N= 12) 1.26192 0.289175 0.289708E-01 0.152650E-01 5%LSD 22DF 3.70103 0.848106 0.849670E-01 0.447700E-01 MEANS FOR EFFECT VAR$ - 75 VAR$ NOS 12 12 12 DL1 DL2 DL3 RESRATE 82.5000 85.0000 82.5000 SHOOT 23.0000 24.5558 25.7225 MULTI 2.30000 2.45583 2.57250 HEIGHT 3.42167 2.45667 3.07417 SE(N= 12) 1.26192 0.289175 0.289708E-01 0.152650E-01 5%LSD 22DF 3.70103 0.848106 0.849670E-01 0.447700E-01 MEANS FOR EFFECT T$ T$ NOS 9 9 CT1 CT2 CT3 CT4 RESRATE 78.1489 81.4811 84.8144 88.8889 SHOOT 20.8889 23.5933 26.2967 26.9256 MULTI 2.08889 2.36000 2.63000 2.69222 HEIGHT 2.54556 2.75444 3.14111 3.49556 SE(N= 9) 1.45714 0.333911 0.334526E-01 0.176266E-01 5%LSD 22DF 4.27358 0.979308 0.981115E-01 0.516960E-01 MEANS FOR EFFECT VAR$*T$ VAR$ DL1 DL1 DL1 DL1 DL2 DL2 DL2 DL2 DL3 DL3 DL3 DL3 T$ CT1 CT2 CT3 CT4 CT1 CT2 CT3 CT4 CT1 CT2 CT3 CT4 SE(N= 5%LSD NOS 3 3 3 3 3 3 3) 22DF VAR$ DL1 DL1 DL1 DL1 DL2 DL2 DL2 DL2 DL3 DL3 DL3 DL3 T$ CT1 CT2 CT3 CT4 CT1 CT2 CT3 CT4 CT1 CT2 CT3 CT4 NOS 3 3 3 3 3 3 RESRATE 78.8900 80.0000 84.4433 86.6667 75.5567 84.4433 85.5567 94.4433 80.0000 80.0000 84.4433 85.5567 SHOOT 20.4433 22.8900 23.8900 24.7767 21.0000 23.5567 25.8900 27.7767 21.2233 24.3333 29.1100 28.2233 2.52385 7.40206 0.578350 1.69621 MULTI 2.04333 2.29000 2.39000 2.47667 2.10000 2.35667 2.59000 2.77667 2.12333 2.43333 2.91000 2.82333 0.579417E-01 0.169934 HEIGHT 2.91000 3.19000 3.62333 3.96333 2.11000 2.32333 2.61000 2.78333 2.61667 2.75000 3.19000 3.74000 SE(N= 3) 0.305301E-01 5%LSD 22DF 0.895401E-01 ANALYSIS OF VARIANCE SUMMARY TABLE FILE MULTI 2/ 3/21 23: :PAGE MULTIPLICATION STAGE F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE RESRATE SHOOT MULTI GRAND MEAN (N= 36) NO OBS 36 83.333 36 24.426 36 2.4428 STANDARD DEVIATION C OF V |NL$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 5.9091 4.3714 5.2 0.6570 2.9108 1.0017 4.1 0.5862 0.29096 0.10036 4.1 0.5894 |VAR$ | | | 0.2905 0.0000 0.0000 |T$ | | | 0.0003 0.0000 0.0000 |VAR$*T$ | | | 0.2452 0.0114 0.0123 | | | | 76 HEIGHT 36 2.9842 0.55721 0.52880E-01 1.8 0.3843 0.0000 0.0000 0.0000 Rooting stage BALANCED ANOVA FOR VARIATE %ROOTING FILE RE 21/ 2/21 21:15 :PAGE Rooting stage of some Polycias spp VARIATE V004 %ROOTING LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= NL 140.741 70.3704 3.45 0.056 DL$ 385.185 192.593 9.45 0.002 TR$ 185.185 92.5926 4.55 0.027 DL$*TR$ 1525.93 381.481 18.73 0.000 * RESIDUAL 16 325.926 20.3704 * TOTAL (CORRECTED) 26 2562.96 98.5755 BALANCED ANOVA FOR VARIATE N-ROOT FILE RE 21/ 2/21 21:15 :PAGE Rooting stage of some Polycias spp VARIATE V005 N-ROOT LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= NL 331852 165926 1.32 0.295 DL$ 526.190 263.095 ****** 0.000 TR$ 12.1452 6.07259 48.22 0.000 DL$*TR$ 