molecular techniques and applications methods in molecular biology vol 666

Báo cáo khóa học: Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobility with pathogenic presenilin mutations pptx

Ngày tải lên : 16/03/2014, 16:20

... SY5Y and 5-day-old mouse brain were examined using 10% Tris/tricine gels with reducing Ó FEBS 2003 Analysis of mouse and human brain presenilin complex (Eur J Biochem 271) 381 Fig PEN-2 and APH-1 ... 10-kDa band in PS1 wild-type neural stem cells, E15 mouse brain and SY5Y cells Low-level expression was present in PS1 deﬁcient cells, adult mouse brain and in control and AD human brain cortex ... H.T., Tandon, A., Fraser, P.E & St George-Hyslop, P (2003) APH-1 interacts with mature and immature forms of presenilins and nicastrin and may play a role in maturation of presenilin.nicastrin complexes...

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Báo cáo khoa học: Analysis of the CK2-dependent phosphorylation of serine 13 in Cdc37 using a phospho-speciﬁc antibody and phospho-afﬁnity gel electrophoresis doc

Ngày tải lên : 30/03/2014, 03:20

... a kinase In many signal-transducing protein kinases, site-speciﬁc phosphorylation by an upstream protein kinase (a kinasekinase) activates them Before these activation steps, the protein kinases ... signaling protein kinases Cdc37 phosphorylation by CK2 and signaling kinases to Cdc37, we next investigated whether the CK2-phosphorylated form of Cdc37 associated with signaling protein kinases in ... Cdc37 band appearing within (Fig 8B, lane 8) Again, the band shift induced was direct, from the low-mobility to high-mobility bands, with no intermediate bands being detected Analysis of signaling...

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GEL ELECTROPHORESIS – PRINCIPLES AND BASICS pdf

Ngày tải lên : 28/06/2014, 15:20

... Staining: The most sensitive staining for protein is silver staining This involves soaking the gel in Ag NO3 which results in precipitation of metallic silver (Ag0) at the location of protein ... identification /finger printing of intact proteins or of proteins digested in gel slices Since gels used in biochemistry are chemically rather unreactive, they interact minimally with biomolecules during ... improve methods allowing unveil rotavirus co-infections in future studies These findings would be of interest in order to increase current knowledge about rotavirus evolution and determine the...

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Báo cáo khoa học: "Two-dimensional gel electrophoresis and immunoblot analysis of Neospora caninum tachyzoites" ppt

Ngày tải lên : 07/08/2014, 17:23

... rapid freezing and thawing times using liquid nitrogen, and continually disrupted using lysis buffer containing 9.5 M urea, 40 mM Tris-base, 4% (w/v) CHAPS, 1% (w/v) DTT, mM PMSF, and 0.5% (v/v) ... successive freezing and thawing, the intact tachyzoites remained were sonicated (XL-2020, Misonix, USA) at a low power for in ice slurry and continually disrupted using 9.5 M urea lysis buffer Finally, ... were incubated in 0.2% (w/v) silver nitrate containing 0.076% (v/v) formalin for 30 After incubation, it was rinsed with distilled water followed by adding the developing solution which contained...

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Báo cáo khoa học: "Effects of buffer system pH and tissue storage on starch gel electrophoresis of allozymes in three tropical tree species" pps

Ngày tải lên : 08/08/2014, 23:22

... many amino acid substitutions McLellan and Sherman (1991),using non-denaturating electrophoresis, reported that from 40- 57% of proteins differing by single amino acids can be separated on a single ... of the best methods for distinguishing among protein molecules (McLellan and Inouye, 1986) In the same way, isoelectric focusing (IEF), polyacrylamide gel electrophoresis (PAGE) and 2dimensional ... system, single zone of activity was evident in the species Staining intensity and resolving power were poor for T superba The R of ’s f a the observed zone were 0.36 and 0.40 for Using the H...

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Báo cáo khoa học: "The use of caspase inhibitors in pulsed-field gel electrophoresis may improve the estimation of radiation-induced DNA repair and apoptosis" pdf

Ngày tải lên : 09/08/2014, 09:20

... be inhibited by caspase inhibitors), and 3) reducing the interference of spontaneous breakage improves our ability to estimate DNA repair and to determine apoptosis intensity Methods Cell lines ... were approved in accordance with our own institutional guidelines for animal care and ethics When tumours reached 10 mm in size, they were excised, minced and incubated for 90 in Dubelcco’s Modified ... determine rejoining without the inference of the apoptosis Figure illustrates the effect of abrogating apoptosis in NP18 cells from a xenograft, showing clear differences between initial and residual...

