Thành phần hóa học và hoạt tính sinh học của cao chiết từ lá loài dương đồng bốc (adinandra bockiana e pritz ẽ diels)thành phần hóa học và hoạt tính sinh học của cao chiết từ l

Khoa hoc Y - Dude I Duoc hoc DOI: 10.31276/VJST.64( 12).34-38 Thanh phan hoa hocr va hoat tinh sinh hoc cua cao chiet tir la loai Diro’ng dong boc (Adinandra bockiana E Pritz ex x N g u y en H u u Q uan*, N g u y en T h | N g o c L a n , N g u y en T hj T h u N g a , S y D an h T h u tm g , C h u H o a n g M au K hoa Sinh hoc, T ruong D hoc S u p h a m Thai N guyen N g a y n h an bai /5 /2 ; n g a y c h u y e n p h an b ie n /5 /2 ; n g a y nhan p han b ie n 2 : n g a y ch ap nhan d a n g /7 /2 T om tat: D u o n g d o n g h o c c o te n k h o a h o c la A d in a n d b o ckia n a E P r it z e x D ie ls , t h u o c c h i D u o n g d o n g p h a n b o o’ t in h L a o C a i, V ie t N a m M o t so lo a i t h u o c c h i D u o n g d o n g d a d u o c n g h ie n c u u v e d a c d ie m t h u c v a t v a c a c c h a t c o h o a t tin h s in h h o c t r o n g c a y , tu y n h ie n c h o d e n n a y lo a i D u o n g d o n g h o c c h ir a d u o c ta c g ia n a o n g h ie n ciru D o d o n g h ie n c u u t h a n h p h a n h o a h o c v a h o a t tin h s in h h o c c u a c a o c h ie t tir la lo a i n a y la c o n g tr in h d a u tie n v a c a n t h ie t t r o n g th ir c tie n C a o c h ie t tir la c iia lo a i D u o n g d o n g h o c c o c h u a n h o m c h a t p o ly p h e n o l, ta n in v a c o u m a r in C a o e t h a n o l, d ic h lo r o m e t h a n v a e t h y l a c e t a t lie c h e s u p h a t t r ie n c u a v i k h u a n B su b tilis , L p la n ta r u m tin h k h u g o c t y d o D P P H m a n h , g ia tr j E C va S m a cescens n o n g d o , v a 0 p g /m l C a o e t h a n o l, d ic h l o r o m e t h a n e v a e t h y l a c e t a t e c o h o a t la n l u o t d a t , ,4 v a ,7 p g /m l C a o e t h a n o l c o h o a t tin h u c c h e c a c d o n g te b a o u n g th ir d a d a y , p h o i v a v u v o i g ia t r | I C 5(I la n l u o t la ,7 v a ,1 p g /m l K e t q u a n a y c h o th a y , lo a i D u o n g d o n g h o c la c a v tr o n g tie m n a n g b o i c h u a n h ie u h o p c h a t c o k h a n a n g k h a n g k h u a n , k h a n g o x y h o a v a u c c h e c a c d o n g te b a o u n g t h u Tir kh o a : c a o t o n g , c h o n g o x y h o a , D u o n g d o n g h o c k h a n g k h u a n , k h a n g te b a o u n g t h u C h i so p h a n loai: Oat van fle n g h ie n c u u n a y v a c u n g la n g h ie n c u u d a u t ie n v e lo a i D u o n g V ie t N a m la q u o c g ia c o d a d a n g s in h h o c p h o n g p h u v a tie m n a n g c a o v e c a y d u g c lie u T u y n h ie n , c o n h ie u lo a i c h u a d u g c n g h ie n c u u v e tarn q u a n tr g n g v a g ia tri la m th u o c c u a c h u n g , d a c b ie t la c a c lo a i th u o c c h i D u o n g d o n g V ie t N a m , c h i D u o n g d o n g c o k h o a n g lo a i, p h a n b o i r a c k h a p c a n u o c [1 ] , L o a i A d in a n d r a lie n ii d o n g b o c d e tra lo i c a u h o i tren N g h ie n c u u tie n h a n h ta c h c a o c h ie t , d jn h tin h th a n h p h a n h o a h g c c u a lo a i D u o n g d o n g b o c v a d a n h g ia h o a t tin h