Glycoprotein Methods and Protocols - P2

... T-antigen (β-d-Gal 1-3 GalNAc),but also to structures found within the backbone of oligosaccharides (β-d-Gal 1-3 /4GlcNAc) (19). Demonstration of PNA binding is not necessarily evidence of T-anti-gen ... mounting ,and its relative stability. There are, however, several other alternatives, such as 3-amino-9-ethylcarbazole (AEC), for use with horseradish peroxidase (HRP); 5-bromo-4-chloro-...

Ngày tải lên: 08/10/2012, 10:25

... al.for UDP-GlcNAc:GalNAc-Rβ1, 3-N-acetylglucosaminyltransferase (9). Actually, inin vitro experiments, these compounds inhibit the UDP-Gal:GalNAc-Rβ1,3-galac-tosyltransferase (8). GalNAcα-O-aryls ... with GalNAcα-O-aryl: The medium in ituma is added with aryl-N-acetyl-α-D-galactosaminide.c. Threonine-free medium (Life Technologies).d. Low-glucose Dulbecco’s minimal essential/H-16 medium (Gibc...

Ngày tải lên: 08/10/2012, 10:25

... H. (1986) Mucin synthesis: conversion of R 1- 1-3 Gal-R2to R 1- 1-3 (GlcNAcβ 1-6 )Gal-R 2and of R 1- 1-3 GalNAc-R2to R 1- 1-3 (GlcNAcβ 1-6 )GalNAc-R2by a β6-N-acetyl-glucosaminyltransferase in pig gastric mucosa. ... GlcNAcβ 1-6 (GlcNAcβ 1-3 ) GalNAcα-pnp, GlcNAcβ 1-3 Galβ 1-4 GlcNAc, GlcNAcβ 1-6 (GlcNAcβ 1-3 Galβ 1-3 ) GalNAcα-benzyl, GlcNAcβ 1-6 (GlcNAcβ...

Ngày tải lên: 08/10/2012, 10:25

... 100 mM IPTG; dissolve 23.8 mg isopropyl β-D-thiogalacto-pyranoside/mL in water. Filter sterilise. 2% X-gal: Dissolve 2% (w/v) 5-bromo-4-chloro-3-indoly-β-D-galactopyranoside in dimethyl formamide. ... β-galactosidase gene-coding region, allowing blue/white color screen-ing of clones with inserts in the presence of X-Gal and isopropyl-1-thiol-β-D-galactopyranoside (IPTG).Due to the large siz...

Ngày tải lên: 08/10/2012, 10:25

... expression bothin cell cultures (HT-29 MTX) and in tissues from various mucosae (trachea, bronchus,From :Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by: ... Hadzopuolou-Cladaras, M., Nolte, R. T., Atkinson, D., and Zannis V.J.(1986) The complete sequence and structural analysis of human apolipoprotein B-100relationship between apo...

Ngày tải lên: 08/10/2012, 10:25

... integrate and orientate this mapin the general physical map of 11p15.5 including HRAS, D11S150, and IFG2 refer-ence markers.From :Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: ... regions of mammalian genomes, inPulsed-Field Gel Electrophoresis : Protocols, Methods and Theories, Methods in Molecu-lar Biology, vol. 12 (Burmeister, M., and Vlan...

Ngày tải lên: 08/10/2012, 10:25

... 0.1% Tween-20, pH 9.5.5. Nitroblue tetrazolium (NBT) salt.6. 5-bromo-4-chloro-3-indolyl phosphate toluidinium (BCIP) salt.2.9.2. Fluorescent ISH (FISH)1. Buffer 1: 0.15 M NaCl, 0.1 M Tris-HCl, pH ... useof tandem repeat (TR) probes to quantitate mRNA levels, especially with those mucingenes that exhibit polymorphisms.From :Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and...

Ngày tải lên: 08/10/2012, 10:25

... TaqI, and MspI) reveal more than one set of bands. The large bands detected withPvuII correlate well in relative mobility with the PstI and HinfI bands, but the large bandsobserved with TaqI and ... Biochem. J. 293, 329–337.15. Guyonnet-Dupérat, V., Audié, J.-P., Debailleul, V., Laine, A., Buisine, M.-P., Galiègue-Zouitina, S., Pigny, P., Degand, P., Aubert, J.-P., and Porchet, N. (19...

Ngày tải lên: 08/10/2012, 10:25

... sequenceaMUC1VNTRp 1-4 0 PDTRPAPGSTAPPAHGVTSAPDTRPAPGSTAPPAHGVTSAp 1-2 4 PDTRPAPGSTAPPAHGVTSAPDTRp 5-2 0 PAPGSTAPPAHGVTSAp1 3-3 2 PAHGVTSAPDTRPAPGSTAPC-p1 3-3 2 (C)PAHGVTSAPDTRPAPGSTAPp 1-1 5 PDTRPAPGSTAPPAHA-p 1-1 5 APDTRPAPGSTAPPAHN-terminal ... peptide: the peptide Cp1 3-3 2, derived from MUC1 VNTR region (contain-ing an N-terminal cysteine to form dimers); peptides from N-...

Ngày tải lên: 08/10/2012, 10:25

... glycosidases, e.g., α-sialidase, - and β-galactosidase (Oxford Glycosciences,Abingdon, UK), - and β-N-acetylhexosaminidase (Boehringer Mannehim), α-fucosidase(Sigma), O-glycanase (Calbiochem -Novabiochem).2. ... Protein biotinylation reagent: δ-biotinyl-ε-aminocaproic-N-hydroxysuccinimide ester(BNHS) (Sigma).4. Carbohydrate biotinylation reagent: δ-biotinyl-ε-aminocaproic-acid hydra...

Ngày tải lên: 08/10/2012, 10:25