kinetics of heterogeneous catalytic reactions

... 17:27:45 Catalytic fast pyrolysis of biomass: the reactions of water and aromatic intermediates produces phenols Green Chemistry Page of 16 View Article Online a reduction in formation of deoxygenated ... during the pyrolysis of the lignin component of biomass; phenols are also formed by reaction of water molecules with aromatic intermediates formed during the transformation of all of the pyrolysis ... measurement of products and facilitated determination of the impact of steam during catalytic upgrading, complemented by a tandem micropyrolyzer connected to a GCMS for identification of the products...

Ngày tải lên: 09/10/2015, 14:39

... deuteriation of products of hydrogenation reactions (see also 3.1.3 and 3.1.4) Hydration of olefins, alkynes and nitriles calls explicitely for the use of aqueous solvents Indeed, one of the earliest ... hydrocyanation of 1,3-butediene) Not surprisingly, already one of the first Rhone Poluenc patents on the use of water soluble complexes of TPPTS described the Ni-catalyzed hydration of butadiene ... processes have been used as test reactions in developing microreactors for dynamic, high throughput screening of fluid/liquid molecular catalysis [45] Miscellaneous catalytic reactions in aqueous media...

Ngày tải lên: 23/12/2013, 07:19

... able to track the chemical dynamics of simple reactions (e.g elementary reactions) and complicated chemical reactions (e.g heterogeneous chemical reaction, and reactions involving catalysts) Compared ... individually after the addition of acrylonitrile (shown in Fig 2) demonstrate the wealth of valuable information provided about ongoing chemical reactions by EESI-MS At the beginning of the reaction, the ... Monitoring of organic chemical reactions using EESI-MS Figure Mass spectra of the Michael addition reaction recorded at 20, 60 and 300 min, respectively Inserts: molecular structures of PEA, PEAP...

Ngày tải lên: 14/02/2014, 08:20

... characteristics of [125I]TC-PCSK9 binding to HepG2 cells To determine whether the kinetics of binding of PCSK9 to HepG2 cells can be described as a simple bimolecular reaction, the kinetics of [125I]TC-PCSK9 ... [koff] of 0.0009 ± 0.00012 min)1 (n = 3), which corresponds to a half-time of dissociation of 770 Lowering the pH of dissociation medium to 6.2 also led to monophasic and slow dissociation of ... Characterization of the binding of PCSK9 to intact cells S A Mousavi et al As the gain -of- function D374Y mutation is localized in the LDLR-binding region of PCSK9, the enhanced binding of [125I]TC-PCSK9-D374Y...

Ngày tải lên: 14/02/2014, 14:20

... kinetics of the acceptor reactions appeared to be similar between GS and GSGB Kinetic simulation of GTF glucan synthesis To explain the time course of saccharide production during the synthesis of ... the absence of dextran GTF-I was assumed to be capable of catalyzing the following reactions: (a) the hydrolysis of sucrose to glucose and fructose (reaction 1); (b) the transfer of glucose residues ... the presence of increasing DP may be a common feature of glucansucrase Additional studies are required to elucidate the dextran-dependent synthesis of glucan As the affinity of the GBd of GTF-I for...

Ngày tải lên: 14/02/2014, 22:20

... Max-Planck-Insitute of Biochemistry Journal compilation ª 2007 FEBS O J P Kyrieleis et al Crystal structure of the catalytic domain of DESC1 Fig Structure-based sequence alignment of the human DESC1 catalytic ... mediate interaction of the two b-barrels, formation of the catalytic machinery and structures required for binding of the main chain of the substrate peptide and proper positioning of the scissile ... Max-Planck-Insitute of Biochemistry Journal compilation ª 2007 FEBS 2149 Crystal structure of the catalytic domain of DESC1 O J P Kyrieleis et al Fig Stereo ribbon representation of DESC1 in complex...

Ngày tải lên: 19/02/2014, 00:20

... on the kinetics of enzymatic catalysis from both an experimental and theoretical perspective Changes in the kinetics of a-CT catalysis upon chemical glycosylation The catalytic behavior of a-CT ... methodology to the study of the kinetics of catalysis by the serine protease a-CT we were able to statistically correlate the structurally dynamic behavior of the enzyme with its kinetics of catalysis From ... cutoff distance (rc ẳ 6.0 A) The dynamics of the resulting network are then dened by the Ni ã Nj Kirchhoff connectivity matrix of interresidue contacts (G) where the off diagonal elements of...

