Structure and mechanism studies of sugar epimerase from dict

Structure and mechanism studies of sugar epimerase from dictyoglomus turgidum

... Structure and Mechanism Studies of Sugar Epimerase fromDictyoglomus turgidum A Dissertationsubmitted to the Department of Advanced Technology Fusion and the Graduate School of Konkuk ... School of Konkuk University TABLE OF CONTENTS List of Tables v List of Figures vi Abstract ix Chapter Structure and mechanism studies of sugar epimerase from D...

Ngày tải lên : 20/05/2016, 21:08

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FUNCTION AND MECHANISM STUDIES OF TRPV4 IN BREAST CANCER METASTASIS

... Upregulation of TRPV4 protein and mRNA across the BCM model 3.1.4 Upregulation of TRPV4 in invasive human breast cancer cell lines and tissues 37 37 42 TRPV4 is a positive regulator of breast cancer metastasis ... roles and modes of actions in breast cancer metastasis remain obscure We hypothesize that TRPV4 is a positive regulator in breast canc...

Ngày tải lên : 02/10/2015, 17:15

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Báo cáo khoa học: Structure and mechanism of the ThDP-dependent benzaldehyde lyase from Pseudomonas ﬂuorescens potx

... with the gene of the benzaldehyde lyase and for helpful discussions, the teams of the Swiss Light Source (Villigen ⁄ CH) and of the EMBL outstation Hamburg for their help with data collection and ... involved in binding of the cofactor ThDP are located at the C-terminal ends of the b-strands of Dom-c (diphosphates and Mg2+) and of Dom-a¢ of a neig...

Ngày tải lên : 07/03/2014, 12:20

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Báo cáo khoa học: Structural studies of thymidine kinases from Bacillus anthracis and Bacillus cereus provide insights into quaternary structure and conformational changes upon substrate binding pot

... this study, we describe the structures of two homologues, TKs from B anthracis and B cereus, in complex with substrate dT and phosphate donormimicking dTTP Because of the high sequence identity ... expression and puriﬁcation of human thymidine kinase 1: quaternary structure, stability, and kinetics Protein Expr Purif 47, 506–515 20 Black ME & Hruby DE (1990) Quatern...

Ngày tải lên : 23/03/2014, 09:21

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Báo cáo khoa học: Crystal structure determination and inhibition studies of a novel xylanase and a-amylase inhibitor protein (XAIP) from Scadoxus multiﬂorus pot

... kDa, a second peak of 30 kDa and a third peak of about 20 kDa A similar gel ﬁltration experiment was also carried out for the complex of XAIP with a- amylase A mixture of XAIP and a- amylase was ... observations indicate that XAIP associates with GH11 xylanase and GH13 a- amylase, as well as with both xylanase and a- amylase simultaneously Tissue distribution...

Ngày tải lên : 29/03/2014, 09:20

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Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx

... robust synthesis of pantothenate from low amounts of pantoate [9] Here, we report the results of the structural studies of the pantoate- bound form of the N-terminal domain of E coli PS (nPS) The structure ... [13] Details of the structural basis for the biochemically observed inhibition of plant PS by pantoate, as well as of the alleviation of...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: Comparative studies of endonuclease I from cold-adapted Vibrio salmonicida and mesophilic Vibrio cholerae docx

... and plasmid were analyzed using linearized pBAD ⁄ gIII plasmid, linearized and denatured plasmid and intact plasmid The pBAD ⁄ gIII plasmid was linearized using SalI and denatured by incubation ... Biochemical Adaptation Princeton University Press, Princeton, NJ Wu SI, Lo SK, Shao CP, Tsai HW & Hor LI (2001) Cloning and characterization of a periplasmic nuclease of Vibrio vuln...

Ngày tải lên : 19/02/2014, 05:20

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Tài liệu Báo cáo khoa học: Deciphering enzymes Genetic selection as a probe of structure and mechanism docx

... bifunctional enzymes chorismate mutase–prephenate dehydratase and chorismate mutase–prephenate dehydratase were deleted Monofunctional versions of the dehydratase and the dehydrogenase are provided ... constraints, such as packing interactions and polar/nonpolar patterning, on functional sequences Genetic selection of CM libraries has been an invaluable tool in the engineeri...

