The present paper defines the optimal extraction window (OEW) for three-phase membrane-based liquidphase microextraction (MP-LPME) in terms of analyte polarity (log P), and anchors this to existing theories for equilibrium partitioning and kinetics. Using deep eutectic solvents (DES) as supported liquid membranes (SLM), we investigated how the OEW was affected by ionic-, hydrogen bond and π-π interactions between the SLM and analyte.
Journal of Chromatography A 1664 (2022) 462769 Contents lists available at ScienceDirect Journal of Chromatography A journal homepage: www.elsevier.com/locate/chroma Membrane-based liquid-phase microextraction of basic pharmaceuticals – A study on the optimal extraction window Maria Schüller a, Kim Tu Thi Tran a, Elisabeth Leere Øiestad a,b, Stig Pedersen-Bjergaard a,c,∗ a Department of Pharmacy, University of Oslo, P.O Box 1068 Blindern, 0316 Oslo, Norway Oslo University Hospital, Division of Laboratory Medicine, Department of Forensic Sciences, P.O Box 4459 Nydalen, 0424, Oslo, Norway c Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100 Copenhagen, Denmark b a r t i c l e i n f o Article history: Received July 2021 Revised 10 December 2021 Accepted 20 December 2021 Available online 23 December 2021 Keywords: Sample preparation Liquid-phase microextraction Hollow fiber Pharmaceuticals Deep eutectic solvents a b s t r a c t The present paper defines the optimal extraction window (OEW) for three-phase membrane-based liquidphase microextraction (MP-LPME) in terms of analyte polarity (log P), and anchors this to existing theories for equilibrium partitioning and kinetics Using deep eutectic solvents (DES) as supported liquid membranes (SLM), we investigated how the OEW was affected by ionic-, hydrogen bond and π -π interactions between the SLM and analyte Eleven basic model analytes in the range -0.4 < log P < 5.0 were extracted by MB-LPME in a 96-well format Extraction was performed from 250 μL standard solution in 25 mM phosphate buffer (pH 7.0) into 50 μL of 10 mM HCl acceptor solution (pH 2.0) with mixtures of coumarin, camphor, DL-menthol, and thymol, with and without the ionic carrier di(2-ethylhexyl) phosphate (DEHP), as the SLM The OEW with pure DES was in the range < log P < 5, and low SLM aromaticity was favorable for the extraction of non-polar analytes Here, extraction recoveries up to 98% were obtained Upon addition of DEHP to the SLMs, the OEW shifted to the range -0.5 < log P < 2, and a combination of 5% DEHP and moderate aromaticity resulted in extraction recoveries up to 80% for the polar analytes Extraction with ionic carrier was inefficient for the non-polar analytes, due to excessive trapping in the SLM The results from our study show that LPME performs optimally in a relatively narrow log P-window of ≈ 2–3 units and that the OEW is primarily affected by ionic carrier and aromaticity © 2021 The Author(s) Published by Elsevier B.V This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/) Introduction Microextraction techniques like liquid-phase microextraction (LPME) are popular choices for the extraction of targeted analytes from biological matrices Offering low consumption of organic solvents and the potential for automation, they are favorable with regards to greenness and efficiency in high-throughput applications [1] Current efforts focus on the implementation of LPME in routine laboratories, including the commercialization of equipment and better understanding of the optimal area of use This work will focus on the latter LPME can be performed with both two and three phases where the former is based on the partition of a substance between two immiscible liquids, like an aqueous sample and organic acceptor solution In a three-phase system, a second aqueous phase is introduced as the acceptor, allowing for the yield of cleaner extracts and better HPLC compatibility [2] The principle is based on liquid- ∗ Corresponding author E-mail address: stig.pedersen-bjergaard@farmasi.uio.no (S Pedersen-Bjergaard) liquid extraction with back extraction, with a configuration allowing the extraction to take place in a single step [3] Fig 1B illustrates the basic principle with two aqueous phases, the donor, and acceptor, separated by an immiscible organic supported liquid membrane (SLM) In a typical scheme for basic analytes, like many pharmaceutical drugs, the donor is alkaline, and the acceptor is acidic A high pH in the donor promotes the extraction of the analyte