Methods in molecular biology vol 1542 mycotoxigenic fungi methods and protocols

... bridging protein consisting of the immunoglobulin G (IgG)-binding β2 domain of streptococcal protein G modified by genetic fusion with the positively charged leucine zipper interaction domain (PG-zb), ... III Series: Methods in molecular biology (Clifton, N.J.) ; 303 TP248.25.N35N35 2005 660.6 dc22 2005000473 Preface Increasingly, researchers find themselves involved in discipline-spa...

Ngày tải lên: 04/06/2014, 15:14

Ngày tải lên: 08/08/2014, 14:21

... Crowding influences Intra-cellular Trafficking 6.4 Macromolecular Crowding on Protein-folding and Stability 6.5 Macromolecular Crowding Effects on Protein-Aggregation 6.6 Macromolecular Crowding Effects ... frequently increasing the binding strength by at least an order of magnitude (Wilf & Minton, 1981) 6.4 Macromolecular Crowding on Protein-folding and Stability Crowding influences...

Ngày tải lên: 11/09/2015, 09:02

... F.1.2 Lipid Kinase Assay F.1 .3 Protein Kinase Assay F.1.4 Protein Tyrosine Phosphatase Assay F.1.5 Alkaline Phosphatase Assay F.1.6 Caspase Assay F.2 Functional Assays F.2.1 Apoptosis Assay F.2.2 ... Proliferation Assay [(2 , 3- Bis (2-methoxy-4-nitro-5-sulfophenyl)2H-tetrazolium-5-carboxanilide)] F.2 .3 Chemotaxis Assay F.2.4 Matrigel Invasion or Chemoinvasion Assay Notes 97 100 G Autora...

Ngày tải lên: 27/06/2014, 10:20

... different methods are available: solution assays such as colorimetric assays for hexose and sialic acid; membrane-based methods such as slot-blotting and staining with periodic acid-Schiff reagent; ... on-line with a UV monitor and collect fractions using a fraction collector and subject to the appropriate carbohydrate and antibody analyses 3.6 Analysis of Mucins Methods for t...

Ngày tải lên: 08/10/2012, 10:25

... to T-antigen (β-d-Gal 1-3 GalNAc), but also to structures found within the backbone of oligosaccharides (β-d-Gal 1-3 / 4GlcNAc) (19) Demonstration of PNA binding is not necessarily evidence of T-antigen ... W., Coret, E., Trueman, L., and Clay, M G (1984) Histochemical identification of side chain substituted O-acetylated sialic acids: the PATKOH-Bh-PAS and the PAPT-KOH-Bh-PAS procedu...

Ngày tải lên: 08/10/2012, 10:25

... gel-forming mucins, in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ Carraway, K L (2000) Preparation of membrane mucin, in Glycoprotein Methods and Protocols: The ... chromogens that leave a colored precipitate on the blot (e.g., 3,3'-diaminobenzidine, 4-chloronapthol mix for HRP [14]; 5-bromo-4-chloro-3'-indolyphosphate toluidine salt,...

Ngày tải lên: 08/10/2012, 10:25

... phenol in 70 mL of glycerol Poly-L-lysine-coated slides: slides are immersed in 0.01% (w/v) poly-L-lysine for and dried at room temperature Methods 3.1 Observation and Measurement of Mucous Thickness ... adherent to rat, frog and human gastric mucosa: influence of feeding prostaglandin, N-acetyl-cysteine and other agents Clin Sci 63, 187–195 Bell, A E., Allen, A., Morris, E R., and...

Ngày tải lên: 08/10/2012, 10:25