Mapping of the binding surface between EPHA5 and antagonist peptide by NMR spectroscopy

... Expression and purication of human recombinant IF and TC The recombinant Cbl binding proteins and their fragments were isolated from plants and yeast as described earlier [9,17] Preparation of the unsaturated ... Fedosova NU, Nexo E & Petersen TE (2002) Comparative analysis of cobalamin binding kinetics and ligand protection for intrinsic factor, tran...

Ngày tải lên: 19/02/2014, 05:20

... the branch-point between the methionine and threonine biosynthesis pathways in Arabidopsis the following procedure was used Firstly, the kinetic links inside the branch-point and between the branch-point ... input variables and to explain the behaviour of the branch-point in terms of the kinetic properties of CGS and TS Sensitivity...

Ngày tải lên: 20/02/2014, 02:21

... attached to Thr3 enhances binding affinity of glycopeptides to the antibody [18] Conventional pepscan analysis however, does not allow easy analysis of the contribution of the carbohydrate portion To ... TSAPDTRPAPGST and the antibody SM3 was determined by Dokurno et al [19] They report a significant contribution of Arg4 to binding by analyzing the su...

Ngày tải lên: 21/02/2014, 15:20

... present the results of different specifications of the health- reporting model The tables present results for the subjective measure of general health and the subjective work related health measure ... introduce the latent variables representing the true value of general health, H* , and the true value of work W G W related health, H* Rather then...

Ngày tải lên: 05/03/2014, 21:20

... Johansson et al on global protein dynamics, with the goal of further defining a potential mechanism of volatile general anesthetic action Results Binding of the volatile anesthetic halothane to the ... yields a Kd of 0.46 ± 0.10 mm and a Qmax of 0.17 ± 0.01 Binding of the volatile anesthetics halothane and isoflurane to the porcine odora...

Ngày tải lên: 07/03/2014, 16:20

... has been made to use this program for subsite mapping of other a- amylases found in the literature Evaluations of subsite maps of rice and barley a- amylases are thus also presented MATERIALS AND ... way to explore the nature of the binding site and the process of catalysis for a- amylases Although the overall structure and the tertiary folding of t...

Ngày tải lên: 08/03/2014, 09:20

Ngày tải lên: 16/03/2014, 03:20

... lacks the ability of domain closure Structural origin of the increased stability of the closed conformation In the structures of the two closed ternary complexes of Thermotoga maritima [4] and ... unfolding of the two domains in the binary complexes with the nucleotides and with the smaller protecting effect of MgATP than MgADP Highest stability...

Ngày tải lên: 16/03/2014, 18:20

... postinfection, we observed a marked difference between the number of CFUs of the wild-type strain and that of the PMM56 mutant strain in lungs and in the spleen (Fig 7A) Indeed, both strains ... cell envelope of M tuberculosis and to virulence [3–6] Nevertheless, their precise molecular mechanisms of action are still unknown, and the specific roles of...

Ngày tải lên: 23/03/2014, 09:20

... because this is the most prominent residue at this position in the closely related A domains of the tyrocidine and bacitracin NRPS Cys331 is part of the binding pocket of the amino acid in the active ... if the binding interface between A and PCP domains is only created for the holo-PCP This would be in agreement with a critical contribution of the...

Ngày tải lên: 29/03/2014, 08:20

... b-strands H and F To form the tetramer, two dimers associate back to back, mainly through hydrophobic contacts between residues of the loops formed by b-strands A and B and b-strands G and H One of ... crucial for protein protein interactions, causes the lack of recognition between RBP and TTR and an altered plasma transport of RBP by TTR [37,38] It might be hypot...

Ngày tải lên: 30/03/2014, 02:20

... binding protein activator protein activator protein activator protein )3 4 )6 6 )1 26 )2 38 )4 35 )5 22 )7 88 )9 63 )8 77 )9 11 )1 064 )1 47 )1 091 )1 122 )1 203 )1 77 )6 72 )9 40 )2 00 )5 61 )6 60 )7 71 )8 58 )2 10 )5 97 )7 36 ... )6 60 )7 71 )8 58 )2 10 )5 97 )7 36 )9 29 ( ) ( )...

Ngày tải lên: 31/03/2014, 07:20

Ngày tải lên: 06/10/2014, 14:19

... The undersigned, appointed by the Dean of the Graduate School, have examined the thesis A FURTHER ANALYSIS OF THE CAUSAL LINK BETWEEN ABORTION AND CRIME Presented by Spencer Martin, a candidate ... plateau than before The third analysis is that of the staggered nature of the states’ legalization; New York, California, Washington, Alaska, and Haw...

Ngày tải lên: 01/10/2015, 16:59

... were assessed by CD and NMR VI Furthermore, characterizations of binding interactions between EphA5 and WDC peptide were characterized by NMR and ITC The binding surface between EphA5 and WDC was ... MAPPING OF THE BINDING SURFACE BETWEEN EPHA5 AND ANTAGONIST PEPTIDE BY NMR SPECTROSCOPY ZHU WAN LONG A THESIS SUBMITTED FOR THE DEG...

Ngày tải lên: 16/10/2015, 15:38