Kinetics of Enzyme Reactions

Ngày tải lên: 01/10/2016, 21:37

... experiments and nucleophile binding studies we were able to obtain values for the kinetic constants for hydrolysis and synthesis of penicillin G, ampicillin and cephalexin Furthermore, insight into the ... the synthesis of ampicillin were obtained from fitting Eqn (8) to the data For synthesis of penicillin G, a value of 0.018 mM)1 for the slope of the cur...

Ngày tải lên: 08/03/2014, 08:20

... Burda, K & Strzalka, K (2000) A mathematical model describing kinetics of conversion of violaxanthin to zeaxanthin via intermediate antheraxanthin by the xanthophyll cycle enzyme violaxanthin ... monitored at 440 nm and quantitatively determined Analysis of de-epoxidation kinetics We have applied a new mathematical model [22] to analyse the kinetics of conver...

Ngày tải lên: 08/03/2014, 10:20

... mechanisms of autocatalytic zymogen activation and has been sufficiently justified [11,29–31] Previously, kinetic analyses of the reactions, whereby a zymogen is activated both intra- and intermolecularly ... for each of these mechanisms with the experimental results, these authors suggested a reaction mechanism including both intra- and intermolecular activation of...

Ngày tải lên: 16/03/2014, 18:20

... cứu ứng dụng chế phẩm enzyme pectinex để thu nhận đờng chức FOS từ dịch mía VậT LIệU V CứU PHƯƠNG PHáP NGHIÊN 2.1 Vật liệu Dịch mía giống mía chín trung bình F156 trồng vụ 2005 - 2006 đợc sử dụng ... hoạt tính 1ml dung dịch chế phẩm enzyme l 58,1 U/ml Do chế phảm pectin ex Ultra SP-L chứa enzyme -D frutofuranosidase, sử dụng chế phẩm n y để thu nhận...

Ngày tải lên: 29/08/2013, 08:16

... contributions and holds considerable promise for mechanism diagnosis and kinetic characterization of coupled chemical reactions 46 STUDY OF ELECTRODE REACTIONS AND INTERFACIAL PROPERTIES A B ... electron cloud of surface atoms 52 STUDY OF ELECTRODE REACTIONS AND INTERFACIAL PROPERTIES (a) (b) (c) Figure 2.15 STM image of 7.7 × 7.7-nm (a) and 2.65 ×...

Ngày tải lên: 25/10/2013, 08:20

... half-time for binding to the Characterization of the binding of PCSK9 to intact cells Fig Nonlinear regression and Scatchard analyses of binding- inhibition data (A) Inhibition of [125I]TC-PCSK9 (2.5 ... binding to HepG2 cells To determine whether the kinetics of binding of PCSK9 to HepG2 cells can be described as a simple bimolecular reaction, the...

Ngày tải lên: 14/02/2014, 14:20

... nigerooligosaccharides Efficient glucan synthesis, such that the accumulation of intermediate nigerooligosaccharides (DP = 2)6) is minimized, is explainable by the single-chain mechanism, whereby the ... minimal accumulation of intermediate nigerooligosaccharides is not achieved by continuous (or processive) elongation; it is, rather, achieved mainly through the enzymatic kinetic prop...

Ngày tải lên: 14/02/2014, 22:20

... questions of whether and how the enzymes structural dynamics inuence the kinetics of a-CT catalysis This was done by determining the changes in the global structural dynamics (DGHX) [38] for the ... the deprotonation and protonation rates of Ser195 thus reducing the kinetics of catalysis The results also suggest that the dynamics of the calc...

Ngày tải lên: 19/02/2014, 05:20

... activation of the glutaminase reaction and CTP synthesis was compared (Fig 5) In the absence of GTP, the rate of glutamine hydrolysis was signicantly higher than the rate of CTP synthesis so that the reactions ... (min) Fig Isothermal titration calorimetry of the glutaminase activity of L lactis CTP synthase (A) Enthalpogram showing the r...

Ngày tải lên: 21/02/2014, 15:20

... dose-dependent inhibition of Table Effects of reducing agents on DS-dependent inhibition of NAT1 Conditions % of control activitya NAT1 NAT1 NAT1 NAT1 NAT1 NAT1 NAT1 NAT1 0.5 1. 2 1. 3 1. 9 0.4 0.9 1. 4 1. 6 ... amine metabolism and carcinogenesis Mutat Res 65, 506–507 14 Grant DM, Blum M, Beer M & Meyer UA (19 91) Monomorphic and polymorphic human arylamine 15 16...

Ngày tải lên: 07/03/2014, 02:20

... understanding of the kinetic and chemical mechanism of GspS and TryS involved in the biosynthesis of glutathionylspermidine and trypanothione is crucial for the design of inhibitors against these ... compilation ê 2008 FEBS S L Oza et al Kinetics and inhibition of ATP-dependent ligases the inhibitor against each enzyme were determined using the following tight-...

Ngày tải lên: 07/03/2014, 04:20

... effect of Mg2+ on the dissociation rate of GTPcS and GDP from SEPT2 FEBS Journal 27 3 (20 06) 324 8– 326 0 ª 20 06 The Authors Journal compilation ª 20 06 FEBS 325 1 GTPase properties of human SEPT2 Y.-W ... D in the presence of 15 20 lm SEPT2 and mm MgCl2 and 100 lm of each FEBS Journal 27 3 (20 06) 324 8– 326 0 ª 20 06 The Authors Journal compilation ª 20 06 FEBS Y.-...

Ngày tải lên: 07/03/2014, 12:20

... removed by the yeast KEX2 protease (Pichia pastoris Expression Kit manual, Invitrogen, Groningen, the Netherlands) Cloning of elan and trappin-2 cDNA into pPIC9 Using the trappin-2 cDNA cloned into ... Measurement of kass and kdiss for the interaction of rec-elan or rec-trappin-2 with HNE and PR3 Linear inhibition curves were obtained when reacting increasing amount...

Ngày tải lên: 07/03/2014, 15:20

... in the programs and activities which the Institute operates The Effects of Inorganic Salts and Precipitated Calcium Carbonate Filler on the Hydrolysis Kinetics of Alkylketene Dimer Hui JiangandYulin ... after the hydrolysis reaction if the reaction solutionwas unbuffered Sincethe AKD hydrolysis rate is very sensitiveto the solution pH, the e...

Ngày tải lên: 09/03/2014, 01:20