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báo cáo khoa học: " Evaluation of protein pattern changes in roots and leaves of Zea mays plants in response to nitrate availability by two-dimensional gel electrophoresis analysis" pdf

Ngày tải lên : 12/08/2014, 03:21

... availability during the plant growth, in the protein profiles of both organs In order to obtain further information, in this work we investigated protein accumulation changes induced by nitrate in both ... total proteins, amino acids, reducing sugars and sucrose in roots (A, C, E and G) and leaves (B, D, F, and H) and chlorophyll content in leaves (I) of Zea mays plants, previously grown in the absence ... Healthcare) Determination of reducing sugars, sucrose, amino acids, total proteins and chlorophyll Reducing sugars, sucrose and amino acids were extracted by homogenizing frozen tissues in volumes of...

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Báo cáo y học: "Proteome changes of lungs artificially infected with H-PRRSV and N-PRRSV by two-dimensional fluorescence difference gel electrophoresis" pps

Ngày tải lên : 12/08/2014, 04:20

... against viral infection Others Rho GTPase activating protein 29 (PARG1, ARHGAP29), encoding for a protein-tyrosine phosphatase-associated Rho GTPase activating protein, is involved in signaling ... and utilize host cytoskeleton to promote viral infection like many other viruses[28,29] FLNA is an actin-binding and signal mediator scaffolding protein that crosslinks actin filaments and links ... control and experimentally infected pigs were processed routinely for haematoxylin and eosin (H&E) staining and were re-isolated of H-PRRSV and N-PRRSV viruses and then were identified by IFA and...

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Báo cáo y học: "Psoriasin, one of several new proteins identified in nasal lavage fluid from allergic and non-allergic individuals using 2-dimensional gel electrophoresis and mass spectrometry" potx

Ngày tải lên : 12/08/2014, 18:22

... Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Prolactin-inducible ... Prolactin-inducible protein Prolactin-inducible protein Prolactin-inducible protein Prolactin-inducible protein Cystatin S Cystatin S Cystatin SN Hemoglobin beta chain Hemoglobin beta chain Intersectin 2, (splice ... Lipocalin-1 Transthyretin Calgranulin B Psoriasin (S100A7) Psoriasin (S100A7) Galectin-3 Apolipoprotein A1 Apolipoprotein A1 Apolipoprotein A1 Alpha-2 glycoprotein 1, zink Alpha-2 glycoprotein 1, zink...

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Báo cáo y học: "Proteomic profiling of the mesenteric lymph after hemorrhagic shock: Differential gel electrophoresis and mass spectrometry analysis" pdf

Ngày tải lên : 13/08/2014, 13:20

... an increase in both the a and b chains of hemoglobin concurrent with the identification of haptoglobin, shown to decrease in PSML As haptoglobin is involved with hemoglobin degradation and in ... decreasing in PSML Ceruloplasmin is involved in iron transport across cells, and is involved in many cellular processes including iron metabolism [12] Its lowered abundance further points to ... triggered by thrombin into thrombopeptide A and B, uncovering the N-terminal polymerization sites on the a and b chains, allowing them to interact with the C-terminal g sites, and be cross-linked by factor...

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Tài liệu SEXUAL HEALTH EDUCATION A T SCHOOL AND A T HOME: ATTITUDES AND EXPERIENCES OF NEW BRUNSWICK PARENTS pptx

Ngày tải lên : 14/02/2014, 14:20

... parents provided suggestions regarding who should be involved in providing SHE (e.g., a public health nurse) and what training would be important (e.g., in- service training) Parent 5: Make sure the ... diseases, and abstinence in general terms only Overall, they had not discussed birth control methods and safer sex practices, sexual decisionmaking in dating relationships, and sexual pleasure and ... SHE should begin in grades K-3, 4-5, and 6-8 (33%, 32%, and 32% respectively) Thus, 65% of parents felt that SHE should begin in elementary school and 97% felt that it should begin in elementary...

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Tài liệu Báo cáo khoa học: "Different Structures for Evaluating Answers to Complex Questions: Pyramids Won’t Topple, and Neither Will Human Assessors" docx

Ngày tải lên : 20/02/2014, 12:20

... are meaningful depending on focus: individual instances or entire classes In tasks where it is important to correctly classify individual instances, microaveraging is more appropriate In tasks ... particular needs ﬁnding a particular nugget to be of interest Micro-averaging, on the other hand, assumes that some nuggets are inherently interesting, independent of the particular interests of users.2 ... stable and more discriminative than the original single-assessor evaluation, while requiring only a small additional cost in terms of human labor We have explored two different methods for combining...

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Gel electrophoresis

Ngày tải lên : 23/02/2014, 11:10

... protection and mop/wipe spill area Rinse with water No data Waste Disposal Method Can be disposed in the trash or down the sink Precautions to be Taken in Handling and Storing Avoid eye and skin contact ... mop/wipe spill area Rinse with water Waste Disposal Method Can be disposed in the trash or down the sink Precautions to be Taken in Handling and Storing Avoid eye and skin contact Soluble Other ... Limits No data Extinguishing Media Can be disposed in the trash or down the sink Precautions to be Taken in Handling and Storing Other Precautions Blue color, liquid, no odor Flash Point (Method Used)...

