solidstate nmr spectroscopy on complex biomolecules
Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc
... a,b-anor-meric configuration. However, because of the complexityof overlapping signals of the same monosaccharideresidues in a very similar chemical environment, it isdifficult for NMR alone to assign ... [17], one trifucosylated [23], one tetrafucosyl-ated [24], and one pentafucosylated [24] oligosaccharides.Acidic structures described include one monosialyl-mono-fucosylated [25], two monosialyl-difucosylated ... gel-filtration and ion-exchange columns with distilledwater. The partially resolved octasaccharide to undeca-saccharide fractions were further fractionated by normal-phase HPLC on a preparative Separon...
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Tài liệu Báo cáo khoa học: Allosteric modulation of myristate and Mn(III)heme binding to human serum albumin Optical and NMR spectroscopy characterization pptx
... 0.5%.1H nuclear magnetic relaxation dispersion (NMRD) pro-files were recorded at variable concentration of myristateby measuring water proton longitudinal relaxation rates(R1obs) at magnetic ... con-centration of the paramagnetic metal ion, and T1Mis thelongitudinal relaxation time of localized water protons[20,36].Relaxivity of Mn(III)heme–HSA solutions at 25.0 ° Casa function ... 46724683 ê 2005 FEBS 4681 Contributions to relaxation differ, depending on theconformational state of HSA and on the occupancy ofthe myristate binding sites. The relaxation rate changeis highest...
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Báo cáo khoa học: Epitope mapping of the O-chain polysaccharide of Legionella pneumophila serogroup 1 lipopolysaccharide by saturation-transfer-difference NMR spectroscopy pot
... experimentalconditions, d espite a r elaxation delay of 4.3 s and a satu rationtime of 3 s. Nevertheless, saturation transfer to the N-methylgroups was not observed under these conditions. A satura-tion ... legionaminic acid was 1 : 1 in long-chain and 1 : 2 inmiddle-chain PSNH4OH/HF, respectively. Based on the rela-tive intensities of the proton signals it was concluded thatonly one legionaminic ... exchange could be observedunder the conditions applied. From these data it is conclud edthat under certain conditions it is possible to observe theinterconversion of the cis and trans N-methyl...
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Báo cáo khoa học: Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy pdf
... (1991) Assignments of backbone1H,13C, and15Nresonances and secon dary structure of ribonuclease H fromEscherichia coli by heteronuclear three-d imensional NMR spec-troscopy. Biochemistry ... expression systems based on Escheri chia coli extracts[2,10,15,16]. At these yields, protein concentrations aresufficiently high t o allow the recording of NMR spectrawithout further concentration ... gene, using a combination of ligation andrecursive overlap extension of complementary syntheticoligonucleotides as described above. Oligonucleotides usedfor the construction of the s-CYPA are...
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Báo cáo Y học: Purification, crystallization, NMR spectroscopy and biochemical analyses of a-phycoerythrocyanin peptides pptx
... typicalcrystallization conditions comparing the E-andtheZ-configurations are demonstrated in Fig. 3. Despite theidentical crystallization conditions, only the E-configurationcrystallized (Fig. ... fraction (Fig. 1),only the two naturally occurring PEC-linker complexes arepresent, confirming the brief dissociation and separationconditions [4]. The second important preparation step wasthat ... Z-andE-configurations of one chromopeptide with an excellentsolubility and stability were investigated by 1D NMR spectroscopy. The information presented here are mainlyfocused on the preparations,...
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Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot
... transition underconditions of slow exchange on the chemical-shift timescale,the dissociation constant, Kd, is given byKdẳẵPẵL=ẵPLHere, [P], [L] and [PL] are the respective concentrationsof ... exchange regime. Thus, theconfiguration at the hemiacetal carbon of galactosemay affect the dissociation constants.By contrast, because chemical-shift changes uponthe addition of sugars at subdomain ... pulses for saturation with1 ms intervals and a total saturation time of 2 s. On- res-onance irradiation of the protein was conducted at a chemi-cal shift of –0.4 p.p.m. and off-resonance at a chemicalshift...
