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Nội dung
Cloning Strategies
Directional
cloning
using vector
pUC119
Directly cloning of PCR products
Directional
cloning
using vector
pUC119
Cloning Sau3A fragments into a
BamHI site
Ligation of Sau3A-cut DNA into
the λReplacement Vector
EMBL4
Use of Linkers
Cloning cDNA in λVector Using
Linkers
Use of Adaptors
Homopolymer Tailing
[...]...Advanced Cloning Strategies( 1) Advanced Cloning Strategies( 2) Ish-Horowicz and Burke’s cosmid cloning strategy Cloning in a YAC Vector Phage DNA and Packaging Charon Recombinant Packaging and Selection