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Uniform t shirts and company uniforms

Ngày tải lên : 11/03/2014, 16:29

... must check the standard and also the status from the store before purchasing a uniform shirt stock from him The store should have a minimum of a good number of color designs and fashions of uniform ... any problem while selecting the designs and designs for his uniform shirt When the design continues to be selected, an order is positioned and also the package is shipped in the customer's doorstep ... organization authorities on the minimum order basis where the customer needs to buy a minimum of specific amount of t shirts It can be the maker to determine the minimum but limit for his or her...

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Gel Electrophoresis – Teacher Instructions

Ngày tải lên : 13/03/2014, 19:38

... hands when loading the gel; hold the sample-loading device in one hand and use your other hand and arm to support and stabilize the one doing the loading • When loading samples into the gel, be ... flask containing the solution (optional) • Bring the solution to a boil while stirring (or occasionally swirl the container) • Lower the heat and simmer (continue stirring or swirling) until ... Preparing scientific stains • 0.025 g powdered stain • 10 ml deionized or distilled water • ml glycerol • Combine and mix thoroughly Some stains may take a while to dissolve Stains are best kept in...

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T-Shirts and Suits A Guide to the Business of Creativity pptx

Ngày tải lên : 15/03/2014, 21:20

... attending training courses Think ‘learning’ rather than ‘training’ so as to open new possibilities for increasing knowledge and skills 23 Ideas in Action Peppered Sprout / Plastic Rhino Advertising ... and business best practice Written in an engaging and jargon-free style, the book offers inspiration and appropriate advice for all those involved in running or setting up a creative business ... about bringing together creativity and business as allies It’s about combining the best ideas of both ‘Tshirts’ and ‘Suits’ in the business of creativity, turning creative talent into income...

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Báo cáo khoa học: Investigating RNA polymerase II carboxyl-terminal domain (CTD) phosphorylation ˆ Benoıt Palancade and Olivier Bensaude pptx

Ngày tải lên : 17/03/2014, 10:20

... CTD-kinases in vitro CTD-kinases belong to three subclasses: cyclin-dependent kinases, mitogen-activated protein kinases and others (see Table and [15,17,18]) Two methods have been used to determine ... embryos inactivated for TBP [121], and both H5 and H14 staining are attenuated in mouse embryos following inactivation of MAT1, the CDK7 partner [122] These ﬁndings are consistent with an H14 staining ... staining attributed in part to initiating polymerases (productive or abortive) and an H5 staining of elongating polymerases Conclusion Determining the sites phosphorylated in vivo within RNAP II CTD...

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comparison of single and binary oxide moo3, tio2 and wo3 sol – gel gas sensors

Ngày tải lên : 19/03/2014, 16:47

... by increasing the adsorption and desorption rates [11] The grain, neck and grain boundary features also in uences the gas sensing properties It has been shown that the smaller grain size increases ... promising results to O3 as shown in Figs and Hence, MoO3 based sensors could be considered as promising candidates for O3 gas sensing Fig Oxygen dynamic response of TiO2 and MoO3 sensors operating ... sensing TiO2 had a negligible response to CO compared with WO3 and MoO3 MoO3 and WO3 showed promising CO and NO2 results MoO3 exhibited a high response to both CO and NO2 as shown in Fig The binary...

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T - engine and T - monitor

Ngày tải lên : 18/04/2014, 10:06

... thông tin bên alarm handler định alarm handler ID.Nếu thông số,thông tin tất alarm handler trình bày ID ID alarm handler STS Trạng thái alarm handler (STA = started) TIME Thời gian lại trước handler ... Get cyclic handler state Định dạng: ref_cyc [ ] Đặc tả: Trình bày thông tin bên cyclic handler định cyclic handler ID.Nếu thông số định,thông tin tất cyclic handler hiển thị ... Cyclic handler ID STS Trạng thái cyclic handler (STA = started) TIME Thời gian lại trước handler kế bắt đầu EXINF Thông tin mở rộng ref_alm Get alarm handler state Định dạng: ref_alm [

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why men don't listening and women don't reading

Ngày tải lên : 23/04/2014, 13:02

... www peaseinternational com Published in Great Britain by Orion Publishing Group Distributed in Australia and New Zealand by HarperCollins Ltd Imported to Great Britain by Pease Training International ... Men and Romance Some Surefire Romance Tips for Men Why Men Stop Touching and Talking Why Men Grope and Women Don't Is There Love in Springtime? How to Think Sexy Recreating Infatuation How to Find ... eat chocolate and go shopping Women criticise men for being insensitive, uncaring, not listening, not being warm and compassionate, not 20 talking, not giving enough love, not being committed...

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