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Nghien cim Phdt triin yd tine dune Cone nehe Vdt lieu (KC 02/06 10) Ha Ndi 27/11/2009 N G H I E N C U U C H E T A O T H I E T B I D O C H O C A M B I E N S I N H H O C t f N G D U N G T R O N G P H A[.]
Nghien cim Phdt triin yd tine dune Cone nehe Vdt lieu (KC.02/06-10)- Ha Ndi 27/11/2009 NGHIEN C U U CHE TAO THIET B I DO CHO C A MB I E N SINH HOC: t f N G DUNG TRONG PHAN TICH M O I TRUONG Mai Anh Tuan , Vu Quang Khue va Nguyen Due Chien Wien Dao tao Qudc te ve Khoa hoc Vdt lieu (ITIMS) Vien Vat ly Ky thuat Trucmg dai hoc Bach Khoa Hd Ndi, So 1, Dai Cd Viet, Hd Ndi Email: mtuan@itims.edu.vn 1 2 Tom tat Bai bdo mo td viec phdt trien thiet bi xdeh tay de phdt hien du luong thudc tru sau nude Chiing tdi sir dung chip PsoC CY8C24894 va cdc chip Max 038, LMC 6482, pA 741 di cai thien ty sd tin hieu/dn ciia mach Thiit bi duac thiit ki di lam viec vdi cam biin dd cam bien duac sic dung nhu dien cue lam viec, cam bien lai cd vai tro nhu phdn so sanh nhdm giam thieu nhieu (on) mdi trudng gay Thiit bi duac thiit ki di cd thi phdt xung, duac tich hap san, vdi tdn sd cd thi thay ddi (10-30 kHz) vd bien thay ddi (100 mv - 300 mV) Xung hinh Sin se quet tren cap dien cue lam viec Tin hieu Idi duac tinh bang sut thi tren hai dien trd KQ Phuang phdp phdt hien dua tren tuong tdc sinh hda giua chdt va chdt dich gay su thay ddi tinh chdt sinh/hda tren dien tich nhay ciia cam biin Bd phdn chuyen ddi se lam nhiem vu chuyin nhung thdng tin dd cdc tin hieu dien, duac thu thap, xu ly va hiin thi tren man hinh tinh thi long Thiit bi duac img dung cho cdc cam bien sinh hoc tren ca sd enzyme de phdt hien cdc chdt gay d nhiem nude I GIOI THIEU Chung ta clang phai doi mat voi mot thuc te la su gia tang nhanh chong cua cac loai hop chat nhiem d dang vo co va huu co gay ton hai Ion toi sue khde cua ngudi Thuc te dang d | t cho cac nha khoa hoc phai phat tridn cac ky thuat phan tich nhanh, hieu qua va re tien dd thuc thi cac chuong trinh nghien c i m m d i trudng mang tinh tdng thd [1-4] Cam bien sinh hoc duoc coi la mot nhung cong cu bd xung cac phuong phap phan tich truyen thdng k y thuat cd thd dap ung cac yeu cau khat khe ve dd chinh xac, dd lap lai, kha nang phan tich nhanh, kich thudc nhd va gia [5-7] M o t he thdng phan tich sir dung cam bien sinh hoc thudng bao gdm bd phan cam nhan, bd phan chuyen ddi va bd phan thu thajj xir l y sd lieu Viec phat trien, tdi uu hda cac qua trinh, linh kien hoac thiet b i m d i phan deu cd gia t r i to l d n gdp phan d i y nhanh viec dua cac he thdng phan tich diing cam bidn sinh hoc vao ung dung rdng rai cudc sdng Y tudng xay dung va phat tridn mot bd thiet b i phan tich cam tay, cd kha nang hoat ddng ddc lap, sir dung cho cam bien dd dan v d i dd t i n cay cao, lap lai tdt cd kha nang thay thd cac thiet bi thuong mai dang duoc sir dung, cu thd la L o c k - I n A m p l i f i e r SR830 ciia M y Thiet bi can phat duoc cac xung sin v d i tan sd, bidn cd the thay ddi duoc, cd kha nang giao tiep de dang v d i may tinh thdng qua cac cong giao tiep thdng dung II THUC NGHIEM Xay dung so khdi cho thiet bi Dau vao cam bidn can duoc quet mot t i n hieu xoay chidu v d i tan sd 10 K H z va bidn dd 100 m V T i n hieu duoc quet tren hai chan ciia hai cam bien, dd mot ddng vai trd la dien cue hoat ddng, mot ddng vai trd la dien cue so sanh T i n hieu l o i duoc tinh la su chenh the gitra hai dien