Capillary electrochromatography

. monograph [26] has recently also been published. 2 Concept of Capillary Electrochromatography Capillary electrochromatography is a high-performance liquid phase. strumentation, capillary column technology,separation conditions, and examples of a variety of applications are discussed in detail. Keywords. Capillary electrochromatography,

Ngày tải lên: 21/08/2013, 10:23

... modelling In this study, the problem of a liquid (e.g. water) rising in a cylindrical capillary tube by the effect of capillary force is considered. Initially, the tube is filled with porous media (e.g. ... (1) where h is the CR height, K is the permeability, L is the length of the capillary tube, c P is the macroscopic capillary pressure, φ is the porosity, 1 µ and 2 µ are the viscos...

Ngày tải lên: 05/09/2013, 17:03

... °C until use. Peptide mapping by capillary liquid chromatography microelectrospray tandem MS (LC-lESI-MS/MS) Tryptic peptides were analysed using a self-packed capillary column (0.1 · 120 mm, ... & Shore G. (2000) Mol. Cell. Biol. 20, 6731–6740). We describe here the char- acterization by capillary liquid chromatography microelec- trospray tandem MS of a BAP31 immunocomplex isolated...

Ngày tải lên: 20/02/2014, 23:20

... correct 525.2-1 METHOD 525.2 DETERMINATION OF ORGANIC COMPOUNDS IN DRINKING WATER BY LIQUID-SOLID EXTRACTION AND CAPILLARY COLUMN GAS CHROMATOGRAPHY/MASS SPECTROMETRY Revision 2.0 J.W. Eichelberger, T.D. Behymer, ... Compounds 525.2-2 METHOD 525.2 DETERMINATION OF ORGANIC COMPOUNDS IN DRINKING WATER BY LIQUID-SOLID EXTRACTION AND CAPILLARY COLUMN GAS CHROMATOGRAPHY/MASS SPECTROMETRY 1.0 S...

Ngày tải lên: 22/03/2014, 14:20

... were analyzed by capillary zonal electrophoresis. Samples (2 nL) were injected under vacuum into a PACE 5000 capillary electrophoresis system (Beckman) equipped with a silica capillary (length ... absence or presence of selective inhibitor using capillary zonal electrophoresis, several parameters have to be established. Fig. 1 shows the capillary zonal electrophoresis profile of FMRF...

Ngày tải lên: 23/03/2014, 12:20

... uncoated fused silica capillary with 50 cm to the detector window and of 57 cm total length. Separations were carried out at 17 or 18 kV corresponding to a current of approximately 85 lA at a capillary temperature ... features associated with protein or peptide heparin binding. Here, we characterize the bind- ing using capillary electrophoresis (CE), surface plasmon resonance (SPR), and...

Ngày tải lên: 31/03/2014, 23:20

... detector end of the capillary. A negative voltage is then apphed, causmg the sample components to mtgrate back along the capillary, pass through the detector, and emerge from the capillary at the ... at the sample mtroduction end of the capillary. Again the voltage IS stopped immediately prior to emergence of the sample zone from the capillary, and the capillary is dipped into a...

Ngày tải lên: 10/04/2014, 11:11

... conditions, capillary electrophore- sis has the resolving power to readily separate O-GlcNAc, O-phosphate, and unmodified peptides from each other (32). We anticipate that the combined use of capillary ... review of capillary electrophoresis of carbohydrates. Electrophoresis 19, 2539–2560. 51. Soga, T. and Heiger, D. N. (1998) Simultaneous determination of monosaccha- rides in glycoprot...

Ngày tải lên: 10/04/2014, 11:11

... could be quantitatively estimated (96). 14 Mitchelson 5.2. Capillary Affinity Electrophoresis 5.2.1. Capillary Affinity Gel Electrophoresis Capillary affinity gel electrophoresis (CAGE) is an electrophoretic ... Electrophoresis 19, 3154–3159. 19. Chiari, M. and Cretich, M. (2001) Capillary coatings: choices for capillary electrophore- sis of DNA, in Capillary Electrophoresis of Nu...

Ngày tải lên: 10/04/2014, 11:11

... Mitchelson Jing Cheng Capillary Electrophoresis of Nucleic Acids VOLUME 163 Volume II Practical Applications of Capillary Electrophoresis Edited by Keith R. Mitchelson Jing Cheng Capillary Electrophoresis of ... solutions. 2. Materials 2.1. Capillary Electrophoresis 1. Fast capillary, fused silica (effective length): 3 cm × 50 µm (id), (DB-1, J&W Scien- tific, CA). 2. Capillary...

Ngày tải lên: 10/04/2014, 11:11