© ISO 2017 Traditional Chinese medicine — Panax notoginseng seeds and seedlings Médecine traditionnelle chinoise — Graines et plants de Panax notoginseng INTERNATIONAL STANDARD ISO 20408 First edition[.]
INTERNATIONAL STANDARD ISO 20408 First edition 2017-01 Traditional Chinese medicine — Panax notoginseng seeds and seedlings Médecine traditionnelle chinoise — Graines et plants de Panax notoginseng Reference number ISO 20408:2017(E) © ISO 2017 ISO 20408:2017(E) COPYRIGHT PROTECTED DOCUMENT © ISO 2017, Published in Switzerland All rights reserved Unless otherwise specified, no part o f this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission Permission can be requested from either ISO at the address below or ISO’s member body in the country o f the requester ISO copyright o ffice Ch de Blandonnet • CP 401 CH-1214 Vernier, Geneva, Switzerland Tel +41 22 749 01 11 Fax +41 22 749 09 47 copyright@iso.org www.iso.org ii © ISO 2017 – All rights reserved ISO 20408:2017(E) Contents Page Foreword iv Scope Normative references Terms and definitions Descriptions 4.1 Panax notoginseng seed 4.2 Panax notoginseng seedling Requirements 5.1 5.2 5.3 General characteristics Panax notoginseng seed 5.2.1 Morphological features of seed 5.2.2 Moisture 5.2.6 Fungi 5.2.7 000-seed weight, seed width, seed thickness and seed length Panax notoginseng seedling 5.3.1 Morphological features of seedling 5.3.2 Fungi 5.3.3 Nematode 5.3.4 Seedling weight Purity Viab ility 5 M aturity Sampling 7 Test methods 6.1 6.2 Seed sampling Seedling sampling 7.1 7.2 7.3 7.4 Moisture content Seed width Seed thickness Seed length 000-seed weight Fungi testing 7.9.1 Preparation of seeds 7.9.2 Preparation of seedlings 7.9.3 Alternaria spp testing 7.9.4 Fusarium spp testing Nematode testing Seedling weight Seedling diameter Hibernaculum diameter 7.5 7.6 7.7 7.8 7.9 7.10 7.11 7.12 7.13 M aturity Purity S eed viab ility Test report 9 Packaging, storage and transportation 10 10 Marking 10 Bibliography 11 © ISO 2017 – All rights reserved iii ISO 20408:2017(E) Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies) The work o f preparing International Standards is normally carried out through ISO technical committees Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters o f electrotechnical standardization The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part In particular the different approval criteria needed for the di fferent types o f ISO documents should be noted This document was dra fted in accordance with the editorial rules of the ISO/IEC Directives, Part (see www.iso org/directives) Attention is drawn to the possibility that some o f the elements o f this document may be the subject o f patent rights ISO shall not be held responsible for identi fying any or all such patent rights Details o f any patent rights identified during the development o f the document will be in the Introduction and/or on the ISO list of patent declarations received (see www.iso org/patents) Any trade name used in this document is in formation given for the convenience o f users and does not constitute an endorsement For an explanation on the meaning o f ISO specific terms and expressions related to formity assessment, as well as information about ISO’s adherence to the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following URL: www.iso.org/iso/foreword.html The committee responsible for this document is ISO/TC 249, Traditional Chinese medicine iv © ISO 2017 – All rights reserved INTERNATIONAL STANDARD ISO 20408:2017(E) Traditional Chinese medicine — Panax notoginseng seeds and seedlings Scope T h i s c u ment s p e ci fie s m i n i mu m re qu i rements and te s t me tho d s notoginseng for s e e d s and s e e d l i ngs o f Panax (Burk.) F H Chen It is