DNA Replication in Prokaryotes

... and 1L8Q (DnaA) The AAA+ domains of both proteins are shown in cyan with ADP indicated in red The helix-turn-helix (HTH)-containing DNA binding domain of DnaA is in green and the winged helix ... C-terminal DNA binding domains (DBDs) In DnaA, the DBD contains a helix-turn-helix; in the archaeal proteins, the DBD has a winged helix domain (reviewed in [62])...

Ngày tải lên: 07/03/2014, 21:20

... support DNA replication of a plasmid carrying the JCV origin Columns and 3, SV40 Tag with human and murine DNA polymerase a-primase, respectively; columns and 5, JCV Tag with human and murine DNA polymerase ... slightly inhibited by the murine enzyme complex Fig Autoradiogram of an in vitro JCV DNA replication initiation assay with 0.2 and 0.4 units...

Ngày tải lên: 17/03/2014, 03:20

... alternative mechanisms for initiation of DNA replication forks in bacteriophage T4: Priming by RNA polymerase and by recombination Proceedings of the National Academy of Sciences of the United States ... bacteriophage T4 DNA replication and replication fork dynamics: a review in the Virology Journal series on bacteriophage T4 and...

Ngày tải lên: 11/08/2014, 21:21

... Cite this article as: Mueser et al.: Structural analysis of bacteriophage T4 DNA replication: a review in the Virology Journal series on bacteriophage T4 and its relatives Virology Journal 2010 ... duplex DNA likely explains the sensitivity of T4 ligase activity to salt concentration Conclusion and Future Directions of Structural Analys...

Ngày tải lên: 11/08/2014, 21:21

... viral DNA, reduced in cellular viral RNA (cRNA), and lowest in virion RNA (vRNA), indicating a gradient of hypermutation We surmise that purifying selection at multiple steps during viral replication ... indicated that purifying selection pressure was operating against genomes that had inactivating mutations in the gag gene The observation...

Ngày tải lên: 13/08/2014, 05:21

... genomes analyzed in this study, suggesting the existence of a previously unrecognized regulatory network coupling DNA replication and translation in Archaea Many putative DNA replication proteins have ... oligonucleotide/oligosaccharide-binding (OB)-fold containing protein, a recently described new GTPase, DnaG) are encoded by genes that co-localize with DNA replication...

Ngày tải lên: 14/08/2014, 08:21

... Phase and G2/M Checkpoints: In the budding yeast, DNA damage surveillance mechanisms operate in G1, S and G2 phases These are the G1 DNA damage checkpoint, the S phase replication and DNA damage checkpoints, ... REGULATION OF SPINDLE BEHAVIOR BY DNA REPLICATION AND DAMAGE CHECKPOINTS IN BUDDING YEAST SAURABH RAJENDRA NIRANTAR (B.Tech.(Hons)...

Ngày tải lên: 14/09/2015, 14:03

... GBD-hCdt1 binds strongly with RID-hGeminin - 67 3.7 Crystallization of Cdt1 /Geminin complex .- 68 Chapter4 Discussions - 71 4.1 Identifying the Geminin Binding Domain of hCdt1 ... ORC CDT1 Pre-RC S phase (Activation) CDKs Geminin (Licensing) Figure2.1 DNA replication licensing control by Geminin and CDKs during the cell cycle In metazoa, Geminin, present from the onset ......

Ngày tải lên: 04/10/2015, 10:24

... tools to study the force and motions of biological molecules such as DNA and proteins, and DNA- protein interaction in nanometer scale In particular, the interaction between DNA and proteins has ... histone protein to prevent the misfolding of histone protein and consequently avoid aggregating into nonfunctional structures during chromosome compaction Histone ch...

Ngày tải lên: 12/10/2015, 17:34

... deciphering the origin of the destabilization and hence, diminution of the melting temperature (Tm) in the former Base stacking in the vicinity of A C mismatch in PNAÆDNA and DNA duplexes Intra strand ... stacking, fluctuating nature of the hydrogen bond and water organization in the vicinity of the mismatch might be the contributing factors for the...

Ngày tải lên: 07/03/2014, 21:20

... late-replicating heterochromatin, where it translates the DNA methylation mark into a histone modification (deacetylation) pattern Another connection between chromatin assembly and DNA methylation ... transient euchromatic modification pattern and establish silencing after replication Propagation of chromatin states during replication In larger eukaryotes, the restoration of chroma...

Ngày tải lên: 16/03/2014, 16:20

... in the binding of DAPI [16] In conclusion, the main cause of the strong inhibitory effect of DAPI on DNA replication in vitro reported in this work appears to be linked to the propensity of the ... sequences within introns can regulate transcription [19,20], the prevalent location of ATT triplet repeats within introns in the human genome [18] su...

Ngày tải lên: 16/03/2014, 23:20

... topoisomerase I is activated or DNA gyrase is inhibited to compromise DNA, and equal to: Hsupercoiling v ˆ kt v kt topoisomerase topoisomerase gyrase gyrase esupercoilingI ‡ esupercoiling I À esupercoiling ... esupercoiling À v kt v kt topoisomerase topoisomerase gyrase gyrase esupercoilingI ‡ esupercoiling I À esupercoiling À esupercoiling …4† The elasticities of...

Ngày tải lên: 18/03/2014, 01:20

... tissue-specific methylation events in mice [16,17] and humans [9,18–20] Thus, the extent of DNA methylation appears to change in a systematic way during mammalian development DNA methylation status may be in uenced ... mechanisms involved in tissue differentiation are unknown Intragenic DNA methylation is known to be capable of altering the chromatin structure and elonga...

Ngày tải lên: 23/03/2014, 07:20