Ebook Organic structure determination using 2D NMR spectroscopy Part 2

... the length of loop I is longer in short neurotoxins The NMR structure of NTX-1 is closest to that of the a-cobratoxin crystal The superposition of these two ˚ structures shows an RMSD value of ... similar They all show a conformational variability at the tip portion of loop II, with the difference being that the length of the b-strands in NTX-1 is longer th...

Ngày tải lên: 23/03/2014, 13:20

... by Enhanced Amide Hydrogen -Deuterium Exchange Mass Spectrometry (DXMS) Blood 100:259a xviii xix ABSTRACT OF THE DISSERTATION FACILITATION OF PROTEIN 3-D STRUCTURE DETERMINATION USING ENHANCED PEPTIDE ... labeling of proteins via amide hydrogen /deuterium exchange followed by proteolytic fragmentation of labeled protein and analysis by mass spect...

Ngày tải lên: 13/11/2014, 16:12

... been developed with the goal of obtaining structural and Macromolecular NMR for the non-spectroscopist II dynamic information from such systems [79–83] As for solution- state NMR, the development ... even though no information can be obtained about the site on the protein receptor to which the ligand binds In STD -NMR, the interaction is monitored by observing the N...

Ngày tải lên: 29/03/2014, 00:20

... DEVELOPING SOFTWARE TOOLS FOR STRUCTURE DETERMINATION OF LARGE PROTEINS BY NMR SPECTROSCOPY ZHANG LEI B.SC (HONS.), NUS A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE GRADUATE ... cycle of refinement may go on several times before a high resolution structure can be determined Figure 1.5: Outline of the procedure for protein structure determinat...

Ngày tải lên: 04/10/2015, 15:45

... produced by cone voltage fragmentation [14] The fragment ions of m/z 729 and m/z 544 are both from the monofucosylated D2b-5 ion m/z 1037 and not from the difucosylated D2b-5 ion m/z 1183 Thus the ... iLNO core structures and its variously fucosylated analogues, and to determine their anomeric configurations and linkages The proton chemical shifts of Ó FEBS 20...

Ngày tải lên: 19/02/2014, 12:20

... design The TRAIL agonist (TNF related apoptosis inducing ligand receptors) Inhibitors of Bcl-2 family proteins [57, 58] Inhibitors of IAP proteins (inhibitors of apoptosis) Inhibitors of MDM2 ... at the protein to ligand ratio of 1:1, whereas saturation could not be reached for G94 3.3.4.3 Binding site analysis using NMR based parameters The mapping of bin...

Ngày tải lên: 14/09/2015, 08:49

... properties of the hNck2 SH3 domains 1. 5.2 Secondary structure transition study of hNck2 SH3- 1 domain The unfolding of SH3- 1 induced by the pH change and transition from the β-sheet dominant to the helical ... main binding region of DOCK180 was located at region 18 19 -18 36 In addition, two of the other weak binding sites were pinpointed at the...

Ngày tải lên: 14/09/2015, 14:09

... relaxation delays of 10, 30, 45, 60, 75, 90 and 150ms 15 N T1 and T2 relaxation times and {1H}-15N steady-state NOEs of SH3- 1-V 22 52 (insertion of Val at 22 nd) were determined at 20 °C 15 N T1 values ... delays of 10, 500, 50, 300, 100, 400 and 20 0ms 15 N T2 values were determined with relaxation delays of 10, 50, 80, 120 , 150, 180, 21 0, 23 0 and 25 0 ms 15...

Ngày tải lên: 14/09/2015, 14:09

... characteristic of all SH3 domains Figure 3. 3C shows a superimposition of hNck2 SH3- 2 and Nck1 SH3- 2 domains recently 60 Figure 3. 2 HSQC and sequential assignments of SH3- 2 and SH3- 3 A shows the HSQC and ... 0 .37 Secondary structure regions 64 3. 2.2 HSQC identification of the residues involved in binding of the hNck2 SH3 Domains The inte...

Ngày tải lên: 14/09/2015, 14:09

... β-sheets The first, the last and the first half of the second β-strand constitute the first β-sheet, whereas the second half of the second β-strand, together with the third and fourth β-strands constitutes ... with the lowest target function and its ensemble of ten structures superimposed over residues 32 44 Right: Ribbon representation of the NMR structur...

Ngày tải lên: 14/09/2015, 14:10

... HG Val53 HN Tyr52 HN Glu54 HN Arg 55 HN Glu54 HN Val53 HN Glu54 HN Tyr52 HB Arg 55 HN Val53 HG Arg 55 HN Val53 HB Table 5. 2 Native like long range NOEs Between the first and second beta strands Val3 ... between the SH3- 1-V22 and WT at and 1H frequencies, especially at the N- and C-terminals of SH3- 1 1 25 Figure 5. 3 Assignments of SH3- 1-V22 at pH 4.0 and V22△...

Ngày tải lên: 14/09/2015, 14:10

... both the CD and NMR spectra; the NMR titration experiment shows that the binding event mainly happens in the Cterminal of the ApLLP; 2) Consistent with the NMR study, the CD spectra show that there ... 159 Figure 6. 5 Bindings of the CRE DNA to two ApLLP proteins A–B) Superimposition of the 1H–15N NMR HSQC spectra of ApLLP-87, A) and ApLLP-55, B) in...

Ngày tải lên: 14/09/2015, 14:10

... 7. 2 Future work 1) Structure determinations and study of binding diversity are carried out for both SH3- 2 and SH3- 3 domain in this project However, whether the hydrogen bond ... between SH3 and proline-rich peptides The dynamic information of loops and 310-helix in the complex system will give us more functional implication 3) If the solubility and stabilit...

Ngày tải lên: 14/09/2015, 14:10

... N-Acetyl aspartate (NAA), N-Acetylaspartylglutamate (NAAG), Alanine, Choline, Creatine, Glutamate, Glycine, Myo-inositol, Lactate, Pyruvate, Serine, Threonine, Tyrosine and Valine Acetate is an ... Magnetic resonance spectroscopy correlation with histological analysis in gliomas and structure determination of a hypothetical protein Xu Ying A THESIS SUBMITTE...

Ngày tải lên: 10/11/2015, 11:35