grass carp ctenopharyngodon idellus invariant chain of the mhc class ii chaperone protein associates with the class i molecule

Accepted Manuscript Grass carp (Ctenopharyngodon idellus) invariant chain of the MHC class II chaperone protein associates with the class I molecule Fang-fang Chen, Hai-bin Lin, Jin-chun Li, Yong Wang, Juan Li, Da-gan Zhang, Wei-yi Yu PII: S1050-4648(17)30043-8 DOI: 10.1016/j.fsi.2017.01.030 Reference: YFSIM 4407 To appear in: Fish and Shellfish Immunology Received Date: 26 September 2016 Revised Date: 22 December 2016 Accepted Date: 20 January 2017 Please cite this article as: Chen F-f, Lin H-b, Li J-c, Wang Y, Li J, Zhang D-g, Yu W-y, Grass carp (Ctenopharyngodon idellus) invariant chain of the MHC class II chaperone protein associates with the class I molecule, Fish and Shellfish Immunology (2017), doi: 10.1016/j.fsi.2017.01.030 This is a PDF file of an unedited manuscript that has been accepted for publication As a service to our customers we are providing this early version of the manuscript The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain ACCEPTED MANUSCRIPT Grass carp (Ctenopharyngodon idellus) invariant chain of the MHC class II chaperone protein associates with the class I molecule Fang-fang Chen, Hai-bin Lin, Jin-chun Li, Yong Wang, Juan Li, Da-gan Zhang, Wei-yi Yu Key Laboratory of Zoonoses of Anhui Province, Anhui Agricultural University, Hefei Anhui,230036, China RI PT Corresponding author AC C EP TE D M AN U SC E-mail: yuwy@ahau.edu.cn ACCEPTED MANUSCRIPT ABSTRACT RI PT Grass carp (Ctenopharyngodon idellus) invariant chain of the MHC class II chaperone protein associates with the class I molecule The invariant chain (Ii) is an important immune molecule, as it assists major histocompatibility complex (MHC) class II molecules to present antigenic peptides The relationship between the Ii and MHC molecules in teleosts remains poorly understood This study focused on the molecular structure of grass carp Ii (gIi), its organ distribution, correlations with gene transcription, and the association with MHC gIi cDNA was cloned using designed degenerate primers and the rapid amplification of cDNA ends method (RACE) The gIi sequence was 92%–96% similar to that of other teleosts, but only 52%–67% similar to that of mammals, respectively The gIi gene was distributed in all 12 organs examined by PCR The gIi gene transcription levels were markedly higher in organs enriched with immune cells than in other organs (P