cue r o i tren hai dien tia KQ Dua tren cac yeu cau ciia mach nguyen ly chung tdi xay dung so dd khdi cho thidt b i , so dd Theo dd, t i n hieu hinh sin se duoc phat tir khdi phat tin hieu, di vao khdi cam bien, khdi tach sdng sau duoc dua qua bd tuong tusd ( A D C ) , trir dien ap va di vao khdi xir l y trung tam Ngoai nhung chi tidt nay, mot sd tinh nang khac duoc tich hop vao thidt b i qua trinh phat tridn 245 : ::r.i F " • a l\g CO H dims Cons nshe Yd: lieu iKC.02/06-10)- XlLTf?UN3!AJ,1 r v / tfo Khoi phat xung su dung chip Max 038 cho dang song sine dua vao dien cue cua cam bien co dai lam viec ve bien tir 100 -r 300 m V tan so 10 -r 30 k H z v o i muc dich co the cap t i n hieu cho nhieu loai cam bien dien hoa khac K h o i t i c h sdng bien duoc thuc hien b d i chip L M C 6482 thuc hien chinh luu tin hieu nho v d i dien ap nhd nhat 10 m V [10] T i n hieu sau k h i qua bd chinh l u u duoc dua tdi chip CY8C24894, chip ho SoC cho phep vide lay m l u ddng thoi cam bien va tir cam bien L M 335 de xac dinh nhiet m d i trudng D d phan giai k h i lay m l u len tdi 1.2 m V [10] N h i e m vu ciia chip la chuyen ddi t i n hien tir cam bien sang dang so va hien thi tin hieu len man hinh tinh the long m KET QUA Cdc dac tuyen tdn so vd bien dp cud thiet bi Sau mot then gian nghien cuu thiet ke mach nguyen ly va tien hanh che tao mach tich hop chiing toi thu duac bo mach chinh nhu trons hinh Hinh Bo mach chinh cua thiet bi bao gom cdc khoi nhu ban ve so dd khdi Thiet bi co khd ndng kit ndi thdng qua cong RS232 hoac USB 2-r Nghien ciru Phdt va Uhg d u n s C n g n g h i V d t l i i u ( C-02/06-10)K Ha Ndi 27/11/2009 Tren may hien song (hinh 2) cac xung hinh Sin loi ciia thiet bi (loi vao cam bien) co t i n sd va bien clo nhu yeu c i u ban dau dat K h o i phat tin hieu hinh Sin dua vao dien cue cam b i l n co gia tri dien ap 150 m V + m V ; 15 k H z ± k H z v o i budc nhay; d gia t r i bien va t i n sd dd tin hieu cho ket qua phan tich tren may hien sdng cd dd chuan net va it chiu anh hudng ciia tin hieu dien l u d i xoay chidu V d i thang bien dd va tan sd dua vao cam bien khac nhau, bd phat tin hieu ciia thidt bi cho phep thuc hien ghep ndi v d i nhieu bd kit ciia cam bien sinh hoc dien hda va cd the sir dung phan tir chuyen ddi la enzim hoac A D N k h i chirc nang hda be mat Hinh Tin hieu xung hinh Sin loi vao cam bien Dd thuc hien lay tin hieu mot chieu tir kenh cua cam bidn mien dich, khdi tach sdng bidn dd duoc xay dung v d i 05 L M C Sau tin hieu (hinh 2) duoc dua qua 05 bd loc RC trudc k h i dua vao chip xir l y Gia t r i dien ap tir cam bidn nam khoang 10 -r 50 m V Ket qua hien thi cho thay tin hieu khdng bi anh hudng ciia nhieu ben ngoai va cd dd sut ap tren linh kien 10 % Hinh Tin hieu V^cua bo tdch song nhay pha Didu cho phep thidt b i tach sdng tin hieu cd the dat t d i 10 m V V d i vide sir dung bd phat tin hieu dua vao cam bien la M a x 038 va bd tach sdng nhay pha L M C 6482 thiet bi hoan toan cd the nang cao ty sd S/N (tin hieu / dn) d mire tdt nhat Khdi xir ly tin hieu trung tam su dung bd chuyen ddi A D C cho dd phan giai 12 bit va bd chuyen ddi ciia cam bidn mien dich giai ma len tdi 1,2 m V V d i dd phan giai thiet bi cd the lay mau va hien thi chinh xac qua trinh thay ddi phan img sinh hda xay tren be mat cam bien B d M u x 8, PGA thuc hien nhiem vu chon kenh, lay m l u , khuech dai dem 06 tin hieu vao tir cong 0, ddng thdi cho