k h a n g k h u a n , c h o n g o x y h o a , k h a n g te b a o u n g th u c u a c a o c h ie t th u d u g c Vat lieu va pluffing phap nghien ciiti b u o c d a u d u g c n g h ie n c u n v e V a t lie u va m o i tn r o n g m id i c a y \ j tri p h a n b o d ia ly , d a c d ie m th irc v a t h p c v a tr in h t u g e n m a tK , v u n g IT S g iu p n h a n d ie n lo a i [2 , ] N g o a i ra, th a n h p h a n h o a h g c v a h o a t tin h k h a n g k h u a n tir la c u a lo a i A lie n ii d u g c c h ir n g m in h c o h o a t tin h k h a n g k h u a n , c h o n g o x y h o a v a r c c h e m o t s o d o n g te b a o u n g th u [4 ] , L a n T h i N g o c N g u y e n ■a c s ( 2 ) [5 ] d a n g h ie n c u u v e lo a i A m e g a p h y lla H u va n h a u t h a y c a o c h ie t tir la c u a lo a i n a y c o c h ii a c a c h g p c h a t r*: %n h e n o l, c o u m a r in ; d o n g t h o i c h u n g m in h c a o c h ie t tir la c o h : at tin h k h a n g k h u a n , k h u g o c t u d o v a u c c h e d o n g t e b a o u n g •J- _• • u d a d a y p h o i N h u v a y , c a c n g h ie n c u u a V ie t N a m h ie n * -;■ t i p :r u n g c h u y e u v a o A lie n ii v a A m e g a p h y lla la c a c lo a i L o a i D u o n g d o n g b o c s u d u n g tr o n g n g h ie n c u u d u g c th u ta i K h u b a o to n th ie n n h ie n H o a n g L ie n - V a n B a n t h u o c x a L ie r n P h u , h u y e n V a n B a n , tin h L a o C a i v a o th a n g / o d o c a o 0 m , tg a d o ° ’ ” N , ° ’2 ” E T e n k h o a h g c c u a lo a i d u g c x a c d jn h b o i P G S T S S y D a n h T h u b n g , T r u o n g D a i h o c S u p h a m , D a i h g c T h a i N g u y e n (h in h 1) L o a i D u o n g d o n g b o c th u th a p d u g c s u c h o p h e p c u a B a n q u a n ly K h u b a o to n th ie n n h ie n H o a n g L ie n - V a n B a n M a u la d u g c sir d u n g d e ta o c a o c h ie t v a n g h ie n c u u c a c b u o c tie p th e o - - i " be c h u y e u cr k h u v u c m ie n n u i p h ia B a c r d o n g b o c h a y c o n g o i la H o n g d a m T a m D a o , c o t - * o o a h v la ~ r :'" c ii ci A d in a n d b o c k ia n a E P ritz e x A d in a n d ) h g C h e ( T h e a c e a e ) r J r g o cao n ac r - g _-_ r.g A n e r«:c c rar -.: i i (A) c o n g trin h n g h ie n c u u n a o v e lo a i n a y V a y lo a i sf r u 2-6 m v a t h u o n g m o c tr e n s u o n n u i ang n oi c o d o cao 0 -1 0 m L oai D u o n g p h i n b o o T r u n g Q u o c v a V ie t N a m H ie n n a y , O ta u a g i a D ie ls , th u o c D uong dong j\ fcrr c h a t f ha> k h o n g c o h g p c h a t c o k h a n a n g k h a n g - a k h a n g te b a o u n g th u v a n e u c o th i la car rao E>a>- c h in h la c a u h o i d a t c h o 5*TZ> 12-2022 (B) Hinh Hinh anh cua loai Duong d6ng boc sir dung nghien ciru (A) Mat sau cua la; (B) Canh mang nu (anh d u g c chup beri nhom tac gia qua trinh thu mlu) 34 Khoa hoc Y - DitOc I D M c hoc lu p n g k h o n g d o i, s a u d o d e m n g h ie n n h o M a u n g h ie n d u p e Chemical composition and biological activity of leaf extracts of Adinandra bockiana E Pritz ex Diels n g a m c h ie t h a i la n b a n g e th a n o l tr o n g t h ie t b i s i e u a m n h ie t d o p h o n g D ic h t o n g s o d u p e c a t