917 Helper Dependent Adenoviruses Expressing Apolipoprotein A I Do Not Alter Endothelial Cell Function In Vitro Potential for Vascular Gene Therapy Molecular Therapy Volume 17, Supplement 1, May 2009[.]
CARDIOVASCULAR randomly divided into five groups: Sham, MI+Saline, MI+MSC, MI+MSC+SAA, MI+SAA (n=20) For MSC treatment animals, immediately after LAD ligation, 2.5 millions of BrdU labeled MSCs in 150 ml of saline was injected into the border zone of the infarcted heart The control animals were only injected with 150 ml of saline After closing the chests, animals in SAA treatment groups were daily administrated with SAA (i.p., 10 mg/kg, in ml of saline for each rat) until the animals were killed for examination At days 3, animals were killed and the hearts were harvested for RNA and protein isolation The expression of MI related cytokines including HSP70, CTnT, IL-1, IL-6, TNF-a, Connexin 43 and VEGF were analyzed with Real Time PCR The protein level of HSP70, CTnT, IL-1, IL-6, TNF-a and Connexin 43 were analyzed by Western Blot At day 28, animals were examined for heart function by 2D echocardiography Heart tissues were analyzed for pathologic change, infarction size, MSCs survival and differentiation Results: Data of Real Time-PCR shows that at day the expression of IL-1, IL-6, CTnT, HSP70 and TNF-a were elevated in saline group However, the expression of these genes was inhibited to about 50-60% in MI+MSC group and MI+SAA group More interestingly, MI+MSC+SAA group showed the lowest gene expression which is only 40-50% of that in MI+MSC group and MI+SAA group, and less than 20% of that in saline group At day 30, the combination treatment group showed the highest VEGF expression and normalized CX43 expression Western blot data showed that for HSP70, Connexin 43, CTnT, IL-1, IL-6, and TNF-a expression, the difference between saline group and the three treatment groups are significant (p