phep ket 247 Nshien ciru, Phdt triin vd Uhg duns Cons nshe Vdt lieu (KC.02/06-10)- Hd Ndi 27/11/2009 qua dugc loai bo sai so moi truong va thoi gian xu ly mau tu cam bien (nguyen l y v i sai) Thiet b i giao tiep v d i may tinh thong qua cong U S B chuan H I D cho phep g u i d u lieu tir dien cue l a m viec va nhiet len may dnh K h d i x u ly cho phep giao tiep cua thidt b i v d i man h i n h L C D c h u i n cua hang^ H I T A C H I thdng qua cong cua chip C Y C ; man h i n h hien t h i ddng thdi ket qua d dien cue l a m vide cua cam bidn, nhiet cua m d i trudng do; thang va dja diem mau San pham hoan thien cd hinh dang n h u hinh Hinh Thiit bi da ddng vd IV KET L U A N Bdo cao trinh bay mot sd ket qua ban dau vide phat tridn thidt bi da kenh, xach tay ung dung cho cac cam bien sinh hoc de cac hop chat gay nhiem nude Thiet b i dugc thidt ke de l a m vide cung luc v d i cam bien tren co sd dan Cac tfnh nang tien tidn cua chip PSoC C Y C va cac chip M a x 038, L M C 6482, p A 741 dugc khai thac triet de nham cai thien ty sd tin hieu / dn ciia mach Thidt b i dugc thidt ke dd cd the phat xung, dugc tich hop san, v d i tan sd cd the thay ddi (10-30 k H z ) va bidn dd (100 mv - 300 m V ) Trong phep do, xung hinh Sin se quet tren cap dien cue lam vide T i n hieu l o i dugc tinh bang sut thd tren hai dien t r d K L L Thidt b i dang dugc thir nghiem tren cac cam bidn sinh hoc tren co sd enzim ho Cholinesterase de xac dinh d u lugng thudc tru sau nude Nhung kdt qua nghien cuu se dugc cong bd nhirng bao cao sau Loi cam on Cong trinh dugc thuc hidn v d i su hd t r g ve k i n h p h i ciia de tai KC02-05/06-10 Tai lieu tham khao M Farre, D Barcelo, T r A C , Trends A n a l Chem 22, 299 (2003) K Ramanathan, M Ensor, S Daunert, Tibtech 15, 500 (1997) D A Blake, R M Jones, I Blake, C Robert, A R Pavlov, L A Darwish, H Y u , Biosens Bioelectron 16, 799 (2001) P.A Behnisch, K Hosoe, S.-i Sakai, E n v i r o n Int 27, 413 (2001) S.S Iqbal, M W M a y o , J.G Bruno, B V Bronk, C A Batt, J.P.Chambers, Biosens Bioelectron 15 , 549 (2000) B Hock, A Dankwardt, K Kramer, A M a r x , A n a l Chim Acta 1 , 393 (1995) A Mulchandani, W Chen, P Mulchandani, J Wang, K R Rogers, Biosens Bioelectron 16 ,225 (2001) 248 Nghien ciru Phdt triin vd Uhg dims Cons nshe Vdt lieu (KC.02/06-10)- Hd Noi 27/11/2009 P.D Tam, Nghien cum che tao cam bien sinh hoc huong ung dung Uong cong nghe thuc pham va y hoc, luan an tien sy chuyen nganh Cong nghe vat lieu dien tir, Truong Dai Hoc Bach Khoa Ha Noi, 2009 T V Trung, Portable Device Detected Pesticide ConcenUation by Biosensor, MSc Thesis, Hanoi University of Technology, (2007) 11 Vu Quang Khue, Nghien cuu che tao thiet bi da kenh ung dung cho cam bien m i l n dich tten ca sa d i n , Luan van thac sy khoa hoc vat lieu, Trudng dai hoc Bach Khoa Ha Noi, (2009) 249 ... su dung chip Max 038 cho dang song sine dua vao dien cue cua cam bien co dai lam viec ve bien tir 100 -r 300 m V tan so 10 -r 30 k H z v o i muc dich co the cap t i n hieu cho nhieu loai cam bien... tan sd dua vao cam bien khac nhau, bd phat tin hieu ciia thidt bi cho phep thuc hien ghep ndi v d i nhieu bd kit ciia cam bien sinh hoc dien hda va cd the sir dung phan tir chuyen ddi la enzim... 50 m V Ket qua hien thi cho thay tin hieu khdng bi anh hudng ciia nhieu ben ngoai va cd dd sut ap tren linh kien 10 % Hinh Tin hieu V^cua bo tdch song nhay pha Didu cho phep thidt b i tach sdng