k ie t d u n g m o i d u d i ap su a t g ia m , n h ie t d o < ° C v a th u d u p e c a n c o e th a n o l C a n c o e th a n o l d u p e p h a lo a n g b a n g n u d e c a t, s a u d o c h ie t p h a n b o la n lu p t v d i c a c d u n g m o i la d ic h lo m e t h a n v a e th y l a c e ta t S a u k h i c a t d u o i d u n g Huu Quan Nguyen*, Thi Ngoc Lan Nguyen, Thi Thu Nga Nguyen, Danh Thuong Sy, Hoang Mau Chu m o i th u d u p e c a n d je h d ic h lo m e th a n , e t h y l a c e ta t v a c a n c h ie t C a c c a n d ic h th u d u p e s e s a y k h o d n h ie t d o ° C v a th u d u p e D ep artm ent o f Biology, Thai N guyen U niversity o f E ducation c a o s a y k h o t u o n g u n g C a c c a o c h ie t d u p e c h o v a o b in h th u y tin h , g h i n h a n , d a y k in v a b a o q u a n d n h ie t d o t h u d n g d e s u R e c e iv e d M a y 2 ; a c c e p te d J u ly 2 d u n g tr o n g c a c n g h ie n c u u tie p th e o Abstract: Dinh tinh cac hap chat co cao ethanol Adinandra bockiana E Pritz ex Diels collected in Lao Cai province, Vietnam belongs to the Adinandra genus Some species of the genus Adinandra have been studied for their botanical characteristics and bioactive compounds, however, A bockiana has not been studied to date Therefore, studying the chemical composition and biological activity of A bockiana leaf extracts is pioneering and necessary in reality The leaf extracts of A bockiana have been quantified and determined to contain tannins, coumarins and poiyphenolic compounds The ethanol extract, dichloromethane extract and ethyl acetate extract at the concentrations 20,60, and 200 pg/ml can inhibit the growth of B subtilis, L plantarum, and S marcescens The ethanol extract, dichloromethane extract and ethyl acetate extract have free radical reduction activity, which EC50values reached 0.2,0.47, and 5.7 jug/ml, respectively The ethanol extract exhibited inhibitory activities on gastric, lung, and breast cancer cell lines with IC50 values of 58.62, 48.73, and 43.15 pg/ml, respectively The results show that A bockiana is a potential plant that contains many compounds that have antibacterial, antioxidant, and anticancer activities D in h tinh p o ly p h e n o l: P h a n u n g v d i m u o i s a t (I I I ), la y m l c a o e th a n o l c h o v a o o n g n g h ie m k y h ie u la n lu p t la I v a II C h o th e m ,5 m l m u o i sa t (I I I ) v a o o n g II v a q u a n sa t h ie n tu p n g T u y t h e o s o lu p n g v a v i tri n h o m h y d r o x y l tr o n g p h a n t u p o ly p h e n o l m a c h o m a u lu c , x a n h h o a c n a u P h a n u n g v d i d u n g d ic h H , S d a c , la y m l c a o e th a n o l v a o o n g n g h ie m k y h ie u la n lu p t la I v a II C h o t h e m -2 g i o t H 2S d a c v a o o n g II v a q u a n sa t h ie n tu p n g K h i n h o H 2S 4d a c le n c a c d a n x u a t c u a fla v o n v a f la v o n o l th i c h o m a u v a n g d a m ; d o i v d i c h a lc o n v a a u r o n c h o m a u d o , d o th a m v a d o t u a i; fla v a n o n c h o m a u d o d a c a m d o s u c h u y e n th a n h c h a lc o n D in h tinh ca c c o u m a rin : L a y m l c a o e th a n o l v a o o n g n g h ie m k y h ie u la n lu p t la I v a II C h o v a o o n g