introductory econometrics a modern approach
ENZYME KINETICS A MODERN APPROACH – PART 1 pps
... / 12 7 11 TRANSIENT PHASES OF ENZYMATIC REACTIONS 11 .1 Rapid Reaction Techniques / 13 0 11 .2 Reaction Mechanisms / 13 2 12 9 CONTENTS xi 11 .2 .1 Early Stages of the Reaction / 13 4 11 .2.2 Late Stages ... 12 2 10 .1. 1 Interfacial Binding / 12 2 10 .1. 2 Interfacial Catalysis / 12 3 10 .2 Determination of Interfacial Area per Unit Volume / 12 5 10 .3 Determination of Sa...
ENZYME KINETICS A MODERN APPROACH – PART 2 pptx
... undissociated acid HA and conjugate base A at constant ionic strength Thus, kc reduces to kc = kHA [HA] + kA− [A ] (1.80) which can be expressed as kc = kHA [HA] + kA− Ka [HA] Ka − + ] = kHA + kA ... conjugate base–catalyzed reaction 22 TOOLS AND TECHNIQUES OF KINETIC ANALYSIS The overall rate of this acid/base-catalyzed reaction (v) corresponds to the summation of each of these individual...
ENZYME KINETICS A MODERN APPROACH – PART 3 pps
... model A steady-state approximation can also be used to obtain the rate equation for an enzyme- catalyzed reaction 3. 2.2 Steady-State Model The main assumption made in the steady-state approximation ... Michaelis–Menten model to the experimental data allowed for rapid and accurate determination of the catalytic parameters of this enzyme- catalyzed reaction The estimates of Vmax and Km ,...
ENZYME KINETICS A MODERN APPROACH – PART 4 pptx
... is appropriate When more than two values are compared, a one-way analysis of variance (ANOVA), TABLE 4. 2 Rate of Hydration of Fumarate to Malate by Fumarase at various Substrate Concentrationsa ... where Vmax and Ks correspond, respectively, to apparent enzyme maximum velocity and apparent enzyme substrate dissociation constant at a ∗ ∗ particular pH For the model above, Vmax = Vmax...
ENZYME KINETICS A MODERN APPROACH – PART 5 pptx
... complexes EAB (K AB and K BA ), and the enzyme mass balance are, respectively, v = kcat [EAB] [E] [A] [E][B] A B Ks = Ks = [EA] [EB] [EB] [A] [EA][B] K BA = K AB = [EBA] [EAB] [ET ] = [E] + [EA] + [EB] ... cooperativity, which can have noninteger values Estimates of the parameters k and n are obtained using standard nonlinear regression procedures available in most modern graphical soft...
ENZYME KINETICS A MODERN APPROACH – PART 6 pptx
... concentration in this treatment In determination of the catalytic parameters of an enzyme- catalyzed interfacial reaction, increasing amounts of substrate are added to a solution 121 122 INTERFACIAL ENZYMES ... for an interfacial enzyme various parameters in Eq (10.9) on the velocity of a reaction catalyzed by an interfacial enzyme As the enzyme interface dissociation constant incre...
ENZYME KINETICS A MODERN APPROACH – PART 7 ppsx
... the apparent rate constants for a number of relaxation reactions are summarized in Table 11.1 CHAPTER 12 CHARACTERIZATION OF ENZYME STABILITY In many enzyme- related studies, an index of enzyme ... enzyme 12.3 EXAMPLE For the kinetic characterization of enzyme stability, enzyme solutions are incubated at a particular temperature and aliquots removed at the appropriate times E...
ENZYME KINETICS A MODERN APPROACH – PART 8 ppt
... (for examples see Hlasta et al., 1996; Groutas et al., 19 98; Macchia et al., 2000; Clemente et al., 2001) Amino acid analysis of both native and inactivated enzyme can identify which amino acid ... Robinson, and A Krantz, J Am Chem Soc 1 08, 5 58 9–5 597 (1 986 ) REFERENCES 173 Swar´ n, P., I Massova, J R Bellettini, A Bulychev, L Maveyraud, L P Kotra, e M J Miller, S Mobashery, an...
ENZYME KINETICS A MODERN APPROACH – PART 9 docx
... the mean of a minimum of two determinations ± standard deviation b ss1, substrate consisting of the peptide lysine–proline–alanine–glutamic acid–phenylalanine– phenylalanine (NO2 )–alanine–leucine ... Baratti, G Buono, and C Triantaphylides, 198 7 Lipase-catalyzed reactions in organic media: competition and applications Biochim Biophys Acta 91 1: 11 7–1 20 Fersht, A. , Enzyme Structur...
ENZYME KINETICS A MODERN APPROACH – PART 10 pps
... basic postulates of, 10 3–1 05 dissociation constants in, 10 6–1 07 generalized, 10 7–1 09 interaction factors in, 10 5–1 06 for multisite and cooperative enzymes, 10 3–1 09 Sequential mechanisms, 9 0–9 1 ... versus, 5 1–5 2 Integral method, reaction order and rate determination via, 1 3–1 4 Integrated rate equations, 4–1 2 Interaction factors, with cooperative enz...
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