as a neutral species into the SLM, while a low pH in the acceptor promotes extraction and collection of the same analyte as the protonated species Extraction recovery will depend on the partition between these three phases [3] Extraction in a threephase system is typically used for organic analytes with weak base or acid properties An array of configurations for LPME has been developed through the years, each with specific advantages and limitations Beginning in 1996, pioneering work for two-phase LPME was conducted by Dasgupta [4] and Cantwell [5] with the introduction of single-drop microextraction (SDME), where a drop of organic liquid is immersed into an aqueous sample Another important milestone in the field of microextraction, pioneered by Pedersen-Bjergaard and Rasmussen, was the introduction of the https://doi.org/10.1016/j.chroma.2021.462769 0021-9673/© 2021 The Author(s) Published by Elsevier B.V This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/) M Schüller, K.T.T Tran, E.L Øiestad et al Journal of Chromatography A 1664 (2022) 462769 Fig A) Membrane-based liquid phase microextraction in 96-well format B) The principle of three-phase liquid-phase microextraction For the extraction of basic analytes the donor is alkalized to deprotonate the base The neutral analyte diffuses across the SLM and is protonated in the acidic acceptor solution membrane-based technique hollow-fiber liquid-phase microextraction (HF-LPME), which initiated the development of three-phase microextraction systems [6] In later years, several alternatives have been developed, including dispersive liquid-liquid microextraction (DLLME) [7], dispersive liquid-liquid microextraction based on solidified floating organic droplets (DLLME-SFO) [8], solvent bar microextraction (SBME) [9], and other membrane-based techniques such as membrane-based LPME (MB-LPME) in a 96-well format [10,11] and electromembrane extraction (EME) [12,13] In the present study, MB-LPME in a 96-well format was used (Fig 1A) LPME has been applied in various fields of health and life science, such as pharmacology [14], forensics [15], environmental chemistry [16], clinical chemistry [17], toxicology [18], and antidoping control [19] with scientific publications steadily increasing from the mid-’90s until today Although LPME has kept its relevance, implementation into routine laboratories has still not been facilitated One reason is the need for commercialized equipment, such as seen for solid-phase microextraction (SPME) While LPME equipment is still not commercially available, equipment for EME is very close to market, and when used without voltage, it operates as a MB-LPME system [20] Another reason is the need for generic methods, where molecular descriptors can be used to predict and select appropriate standardized extraction conditions for given analytes Although a large number of validated applications have been published [21–25], development of generic methods from this material is difficult Extractions have been done using very different experimental conditions, and performances have not been anchored sufficiently in fundamental understanding about partitioning and molecular interactions Therefore, in the present work we have looked into LPME again, now from a highly fundamental angle We have studied the extraction of selected basic pharmaceuticals as model analytes in the log P range from −0.4 to 5.0, using selected deep eutectic solvents as the SLM With the latter, hydrogen bonds, π -π -, and ionic interactions were controlled and varied systematically For each SLM, we investigated (1) which model analytes suffered from poor partition into the SLM, (2) which model analytes were extracted efficiently across the SLM, and (3) which model analytes suffered from poor partition from SLM and into the acceptor Model analytes belonging to (2) were within the optimal extraction window (OEW) of each SLM, and OEWs were assigned for different SLMs based on molecular interactions and analyte log P as a single molecular descriptor The purpose of this was to develop a starting point for the development of generic methods The work is therefore fundamental and general, and extractions were conducted only from pure standard solutions Applications, quantifications in biological fluids, method development, and validation, which are required to develop the final generic methods, will follow in future papers The intention is that the current paper will serve as fundamental