34.7259 8.68148 68.94 0.000 * RESIDUAL 16 2.01484 125928 * TOTAL (CORRECTED) 26 575.407 22.1311 BALANCED ANOVA FOR VARIATE LENGHT FILE RE 21/ 2/21 21:15 :PAGE Rooting stage of some Polycias spp VARIATE V006 LENGHT LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= NL 330667E-01 165334E-01 1.15 0.343 DL$ 30.9948 15.4974 ****** 0.000 TR$ 264089 132044 9.17 0.002 DL$*TR$ 1.88756 471889 32.77 0.000 * RESIDUAL 16 230399 143999E-01 * TOTAL (CORRECTED) 26 33.4099 1.28499 BALANCED ANOVA FOR VARIATE WIDTH FILE RE 21/ 2/21 21:15 :PAGE Rooting stage of some Polycias spp VARIATE V007 WIDTH LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= NL 100119 500593E-01 1.37 0.283 DL$ 72.9438 36.4719 995.33 0.000 TR$ 6.58812 3.29406 89.90 0.000 DL$*TR$ 4.16379 1.04095 28.41 0.000 * RESIDUAL 16 586287 366429E-01 * TOTAL (CORRECTED) 26 84.3821 3.24546 - 77 BALANCED ANOVA FOR VARIATE HEIGHT FILE RE 21/ 2/21 21:15 :PAGE Rooting stage of some Polycias spp VARIATE V008 HEIGHT LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= NL 462519E-01 231259E-01 1.62 0.227 DL$ 1.60501 802504 56.28 0.000 TR$ 442874 221437 15.53 0.000 DL$*TR$ 1.52361 380904 26.71 0.000 * RESIDUAL 16 228149 142593E-01 * TOTAL (CORRECTED) 26 3.84590 147919 BALANCED ANOVA FOR VARIATE N-LEAVES FILE RE 21/ 2/21 21:15 :PAGE Rooting stage of some Polycias spp VARIATE V009 N-LEAVES LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= NL 506667 253333 1.90 0.180 DL$ 22.1867 11.0933 83.20 0.000 TR$ 3.94667 1.97333 14.80 0.000 DL$*TR$ 9.97334 2.49333 18.70 0.000 * RESIDUAL 16 2.13334 133334 * TOTAL (CORRECTED) 26 38.7467 1.49026 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE RE 21/ 2/21 21:15 :PAGE Rooting stage of some Polycias spp MEANS FOR EFFECT NL NL SE(N= 5%LSD NL NOS %ROOTING N-ROOT LENGHT WIDTH 70.0000 9.60000 2.46444 3.68667 73.3333 9.77778 2.53111 3.64667 75.5556 9.86667 2.45111 3.54222 9) 16DF 1.50445 4.51037 NOS HEIGHT 9 0.118288 0.354628 0.399999E-01 0.638078E-01 0.119920 0.191297 N-LEAVES 4.52444 3.71111 4.56000 4.04445 4.62444 3.91111 SE(N= 9) 0.398041E-01 0.121716 5%LSD 16DF 0.119333 0.364907 MEANS FOR EFFECT DL$ DL$ DL1 DL2 DL3 SE(N= 5%LSD DL$ DL1 DL2 DL3 NOS %ROOTING N-ROOT LENGHT WIDTH 67.7778 7.22222 2.77333 3.44000 76.6667 15.9556 3.62444 5.72444 74.4444 6.06667 1.04889 1.71111 9) 16DF 1.50445 4.51037 NOS HEIGHT 9 0.118288 0.354628 0.399999E-01 0.638078E-01 0.119920 0.191297 N-LEAVES 4.84667 4.77778 4.25333 4.24444 4.60889 2.64444 78 SE(N= 9) 0.398041E-01 0.121716 5%LSD 16DF 0.119333 0.364907 MEANS FOR EFFECT TR$ TR$ CT1 CT2 CT3 SE(N= 5%LSD NOS %ROOTING N-ROOT LENGHT WIDTH 76.6667 10.2000 2.48000 3.98222 71.1111 10.2444 2.60444 3.96667 71.1111 8.80000 2.36222 2.92667 9) 16DF TR$ CT1 CT2 CT3 