Kinetics of Enzyme Reactions
Kinetics of Enzyme Reactions
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Báo cáo khoa học: Kinetics of enzyme acylation and deacylation in the penicillin acylase-catalyzed synthesis of b-lactam antibiotics pptx
... experiments and nucleophile binding studies we were able to obtain values for the kinetic constants for hydrolysis and synthesis of penicillin G, ampicillin and cephalexin Furthermore, insight into the ... the synthesis of ampicillin were obtained from fitting Eqn (8) to the data For synthesis of penicillin G, a value of 0.018 mM)1 for the slope of the curve was obtained, but due to the almost linear ... amides and the high affinity of the enzyme for the product of synthesis increase a and hence reduce the yield in synthesis reactions Bruggink, A., Roos, E.R & de Vroom, E (1998) Penicillin acylase in...
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Báo cáo khoa học: Kinetics of violaxanthin de-epoxidation by violaxanthin de-epoxidase, a xanthophyll cycle enzyme, is regulated by membrane fluidity in model lipid bilayers pptx
... Burda, K & Strzalka, K (2000) A mathematical model describing kinetics of conversion of violaxanthin to zeaxanthin via intermediate antheraxanthin by the xanthophyll cycle enzyme violaxanthin ... monitored at 440 nm and quantitatively determined Analysis of de-epoxidation kinetics We have applied a new mathematical model [22] to analyse the kinetics of conversion of violaxanthin to zeaxanthin ... antheraxanthin to zeaxanthin On the basis of our results and literature data [15,22], we postulate that changes in membrane fluidity may play an important role in regulation of the violaxanthin...
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Báo cáo khoa học: Kinetics of intra- and intermolecular zymogen activation with formation of an enzyme–zymogen complex ppt
... mechanisms of autocatalytic zymogen activation and has been sufficiently justified [11,29–31] Previously, kinetic analyses of the reactions, whereby a zymogen is activated both intra- and intermolecularly ... for each of these mechanisms with the experimental results, these authors suggested a reaction mechanism including both intra- and intermolecular activation of the zymogen by the action of the ... route of intramolecular activation of the zymogen into the active enzyme, E, and one or more peptides represented by W [route (a), Scheme 3] [12,22,25–27] and a route of autocatalytic activation of...
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ứng dụng công nghệ enzyme để thu nhận đ-ờng chức năng fructooligosaccharide (FOS) từ dịch mía Application of enzyme technology for fructooligosaccharides (FOS) production
... cứu ứng dụng chế phẩm enzyme pectinex để thu nhận đờng chức FOS từ dịch mía VậT LIệU V CứU PHƯƠNG PHáP NGHIÊN 2.1 Vật liệu Dịch mía giống mía chín trung bình F156 trồng vụ 2005 - 2006 đợc sử dụng ... hoạt tính 1ml dung dịch chế phẩm enzyme l 58,1 U/ml Do chế phảm pectin ex Ultra SP-L chứa enzyme -D frutofuranosidase, sử dụng chế phẩm n y để thu nhận fructooligosaccharide (FOS) từ đờng saccharose ... hoạt tính enzyme -D fructofuranosidase chế phẩm pectinex Ultra SP-L v h m lợng đờng saccharose dịch mía Để có định hớng sử dụng chế phẩm enzyme pectinex-Ultra SP-L, hoạt tính enzyme -D frutofuranosidase...
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35768 Study of Electrode Reactions and Interfacial Properties
... contributions and holds considerable promise for mechanism diagnosis and kinetic characterization of coupled chemical reactions 46 STUDY OF ELECTRODE REACTIONS AND INTERFACIAL PROPERTIES A B ... electron cloud of surface atoms 52 STUDY OF ELECTRODE REACTIONS AND INTERFACIAL PROPERTIES (a) (b) (c) Figure 2.15 STM image of 7.7 × 7.7-nm (a) and 2.65 × 2.65-nm (b) sections of an ethanethiolate ... couple during the forward and reversed scans (b,d), and to the achievement of a zero-reactant surface concentration (c) 32 STUDY OF ELECTRODE REACTIONS AND INTERFACIAL PROPERTIES exhibits a t−1/2...
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Tài liệu Báo cáo khoa học: Affinity and kinetics of proprotein convertase subtilisin ⁄ kexin type 9 binding to low-density lipoprotein receptors on HepG2 cells docx
... half-time for binding to the Characterization of the binding of PCSK9 to intact cells Fig Nonlinear regression and Scatchard analyses of binding- inhibition data (A) Inhibition of [125I]TC-PCSK9 (2.5 ... binding to HepG2 cells To determine whether the kinetics of binding of PCSK9 to HepG2 cells can be described as a simple bimolecular reaction, the kinetics of [125I]TC-PCSK9 and [125I]TC-PCSK9-D374Y ... [125I]TC-PCSK9 binding by unlabeled wild -type PCSK9 (C) Inhibition of [125I]TCPCSK9-D374Y (1 lgÆmL)1) binding to HepG2 cells (9. 2 9. 8 · 105) by increasing concentrations of unlabeled PCSK9-D374Y Error...
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Tài liệu Báo cáo khoa học: Kinetics of dextran-independent a-(1 fi 3)-glucan synthesis by Streptococcus sobrinus glucosyltransferase I pdf
... nigerooligosaccharides Efficient glucan synthesis, such that the accumulation of intermediate nigerooligosaccharides (DP = 2)6) is minimized, is explainable by the single-chain mechanism, whereby the ... minimal accumulation of intermediate nigerooligosaccharides is not achieved by continuous (or processive) elongation; it is, rather, achieved mainly through the enzymatic kinetic properties of ... relationship between the kinetic properties of GTF and the time course of saccharide production Owing to GTF’s kinetic property of improving activity in the presence of nigerooligosaccharides with...