reference Experimental 2.1 Chemicals and reagents Coumarin, thymol, camphor, DL-menthol, di(2-ethylhexyl) phosphate, hydrochloride acid 37%, sotalol hydrochloride, metaraminol bitartrate, atenolol, tyramine, ephedrine hydrochloride, metoprolol tartrate, pethidine hydrochloride, haloperidol, nortriptyline hydrochloride, loperamide hydrochloride, and methadone hydrochloride were purchased from Sigma-Aldrich (St Louis, MO, USA) Sodium hydroxide, acetonitrile (HPLC grade), and formic acid 99% (LC-MS grade) were purchased from VWR (Radnor, PA, USA) Deionized water was obtained with a Milipak® (0.22 μm filter) purification system from Milli-Q (Molsheim, France) Individual stock solutions of each analyte were prepared at concentrations of 1–4 mg mL−1 and dissolved in pure deionized water or 30% v/v methanol in deionized water From these, a polar analyte mix (sotalol, metaraminol, atenolol, tyramine, ephedrine, and metoprolol) and a non-polar analyte mix (pethidine, haloperidol, nortriptyline, loperamide, and methadone) were prepared with analyte concentrations of 10 μg mL−1 in 25 mM phosphate buffer These analyte mixes were pipetted into the donor wells The final methanol concentration never exceeded 0.3% and was not assumed to affect extraction recoveries To calculate the recovery, a 50 μg mL−1 standard polar mix and 50 μg mL−1 standard non-polar mix were prepared in 10 mM HCl All solutions were stored at °C and protected from light DES were prepared by weighing and mixing appropriate amounts of HBA components (coumarin or camphor) and HBD components (thymol or DL-menthol) in molar ratios of 1:1 and 1:2 The mixtures were heated in an oven (80 °C) for approximately 10 and vortexed until homogenous In SLMs with ionic carrier, DEHP was added to the DES mixtures in volume ratios of 0.5, 2, 5, or 10% 2.2 Liquid-phase microextraction procedure The equipment used is previously described in [22] The extraction was performed with a 96-well polypropylene donor plate with 0.5 mL wells from Agilent (Santa Clara, CA, USA) The acceptor plate was a 96-well MultiScreen-IP filter plate from Merck Millipore (Carrigtwohill, Ireland) The membrane material was polyvinylidene fluoride (PVDF) with a pore size of 0.45 μm A Platemax Pierceable Aluminum Sealing Film (Axygen, Union City, M Schüller, K.T.T Tran, E.L Øiestad et al Journal of Chromatography A 1664 (2022) 462769 CA, USA) was used to seal the acceptor plate Agitation during extraction was accomplished with a Vibramax 100 agitation system from Heidolph (Kellheim, Germany) The extraction was performed by pipetting 250 μL of standard solution into the well of the donor plate The filter on the acceptor plate was impregnated with μL of DES, creating the SLM Acceptor solution (50 μL) was pipetted into the wells of the acceptor plate The acceptor and donor plate were clamped together and sealed with adhesive foil The whole set-up was placed into the agitation device for extraction for 60 at 900 rpm After extraction, the donor and acceptor solution were collected and analyzed with HPLC-UV Details to the use of the extraction unit are provided in Supplementary Section 3.1 Extraction theory and definition of optimal extraction window The partition coefficient K1 between the donor and the SLM can be written as: K1 = K2 = K= Here, Ca, f inal and Cd,initial are the concentrations in the acceptor after the extraction and the concentration of analyte in the donor before the extraction, respectively The terms Va and Vd denote the volume of the acceptor and donor, respectively Table Physical-chemical properties of the studied drugs pKa −3.2 −2.5 −2.8 −2.0 −1.9 −1.5 −1.1 0.2 1.2 1.3 1.5 9.43 9.68 9.67 9.66 9.52 9.67 8.16 8.05 10.47 9.41 9.12 (5) ASLM DSLM K1 t Vd h (6) Here, Cd (t ) is the concentration in the donor as a function of time, Cd0 is the initial concentration in the donor (t = 0), ASLM is the surface area of the SLM, h is the thickness of the SLM, and DSLM is the diffusion coefficient of the analyte in the SLM Exact values of K1 and K2 are generally not available, unless 1octanol is used as the SLM With 1-octanol, computer-generated log P and log D values can be used to describe the efficiency of a three-phase LPME system K1 is equal to P for the neutral species, while K2 is set to 1/D for the species at pH Selecting three compounds with different log P and log D values from our set of model analytes, namely methadone, pethidine, and sotalol, the phase distribution for t = 60 was