II ,5 m l d u n g d ic h N a O H 10% D u n c a o n g tr e n b e p e a c h t h u y d e n s o i, la y d e n g u o i, sa u d o c h o th e m m l n u d e c a t v a o c a o n g I v a II Q u a n sa t th a y c h a t lo n g d o n g II ( c o k ie m ) trd n e n tr o n g s u o t h o a c tr o n g h o n o n g I ( k h o n g k ie m ) c o th e x e m la c o c o u m a r in N e u d e m a x it h o a o n g n g h ie m c o k ie m b a n g m o t v a i g i o t H C1 d a c m a la m c h o d ic h m a t m a u v a n g d u e h o a c x u a t h ie n k e t Keywords: Adinandra bockiana E Pritz ex Diels, antibacterial, tu a b o n g ( c u n g c o k h i x u a t h ie n k e t tu a ) th i c o th e k e t lu a n c o anti-cancer, antioxidant, total leaf extract c o u m a r in [ 6], D in h tin h tannin: Classification number: 3.4 L a y m l c a o e th a n o l v a o o n g n g h ie m k y h ie u la n lu p t la I v a II C h o t h e m th u d e th u v a n ilin /H 2S 4v a o o n g I la c d e u v a q u a n sa t N e u d ic h c h ie t c h u y e n s a n g m a u d o C a c lo a i v i k h u a n k ie m d in h L p la n ta n u m B su b tilis, S m a rce sc en s d a m la c h u n g to c o ta n in va Phieang phap thw hoat tmh khang khuan cua cao chiet d o K h o a S in h h o c , T r u o n g D a i h o c S u p h a m , D a i h o c T h a i N g u y e n c u n g c a p M o i tr u d n g L B d e n u o i c i y v i k h u in H o a t tm h k h a n g k h u a n c u a c a o c h ie t t u la c u a lo a i D u o n g k ie m d jn h c o th a n h p h a n g o m c a o n a m m e n ,5 % , N a C l ,0% , theo djnh d u p e n u o i h o a t h o a tr o n g m o i tr u d n g L B lo n g d ° C , la c 200 v o n g /p h u t tr o n g th d i g ia n 8h H u t p i d ic h n u o i m o i loai vi k h u a n d a d u p e h o a t h o a le n d ia m o i tr u d n g L B d a c va trai deu tr e n m a t th a c h c h o d e n k h i k h o D u e c a c g ie n g c o duemg kinh 10 m m tr e n d ia th a c h v a n h o 100 p i c a o c h ie t m o i lo a i tu la cua loai D u o n g d o n g b o c v a o g i e n g d c a c n o n g d o , 60 va 200 pg ml ( g i e n g d o i c h u n g b o s u n g D im e t h y l s u l f o x id e (DMSO) Datcac d ia p e tr i d a b o s u n g d ic h v a o tu la n h ° C khoang 1-2 gjo roi d |i v a o tu a m n u o i d ° C , t u 18 d e n gid Do duong kiah k h a n g k h u a n , c h u p h in h v a g h i la i ket qua Moi t h i n g h a e a id ta Q E p e p to n ,0 % M o i tr u d n g L B d a c b o s u n g ,0 % th a c h agar C a c d o n g t e b a o u n g th u g d m : M D A - M B - ( u n g th u v u ) , A G S (u n g th u d a d a y ) v a A (u n g th u p h o i) d u p e s u d u n g d e x a c d jn h h o a t tin h g a y d o c te b a o d o P h o n g T h u n g h ie m s in h h o c , V ie n C o n g n g h e S in h h o c , V ie n H a n la m K H & C N V ie t N a m c u n g cap Phvffng phap ctieu che mau thic hoat tinh L a c u a lo a i D u o n g d o n g h o c th u h a i d u p e r u a s a c h v a th a i n h o , p h o i tr o n g b o n g m a t , s a y k h o KHOA HOC CONG n h ie t d o ° C d e n k h o i 64(12) 12.2022 d o n g b o c d u p e x a c d in h b a n g p h u o n g p h a p d u e lo th a c h B M a h e s h v a S S a t is h ( 0 ) [7 ] C a c lo a i v i k h u a n k ie m 35 Khoa hoc Y - DiiOc I Dupe hoc la p la i la n D u o n g k ln