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Báo cáo khoa học: Solution structure of long neurotoxin NTX-1 from the venom of Naja naja oxiana by 2D-NMR spectroscopy pot
... NTX-1were confirmed by electron spray ionization/mass spectro-metry (ESI-MS). NMR spectroscopy NTX-1 was dissolved in D2O/H2O (10 : 90, v/v) pH 3.2 or99.9% (v/v) D2O to the final concentration ... flexibility [37]. On the other hand,the substitution o f S er in other long neurotoxins with G ly33in NTX-1 (Fig. 1) at the tip portion of loop II makes thisregion conformationally more variable.Binding ... possible explan-ation for the lower dissociation constants of long neuro-toxins from nicotinic acetylcholine receptor c ompared toshort ones.Fig. 6. Comparison of NTX-1 NMR and 1NTN X-ray...
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Báo cáo khoa học: Functional genomics by NMR spectroscopy Phenylacetate catabolism in Escherichia coli docx
... expressionof downstream genes, as appropriate. The expected con-sumption of phenylacetate was confirmed by HPLC ofthe culture fluid using online liquid-scintillation countingfor detection (data ... strains grew on succinate, but nonegrew on adipate.Transformation of labeled phenylacetate into labeledproducts by the mutantsMutant cell suspensions were incubated in buffer contain-ing ... carbon atoms (122.1, 123.1, 126.5 and129.3 p.p.m.; Table 1); they were all correlated to directlyattached protons as shown by 2D HMQC spectroscopy (Fig. 4). Carbon connectivities established on...
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Báo cáo khoa học: Macromolecular NMR spectroscopy for the non-spectroscopist pot
... deviations of bond lengths,bond angles and dihedral angles from ideality, unfa-vourable sidechain rotamers, and bad nonbondedinteractions. molprobity additionally offers all-atomcontact analysis ... ‘resolution’ of NMR- derived protein structures.Assessment criterion Very high resolution High resolution Medium resolution Low resolutionRestraints per residuea> 18 14–18 10–15 < 10Backbone ... macromolecular NMR spectroscopy, including basic interpretation of commonly encoun-tered NMR spectra. We then outline the process byKeywordsHSQC; nuclear magnetic resonance (NMR) spectroscopy; ...
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Báo cáo khoa học: Macromolecular NMR spectroscopy for the non-spectroscopist: beyond macromolecular solution structure determination potx
... on the existence of motions on the ps tons timescale, whereas R2additionally depends on slower motions on the ls to ms timescale. These datacan be analysed [41–43] to separate contributions ... VTS1p–RNA interac-tion is driven by an increase in conformational entropy.Slower motions (ls-ms)As noted above, R2relaxation rates can provide infor-mation on slower internal motions in proteins.Related, ... andsuch motion can occur on timescales ranging from pico-seconds to seconds or more.Fast motions (ps–ns)These motions are accessible through the measurementof the relaxation rate constants for...
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Báo cáo khoa học: Structural function of C-terminal amidation of endomorphin Conformational comparison ofl-selective endomorphin-2 with its C-terminal free acid, studied by 1 H-NMR spectroscopy, molecular calculation, and X-ray crystallography pot
... [3], theactive conformation of EM2 should be taken into con-sideration on the basis of not only the conformationaldifference in the backbone chain but also the overallconformation, including ... to C-terminal amidation.Solution conformation by NMR spectroscopy andsimulated annealing calculationThe conformational features of EM2 and EM2OH,obtained by the present NMR measurements andmolecular ... dockingmodels were constructed for the open conformers andsubjected to energy evaluation for reasonable configur-ation and translation exploration using the Dockingmodule of insight discover. Consequently,...
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Báo cáo Y học: Conformational analysis by CD and NMR spectroscopy of a peptide encompassing the amphipathic domain of YopD from Yersinia potx
... function is unknown, it isdependent on an interaction with the nonsecreted TTSSchaperone LcrH [12,13]. This interaction is responsible forthe presecretory stabilization and efficient secretion ... determination of a TTSS chaperone/effector protein complex from Salmonella spp., it is likelythat at least one function of chaperones is to maintain theircognate partner in an elongated unfolded ... application to proton correlation spectroscopy. J. Magn. Reson. 53, 521–528.25. Jeener, J., Meier, B.H., Bachmann, P. & Ernst, R.R. (1979)Investigation of exchange processes by two-dimensional NMR spectroscopy. ...
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