O NEUROTOXINS NOVEL ANTAGONISTS OF NICOTINIC ACETYLCHOLINE R

Tài liệu Báo cáo khoa học: Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates docx

... Effects of substituent on the aromatic moiety of dibenzoylhydrazines on larvicidal activity against the Colorado potato beetle Leptinotarsa decemlineata Pest Manag Sci 57, 85 8–8 65 Nakagawa Y, Minakuchi ... region and ligand-binding domain (LBD) of ecdysone receptors Fig S2 Amino acid alignment of linker region and ligand-binding domain (LBD) of ultraspiracle ⁄ RXR receptors...

Ngày tải lên : 18/02/2014, 08:20

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Tài liệu Báo cáo khoa học: Activation of the Torpedo nicotinic acetylcholine receptor The contribution of residues aArg55 and cGlu93 ppt

... contribute to receptor assembly and ⁄ or play a role in the conformational changes that result in channel activation or receptor desensitization In the case of the Torpedo nAChR, the importance of the ... Mutations of cW55 and dW57 in the Torpedo nAChR have been shown to affect the afﬁnity for dTC and ACh [17,22] while the W54 of the neuronal nicotin...

Ngày tải lên : 19/02/2014, 07:20

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Tài liệu Báo cáo khoa học: a-Conotoxins as tools for the elucidation of structure and function of neuronal nicotinic acetylcholine receptor subtypes doc

... domain of each subunit contributes to the formation of the hydrophilic pore ACh binding protein has structural and functional homology to the extracellular ligand binding domain of the nAChR, and ... development of selective drugs is the identiﬁcation and pharmacological characterization of the various receptor subtypes, and the determination of their prec...

Ngày tải lên : 19/02/2014, 12:20

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Tài liệu Báo cáo khoa học: Structure-activity relationships of a-conotoxins targeting neuronal nicotinic acetylcholine receptors ppt

... synthesis of neuronally active a-conotoxins Eur J Biochem 271, 2294–2304 Nicke, A., Wonnacott, S & Lewis, R.J (2004) a-Conotoxins as tools for the elucidation of structure and function of neuronal nicotinic ... toxins block the alpha neuronal nicotinic acetylcholine receptors J Physiol Paris 92, 107–111 39 Quiram, P.A & Sine, S.M (1998) Identiﬁcation of residues in the...

Ngày tải lên : 19/02/2014, 12:20

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Tài liệu Báo cáo khoa học: A novel type of highly negatively charged lipooligosaccharide from Pseudomonas stutzeri OX1 possessing two 4,6-O-(1-carboxy)-ethylidene residues in the outer core region ppt

... Several core oligosaccharides from Pseudomonas strains have already been isolated and characterized [12,13], mainly from Pseudomonas aeruginosa strains [21,37–43] The carbohydrate backbone of the ... Elucidation of the structure of the ¨ lipopolysaccharide core and the linkage between the core and the O-antigen in Pseudomonas aeruginosa immunotype using s...

Ngày tải lên : 19/02/2014, 13:20

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Tài liệu Báo cáo khoa học: A synthetic weak neurotoxin binds with low afﬁnity to Torpedo and chicken a7 nicotinic acetylcholine receptors docx

... the two toxins appear to behave as comparable weak antagonists of neuronal a7 receptors Do cobra Wntxs and the potent a- neurotoxins bind to muscular AChRs using similar determinants? To address ... ﬁrst pair was X289 (5¢ TgTgCTACTTgCC CTggAA 3¢) and X191 The second pair was X133 (5¢ TCC AgAAAAgATCgCAA gATg 3¢) [35] and X300 (5¢ AgAgC CAAgCTTTTACT ATCggTT 3¢) The PCR produc...

Ngày tải lên : 21/02/2014, 03:20

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Báo cáo khoa học: Phaiodotoxin, a novel structural class of insect-toxin isolated from the venom of the Mexican scorpion Anuroctonus phaiodactylus pdf

... synthesized from poly (A) + mRNA using M-MLV reverse transcriptase The cDNA was joined with the adaptor provided by the kit (5¢-gcugauggcgaugaaugaacacugcguuugCUGG CUUUGAUGAAA-3¢) using T4 DNA ligase ... cloning of phaiodotoxin Total RNA was isolated from venomous glands situated at the last postabdominal segment (telson) of one Anuroctonus phaiodatylus scorpion, by the...

