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Solid state fermentation (SSF) derived cellulase for saccharification of the green seaweed Ulva for bioethanol production Solid state fermentation (SSF) derived cellulase for saccharification of the green seaweed Ulva for bioethanol production Nitin Trivedi, C R K Reddy, Ricardo Radulovich, Bhavanath Jha 1 Introduction Cellulose, a structural component of plant biomass, is the most abundant feedstock used for the production of alternative liquid fuels, mainly bioethanol Cellulose In which Cellul.
Solid state fermentation (SSF)derived cellulase for saccharification of the green seaweed Ulva for bioethanol production Nitin Trivedi, C.R.K Reddy, Ricardo Radulovich, Bhavanath Jha 1 Introduction Cellulose Cellulose, a structural component of plant biomass, is the most abundant feedstock used for the production of alternative liquid fuels, mainly bioethanol In which Cellulose from algae has high Carbohydrate content, not mixed with impurities such as Lignin, Hemi-Cellulose and Pectin, so it is easy to purify Cellulose Introduction Hydrolysis CHEMICAL HYDROLYSIS ENZYMATIC HYDROLYSIS Acid hydrolysis results in the production of some non-sugar byproducts Enzymatic hydrolysis indeed presents a green approach Introduction Objectives In this study, we isolated microbial strains that Cellulaseproduce through Solid State Fermentation (SSF) and using to product Bioethanol product Bioethanol Methods 2.1 Microorganism Cellulase positive CMC (1,5%) then Lugol’s Iodine isolate d Degraded Ulva C sphaerospermu m The molecular identification of the fungal strain was carried out by 18S rDNA sequancing Methods 2.2 Collection of algal sample Washed and dried Then grind U fasciata Powdered seaweed Methods 2.3 SSF and optimization of parameters for Cellulase production Cellulase production was optimized with: - Moisture - Incubation period - pH - temperature 250 mL Erlenmeyer flasks containing 10g of powdered seaweed Mineral salt pH Fugal spore suspension Flasks were incubated at room temperature for days Methods 2.4 Enzyme extraction and assay Suspend ed By Sodium Acetate buffer Fermented substrate Filtered Enzyme activity assay: - FPase - CMCase Cold Centrifuged The clear supernatant Enzyme stability assay: - pH - temperature Methods 2.5 Hydrolysis of algal biomass through SSF-derived cellulase SSF-derived Cellulase Cellulose from U fasciata Reducing sugar Spectrophotometric lly using DNS method ORANGE Methods 2.5 Hydrolysis of algal biomass through SSF-derived cellulase SSF-derived Cellulase Reducing sugar Cellulose from U fasciata Enzyme dosage Hydrolysis temperature OPTIMIZATION OF pH Incubation period Spectrophotometric lly using DNS method Methods 2.5 Hydrolysis of algal biomass through SSF-derived cellulase SSF-derived Cellulase Reducing sugar Cellulose from U fasciata Qualitatively analyzed using TLC Spectrophotometric lly using DNS method Methods 2.6 Fermentation of algal hydrolysate Saccharomyces cerevositae MTCC No 180 Reducing sugar (from algal hydrolysate) Bioethanol Using GC-MS and DNS method to analyzed Ethanol yield and residual reducing sugars Results 3.1 Optimization of SSF for enzyme production Inoculated fungus with 60% moisture content, incubated for days at 25 °C and pH 4, showed optimum enzyme production 3 Results 3.2 Effect of pH and temperature on cellulase activity and stability Enzyme activity, found to be optimal at pH and 40 °C Results 3.3 Hydrolysis of algal biomass through SSF-derived cellulase Optimization of algal biomass with: - 10 U/g Enzyme dosage - 24h period - pH - 40ºC incubation Enzyme dosage (U/g) Results 3.3 Hydrolysis of algal biomass through SSF-derived cellulase The presence of Glucose in the hydrolysate, determined with TLC Standard Glucose Algal Cellulose Algal Biomass Results 3.4 Fermentation of algal hydrolysate Conclusions The optimal conditions SSF is a dry algal substrate containing inoculated fungus with 60% moisture content, incubated for days at 25 °C and pH The optimal conditions for fermenting aldal biomass are 10 U/g enzymes, incubation period of 24 h, hydrolysis temperature of 40 °C and pH The optimum ethanol yield was found to be 0.44g with 93.81% conversion efficiency after 12 h of fermentation 15 % Thanks ! Do you have any questions? CREDITS: This presentation template was created by Slidesgo, including icons by Flaticon and infographics & images by Freepik ... through Solid State Fermentation (SSF) and using to product Bioethanol product Bioethanol Methods 2.1 Microorganism Cellulase positive CMC (1,5%) then Lugol’s Iodine isolate d Degraded Ulva C... optimization of parameters for Cellulase production Cellulase production was optimized with: - Moisture - Incubation period - pH - temperature 250 mL Erlenmeyer flasks containing 10g of powdered seaweed. .. Hydrolysis of algal biomass through SSF -derived cellulase The presence of Glucose in the hydrolysate, determined with TLC Standard Glucose Algal Cellulose Algal Biomass Results 3.4 Fermentation of algal