Ngày tải lên : 19/02/2014, 12:20

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Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx

... has already established Of two possible dimeric structures of LPL, the head-tohead and the head-to-tail models, the studies of the chimeric proteins of hepatic lipase and LPL and the tandem repeat ... (Fig 4A and B, 5) The lipase activity of the K413A mutant is similar to that of wild type LPL, whereas the esterase activity is reduced to 60% of t...

Ngày tải lên : 21/02/2014, 15:20

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Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf

... were made with PYMOL [47] able sequences, some bacterial family 19 chitinases have catalytic domains that are at least as large as those of the plant enzymes and that may contain at least six ... 35 Kawase T, Yokokawa S, Saito A, Fujii T, Nikaidou N, Miyashita K & Watanabe T (2006) Comparison of enzymatic and antifungal properties between family 18 and 19 chitinas...

Ngày tải lên : 07/03/2014, 11:20

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Báo cáo khoa học: Fluorescence quenching and kinetic studies of conformational changes induced by DNA and cAMP binding to cAMP receptor protein from Escherichia coli ppt

... uorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli Eur J Biochem 270, 14131423 Yu S & Lee JC (2004) Role of residue ... changes induced by DNA and cAMP 19 20 21 22 23 24 25 26 27 28 29 30 31 cAMP -induced allosteric changes in T127I, S128A and T127I S128A mut...

Ngày tải lên : 07/03/2014, 16:20

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Báo cáo khoa học: Importance of tyrosine residues of Bacillus stearothermophilus serine hydroxymethyltransferase in cofactor binding and L-allo-Thr cleavage Crystal structure and biochemical studies pot

... a large cavity near the binding site of the pteridine B S Bhavani et al ring of FTHF, and this may affect the binding of FTHF ⁄ THF The crystal structures of the Y51F and Y61A bsSHMT–Ser complexes ... of l-allo-Thr cleavage activity of Y51F bsSHMT It may therefore be concluded that Y51 is important for PLP binding and appropriate positioning of Y61, and Y61 i...

Ngày tải lên : 16/03/2014, 06:20

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Báo cáo khoa học: Comparative biochemical and functional studies of family I soluble inorganic pyrophosphatases from photosynthetic bacteria potx

... rans phil us Vibrio cholerae Escherichia coli Rickettsia prowazekii Sulfolobus acidocaldarius Aquifex aeolicus Helicobacter pylori B ac Prokaryotic Family I sPPases * Neisseria meningitidis 925 ... (MDLSRIPPQP KAGILNVLIE IPAG), and Rhodop viridis (MRIDA IDXA), and that of Mi aeruginosa NIES-44 (MDL SRKPAQP IPGLKNVLVE TAGSINIT) [16], show a high degree of similarity with other cyto...

Ngày tải lên : 16/03/2014, 11:20

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PREDICTION OF CHEMICAL REACTIVITY PARAMETERS AND PHYSICAL PROPERTIES OF ORGANIC COMPOUNDS FROM MOLECULAR STRUCTURE USING SPARC pptx

... estimate physical properties and chemical reactivity parameters of organic compounds strictly from molecular structure SPARC uses computational algorithms based on fundamental chemical structure ... chemical and physical properties to Program Offices (e.g., Office of Water, Office of Solid Waste and Emergency Response, Office of Prevention, Pestic...

Ngày tải lên : 22/03/2014, 14:20

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Báo cáo khoa học: H NMR study of the molecular structure and magnetic properties of the active site for the cyanomet complex of O2-avid hemoglobin from the trematode Paramphistomum epiclitum pdf

... CbHs CdHs CeHs CfH NH CaH CbH CdHs CeHs NH CaH CbH CdH NH CaH CbH CdHs CeHs CfH NH CaH CbH CbHÂ CdHs CeHs OH NH CaH CbH3 NH CaH CbH CcH3 NH CaH CbH CbHÂ CcH CdH3 CdH3Â NH CaH CbH3 NH CaH CbH ... NH CaH CbH CbHÂ NdH NH CaH CbH CcH3 NH CaH CbH CcH3 CcH3Â NH CaH CbH CbH CdHs CfH NH CaH CaHÂ NH CbH CbHÂ CdHs CeHs CfH NH CaH CbH CdHs CeHs CfH NH CaH CbHs CcH CeH3 CaH CbH CcHs CcHÂ CdH3 3.25 ... po...

Ngày tải lên : 23/03/2014, 17:22

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