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Tài liệu Báo cáo khoa học: Influence of modulated structural dynamics on the kinetics of a-chymotrypsin catalysis Insights through chemical glycosylation, molecular dynamics and domain motion analysis pptx
... questions of whether and how the enzymes structural dynamics inuence the kinetics of a-CT catalysis This was done by determining the changes in the global structural dynamics (DGHX) [38] for the ... the deprotonation and protonation rates of Ser195 thus reducing the kinetics of catalysis The results also suggest that the dynamics of the calcium binding site in this structural class of proteins ... between the enzymes conformational dynamics and active-site chemistry through domain motions [17,18,21,78] Conclusions In this article, we further demonstrate the value of chemical glycosylation as...
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Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx
... activation of the glutaminase reaction and CTP synthesis was compared (Fig 5) In the absence of GTP, the rate of glutamine hydrolysis was signicantly higher than the rate of CTP synthesis so that the reactions ... (min) Fig Isothermal titration calorimetry of the glutaminase activity of L lactis CTP synthase (A) Enthalpogram showing the recording of steady-state rates for the hydrolysis of glutamine at increasing ... of the glutaminase reaction of CTP synthase from L lactis in order to distinguish between the effects of GTP on the glutaminase reaction and the CTP synthesis reaction The results from this work...
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Báo cáo khoa học: Mechanisms and kinetics of human arylamine N-acetyltransferase 1 inhibition by disulfiram potx
... dose-dependent inhibition of Table Effects of reducing agents on DS-dependent inhibition of NAT1 Conditions % of control activitya NAT1 NAT1 NAT1 NAT1 NAT1 NAT1 NAT1 NAT1 0.5 1. 2 1. 3 1. 9 0.4 0.9 1. 4 1. 6 ... amine metabolism and carcinogenesis Mutat Res 65, 506–507 14 Grant DM, Blum M, Beer M & Meyer UA (19 91) Monomorphic and polymorphic human arylamine 15 16 17 18 19 20 21 22 23 24 25 26 N-acetyltransferases: ... constant for the inhibition of NAT1 by DS (ki) was deduced from the slope of kobs as a function of the DS concentration [DS], and was · 10 4 m )1 min )1 The order of the reaction of DS with NAT1 (n) can...
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Báo cáo khoa học: ATP-dependent ligases in trypanothione biosynthesis – kinetics of catalysis and inhibition by phosphinic acid pseudopeptides doc
... understanding of the kinetic and chemical mechanism of GspS and TryS involved in the biosynthesis of glutathionylspermidine and trypanothione is crucial for the design of inhibitors against these ... compilation ê 2008 FEBS S L Oza et al Kinetics and inhibition of ATP-dependent ligases the inhibitor against each enzyme were determined using the following tight-binding inhibition equation [41] (Eqn ... an insertion of 17 amino acids in the amidase domain and two in the synthetase domain (one of 14 amino acids and the Fig Conservation of key functional residues identied for EcGspS in CfGspS and...
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Báo cáo khoa học: GTP binding and hydrolysis kinetics of human septin 2 pot
... effect of Mg2+ on the dissociation rate of GTPcS and GDP from SEPT2 FEBS Journal 27 3 (20 06) 324 8– 326 0 ª 20 06 The Authors Journal compilation ª 20 06 FEBS 325 1 GTPase properties of human SEPT2 Y.-W ... D in the presence of 15 20 lm SEPT2 and mm MgCl2 and 100 lm of each FEBS Journal 27 3 (20 06) 324 8– 326 0 ª 20 06 The Authors Journal compilation ª 20 06 FEBS Y.-W Huang et al GTP, GTPcS or GDP for ... Wang Z & Zheng Y (20 00) The role of Mg2+ cofactor in the guanine nucleotide exchange and GTP hydrolysis reactions of Rho family GTP- binding proteins J Biol Chem 27 5, 25 299 25 307 326 0 35 Zhao J,...
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Báo cáo khoa học: Kinetics of the inhibition of neutrophil proteinases by recombinant elafin and pre-elafin (trappin-2) expressed in Pichia pastoris ppt
... removed by the yeast KEX2 protease (Pichia pastoris Expression Kit manual, Invitrogen, Groningen, the Netherlands) Cloning of elan and trappin-2 cDNA into pPIC9 Using the trappin-2 cDNA cloned into ... Measurement of kass and kdiss for the interaction of rec-elan or rec-trappin-2 with HNE and PR3 Linear inhibition curves were obtained when reacting increasing amounts of each inhibitor with HNE and ... signicantly the bioavailability of such an inhibitor at the site of inammation, e.g in the case of therapeutic administration, as well as providing a source of inhibitory elan Knowledge of the kinetic...
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The Effects of Inorganic Salts and Precipitated Calcium Carbonate Filler on the Hydrolysis Kinetics of Alkylketene Dimer docx
... in the programs and activities which the Institute operates The Effects of Inorganic Salts and Precipitated Calcium Carbonate Filler on the Hydrolysis Kinetics of Alkylketene Dimer Hui JiangandYulin ... after the hydrolysis reaction if the reaction solutionwas unbuffered Sincethe AKD hydrolysis rate is very sensitiveto the solution pH, the effect of buffer solutionsand PCC on the hydrolysisof AKD ... obviouseffect on the hydrolysisof AKD; thus, the acceleration of the hydrolysisrate in buffer solutionsmay be mainly contributedto anionicions HzPOd’, C032-andHC03’ To further study the anionic effect, the...
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