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HG Precentered Fiber Illuminator (manual) (motorized) HG Precentered Fiber Illuminator Difficult lamp alignment is a thing of the past The Intensilight mercury-fiber illuminator employs a precentered, long-life lamp that requires no centering while allowing users to capture high-quality fluorescence images with uniform brightness C-HGFIE (motorized) Greatly increased brightness for green spectrum DC lighting for constant light intensity As DC (direct current) lighting is less influenced by frequency than AC (alternate current) lighting, DC provides constant, nonfluctuating light At wavelengths of around 450nm to 500nm, brightness is much higher than that of a conventional mercury lamp, making the lamp ideally suited to observation of green fluorescence such as FITC and GFP C-HGFI (manual) Spectrum comparison Typical AC lighting DC lighting 100.0 80.0 The use of a precentered lamp and dedicated optical fibers eliminates the need for cumbersome centering and focusing operations, even after the lamp is replaced Uniform brightness is always assured There are six levels of light intensity to choose from depending on the specimen The shutter allows light to the specimen to be easily shut off without the power being turned off Therefore, photobleaching can be reduced to a minimum when observing multi-stained specimens 70.0 Relative intensity Precentered lamp requires no alignment Shutter and light intensity control 60.0 Relative intensity Relative intensity 90.0 5 50.0 40.0 30.0 0.00 Time 80.00ms 0.00 20.0 10.0 Intensilight Mercury lamp 36 36 40 42 45 47 49 51 53 55 57 59 61 63 65 67 69 71 73 75 77 79 32 34 0.0 Wavelength (nm) Motorized model C-HGFIE available Off-center Precentered Light intensity control (manual) Shutter (manual) 2000-hour long-life lamp Safety measures The lamp lasts an average of 2000 hours, or 10 times longer than conventional mercury lamps Therefore, replacement costs and microscope down time are greatly reduced When the lamp replacement cover is open or the optical fibers are not attached, the interlock automatically shuts the light off to protect the user from possible light exposure Furthermore, when the lamp temperature sensor detects abnormally high temperatures, power is cut to protect the lamp The light intensity and shutter can be controlled from an optional dedicated remote controller or a PC that incorporates Nikon’s NIS-Elements imaging software The light intensity and shutter can be programmed for each application and controlled in conjunction with the microscope and peripheral equipment This enables automatic control during excitation light changeover and observation of multi-stained specimens Remote controller Reduced heat and electrical noise Dedicated optical fibers (1.5m, 3m) allow the light source to be placed away from the microscope, reducing heat and electrical noise on the microscope body This is particularly suited to long-time fluorescence observation (time-lapse observation) of live cells Configured with ECLIPSE TE2000-E Time 80.00ms Specifications Dimensional diagram Lamp Ultrahigh pressure 130W mercury lamp Lamp life Average 2000 hours ND (light intensity) (100%), (50%), (25%), (12%), 16 (6%), 32 (3%) Shutter response (Motorized) 100msec Power supply 100-240VAC 50/60Hz Dimensions (including protrusions) (Manual) 110 (W) x 307 (D) x 278.5 (H)mm (Motorized) 110 (W) x 296 (D) x 278.5 (H)mm Weight Approx 6kg Optical fiber length 1.5m/3.0m 31 Unit: mm 18 258 110 278.5 25.5 90 C-HGFI (manual) System diagram C-HGFI Intensilight (manual) Epi-fluorescence attachment for the TE2000 C-HGFIA HG100W adapter Epi-fluorescence attachment for TS100 C-HGFIF15 HG fiber 1.5m T1-HGFIA HG50W adapter C-LHGFI HG lamp Epi-fluorescence attachment for stereoscopic microscopes C-HGFIF-C HG controller P-FLA-HGFA adapter A Digital imaging head Universal epi-fluorescence attachment C-HGFIB HG100 adapter B Epi-fluorescence attachment for 50i/55i C-HGFIF30 HG fiber 3.0m Epi-fluorescence attachment for AZ100 PC C-HGFIE Intensilight (motorized) C-HGFA fiber adapter NIS-Elements imaging software Specifications and equipment are subject to change without any notice or obligation on the part of the manufacturer November 2006 ©2006 NIKON CORPORATION WARNING TO ENSURE CORRECT USAGE, READ THE CORRESPONDING MANUALS CAREFULLY BEFORE USING YOUR EQUIPMENT * Monitor images are simulated Company names and product names appearing in this brochure are their registered trademarks or trademarks NIKON CORPORATION Parale Mitsui Bldg., 8, Higashida-cho, Kawasaki-ku, Kawasaki, Kanagawa 210-0005, Japan phone: +81-44-223-2167 fax: +81-44-223-2182 http://www.nikon-instruments.jp/eng/ NIKON INSTRUMENTS INC 1300 Walt Whitman Road, Melville, N.Y 11747-3064, U.S.A phone: +1-631-547-8500; +1-800-52-NIKON (within the U.S.A.only) fax: +1-631-547-0306 http://www.nikonusa.com/ NIKON INSTRUMENTS EUROPE B.V P.O Box 222, 1170 AE Badhoevedorp, The Netherlands phone: +31-20-44-96-222 fax: +31-20-44-96-298 http://www.nikon-instruments.com/ NIKON INSTRUMENTS (SHANGHAI) CO., LTD CHINA phone: +86-21-5836-0050 fax: +86-21-5836-0030 (Beijing office) phone: +86-10-5869-2255 fax: +86-10-5869-2277 (Guangzhou office) phone: +86-20-3882-0552 fax: +86-20-3882-0580 NIKON SINGAPORE PTE LTD SINGAPORE phone: +65-6559-3618 fax: +65-6559-3668 NIKON UK LTD UNITED KINGDOM phone: +44-20-8541-4440 fax: +44-20-8541-4584 NIKON MALAYSIA SDN BHD MALAYSIA phone: +60-3-78763887 fax: +60-3-78763387 NIKON GMBH AUSTRIA AUSTRIA phone: +43-1-972-6111-00 fax: +43-1-972-6111-40 NIKON INSTRUMENTS KOREA CO., LTD KOREA phone: +82-2-2186-8410 fax: +82-2-555-4415 NIKON BELUX BELGIUM phone: +32-2-705-56-65 fax: +32-2-726-66-45 NIKON CANADA INC CANADA phone: +1-905-625-9910 fax: +1-905-625-0103 NIKON FRANCE S.A.S FRANCE phone: +33-1-45-16-45-16 fax: +33-1-45-16-00-33 NIKON GMBH GERMANY phone: +49-211-9414-0 fax: +49-211-9414-322 NIKON INSTRUMENTS S.p.A ITALY phone: +39-55-3009601 fax: +39-55-300993 NIKON AG SWITZERLAND phone: +41-43-277-2860 fax: +41-43-277-2861 Printed in Japan (0611-00)T Code No 2CE-OOOO-1 This brochure is printed on recycled paper made from 40% used material En