calculated based on Eq (5) and Eq (6) using computer-generated log P and log D values [28] Details of the calculations are found in Supplementary Section For methadone, log P is 5.0, while log D at pH is 1.5 The calculated distribution is illustrated in Fig The donor is depleted, 70% is trapped in the SLM, and only 30% of the analyte is extracted into the acceptor Because methadone is non-polar, K1 is large, and the donor is therefore rapidly depleted Correspondingly, K2 is small, causing methadone to suffer from serious membrane trapping Similar behavior is expected for other analytes with log P higher than 3.5, including loperamide and nortriptyline from our set of model analytes, with 1-octanol as the SLM After 60 min, the extraction system has entered equilibrium, and membrane trapping remains unaffected upon extension of the extraction time For sotalol, log P and log D are −0.4 and −3.2, respectively Sotalol is a polar substance; K1 is low, while K2 is high Therefore, partition into the SLM is highly unfavorable, and mass transfer is correspondingly slow After 60 of extraction, 99% of sotalol is Eleven drug compounds were selected as model analytes with −0.4 < log P < 5.0 This range was chosen, as it represents a common range for small-molecule drugs [26] Polar analytes were defined as having log P < 2.0 and non-polar analytes were defined as having log P > 2.0 Analyte log P, log D at pH 2, and pKa values are shown in Table Log D (pH 2) K × Va × 100% K × Va + K1 × VSLM + Vd Cd (t ) = Cd0 · exp − Results and discussion −0.4 −0.1 0.4 0.7 1.3 1.8 2.5 3.7 4.4 4.7 5.0 (4) Here, Va , Vd , and VSLM denote the volumes of the acceptor, donor, and SLM, respectively Eq (4) is valid for estimating extraction recoveries when the system has entered equilibrium The time required to reach equilibrium is compound-dependent Previous work has shown that partition into the SLM often is the rate-limiting factor in threephase LPME [27] Thus, kinetics are controlled by K1 , and can be modelled using the following equation: (1) Log P Ceq,a = K1 × K2 Ceq,d R (% ) = The extraction recovery was calculated by Eq (1): Sotalol Metaraminol Atenolol Tyramine Ephedrine Metoprolol Pethidine Haloperidol Nortriptyline Loperamide Methadone (3) Assuming that K is unaffected by the organic phase, K2 is decreasing when K1 is increasing, and vice versa Based on the partition coefficients, the theoretical extraction recovery in three-phase LPME may be calculated by: 2.4 Calculations Drug compound Ceq,a Ceq,SLM Here, Ceq,a is the equilibrium concentration in the acceptor The overall partition coefficient K between the donor and acceptor can be expressed as the product of K1 and K2 : HPLC-UV analysis was performed on a 30 0 Ultimate HPLCUV (Thermo Fisher Scientific, Waltham, MA, USA) with an Acquity UPLC HSS T3 (2.1 mm I.D × 150 mm, 1.8 μm particle size) purchased from Waters (Wexford, Ireland) Mobile phase A consisted of 0.1% formic acid in 95:5 deionized water/methanol (v/v) Mobile phase B consisted of 0.1% formic acid in 95:5 methanol/deionized water (v/v) Further details on the elution gradients, detection parameters and chromatograms are provided in Supplementary Section Ca, f inal Va × × 100% Cd,initial Vd (2) Here, Ceq,d and Ceq,SLM are the equilibrium concentrations in the donor and SLM, respectively Correspondingly, the partition coefficient K2 between the SLM and acceptor can be written as: 2.3 High performance liquid chromatography with UV-detection R= Ceq,SLM Ceq,d Chemicalize was used to generate structure properties (retrieved 27.02.2021, https://chemicalize.com) developed by ChemAxon (https://www.chemaxon com) [28] M Schüller, K.T.T Tran, E.L Øiestad et al Journal of Chromatography A 1664 (2022) 462769 (aromatic ring count) Mixtures of coumarin and DL-menthol did not form stable deep eutectic mixtures, seen as precipitation after heating, and were therefore not tested DES compositions with or without ionic carrier were selected based on literature and previous experience [31–33] 3.3 Pure eutectic solvents In the first set of experiments, the effect of hydrogen bond interactions was investigated using SLMs with different HBA/HBD ratios and an aromatic ring count equal to zero Two SLMs were compared, namely camphor:DL-menthol in molar ratios 1:1 and 1:2 (HBA/HBD ratio 2.0 and 1.5, respectively) To establish mass balance data, both the acceptor and the donor were analyzed Experimental data obtained with camphor:DL-menthol (1:1) are summarized