1.50445 4.51037 NOS HEIGHT 9 0.118288 0.354628 0.399999E-01 0.638078E-01 0.119920 0.191297 N-LEAVES 4.61333 3.84444 4.70000 4.37778 4.39556 3.44444 SE(N= 9) 0.398041E-01 0.121716 5%LSD 16DF 0.119333 0.364907 MEANS FOR EFFECT DL$*TR$ DL$ DL1 DL1 DL1 DL2 DL2 DL2 DL3 DL3 DL3 SE(N= 5%LSD TR$ CT1 CT2 CT3 CT1 CT2 CT3 CT1 CT2 CT3 NOS %ROOTING 3 3 3 3 3) 16DF DL$ DL1 DL1 DL1 DL2 DL2 DL2 DL3 DL3 DL3 N-ROOT 60.0000 76.6667 66.6667 86.6667 63.3333 80.0000 83.3333 73.3333 66.6667 2.60579 7.81219 TR$ CT1 CT2 CT3 CT1 CT2 CT3 CT1 CT2 CT3 NOS WIDTH 3 3 3 3 LENGHT 5.46667 2.29333 8.86667 3.32000 7.33333 2.70667 17.9333 3.92000 15.8000 3.52667 14.1333 3.42667 7.20000 1.22667 6.06667 0.966667 4.93333 0.953333 0.204880 0.614234 HEIGHT 3.56000 4.39333 2.36667 6.51333 5.84667 4.81333 1.87333 1.66000 1.60000 0.692818E-01 0.207708 N-LEAVES 4.44667 5.33333 4.76000 4.52000 4.15333 4.08667 4.87333 4.61333 4.34000 3.66667 6.20000 4.46667 5.00000 4.26667 3.46667 2.86667 2.66667 2.40000 SE(N= 3) 0.110518 0.689427E-01 0.210819 5%LSD 16DF 0.331336 0.206691 0.632038 ANALYSIS OF VARIANCE SUMMARY TABLE FILE RE 21/ 2/21 21:15 :PAGE Rooting stage of some Polycias spp F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE (N= 27) NO OBS %ROOTING N-ROOT LENGHT WIDTH HEIGHT N-LEAVES GRAND MEAN STANDARD DEVIATION SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 27 72.963 9.9285 4.5134 27 9.7481 4.7044 0.35486 27 2.4822 1.1336 0.12000 27 3.6252 1.8015 0.19142 27 4.5696 0.38460 0.11941 27 3.8889 1.2208 0.36515 C OF V |NL | | | | | | 6.2 0.0557 3.6 0.2954 4.8 0.3430 5.3 0.2832 2.6 0.2275 6.3 0.1804 |DL$ | |TR$ | | | 0.0020 0.0000 0.0000 0.0000 0.0000 0.0000 | | 0.0270 0.0000 0.0023 0.0000 0.0002 0.0003 |DL$*TR$ | 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 79 Acclimatization stage BALANCED ANOVA FOR VARIATE SURRATE FILE VUON 2/ 3/21 2:24 :PAGE Acclimatization stage VARIATE V003 SURRATE LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= NL$ 266.667 133.333 8.00 0.042 G$ 66.6667 33.3333 2.00 0.250 * RESIDUAL 66.6667 16.6667 * TOTAL (CORRECTED) 400.000 50.0000 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE VUON 2/ 3/21 2:24 :PAGE Acclimatization stage MEANS FOR EFFECT NL$ NL$ NOS 3 SURRATE 83.3333 76.6667 90.0000 SE(N= 3) 2.35702 5%LSD 4DF 9.23902 MEANS FOR EFFECT G$ G$ DL1 DL2 DL3 NOS 3 SURRATE 83.3333 86.6667 80.0000 SE(N= 3) 2.35702 5%LSD 4DF 9.23902 ANALYSIS OF VARIANCE SUMMARY TABLE FILE VUON 2/ 3/21 2:24 :PAGE Acclimatization stage F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE SURRATE GRAND MEAN (N= 9) NO OBS 83.333 STANDARD DEVIATION C OF V |NL$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 7.0711 4.0825 4.9 0.0416 |G$ | | | 0.2501 | | | | 80 81 82 83 84 85 86