Ngày tải lên: 19/02/2014, 05:20

... value (e) of 1.49 · 105 M)1Æcm)1 at 407 nm for MP8 In the case of BSA, mol of MP8 were bound per mol of protein, whereas in the case of KLH, 22 mol of MP8 were bound per 100 000 g of protein ... method [12] The purity of the sample was greater than 97%, based on MALDI-TOF mass spectrometry Preparation of monoclonal antibodies Scheme Mechanisms of oxygen transfer reactions catalyzed by ... function of time, between 350 and 550 nm Kinetic data were then obtained by measuring the absorbance at the maximum of absorption of the MP8–Fe(II)–RNO complex, as a function of time S-oxidation of...

Ngày tải lên: 19/02/2014, 12:20

... oligomeric forms of the enzyme will not interfere with the interpretation of the data In this research, we have analyzed the steady state kinetics of the glutaminase reaction of CTP synthase from ... time are derived from the heat of dilution of glutamine into the reaction cell (B) The heat generated by hydrolysis of 0.15 lM of glutamine used to determine DH of the reaction ể FEBS 2002 4774 ... activation of the glutaminase reaction and CTP synthesis was compared (Fig 5) In the absence of GTP, the rate of glutamine hydrolysis was signicantly higher than the rate of CTP synthesis so that the reactions...

Ngày tải lên: 21/02/2014, 15:20

... phosphorylation of a microarray of 1176 peptides in the presence of [c-33P]ATP The magnified inset shows an example of the results obtained after detection by phosphorimaging (B) Peptide sequences of the ... awaits the identification of the endogenous stimulus of PASKIN catalytic activity Autophosphorylation of recombinant PASKIN is activated by phospholipids A possible mechanism of PASKIN activation ... GST-tag (C) Immunoblot analysis of the phosphorylation status of p70S6K and S6 in Paskin+ ⁄ + and Paskin) ⁄ ) MEFs (D) Immunoblot analysis of the phosphorylation status of p70S6K and S6 in S6K1) ⁄...

Ngày tải lên: 06/03/2014, 00:21

... concentrations of the non-physiological reductant dithiothreitol These data suggest that the DS-dependent inhibition of NAT1 is unlikely to be a result of the formation of a mixed disulfide, but rather of ... constant for the inhibition of NAT1 by DS (ki) was deduced from the slope of kobs as a function of the DS concentration [DS], and was · 104 m)1Æmin)1 The order of the reaction of DS with NAT1 (n) can ... modification of the active site catalytic cysteine residue of NAT1 by DS Discussion DS is an inhibitor of aldehyde dehydrogenase and is currently being used clinically for the treatment of alcoholism...

Ngày tải lên: 07/03/2014, 02:20

... addition of a t peptide at the C-terminus of green fluorescent protein or alkaline phosphatase allowed the formation of tetramers associated with an N-terminal fragment of ColQ [17] However, the catalytic ... and of the t peptides For this purpose, we constructed chimeric proteins, in which we associated the catalytic domain of each enzyme with the t peptide of the other For convenience, the large catalytic ... AChE and BChE The T variants of human AChE and human BChE are composed of a catalytic domain of approximately 500 residues, followed by small C-terminal t peptides of 40 and 41 residues, respectively...

Ngày tải lên: 07/03/2014, 03:20

... phosphinic acid derivatives of GSH as moderate Kinetics and inhibition of ATP-dependent ligases inhibitors of CfGspS [38] The most active of these was a phosphonic analogue of GSH (c-l-Glu-l-Leu-GlyP), ... Results Initial velocity analysis of the kinetic mechanism of GspS A matrix of kinetic data was collected in order to determine the kinetic mechanism of GspS Six families of kinetic data were generated ... the concentration of phosphinate and initiating with enzyme Modality of inhibition The mode of inhibition of the slow-binding phosphinate was determined by examining the effect of varying each...