in Fig 3A The results were in accordance with the theoretical discussion in Section 3.1 The polar model analytes sotalol, metaraminol, atenolol, tyramine, ephedrine, metoprolol, and pethidine largely remained in the donor These compounds, representing the log P range from −0.4 to 2.5, suffered from slow kinetics For haloperidol, nortriptyline, loperamide, and methadone, with log P values between 3.7 and 5.0, recoveries were generally high, and this indicated that the extraction was under ideal or near-ideal conditions in the OEW When the molar ratio of camphor and DL-menthol was changed from 1:1 to 1:2 (results shown in Fig 3B), the HBA/HBD ratio decreased from 2.0 to 1.5 The OEW was seemingly unaffected, but the overall membrane trapping increased for the model analytes within this region Since the aromatic ring count was zero with both SLMs, the slight increase in membrane trapping was caused by increased HBD activity In a next set of experiments, the effect of aromatic ring count was investigated Three different SLMs were compared, namely coumarin:thymol, camphor:thymol, and camphor:DL-menthol, all in molar ratios of 1:1 These SLMs represented three, one, and zero aromatic ring counts, respectively, while the HBA/HBD ratio was 2.0 in all cases Experimental data obtained with coumarin:thymol (1:1) are summarized in Fig 3C This SLM was highly aromatic, with a ring count of three Sotalol, metaraminol, atenolol, tyramine, ephedrine, and metoprolol (−0.4 < log P < 1.8) were prone to slow kinetics Pethidine (log P = 2.5), however, was extracted with 65% recovery and was within the OEW for this SLM For haloperidol, nortriptyline, loperamide, and methadone, with log P between 3.7 and 5.0, mass balance data verified significant membrane trapping in the range between 64 and 100% With camphor:thymol (1:1) (Fig 3D), the aromatic ring count was one Again, the polar analytes in the range −0.4 < log P < 1.8 suffered from slow kinetics and were not extracted from the donor Here, the OEW was shifted towards lower log P values compared to previously discussed SLMs The analytes in the log P range from 2.5 to 5.0 were extracted to some extent, but membrane trapping still dominated This was especially evident for loperamide, possibly due to strong π -π interactions between the SLM (ring count one) and loperamide (ring count three) With camphor:DL-menthol (1:1), the SLM was non-aromatic Pethidine was no longer within the OEW, while haloperidol, nortriptyline, loperamide, and methadone were all extracted with high recoveries (Fig 3A) Since all the non-polar model analytes are aromatic, the change to a non-aromatic SLM increased their extraction into the acceptor Therefore, the optimal extraction window was shifted slightly towards higher log P Loperamide was now extracted with high recovery, most probably because the π -π interactions were absent No significant membrane trapping was observed for this SLM, but is assumed to be present above the tested log P range Fig Calculated relative amounts of sotalol, pethidine, and methadone found in the donor (blue), SLM (yellow), and recovered in the acceptor (green) with t = 60 still left in the donor, and extraction is limited by slow kinetics With 1-octanol as the SLM, slow kinetics is to be expected for all substances with log P below 2.0 Recoveries can be improved by increasing extraction time, and theoretically, exhaustive extraction is achieved at the end However, this requires extraction far beyond 60 min, which is of little relevance for analytical applications For pethidine, log P and log D are 2.5 and −1.0, respectively Compared to methadone, K1 is lower and K2 is higher Due to this, the balance between K1 and K2 is more appropriate, and the equilibrium distribution is much more in favor of high recovery for pethidine (Fig 2) With K1 sufficiently high to prevent slow kinetics, without causing membrane trapping, the analytes are extracted under ideal conditions With 1-octanol as the SLM, similar behavior is expected for other analytes with log P in the range of 2.0 to 3.5 This is the optimal extraction window (OEW) for 1-octanol, where, in theory, exhaustive extraction is to be expected Although the partition coefficients change if 1-octanol is replaced by another solvent, the principles discussed above are still valid The here described theoretical trends have also been observed in previous literature [29,30] Thus, a given SLM solvent has an OEW, where K1 and K2 are balanced