Cloning strategies

Cloning Strategies Directional cloning using vector pUC119 Directly cloning of PCR products Directional cloning using vector pUC119 Cloning Sau3A fragments into a BamHI site Ligation of Sau3A-cut DNA into the λReplacement Vector EMBL4 Use of Linkers Cloning cDNA in λVector Using Linkers Use of Adaptors Homopolymer Tailing [...]...Advanced Cloning Strategies( 1) Advanced Cloning Strategies( 2) Ish-Horowicz and Burke’s cosmid cloning strategy Cloning in a YAC Vector Phage DNA and Packaging Charon Recombinant Packaging and Selection