h v o n g k h a n g k h u a n d u g c x a c d in h th e o C a c h a t D P P H E llip t ic in e D M S O , S R B d u g c c h u n g c o n g th u c: H = D - d (m m ) T r o n g d o : D la d u d n g k ln h v o n g to i sir d u n g la c h a t tin h k h ie t, d u g c c u n g c a p tir H a n g S ig m a k h a n g k h u a n (m m ); d la d u d n g k ln h g ie n g th a c h (m m ) A ld r ic h v a sir d u n g la m c h a t c h u a n , c u n g n h u d e p h a c a o c h ie t P h ir m g p h a p x a c d in h k h a n a n g c h o n g o x y h o a D P P H Ket qua K h a n a n g c h o n g o x y h o a c u a c a o c h ie t tir la c u a lo a i D u c m g d o n g h o c d u g c x a c d in h t h e o p h u o n g p h a p c u a J T ab art v a c s ( 0 ) [ 8] sir d u n g k h a n a n g tru n g h o a g o c tu d o D P P H 0 p i c a o c h ie t m o i lo a i d c a c n o n g d o ,5 , , D in h tin h c a c h o p c h a t co tr o n g la c u a lo a i D u o n g d o n g boc D in h tin h p o ly p h e n o l: 8, v a p g /m l d u g c C a o e th a n o l tir la c u a lo a i D u o n g b o s u n g ,9 m l d u n g d je h D P P H n o n g d o ,1 m M p h a tr o n g d o n g b o c d u g c d jn h tin h b a n g th u o c thir v d i m u o i sa t (I II), d u n g d ic h m e t h a n o l, la c d e u v a d e y e n tr o n g p h u t, sa u d o d o n h a n t h a y d u n g d ic h tr o n g o n g n g h ie m II c h u y e n s a n g m a u o b u d c s o n g n m K h a n a n g k h ir g o c t u d o D P P H d u p e tin h x a n h lu c v a k h a c s o v d i m a u c u a o n g n g h ie m I (h in h A ) th e o c o n g thvrc sau : K h i d in h tin h b a n g H , S d a c , d u n g d ic h tr o n g o n g n g h ie m K h a n a n g k h ir g o c t u d o D P P H (% ) = 0 x ( A c - A s) / A c II c h u y e n s a n g m a u n a u d a m h o n m a u c u a o n g n g h ie m I (h in h B ) N h u \ a y , tr o n g la c u a lo a i D u o n g d o n g b o c c o c h iia n h o m tr o n g d o : A c la d o h a p th u q u a n g c u a m a u d o i c h im g ; A s la d o h a p c h a t p o ly p h e n o l th u q u a n g c u a m a u c a n x a c d in h K h a n a n g c h o n g o x y h o a d u g c D in h tin h c a c tan n in : x a c d in h d u a v a o g ia tri E C 50 ( la n o n g d o m a u c o k h a n a n g k h u C a o e th a n o l tir la c u a lo a i D u o n g g o c t u d o D P P H d a t % ) d o n g b o c d u g c d jn h tin h b a n g th u o c thir v a n il in tr o n g H 2S P h ir m g p h a p x a c d in h tin h d o c te bao u n g th ir n u d i ca y d a n g d m lo p m a u c u a o n g n g h ie m I (h in h C ) N h u v a y , tr o n g la c u a lo a i T h u d o d o c te b a o u n g th u in vitro la m c h o d u n g d je h tr o n g o n g n g h ie m II c o m a u d o k h a c s o v d i D u o n g d o n g b o c c o c h u a n h o m c h a t ta n in d u g c x a c d jn h th e o D in h tin h c a c c o u m a rin : p h u o n g p h a p c u a A M o n k s v a c s ( 9 ) [9 ] C a o c h ie t tu la c u a lo a i D u o n g d o n g b o c d u g c c h u a n bj v a th u n g h ie m d n o n g d o 0 , , v a ,8 m g /m l C h a t th a m k h a o E llip t ic in e d c a c n o n g d o , , ,4 v a ,0 p g /m l d u g c sir d u n g n h u la c h a t d o i c h u n g d u o n g D M S O d n o n g d o 10% d u g c sir d u n g n h u la c h a t d o i c h u n g a m H