and exhaustive extraction can be expected More polar analytes (low K1 ) are expected to suffer from slow kinetics, while more non-polar analytes (low K2 ) are prone to membrane trapping In the following, OEWs were established for different SLMs, with reference to analyte log P 3.2 Tuning SLM properties with deep eutectic solvents OEWs were investigated with different deep eutectic solvents (DES), also including combinations with DEHP as the ionic carrier This enabled individual assessment of hydrogen bond-, π -π , and ionic interactions, and their impact on the OEW Four eutectic components, two HBA components, and two HBD components were mixed in molar ratios of 1:1 and 1:2 Coumarin and camphor were selected as HBA components, while thymol and DLmenthol were selected as HBD components Coumarin and thymol, provided aromatic character to the deep eutectic solvents Table gives an overview of the tested membrane compositions, including the number of HBA and HBD sites, and aromatic properties M Schüller, K.T.T Tran, E.L Øiestad et al Journal of Chromatography A 1664 (2022) 462769 Fig Relative amounts of model analyte found in the donor (blue), SLM (yellow), and recovered in the acceptor (green) after 60 of extraction for A) camphor:DLmenthol (1:1) and B) camphor:DL-menthol (1:2), C) coumarin:thymol (1:1), D) camphor:thymol (1:1) The error bars represent the standard deviation (SD) of the acceptor with n = mine, and ephedrine and the non-polar analytes haloperidol, nortriptyline, and loperamide were all extracted with low recoveries ( 60%), were measured for the non-polar analytes pethidine and methadone The data show no apparent correlation with analyte log P values When increasing the DEHP percentage to 2% (See 3.4 Eutectic solvents with DEHP In the next set of experiments, the effect of ionic interactions was investigated by adding 0.5, 2, 5, and 10% DEHP to the nonaromatic SLM camphor:DL-menthol (1:1) Experimental data obtained with 0.5% DEHP are summarized in Fig 4A The polar analytes sotalol, metaraminol, atenolol, tyra5 M Schüller, K.T.T Tran, E.L Øiestad et al Journal of Chromatography A 1664 (2022) 462769 Fig Relative amounts of model analyte found in the donor (blue), SLM (yellow), and recovered in the acceptor (green) after 60 of extraction for A) camphor:DLmenthol (1:1) + 0.5% DEHP, B) camphor:DL-menthol (1:1) + 2% DEHP, C) camphor:DL-menthol (1:1) + 5% DEHP, and D) camphor:DL-menthol (1:1) + 10% DEHP, E) camphor:DL-menthol (1:1) + 5% DEHP, F) camphor:thymol (1:1) + 5% DEHP, and G) coumarin:thymol (1:1) + 5% DEHP Sample size n = The error bars represent the standard deviation (SD) of the acceptor with n = M Schüller, K.T.T Tran, E.L Øiestad et al Journal of Chromatography A 1664 (2022) 462769 Table Overview of tested membranes, including computer-generated HBA sites, HBD sites, aromatic ring count, and molecular structures of the eutectic components SLM composition HBA sitesa HBD sitesa Aromatic ring counta Ionic carrier (Y/N) Coumarin:thymol (1:1) Camphor:thymol (1:1) Camphor: DL-menthol (1:1) Camphor: DL-menthol (1:2) Camphor: DL-menthol (1:1) + 0.5% DEHP Camphor: DL-menthol (1:1) + 2% DEHP Camphor: DL-menthol (1:1) + 5% DEHP Coumarin:thymol (1:1) + 5% DEHP Camphor:thymol (1:1) + 5% DEHP Camphor: DL-menthol (1:1) + 10% DEHP 2 2 2 1 1 1 0 0 N N N N Y Y Y Y Y Y Coumarin Camphor DL-menthol Thymol DEHP a Chemicalize was used to generate structure properties (assessed by 01.2020, https://chemicalize.com) developed by ChemAxon (https://www.chemaxon.com) [28] est extraction recoveries were obtained for camphor:thymol + 5% DEHP (Fig 4F) This SLM has an aromatic ring count equal to one Further increasing or decreasing the aromatic ring count increased the amount of membrane trapping This SLM was the overall best for the polar analytes For the non-polar analytes, the increase in aromatic ring count decreased the extraction recoveries due to membrane trapping This shows that aromatic SLMs in combination with DEHP are highly unfavorable for the extraction of nonpolar analytes Fig 4B), the extraction recoveries of the polar analytes generally increased The increase was both due to increased donor depletion and decreased membrane trapping Extraction recoveries all decreased for the non-polar analytes From the mass balance data, it is evident that this was due to an increase in membrane trapping For higher DEPH concentrations, namely and 10%, similar observations were made (Fig 4C and 4D) The extraction recoveries