Ngày tải lên: 07/03/2014, 04:20

... domain of full-length RICH protein relatively close to its Fig Structure of goldfish RICH catalytic domain (A) Stereo view of the backbone superposition of 10 lowest energy structures of the RICH catalytic ... Solution structure of the RICH catalytic domain G Kozlov et al Results Structure of the RICH catalytic domain We determined the structure of the 24 kDa catalytic fragment of goldfish RICH protein (Fig ... RICH catalytic fragment Secondary structure and the two catalytic motifs (*) are shown (B) Representation of flexibility in the solution structure of the RICH catalytic domain The width of the...

Ngày tải lên: 07/03/2014, 10:20

... mutagenesis of Ser218 to Ala, as similar analysis of the mutated protein showed no evidence of phosphorylation [26] Residue S218 is detected by a variety of phosphorylation prediction software to ... Kd of SEPT2 is 0.28 lm and that of GDP is 1.7 lm in the presence of physiological Mg2+ concentrations These values are about an order of magnitude lower than those observed for complexes of yeast ... completion of most of the studies described herein Given the conserved nature of this residue we therefore mutated the residue back to arginine and found that none of the properties of the protein...

Ngày tải lên: 07/03/2014, 12:20

... elan-immunoreactive material Fig Evolution of rec-trappin-2 production by Pichia pastoris as a function of the duration of fermentation Aliquots of concentrated supernatants of rec-trappin-2-secreting P pastoris ... Simon, S.R (2001) Kinetics of the Inhibition of Proteinase by Elan Am J Respir Cell Mol Biol 24, 8389 25 Bieth, J.G (1995) Theoretical and practical aspects of proteinase inhibition kinetics Methods ... before measurement of the residual enzymatic activity by addition of 10 lL of a 100 mM Suc(Ala)3-p-NA stock solution Product release was continuously recorded until a constant rate of paranitroaniline...

Ngày tải lên: 07/03/2014, 15:20

... [N] and the results of the inhibition studies show that, despite of the high rate of hydrolysis of the acyl-enzyme, tight binding of the nucleophile and displacement of the catalytic H2O molecule ... analysis of penicillin acylase (Eur J Biochem 270) 3681 Fig Inhibition of NIPGB hydrolysis by 7-ADCA Lineweaver–Burk plot of the effect of various concentrations of 7-ADCA on the conversion of NIPGB ... reactive ester of NIPGB in kinetic experiments, failed because of the high rates of spontaneous hydrolysis of this compound The only substrate of PA for which the steady-state rate of hydrolysis...

Ngày tải lên: 08/03/2014, 08:20

... aggregates offers new possibilities in the investigation of the kinetic parameters and mechanism of violaxanthin de-epoxidation One of the advantages of such system is the defined orientation of violaxanthin ... [22] to analyse the kinetics of conversion of violaxanthin to zeaxanthin The model allowed us to follow independently the kinetics of the two de-epoxidation steps: the conversion of violaxanthin ... application of a mathematical model of de-epoxidation for the analysis of the obtained results provide important information on the influence of membrane physical properties, kinetic parameters of violaxanthin...

Ngày tải lên: 08/03/2014, 10:20

... little direct effect on the activity of the enzyme The function of this unique C-terminal domain remains unknown Overall structure Crystals of the catalytic domain of hTRXL (hTRXL-N) were obtained ... hTRXL-N may account for the formation of a monomer, instead of a dimer in the case of TRX Furthermore, the loss of intermolecular disulfide-bonds and the disbandment of the hydrophobic patch may also ... consisting of b sheets shows little difference with other TRX (Fig 3B) Active site The location of the active site in all of the known thioredoxin structures is identical It includes the end of b-2,...

Ngày tải lên: 08/03/2014, 22:20

... Beta-ketoEsterFormationDuring the Sizingof Paperwith AlkylketeneDimers”, TappiJ., 70(4):135-139(1987) MARTON, J., “PracticalAspectsof Alkaline Sizingon Kinetics of Alkyl Ketene Dimer Reactions: Hydrolysis of Alkyl KeteneDime?, ... have a significant catalytic effect on the hydrolysis of AKD, andthe mechanism this catalyticeffect is discussed of Keywords: Alkylketenedimer, salt concentration, sizing ,kinetics, hydrolysis ... detrimentalmaterialson of the AKD hvdrolvsis kinetics have not been reported This paper focuses on the J d hydrolysis kinetics of AKD at different wet-end conditions, particularly at different concentrationsof salts...

Ngày tải lên: 09/03/2014, 01:20