a m l u g n g p r o te in t o n g s o c u a te b a o d u g c x a c K e t q u a h in h D n h a n th a y , k h i c h o ,5 m l N a O H 10% v a o o n g n g h ie m II, d e m d u n c a o n g n g h ie m tr e n b e p e a c h th u y , s a u k h i la m n g u o i v a c h o th e m m l n u d e c a t v a o c a h a i o n g th i d u n g d je h o n g II trd n e n tr o n g h o n S a u k h i c h o v a i g ig t d u n g d je h HC1 d a c v a o c a h a i o n g n g h ie m th i o n g II m a t m a u v a n g d u e ( h in h E ) T ir k e t q u a d jn h d in h d u a v a o m a t d o q u a n g h g c ( O D ) d o d u g c k h i th a n h p h a n tin h c h o th a y , tr o n g la c u a lo a i D u o n g d o n g b o c c o ch ira n h o m p r o te in c u a te b a o d u g c n h u d m b a n g S u lf o r h o d a m in e B ( S R B ) c o u m a r in C a c n h o m h g p c h a t p o ly p h e n o l , ta n n in v a c o u m a r in G ia tri O D d u g c d g c d b u d e s o n g 5 n m tren m a y E L I S A P la te c o y n g h la tr o n g v i e c irn g d u n g la m th u o c c h u a b e n h v a k e t R ea d er G ia tri O D m a y d o d u g c t y le th u a n v d i l u g n g S R B g a n q u a n a y la c o s o d e t ie n h a n h c a c n g h ie n c u u t ie p t h e o v e lo a i v d i p h a n t u p r o te in G ia trj O D c a n g ld n th i h a m l u g n g t e b a o v a D u o n g d on g boc h a m lu g n g p r o te in c a n g n h ie u K h a n a n g g a y d o c te b a o d u g c H o a t tin h k h a n g k h u a n c u a c a o c h ie t tir la c u a lo a i D ir m g d o n g bde x a c d in h b a n g n o n g d o c u a c h a t ire c h e % s u p h a t tr ie n cira te b a o N d n g d o u c c h e % te b a o (I C 50) d u g c x a c d in h la n o n g d o H o a t tin h k h a n g k h u a n c u a c a o e t h a n o l, e t h y l a c e ta t v a c u a m a u ire c h e d u g c % te b a o , c a c g o c t u d o h o a c e n z y m e C hat c h u a n d u g c c o i c o h o a t tin h to t k h i IC 50= p M [1 ] _s d ic h lo r o m e t h a n tir la c u a lo a i D u o n g d o n g b o c d u g c tr in h b a y (B) (C) (E) (D ) : n polyphenol (A, B), tannin (C) va coumarin (D, E) cao chiet ethanol tir la cua loai Diro’ng dong boc ■ (B) Phan irng vdi dung djeh H2S dac; (C) Phan ung vdi dung dich valin/H2S 4; (D) Phan img vdi NaOH; prr- —I ia-r W :ao ethanol toan ph§n ban dau; II: cao ethanol sau phan ung M(12) *2-2022 36 Khoa hoc Y - Duo'c I DUOc hoc la p la i la n D u o n g k ln h v o n g k h a n g k h u a n d u p e x a c d jn h th e o C a c h a t D P P H E llip t ic in e D M S O , S R B d u p e c h u n g c o n g th u c : H = D - d (m m ) T r o n g d o : D la d u d n g k ln h v o n g t o i sir d u n g la c h a t tin h k h ie t d u p e c u n g c a p tir H a n g S ig m a k h a n g k h u a n (m m ); d la d u d n g k ln h g ie n g th a c h (m m ) A ld r ic h v a sir d u n g la m c h a t c h u a n c u n g n h u d e p h a c a o c h ie t P h ic ffn g p h a p x a c d in h k lia n a n g c h o n g o x y h o a D P P H Ket qua K h a n a n g c h o n g o x y h o a c u a c a o c h ie t tir la c u a lo a i D u o n g d o n g h o c d u p e x a c d in h t h e o p h u o n g p h a p c u a J T ab art v a c s ( 0 ) [ 8] sir d u n g k h a n a n g tr u n g h o a g o c tu d o D P P H 0 p i c a o c h ie t m d