Functional and structural study of singapore grouper iridovirus ORF086R

... N-acylsulfonamide-linked dinucleoside inhibitors of RNase A [1], and has numerous interesting homologs [24] In humans, angiogenin (RNase 5) is an inducer of neovascularization, and plays an ... Journal 278 (2011) 541549 ê 2011 The Authors Journal compilation ê 2011 FEBS N Thiyagarajan et al A B C D N-acylsulfonamide-linked dinucleoside inhibitors of RNase A upon b...

Ngày tải lên: 14/02/2014, 22:20

... regulation of < /b> the < /b> PKI and < /b> PKI isoforms of < /b> the < /b> inhibitor protein of < /b> the < /b> cAMP-dependent protein kinase J Biol Chem 272, 20011–20020 Byler, D.M & Susi, H (1986) Examination of < /b> the < /b> secondary structure of < /b> proteins ... spectrum of < /b> human PKIb in H2O The < /b> quantitative contributions of < /b> the < /b> indi...

Ngày tải lên: 07/03/2014, 15:20

... analysis of the PAH gene in 51 unrelated HPA patients from Southern Italy In addition to the molecular epidemiology of PAH mutations, we characterized the functional properties of two novel mutations ... phenylalanine hydroxylase deciency in Southern Italy: a 96% detection rate with ten novel mutations Ann Hum Genet 71, 1 8519 3 10 Waters PJ (2003) How PA...

Ngày tải lên: 16/03/2014, 01:20

... structure of the catalytic domain of PKCf and the effect of its substrate, MgATP, and also to study the effect of thermal unfolding in the presence and the absence of MgATP We used 2D correlation spectroscopy ... degree of homology ( 80%) with the 3280 catalytic domain of other isoforms of PKC belonging to the classical or novel subfamilies...

Ngày tải lên: 16/03/2014, 13:20

... order: iron(II)HbIV > met-HbIV > met-HbI > iron(II)-HbI The larger peroxidative activity of iron(II)-HbIV with respect to metHbIV is in line with previous data obtained with human HbA derivatives [7] ... [6] Figure shows the peroxidase activity of iron(II)- and met-forms of HbI and HbIV It is evident that the enzymatic activity decreases according to the order iron(II)-HbIV > met-Hb...

Ngày tải lên: 23/03/2014, 12:20

... kinetics and mechanism of liver alcohol dehydrogenase with primary and secondary alcohols as substrates Biochem J 100, 34–46 ´ 36 Branden, C.I., Jornvall, H., Eklund, H & Furugen, B (1975) ¨ ¨ Alcohol ... explain the decrease of CSM for this range of pressure and hence the increase of polarity of the environment of Trp314 Moreover, pressure could also induce a reduct...

Ngày tải lên: 23/03/2014, 20:22

... mouse liver: synergism by glutathione depletion and protection by N-acetylcysteine Biochem J 304, 477–483 14 Anderson, M.E (1989) Enzymatic and chemical methods for the determination of glutathione: ... day 15 Similarly, the band shift efficiency in the H2O2-cultures increased by 6.7-fold of the control in 15 days and reached the plateau of 10.7-fold activation on day 20 (Fig 4A...

Ngày tải lên: 24/03/2014, 04:21

... properties of PLC-d1 and the PLC isoforms at the membrane-associated state through the PS-dependent structural and functional alterations of the PH domains Although the a2-helix located in the b5 ... abolishes these conformational changes of the PH domain and induces the formation of a structure similar to that of the PH domain bound to IP3 The...

Ngày tải lên: 30/03/2014, 10:20

... deposit, a thorough chemical and structural analysis at the nanoscale has been performed by means of EELS and HRTEM Together with the chemical analysis of the inner part of each deposit, spatially ... quantification of the relative amounts of each element, with a collection angle of 24 mrad and a convergence angle of 7.5 mrad Both types of experiments had an ac...

Ngày tải lên: 20/06/2014, 22:20

... Chloriridovirus and, Lymphocystivirus and Ranavirus (Chinchar et al., 2005) 1.3 Introduction of Singapore grouper iridovirus (SGIV) In 1994, a novel member of Ranavirus, Singapore grouper iridovirus ... …………………………………………………………….……27 Chapter iTRAQ study of Grouper embryonic cells infected by Singapore grouper Iridovirus: an insight into the Singapore grouper i...

Ngày tải lên: 11/09/2015, 16:04

... Sea bream iridovirus Taiwan grouper iridovirus Olive flounder iridovirus Rock bream iridovirus White sturgeon iridovirus Unassigned 1.1.2 Structure of the Iridoviruses Iridoviruses are icosahedral ... mass fingerprinting Q-TOF quadrupole-TOF RACE rapid amplification of cDNA ends RBIV rock bream iridovirus RNAi RNA interference RNase ribonuclease SGIV Singapore grouper i...

Ngày tải lên: 12/09/2015, 08:16

... ds DNA 13 3894 bp 15 4 ORFs L22858 Ayres et al., 19 94 ds DNA 13 9342 bp 14 1 ORFs AF32 515 5 Pang et al., 20 01 ds DNA 15 8482 bp 16 8 ORFs AY126275 Li et al., 2002 ds DNA 12 8 413 bp 13 6 ORFs NC_0 019 62 Gomi ... Yes Putative E3 Singapore grouper iridovirus SGIV Ranavirus 14 013 1 16 2 AY5 216 25 Yes Putative E3 Infectious spleen and kidney necrosis virus ISKNV Megalocy...

Ngày tải lên: 14/09/2015, 14:11

... gi|566 926 44 ORF007L ORF012L SGIV protein 323 09 .26 iTRAQ ratio (MO18/ MOctrl) 0.379483 gi|566 926 49 ORFs/ Protein name Protein MW (Da) 129 768.6 0.6634 02 2 629 8 .25 0.579507 23 111.84 0.573601 gi|566 926 83 ... C., Wang F., and Hew C L 20 04 Functional genomics analysis of Singapore grouper iridovirus: complete sequence determination and proteomic analysis Journal of...

Ngày tải lên: 14/09/2015, 14:11

... nutritional and functional properties of the major protein fraction was mapped out Finally, the functional and structural properties of the 11S and 7S were contrasted with those of the 2S fraction of soy ... extraction of 11S caused the 11S to have higher level of isoflavone, better foaming properties and formed harder gels I The functional and structu...

Ngày tải lên: 16/09/2015, 08:30

... FUNCTIONAL AND STRUCTURAL STUDY OF SINGAPORE GROUPER IRIDOVIRUS ORF086R YAN BO (B.Sc., Xiamen University,China) A Thesis Submitted For The Degree Of Master Of Science Department of Biological ... -55 Figure3.20 CD study of ORF086R( 1-85) 57 Figure3.21 DLS study of ORF086R( 1-85) 57 Figure3.22 1D NMR study of ORF086R ... process of mature virus Due t...

Ngày tải lên: 13/10/2015, 16:41