of the polar analytes were around 50%, except for atenolol, which suffered from membrane trapping For the majority of the non-polar analytes, the recoveries were below 10%, and mass balance data revealed serious membrane trapping Pethidine was extracted close to ideal conditions when DEPH concentrations were high, while the extraction recovery of methadone fluctuated with the higher DEPH concentrations From the obtained mass balance data, addition of DEHP generally increased the extraction of polar analytes from 5- to a 50%level This shows that ionic interactions are essential for the extraction of these analytes, even with deep eutectic solvents DEHP increases the partition into the SLM, and the donor is depleted For the non-polar analytes, except pethidine and methadone, the addition of an ionic carrier significantly increased the amount of membrane trapping For nortriptyline, as an example, membrane trapping increased from 20 to 93% upon addition of 10% DEHP It is suspected that the ion exchange at the membrane-acceptor interface is too weak to accommodate for the strong ionic and hydrophobic interactions between the non-polar analytes and the SLM Pethidine and methadone are behaving similarly to the polar analytes, where higher DEHP concentrations increased donor depletion In the last set of experiments, the combined effect of aromaticity and ionic interactions were investigated Hydrogen bonding was not investigated further, as previous experiments revealed an insignificant effect on the OEW For this last set of experiments, 5% DEHP was added to coumarin:thymol, camphor:thymol, and camphor:DL-menthol, all in molar ratios of 1:1 These SLMs represented zero, one, and three aromatic ring counts, respectively; while the HBA/HBD ratio was equal to 2.0 in all SLMs Mass balance data are shown in Fig For the polar analytes, the high- Conclusion In the present study, we proposed the terms optimal extraction window (OEW), slow kinetics, and membrane trapping, to express the log P range where a given three-phase membranebased liquid-phase microextraction (MB-LPME) system can be expected to be optimal We investigated a selection of supported liquid membranes (SLM) based on deep eutectic solvents (DES) with varying HBA/HBD and aromatic ring count, and established their OEWs using a set of pharmaceutical drugs (−0.4 < log P < 5.0) as model analytes With pure DES, extraction was primarily facilitated by hydrogen bond and π -π interactions, and the OEWs were typically within the range < log P < Model analytes with log P < ∼2 suffered from slow kinetics, while model analytes with log P > ∼3.5 were prone to membrane trapping OEWs were slightly affected by the HBA/HBD ratio and shifted towards higher log P values with increasing aromatic ring count Within the OEWs, the model analytes were extracted with high recoveries, except for highly aromatic ones, which were prone to strong π -π interactions and membrane trapping Although the pure DES were strong solvents regarding hydrogen bond and π -π interactions, they were insufficient for the extraction of polar analytes (log P < 2) With the addition of ionic carrier (DEHP) to the SLMs, polar analytes were efficiently extracted and the OEWs shifted to the range −0.5 < log P < The SLM with 5% DEHP and moderate aromaticity resulted in extraction recoveries of up to 80% for the polar analytes For the non-polar analytes, however, the same SLM suffered from membrane trapping The results from our study show that a given three-phase liquid-phase microextraction system is efficient only M Schüller, K.T.T Tran, E.L Øiestad et al Journal of Chromatography A 1664 (2022) 462769 in a relatively narrow log P range within the optimal extraction window (OEW) Minor shifts of the OEW can be expected from alterations of HBA, HBD, and aromatic ring count, while major shifts can be expected when introducing ionic carriers Identifying OEWs for different SLMs is a step towards a better understanding of LPME In the future, this can help users in routine laboratories to define the possibilities and limitations of a given LPME system More research on SLMs with an added carrier is planned to increase knowledge on carrier-mediated LPME This will help developing better 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Tran, E.L Øiestad et al Journal of Chromatography A 1664 (2022) 462769 CA, USA) was used to seal the acceptor plate Agitation during extraction was accomplished with a Vibramax 100 agitation