Week1 2 crystal structure

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... differences from the analogous structures of the ascomycete laccase (asco -laccase) MaL, thus giving a comprehensive view of the structure and function of asco-laccases Results and Discussion Overall structure ... Thornton JM (2003) Structural characterization and functional significance of transient protein–protein interactions J Mol Biol 325, 99 1–1 018 Crystal structure of a Thielavia arenaria laccase 54 Kiiskinen ... potentials of the enzyme and the substrate (DE °) [2 8–3 1] This study presents the crystal structure of a novel laccase (TaLcc1) from the ascomycete fungus Thielavia arenaria [32] The molecular mass of...

... A2 for main-chain atoms, 35.7 A2 for side-chain ˚ overall (3 244 atoms) For the pepatoms and 33.8 A tide, B factors are slightly higher than those for ˚ ˚ importin -a: 36.9 A2 for main-chain atoms, ... atoms, 40 .2 A2 for ˚ overall (62 total atoms) side-chain atoms and 38.7 A Electron density analysis Both the main-chain and side-chain atoms of the modelled CLIC4 peptide show a good fit to the electron ... fit, there are a large number (30) of atom -to- atom van der Waals’ contacts between the Tyr205 side chain and importin -a (Table 2) The numbers of contacts are comparable with those of the basic residues...

... illustrates the superimposition of ApeSOD with the cambialistic SOD from P shermanii The overall structures of these enzymes were similar; the rmsd of the 747 Ca atoms was ˚ 0.695 A ApeSOD eluted from the ... superoxide dismutases Biochim Biophys Acta 1804, 24 5–2 62 Crystal structure of SOD from A pernix K1 Sugio S, Hiraoka BY & Yamakura F (2000) Crystal structure of cambialistic superoxide dismutase from ... inhibition of the binding of the superoxide substrate and the other to product inhibition by hydrogen peroxide There are two mechanisms proposed for the reaction cycle of Mn ⁄ Fe-SOD: the 5-6-5 mechanism...

... substrate recognition For the superposition of the substrate- free PhyK with substrate- free ˚ AppA the Ca atoms are 2.41 A apart, whereas for the substrate- free PhyK and the substrate- loaded AppA ... B Fig Crystal structure of Klebsiella sp ASR1 phytase (A) Cartoon representation showing the two domains of PhyK, the a domain (orange) and the a ⁄ b domain (green) The disulfide bridges are represented ... the amino acid sequence of E coli glucose-1phosphatase (G1P) is related to AppA, the crystal structure suggests that phytate can bind to the active site of G1P only in an orientation with the...

... 14-48 form the small insertion domain that comprises a short a- helix and a three-stranded anti-parallel b-sheet; the remaining protein residues form the (a ⁄ b)8 barrel domain and a C-terminal extension ... would act as a regulatory link between glycolysis and NAD synthesis The structure of hACMSD in complex with DHAP may used for the design of potent and highly selective enzyme inhibitors that may ... (at 2.8 A) The DHAP aliphatic chain is sandwiched between Trp191 and Asp291, with its carbonyl oxygen contacting, at a ˚ distance of 2.7 A, both Asp291 and the solvent molecule w1 Finally, the...

... N-terminal regions of the a-subunits serves as catalytic domains, and the C-terminal regions of the a-subunits and the b-subunits act as regulatory domains [10,11] The regulatory domains of AK contain ... ª 2009 The Authors Journal compilation ª 2009 FEBS 3131 Crystal structures of regulatory subunit of Thr-sensitive aspartate kinase Potential factors involved in the high thermostability of TtAKb ... 2009 The Authors Journal compilation ª 2009 FEBS A Yoshida et al Crystal structures of regulatory subunit of Thr-sensitive aspartate kinase Table Thermostabilization factors Comparison of the...

... structure of the catalytic domain of DESC1 Scheme Domain organization of human DESC1 membrane region The extracellular part of DESC1 consists of a 120-amino acid SEA domain followed by the C-terminal ... growth factors and proteinase inhibitors Biol Chem 380, 473–483 Kataoka H, Uchino H, Asada Y, Hatakeyama K, Nabeshima K, Sumiyoshi A & Koono M (1997) Analy- Crystal structure of the catalytic domain ... that the SEA domain functions by orienting the active site cleft of DESC1 toward plasma and ⁄ or extracellular spaces and away from the cell surface and ⁄ or the extracellular matrix The SEA domain...

... References Ivanova N, Sorokin A, Anderson I, Galleron N, Candelon B, Kapatral V, Bhattacharyya A, Reznik G, Mikhailova N, Lapidus A et al (2003) Genome sequence of Bacillus cereus and comparative analysis ... of GAB catalysis have been identified to date [12] which are based either on a single, bifunctional GAB catalyst or on a GAB catalysts pair The available biochemical data on MshB and LpxC are ... 276–280 Handa N, Terada T, Kamewari Y, Hamana H, Tame JRH, Park S-Y, Kinoshita K, Ota M, Nakamura H, Kuramitsu S et al (2003) Crystal structure of the conserved protein TT1542 from Thermus thermophilus...

... protein crystallography Acta Crystallogr D Biol Crystallogr 50, 760–763 Navaza J (1994) Amore – an automated package for molecular replacement Acta Crystallogr A 50, 157–163 Jones TA, Zou JY, Cowan ... the accessible surface area of a monomer contributes to LlPDH dimer formation Whereas LlPDH and OaPDH have a ˚ buried surface area of  5500 A2 , TbPDH has a larger ˚ interface surface area of ... VA, Gerasimova TI, Kogan GL & Braslavskaya O (1976) Role of the pentose phosphate pathway in metabolism of Drosophila melanogaster elucidated by mutations affecting glucose 6-phosphate and 6-phosphogluconate...