Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"

... proteins induced by hypoxia in trophoblastic cells so as to clarify the mechanism of hypoxia-induced apoptosis by using the PoweBlot, an antibody-based Western array. Human choriocarcinoma cell line ... ap-proach to identify key molecules in the course of hy-poxia-induced apoptotic pathways. Such analysis of trophoblastic cells will, in the near future, yield in- sights...

Ngày tải lên: 31/10/2012, 15:12

... confirmed by sequencing DNA samples of 51 healthy individuals. Interestingly, this SNP had not yet been registered in any SNP databases despite of its high frequency of 20% in samples of patients ... Department of Biochemistry of the Charité, Berlin, Germany using the ABI PRISM Big Dye Terminator system (Applied Biosystems, Germany). Sequences were analyzed using the Chromas s...

Ngày tải lên: 31/10/2012, 16:49

... immunoglobulin chains were also found to be significantly increased in aged thymocytes. Understanding the changes in gene expression in thymocytes with age may hold the key in determining thymocyte fate ... expression in thymocytes with age. As shown in Table 4, real time RT-PCR confirmed the increased levels of many of these genes identified in the array analysis. Moreover...

Ngày tải lên: 03/11/2012, 11:35

... Methylpyridinium (MPP(+))- and nerve growth factor-induced changes in pro- and anti-apoptotic signaling pathways in SH-SY 5Y neuroblastoma cells. Brain Res 952, 98–110. 42 Yuan Y, Jin J, Yang ... that possibly grouped together all the proteins differentially expressed. Instead of validating every single protein by western blotting, we applied a different approach by searching for know...

Ngày tải lên: 15/02/2014, 01:20

... residues phenylalanine and tyrosine typical of the Ig-fold are bold in each domain. Fig. 5. Inhibition of in vivo myotoxicity of myotoxins I or II by DM64. Groups of four mice were injected intramuscularly ... first three domains and are present in the inhibitory receptors of Fig. 6. Inhibition of in vitro cytotoxicity of myotoxins I or II by DM64. Cytotoxicity was analyz...

Ngày tải lên: 21/02/2014, 01:21

... Mchaourab, H.S. (1999) Folding pattern of the a-crystallin domain in aA-crystallin determined by site-directed spin labelling. J. Mol. B iol. 294, 561–577. 68. To ¨ ro ¨ k, Z., Goloubinoff, P., Horva ´ th, ... [66], and disassembly of active units from an oligomeric (storage) state of a-crystallin was proposed, upon structural analysis of aA-crystallin by site- directed spin labell...

Ngày tải lên: 22/02/2014, 04:20

... belongs to a family of enzymes that catalyze protein cross-linking reac- tions and is constitutively expressed in many tissues as well as being activated during apoptosis. In a recent paper, ... indicating either a direct interaction with the triad amino acids or a consequence of steric hindrance causing an interference in enzymatic activity. In conclusion, by analyzing individua...

Ngày tải lên: 08/03/2014, 09:20

... 2-N-(3-O- acyl 4 -acyl 2 )-b-GlcN-(1–6)-2-N-acyl 1 )3-O-acyl 3 -GlcN where acyl 1 ,acyl 2 and acyl 3 are 3-hydroxyhexadecanoic or 3-hy- droxyoctadecanoic acids, acyl 4 is tetradecanoic or (minor) hexadecanoic ... C-1, and was mostly present in an a-pyranose form. Acyl 1 ,acyl 2 and acyl 3 residues had hydroxy or acyloxy groups at C-3 ( 13 C signals of C-3 at 69.0–72.4 p.p.m.), while acyl...

Ngày tải lên: 08/03/2014, 09:20

... core-oligosaccha- rides of E. coli F653 LPS for which only preliminary data were available and investigated the deacylated LPS by NMR and MS. The proposed structure determined pre- viously by methylation analysis ... Verhoef, J. & Sinnwell, V. (1988) Iolation and chemical analysis of 7-O-(2-amino-2-deoxy-a- D -glucopyranosyl- L -glycero- D -manno-heptose as a constituent of the...

Ngày tải lên: 08/03/2014, 09:20

... Kinetic analysis of hydroxylation of saturated fatty acids by recombinant P450foxy produced by an Escherichia coli expression system 7 Tatsuya 7 Kitazume 1 , Akinori Tanaka 1 , Naoki Takaya 1 , ... substrate-accessing channel, and their guanidinium and hydroxyl groups, respectively, play crucial roles in the binding of the substrate by interacting with the carboxyl group of the su...

Ngày tải lên: 08/03/2014, 22:20