1301 Transgenesis by Lentiviral Vectors Lack of Gene Silencing in Mammalian ES Cells and Preimplantation Embryos Molecular Therapy Vol 5, No 5, May 2002, Part 2 of 2 Parts Copyright © The American Soc[.]
"7" "!"! E E -M=% / 1 89 -M=/ E "55"#'' E "55" )*)9 "55" ' E '>" E 68±9; H E -H $/ -O99&/ 95±9, H $ '>" E * G =6±95 H $% 9;±98 H $% D ::±9:H$% 59±9=H$% H ' "55"#'' "55" (*)+()9 3 E "55" ' ? ' 3 1299 Control of Immune Responses to Gene Therapy: A Human CD80 Enhancer/Promoter Construct Enables Specific Ablation of Gene-T ransfected Antigen Gene-Transfected Presenting Cells In Vitro Yuanfang Liu,1 John R Rodgers.1 Immunology, Baylor College of Medicine, Houston, Texas, United States @ G 3 " -'!/ ' 3 '! '!" 3 3 ' -$ '/ '! " ->/ " -!/ $:9 -2;8/ 3 " $:9!>" ? ! ->?!/ $ ' 3 $:9" -',9/ -@E >"$ ' ->?,/ 3 " ,5= >?, ,5= 3 3 ,5=">?, ' "$:9%$ ' 4 " ' " $:9!>%$ ' 3 3 $:9 ,5=%>?, $ '" 3 " ' "$:9!> "7" "!"! 1300 Lentiviral V ectors Utilizing the Chicken β-Globin Vectors 5′HS4 Insulator and the Human Interferonα Scaffold Interferon-α Attachment Region for High Level and Sustained Factor VIII Gene Expression Ali Ramezani,1 Teresa S Hawley,1 Robert G Hawley.1 Hematopoiesis, American Red Cross Holland Laboratory, Rockville, Maryland, United States C ' .-./ 8"&H . " 3 . 1 ' ' -C(/ . @ - / C( C E " " " $*' 3 " 3 β" &VC(6 "α -?*"('@/ G -@ G # ,76:&"65B ,999/ ->?8α/ -4= #(. D @/ -'/ 3 ->?!/ 3 $=6J A8( " " G >?! 3 ) &VC(6 ?*"('@ 3 #(. 4= D @ 1 1 $=6J % ( A8 3 >?! 2 &VC(6 ?*"('@ 3 " . $*' C( ." D" . 3 1301 T ransgenesis by Lentiviral V ectors: Lack of Transgenesis Vectors: Gene Silencing in Mammalian ES Cells and Preimplantation Embryos Alexander Pfeifer, Masahito Ikawa, Yelena Dayn, Inder M Verma @ ->(/ C 3 ? " -?'(/ 3 86 >( ?! 3 ' -D." ?!/ ?! 3 5&&H > D."?! >( 3 ?! 3 3 " Molecular Therapy Vol 5, No 5, May 2002, Part of Parts Copyright © The American Society of Gene Therapy S425 "7" "!"! D."?!" >( 3 > 3 G >( ' >( 3 * 3 G - / 3 " " ' - 8,/ 3 ? - ?!/ 3 >( ' >( " 1302 Development of B Lineage Specific Lentiviral Vectors for Use in Gene Therapy of Human B Lymphocyte Disorders Maria I Garcia-Lloret,1 Keun Chae,1 Ruby Tabuchi,1 Carolyn Lutzko,2 Don Khon,2 David J Rawlings.1 Department of Pediatrics, UCLA, Los Angeles, California, United States; 2Children′s Hospital, Los Angeles, California, United States " -(*/ 3 2 -C(/ 2 ' (* ?! ' 7 ?! 3 I 3 2 " -C2"D / C(I 3 C( 2 #. !A 3 -TB / 3 ->/ ?! 3 ="& $;5 - I 2,5/ 3 2 3 ' >2,5 ?! 3 2 3 # - #)/ 1 &"89 ?! 2 *2,5 3 2 - 2O 2O 2M 2/ C2"D >2,5 - >!A >#./ ?! 3 -,9"&9H 89",&H/ 3 -#? :99"8=99 =99"B99/ C" 3 2 #J 2 8, C2"D ($% *)$ C( * 3 ?! 3 $ 85J -2 / >2,5 ?! 1 $85J $==J- / >!A >#. >2,5 3 2 S426 1303 Optimization of HIV -1 Based Lentiviral V ectors HIV-1 Vectors for Gene-Modification of Leukemia Cells Aaron C Logan,1, Donald B Kohn.1, Division of Research Immunology/Bone Marrow Transplantation, Childrens Hospital Los Angeles, Los Angeles, CA, United States; Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, CA, United States D " # 3 " E ) 3 C."8 ##D. G " 3 -!! / C."8 " !! " 3 !! #) !! J !! " ?! G 8, 3 !! J " 3 8,",6 !! " 2 !! " 3 1 3 #. 4= #!(. 3 6"8, 2 =",& ,"= ( !! &V 3 -6"8:H / ' (.69 D @ 3 -≤&9H / ' 3 0!@> 3 2 !! (.69 3 1304 T ectors Transcriptional Targeting Vectors ranscriptional T argeting of Lentiviral V TR Enhancer Replacement LTR by L Francesco Lotti,1 Emilio Menguzzato,1 Luigi Naldini,2 Fulvio Mavilio,1 Giuliana Ferrari.1 San Raffaele-Telethon Institute for Gene Therapy (HSR-TIGET), Istituto Scientifico H.S.Raffaele, Milano, Italy; 2Institute for Cancer Research and Treatment, University of Torino Medical School, Torino, Italy 1 " -C(/ 3 C." " " D @ " Molecular Therapy Vol 5, No 5, May 2002, Part of Parts Copyright © The American Society of Gene Therapy ... 1299 Control of Immune Responses to Gene Therapy: A Human CD80 Enhancer/Promoter Construct Enables Specific Ablation of Gene- T ransfected Antigen Gene- Transfected Presenting Cells In Vitro Yuanfang... Presenting Cells In Vitro Yuanfang Liu,1 John R Rodgers.1 Immunology, Baylor College of Medicine, Houston, Texas, United States @ G