i lo a i d c a c n o n g d o ,5 , , D in h tin h e d e h o p c h a t co tr o n g la c u a lo a i D u o n g d o n g boc D in h tin h p o ly p h e n o l: 8, v a p g /m l d u p e C a o e th a n o l tir la c u a lo a i D u o n g b o s u n g ,9 m l d u n g d ic h D P P H n o n g d o ,1 m M p h a tr o n g d o n g b o c d u p e d in h tin h b a n g th u o c th u v d i m u o i s a t (I II), d u n g d ic h m e t h a n o l, la c d e u v a d e y e n tr o n g p h u t, sa u d o d o n h a n t h a y d u n g d je h tr o n g o n g n g h ie m II c h u y e n s a n g m a u d b u d c s o n g n m K h a n a n g k h ir g o c tir d o D P P H d u p e tin h x a n h lu c v a k h a c s o v d i m a u c u a o n g n g h ie m I (h in h A ) th e o c o n g t h u c sau : K h i d in h tin h b a n g H 2S K h a n a n g k h ir g o c t u d o D P P H (% ) = 0 x ( A c - A s) / A c d a c , d u n g d ic h tr o n g o n g n g h ie m II c h u y e n s a n g m a u n a u d a m h a n m a u c u a o n g n g h ie m I (h in h B ) N h u v a y , tr o n g la c u a lo a i D u o n g d o n g b o c c o c h u a n h o m tr o n g d o : A c la d o h a p th u q u a n g c u a m a u d o i c h u n g ; A s la d o h a p c h a t p o ly p h e n o l th u q u a n g c u a m a u c a n x a c d in h K h a n a n g c h o n g o x y h o a d u p e D in h tin h c a c ta n n in : x a c d in h d ira v a o g ia tri E C 50 ( la n o n g d o m a u c o k h a n a n g khir C a o e th a n o l tir la c iia lo a i D u o n g d o n g b o c d u p e d jn h tin h b a n g th u o c thir v a n ilin tr o n g H , S g o c tir d o D P P H d a t % ) P h u c m g p h a p x a c d in h tin h d o c te b a o u n g th u ■n u d i cay d a n g d ffn l&p T h u d o d o c te b a o u n g th u in vitro la m c h o d u n g d je h tr o n g o n g n g h ie m II c o m a u d o k h a c s o v d i m a u c u a o n g n g h ie m I (h in h C ) N h u v a y , tr o n g la c u a lo a i D u o n g d o n g b o c c o ch ira n h o m c h a t ta n in d u p e x a c d jn h th e o p h u o n g p h a p c u a A M o n k s v a c s ( 9 ) [9 ] C a o c h ie t t u la c u a lo a i D u o n g d o n g b o c d u p e c h u a n b i v a th u n g h ie m d n o n g d o 0 , , v a ,8 m g /m l C h a t th a m k h a o E llip t ic in e d c a c n o n g d o , , ,4 v a ,0 p g /m l d u p e sir d u n g n h u la c h a t d o i ch irn g d u o n g D M S O d n o n g d o 10% d u p e s u d u n g n h u la c h a t d o i c h u n g a m H a m lu p n g p r o te in to n g s o c u a te b a o d u p e x a c D in h tin h c a c c o u m a rin : K e t q u a h in h D n h a n th a y , k h i c h o ,5 m l N a O H 10% v a o o n g n g h ie m II, d e m d u n c a o n g n g h ie m tr e n b e p e a c h th u y , sa u k h i la m n g u o i v a c h o th e m m l n u d e c a t v a o c a h a i o n g th i d u n g d je h o n g II tr d n e n tr o n g h o n S a u k h i c h o v a i g i o t d u n g d je h H C d a c v a o c a h a i o n g n g h ie m th i o n g II m a t m a u v a n g d u e (h in h E ) T ir k e t q u a d jn h d in h d u a v a o m a t d o q u a n g h o c ( O D ) d o d u p e k h i th a n h p h a n tin h c h o th a y , tr o n g la c u a lo a i D u o n g d o n g b o c c o c h u a n h o m p r o te in c u a te b a o d u p e n h u o m b a n g S u lf o r h o d a m in e B ( S R B ) c o u m a r in