suitable for marketing of cultivated Panax notoginseng seeds and f f Panax notoginseng s e e d l i ngs I t i s a l s o s u itable to b e u s e d or qua l ity a s s u rance by c u lti vators o Normative references T he fol lowi ng c u ments are re ferre d to i n the tex t i n s uch a way th at s ome or a l l o f thei r content s titute s re qu i rements o f th i s c u ment For date d re ference s , on ly the e d ition cite d appl ie s For u ndate d re ference s , the late s t e d ition o f the re ference d c ument (i nclud i ng a ny amend ments) appl ie s International Seed Testing Association (ISTA): 2016, International Rules for Seed Testing International Seed Testing Association (ISTA): 2003, Working Sheets on Tetrazolium testing Terms and definitions For the pu r p o s e s o f th i s c u ment, the fol lowi ng term s and defi n ition s apply ISO and IEC maintain terminological databases for use in standardization at the following addresses: — IEC Electropedia: available at http://www.electropedia org/ — ISO Online browsing platform: available at http://www.iso org/obp 3.1 000-seed weight average weight o f ever y 0 pu re s e e d s o f a test sample (3.19) 3.2 composite sample p or tion forme d b y combi n i ng a nd m i xi ng a l l the primary samples (3.8) taken from the lot (3.6) 3.3 foreign matter parts of Panax notoginseng seeds (3.11) or seedlings (3.15) other than those named with the limits s p e ci fie d for the herb a l materi a l s concerne d N o te to entr y: Foreign m atter i s a ny orga n i s m , p a r t o r p ro duc t o f a n o rga n i s m , o ther th a n th at n a me d i n the s p e c i fic ation a nd de s c r ip tion o f Panax notoginseng seeds or seedlings N o te to entr y: Fo reign m atter i s m i nera l ad m i x tu re s no t ad her i ng to as soil, stones, sand and dust Panax notoginseng seeds or seedlings, such 3.4 hibernaculum hibernated bud with bud scales © ISO 2017 – All rights reserved ISO 20408:2017(E) 3.5 hibernaculum diameter largest distance at the site of the hibernaculum (3.4) N o te to entr y: S e e Figure N o te to entr y: I t i s e x pre s s e d i n centi me tre s 3.6 lot s p e c i fie d quantity o f seeds (3.11) or seedlings (3.15 ) th at i s phys ic a l ly and u n iquely identi fi able 3.7 maturity p ercentage o f mature s e e d s , de term i ne d b y nu mb er, i n the test sample (3.19) 3.8 primary sample portion taken from the lot (3.6) during one single sampling action 3.9 purity weight of pure seed fraction over the total weight of the test sample (3.19) N o te to entr y: T he pu re s e e d re fers to the s p e c ie s s tate d b y the ap p l ic a nt o r and includes all botanical varieties and cultivars of that species fo u nd to pre dom i n ate i n the te s t N o te to entr y: I t i s e x pre s s e d i n p er cent 3.10 sealed < contai ner for s e e d s > clo s e d i n s uch a way that the contai ner c a n no t b e op ene d to ge t acce s s to the s e e d and b e clo s e d agai n without either de s troyi ng the s e a l or le avi ng evidence o f tamp eri ng N o te to entr y: T h i s de fi n ition re fers to the s e a l i n g o f s e e d 3.11 seed matu re ovu le s pro duce d by Panax notoginseng lots (3.6), as well as of seed samples (Burk.) F H Chen, consisting of three basic parts: embr yo, endo s p erm a nd s e e d co at 3.12 seed length largest distance from the lower part to the top N o te to entr y: S e e Figure N o te to entr y: I t i s e x pre s s e d i n m i l l i me tre s 3.13 seed thickness largest distance from the side perpendicular to the ridge to the opposite side N o te to entr y: S e e Figure N o te to entr y: I t i s e x pre s s e d i n m i l l i me tre s 3.14 seed width largest distance from the side of the raphe to the opposite N o te to entr y: S