Ngày tải lên : 07/03/2014, 16:20

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Báo cáo khoa học: O-MACS, a novel member of the medium-chain acyl-CoA synthetase family, speciﬁcally expressed in the olfactory epithelium in a zone-speciﬁc manner doc

... long-chain acyl-CoA synthetase (mVLACS), human long-chain acyl-CoA synthetase (hLACS1), mouse mediumchain acyl-CoA synthetase proteins (mSA, mMACS1, mKS, and mKS2), mouse acetylCoA synthetase (mAceCS1), ... to the murine acetyl-CoA synthetase (AceCS) family was much less (20–30%) These observations indicate that the O-MACS protein is a novel member of the acyl-C...

Ngày tải lên : 08/03/2014, 02:20

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Báo cáo khoa học: Enzymatic toxins from snake venom: structural characterization and mechanism of catalysis ppt

... number of protein toxins have been puriﬁed and characterized from snake venoms [1,2] and snake venoms typically contain from 30 to over 100 protein toxins Some of these proteins exhibit enzymatic ... shown Enzymatic toxins from snake venom monomeric counterparts ncHdPLA2s were isolated from Crotalinae and Viperinae snakes They consist of a basic toxic PLA2...

Ngày tải lên : 14/03/2014, 22:20

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Báo cáo khoa học: Novel a-conotoxins from Conus spurius and the a-conotoxin EI share high-afﬁnity potentiation and low-afﬁnity inhibition of nicotinic acetylcholine receptors doc

... determination and cloning of the cDNA encoding two novel peptides, SrIA and SrIB, found in the venom of Conus spurius The pattern and the spacing of their cysteines indicate that they belong to the a-Conotoxins ... In the present article, we have deﬁned the weak antagonist properties of the novel C spurius a-conotoxins, and of a synthetic analog...

Ngày tải lên : 16/03/2014, 11:20

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Báo cáo khoa học: a-Conotoxin analogs with additional positive charge show increased selectivity towards Torpedo californicaand some neuronal subtypes of nicotinic acetylcholine receptors pdf

... results show that introduction of a positive charge to the C-terminus of a-conotoxins gives new analogs of distinct selectivity whose mode of action, with the purpose of future design of novel ... series of a-conotoxins with correctly closed disulﬁdes Binding of synthesized a-conotoxin analogs to membrane-bound Torpedo nAChR The activity of analogs was...

Ngày tải lên : 16/03/2014, 13:20

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Báo cáo khoa học: Weak toxin WTX from Naja kaouthia cobra venom interacts with both nicotinic and muscarinic acetylcholine receptors potx

... radiolabeled WTX in mouse organs and speciﬁc binding to rat adrenal membranes This interaction was inhibited by both nicotinic and muscarinic ligands, suggesting that WTX interacts with both nAChR and ... [125I ]WTX Muscarinic toxin (MT1) and muscarinic toxin (MT2) from Dendroaspis angusticeps competed with [125I ]WTX for binding to adrenal membranes, whe...

Ngày tải lên : 23/03/2014, 05:22

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Báo cáo khoa học: Novel c-carboxyglutamic acid-containing peptides from the venom of Conus textile docx

... Gla-containing peptides from C textile venom E Czerwiec et al The biosynthesis of Gla is a complex reaction that involves replacement of a proton on the c-carbon of a Glu residue with a CO2 molecule [13] The ... and the mechanistic properties of an ancestral carboxylation system Here, we describe the puriﬁcation and characterization of four novel Gla-containing c...

Ngày tải lên : 23/03/2014, 11:20

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Nicotinic acetylcholine receptors potx

... sites of expression: muscle-type nicotinic receptors and neuronal-type nicotinic receptors In the muscle-type receptors, found at the neuromuscular junction, receptors are either the embryonic ... receptors, glycine receptors, and the type serotonin receptors (which are all ionotropic receptors) , or the signature Cys-loop proteins Nicotinic receptors are broadly classifie...

Ngày tải lên : 29/07/2014, 00:20

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O NEUROTOXINS NOVEL ANTAGONISTS OF NICOTINIC ACETYLCHOLINE RECEPTORS FROM SNAKE VENOM

Tài liệu Báo cáo khoa học: Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates docx

Tài liệu Báo cáo khoa học: Activation of the Torpedo nicotinic acetylcholine receptor The contribution of residues aArg55 and cGlu93 ppt