C a c n h o m h o p c h a t p o ly p h e n o l , ta n n in v a c o u m a r in G ia tri O D d u p e d o e d b u d c s o n g 5 n m tr e n m a y E L I S A P la te c o y n g h la tr o n g v i e c irn g d u n g la m th u o c c h u a b e n h v a k e t R ea d er G ia tri O D m a y d o d u p e ty le th u a n v d i lu p n g S R B g a n q u a n a y la c o s o d e tie n h a n h c a c n g h ie n c u u tie p t h e o v e lo a i v d i p h a n tir p r o te in G ia tri O D c a n g ld n th i h a m lu p n g te b a o v a D u o n g d o n g boc h a m lu p n g p r o te in c a n g n h ie u K h a n a n g g a y d o c te b a o d u p e H o a t tin h k h a n g k h u a n c u a c a o c h ie f tic la c u a lo a i D u o n g d o n g boc x a c d in h b a n g n o n g d o c u a c h a t ire c h e % sir p h a t tr ie n c u a te b a o N o n g d o tic c h e % te b a o (I C 50) d u p e x a c d jn h la n o n g d o H o a t tin h k h a n g k h u a n c u a c a o e th a n o l, e t h y l a c e ta t v a c u a m a u ire c h e d u p e % te b a o , c a c g o c t u d o h o a c e n z y m e d ic h lo r o m e t h a n tir la c u a lo a i D u o n g d o n g b o c d u p e trin h b a y C h a t c h u a n d u p e c o i c o h o a t tin h to t k h i IC 50= p M [1 ] ,A > (C) (D) (E) K e t aua dinh tinh polyphenol (A, B), tannin (C) va coumarin (D, E) cao chiet ethanol tip la cua loai Du’O’ng dong boc z- s r - - z E 5r j -'Z ? —-:i sat III: (B) Phan dng vdi dung dich H2S dac; (C) Phan dng vdi dung dich valin/H2S 4; (D) Phan dng vdi NaOH; y -»C dac: I cao ethanol toan phSn ban dSu; II: cao ethanol sau phan dng W -12) 12-2022 36 Khoa hoc Y - Duoc I DUOc hoc M o t s o h o p c h a t d i v d n g c h u a c o u m a r in c o lie n q u a n d e n c a c d a c L0I CAMON tin h n h u c h o n g v ie m [ ], k h a n g k h u a n [ ] , k h a n g v ir u s [ 14] v a c h o n g u n g th u [ ] N g o a i ra, c o u m a r in c u n g th e h ie n ta c d u n g g a y d o c te b a o u n g th u d o i v b i c a c t e b a o H e p ( l o a i te b a o b ie u N g h ie n ciru n a y d u p e tai trp b b i Q u y p h at trien K H & C N q u o c g ia (N A F O S T E D ) th o n g q u a d e tai m a so - 3 C ac ta c g ia x in ch a n th an h c a m o n m o n g ir b i), a n h h ir b n g tb i q u a trin h a p o p t o s is , sir b ie n m d t c u a lo p m a n g v i n h u n g m a o , la m g ia m h a m lu o n g h u y e t tucrng c u a TAI LIEUTHAMKHAO te b a o c h a t v a sir p h a n h u y n h a n [ ] C a o c h ie t t u la c u a lo a i [1] Pham Hoang Ho (1999), Publishing (A lie n ii) S u m lie n c o ch ira n h o m c h a t p o ly p h e n o l , f l a v o n o id v a c o u m a r in C a o e th a n o l, e t h y l a c U a t v a d ic h lo r o m e t h a n utc S ta p h y lo c o c c u s a u re u s, B su b tilis, S m a rce ssen s, S a rc in a lu tea , L p la n ta r u m v a E c o li a n o n g d o 0 p g /m l T r o n g d o , c a o d ic h lo r o m e t h a n c o c h e sir p h a t tr ie n c u a c a c lo a i v i k h u a n h o a t tin h k h a n g k h u a n t o t h o n s o v b i c a o e th a n o l v a e th y l a c e ta t [4 ] C a o c h ie t tir la c u a lo a i S u m la Io n (A m e g a p h y lla H u ) c h u a c a c h o p c h a t p o ly p h e n o l , c o u m a r in ; 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