e e Figure © ISO 2017 – All rights reserved ISO 20408:2017(E) N o te to entr y: I t i s e xpre s s e d i n m i l l i me tre s 3.15 seedling you ng plant o f Panax notoginseng (B u rk.) F H C hen, wh ich, a fter hibernaculum (3.4), tap root (3.18 ) , latera l ro o t a nd fibrou s ro o t c u ltivation for one ye a r, s i s ts o f 3.16 seedling diameter largest distance at the site of the taproot N o te to entr y: S e e Figure N o te to entr y: I t i s e xpre s s e d i n centi me tre s 3.17 seedling weight average weight of test samples (3.19) of seedlings (3.15) 3.18 tap root ro o t generate d from the one ye a r development o f the rad icle a fter s e e d germ i nation 3.19 test sample portion of the composite sample (3.2) to which one of the test required in this standard is applied N o te to entr y: Te s t s a mp le s m ay b e p acke d i n d i fferent m ater i a l s me e ti ng cond ition s moisture or purity (3.9)] fo r s p e c i fic te s ts [e g 3.20 viability p o tenti a l abi l ity o f a s e e d to germ i nate, or c ap abi l ity o f a n embr yo to l ive, a s a p ercentage o f s tai ne d seeds in the test sample (3.19) N o te to entr y: T he p ercentage Topographical Tetrazolium Test 4.1 o f s ta i ne d seeds in the te s t s a mp le shall be e s ti m ate d b y the me tho d of Descriptions Panax notoginseng seed In this document, notoginseng shown in Figure Panax notoginseng seed is the dehyd rate d seed of pl ant Panax (B u rk.) F H C hen s i s ti ng o f th re e b a s ic p ar ts: embr yo, endo s p erm and the s e e d co at, a s © ISO 2017 – All rights reserved ISO 20408:2017(E) a) Faỗade of the seed b) Back of the seed Key seed width seed length seed thickness circular water hole raphe seed coat endosperm emb ryo cavity o f s eed Figure — Structure of Panax notoginseng seed 4.2 Panax notoginseng seedling Panax notoginseng s e e d l i ng i s one -ye ar- old s e e d l i ng , s i s ti ng o f latera l ro o t a nd fibrou s ro o t For prac tic a l appl ic ation, fou r p a r ts: h ib ernac u lu m, tapro o t, Panax notoginseng s e e d l i ngs are cla s s i fie d i nto two group s on the b a s i s o f thei r mor pholo g y: long s e e d l i ngs and s hor t s e e d l i ngs , a s s hown i n Figure © ISO 2017 – All rights reserved ISO 20408:2017(E) Key hibernaculum lateral root taproot fib ro us ro o t seedling diameter hibernaculum diameter Figure — Panax notoginseng seedling Requirements 5.1 General characteristics The following requirements shall be met before separating the composite sample into test samples a) Panax notoginseng seeds shall be clean and free from foreign matter b) Panax notoginseng s e e d l i ngs sha l l b e he a lthy and i ntac t c) T he pre s ence o f mou ldy s e e d s and e xterna l contam i nants wh ich are vi s ible to the na ke d eye s l l not be permitted 5.2 5.2.1 Panax notoginseng seed Morphological features of seed T he colou r o f the s e e d s l l b e wh ite or yel lowi s h wh ite T he s e e d sh a l l b e hard-te xtu re, without any unusual smell or mildew 5.2.2 Moisture The moisture content in percentage mass shall not be less than 60 % © ISO 2017 – All rights reserved ISO 20408:2017(E) 5.2.3 Purity S e e d purity sha l l no t b e le s s tha n % 5.2.4 Viability Viabi l ity s l l no t b e le s s than % 5.2.5 Maturity M atu rity s l l no t b e le s s th an % 5.2.6 Fungi Neither Fusarium spp nor Alternaria spp shall be detected 5.2.7 000-seed weight, seed width, seed thickness and seed length T he 0 - s e e d weight, s e e d width, s e e d th ickne s s a nd s e e d leng th s l l comply with the re qui rements in Table Grade Table — Grading requirements of Panax notoginseng seed Seed width Seed thickness 000-seed weighta Firstb Secondc Thirdd Unqu a l i fie d g mm mm ≥10 ≥5,5 ≥5, 80 to