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ONLINE Ph.D DISSERTATION INFORMATION The Ph.D Dissertation Title: “The determination of phenolic compounds from the resin of nut oil and the fruit peel of Calophyllum inophyllum useful for quality control with herbal medicines” Specialty: Drug Quality Control Code: 62.73.15.01 Ph.D candidate: HA MINH HIEN Supervisor 1: Assoc Prof Dr Nguyen Khac Quynh Cu Supervisor 2: Dr Nguyen Van Thi Academic institute: University of Medicine and Pharmacy-Ho Chi Minh City SUMMARY OF NEW FINDINGS Phenolic compounds from the resin of nut oil and the fruit peel of Calophyllum inophyllum grown in Vietnam The author describes for the first time the identification of phenolic compounds obtained from the resin of nut oil and the fruit peel of Calophyllum inophyllum grown in Vietnam Three compounds were isolated from the resin of nut oil including 1,5-dihydroxyxanthone, C13H8O4 (8.1 mg per 3.0 kg resin), 4-hydroxyxanthone C13H8O3 (105.0 mg per 3.0 kg resin) and inocalophyllin C (proposed trivial name), C37H48O6 (135.0 mg per 4.0 kg resin) Inocalophyllin C is a novel compound whose structure has been elucidated on the basis of extensive spectroscopic method including UV-Vis, IR, HRMS, EIMS, 1D, 2D NMR together with single crystal X-ray crystallography In the fruit peel of Calophyllum inophyllum, calophyllolide, C26H24O5 (26.0 mg per 4.0 kg fruit peel) was identified Establishment of Chemical Reference Substances For the first time, two chemical reference substances have been established in accordance with the WHO guidelines on establishment, distribution and storage of chemical reference substances They are 4-hydroxyxanthone (9 vials of mg/content: 93.91%) for use in identification tests and calophyllolide (100 vials of 10 mg/content: 95.27%) for use in assays Analytical methods for drug quality control The capillary gas chromatography/mass spectrometric methods for determination of calophyllolide from fruit peel and nut oil resin of Calophyllum inophyllum grown in Vietnam have been developed and validated for use in quality control tests of herbal medicines Likewise, the RP-HPLC-DAD method for determination of calophyllolide from Inopilo oil containing Calophyllum inophyllum oil has been developed and validated for estimating the percentage of calophyllolide This is the first time these techniques for estimating calophyllolide from the resin of nut oil, the oil and the fruit peel of Calophyllum inophyllum have been reported Ho Chi Minh City, March 27, 2014 Ph.D CANDIDATE SUPERVISOR Assoc Prof Dr Nguyen Khac Quynh Cu Dr Nguyen Van Thi RECTOR Ha Minh Hien