Pectinase production by Aspergillus niger using banana (Musa balbisiana) peel as substrate and its effect on clarification of banana juice Giảng viên: Nguyễn Đức Chung Bộ môn: Công nghệ enzyme Lớp: CNTP 52C Khoa: Cơ khí cơng nghệ I INTRODUCTION Pectinase is a general term for enzymes, such as pectin lyase, pectin methylesterase and polygalacturonase, commonly referred to as pectic enzymes These break down pectin, a polysaccharide substrate that is found in the cell walls of plants The pectinase enzyme is obtained mainly by fungi, in which Aspergillus niger is believed to be capable of producing many pectinase enzymes when cultured under suitable media I INTRODUCTION Polygalactorunase obtained from Aspergillus niger cultured on banana peel substrate has the effect of making banana juice To optimize crude pectinase production using Bhimkol banana peel as substrate for higher polygalacturonase activity II Materials Banana peel is an abundant and low cost agricultural waste residue It accounts for about 40 % of the weight of the raw fruit and is rich in carbohydrates, protein and various vitamins and mineral elements They have been used for the production of pectinases number ingredient Content (%) protein 0,9 Crude lipid 1,7 Carbohydrate 59 Crude fiber 31,7 Cellulose 8,5 Pectin 10-24 III Pectinase production: Substrate preparation for enzyme production: - The bananas were washed under tab water and peeled with hand The peels were then dried at 45 °C for 24 h and then grinded using commercial grinder and passed through 200 mesh sieve to obtain banana peel powder The powder thus produced was then used as substrate for the production of Pectinase enzyme III Pectinase production: Pectinase production: Experiments were carried out in 250 ml Erlenmeyer flasks containing KH2PO4 (0.02 % w/v), MgSO4 (0.01 % w/v), (NH4)2 SO4 (0.04 % w/v), FeSO4 (0.01 % w/v), MnSO4 (0.001 % w/v) in 100 ml distilled water The medium was sterilized and cooled to room temperature The pH of the medium: 5,8 (using citrate buffer) The sterilized flasks were inoculated incubation in an incubator shaker 2 × 108 spores of A niger speed of 150 rpm incubation the culture filtrate was cooled to 4 °C in refrigerated centrifuge centrifuge pectinase 10,000 rpm for 10 min III Pectinase production: Partial crude enzyme: - GIỚI THIỆU NGUYÊN LIỆU PHƯƠNG PHÁP 1 CHUẨN BỊ CƠ CHẤT ĐỂ SẢN XUẤT ENZYME 2 ... INTRODUCTION Polygalactorunase obtained from Aspergillus niger cultured on banana peel substrate has the effect of making banana juice To optimize crude pectinase production using? ?Bhimkol? ?banana peel. .. 10-24 III Pectinase production: Substrate preparation for enzyme production: - The bananas were washed under tab water and peeled with hand The peels were then dried at 45 °C for 24 h and then... using commercial grinder and passed through 200 mesh sieve to obtain banana peel powder The powder thus produced was then used as substrate for the production of Pectinase enzyme III Pectinase