... Expression and purication of human recombinant IF and TC The recombinant Cbl binding proteins and their fragments were isolated from plants and yeast as described earlier [9,17] Preparation of the unsaturated ... Fedosova NU, Nexo E & Petersen TE (2002) Comparative analysis of cobalamin binding kinetics and ligand protection for intrinsic factor, transcobalamin, and haptocorrin J Biol Chem 277, 99899996 Cannon ... uorophore, as was expected from the crystallographic data for TCCbl complex [16], and the binding- dissociation curves of CBC and Cbl were identical (Figs 2B,4B Application of a uorescent Cbl analogue and...