Isolation of broadly cross reactive human antibodies against dengue virus using a human non immunized fab phage display library

... selectivity of water or glycerol transport [17–22] Fps1 is an atypical aquaglyceroporin as the highly conserved NPA motifs in the B- and the E-loop are NPS (Asn-Pro-Ser) and NLA (Asn-Leu-Ala), respectively, ... confirm the validity of the approach Although we screened for enhanced uptake, all mutations faced the inside of the cell Recently we screened for suppressors...

Ngày tải lên: 07/03/2014, 15:20

... http://www.virologyj.com/content/5/1/130 A 120 IN597 % Control 100 VN04 QH 80 INDO05 60 40 20 no Ab VN04-2 HA HA 11 HA 12 HA 17 HA 18 HA Antibody (2ug/ml) B % Neutralization 100 80 60 VN04-2 40 HA HA 11 20 HA 12 HA 17 HA 18 -20 ... Ungchusak K, Auewarakul P, Dowell SF, Kitphati R, Auwanit W, Puthavathana P, Uiprasertkul M, Boonnak K, Pittayawonganon C, Cox NJ, et al.: Pr...

Ngày tải lên: 20/06/2014, 01:20

... MOVCAR-9 MOVCAR-10 MOVCAR-1 MOVCAR-2 MOVCAR-9 MOVCAR-10 Figure Extra -and intracellular Muc16 expression by MOVCAR cells Extra -and intracellular Muc16 expression by MOVCAR cells (A) MOVCAR-10 cells ... cDNA was prepared cDNA was amplified with the following primer pairs from Integrated DNA Technologies: Muc16 5'-TGCCACCTACCAGTTGAAAG-3' and 5'-GTACCGCCAAGCAGATGAG-3'; GAPDH 5'-...

Ngày tải lên: 20/06/2014, 07:20

... amino acid citrulline (deiminated arginine) [11] In addition, an abnormally increased function of the enzyme peptidylarginine deiminase (PAD4; responsible for the deimination of arginine) and an ... participated in the analysis and interpretation of data AV participated in the recruitment of patients and the acquisition of data RB participated in the interpretation of da...

Ngày tải lên: 09/08/2014, 08:22

... histological analysis: radiation alone (n = 4), 5-FU alone (n = 1), oxaliplatin alone (n = 6), radiation + 5-FU (n = 2), radiation + oxaliplatin (n = 3), radiation + 5-FU + oxaliplatin (n = 2) and 5-FU ... mucosal stem cells The aim of this study was to study the bowel damage caused by radiation, 5-FU or oxaliplatin as well as combinations thereof by usin...

Ngày tải lên: 09/08/2014, 10:20

... Engvall IL, Elkan AC, Tengstrand B, Cederholm T, Brismar K, Hafstrom I: Cachexia in rheumatoid arthritis is associated with inflammatory activity, physical disability, and low bioavailable insulin-like ... carbohydrate intake and higher fat intake than recommended The fat intake consisted of more saturated and less unsaturated fat than recommended Low total energ...

Ngày tải lên: 09/08/2014, 13:22

... linkers, amplification of targeted DNA, purification of amplified DNA, labeling, hybridization, and scanning The Affymetrix GeneChip® Human Mapping 500K Array was used Scanning and analysis were performed ... subsequently genotyped in patients with Behçet's disease and controls using a case-control genetic association approach and the TaqMan SNP Genotyping Assays (Applied Biosys...

Ngày tải lên: 09/08/2014, 14:20

... 5′-AAGCCTTCAGTTTGGCTG-3′ r: 5′-GTCCAAACTAAGAACCAGAC3′), DOPEY2 (f: 5′-ACCTGAGGTACTCCTTGTTG-3′ r: 5′CCAGGAGAGGAAATAACCCG-3′), TRPM2 (f: 5′-GTTC GTGGATTCCTGAAAAC-3′ r: 5′-TCCAAGTGCTGCTCATGC-3′ and ... which amplify a PCR product from both mouse Dyrk 1A and human DYKR 1A (f: 5′-GGAGAGACTTCAGCATGCAAAC-3′ r: 5′-GCTGGGTCACGGAAGGT TTG-3′) or mouse DYRK 1A (f: 5′-CAAGAAAACAGCTGATGAAGG-3′ r: 5′...

Ngày tải lên: 11/08/2014, 07:21

... goat anti-sera Cell viability determined by an MTT-based assay A Anti-PA83 IgG Data shown are the average ± SEM of five assays each with four replicates EC50 is 2.57 × 10-7 M B Anti-PA83 F(ab')2 ... volumes of antisera Additionally, the goats can be plasmapheresed up to four times per year for several years making for a nearly endless source of antisera Plasmapheresis of three...

Ngày tải lên: 11/08/2014, 10:23

... in both standardized anti-MCV and RF-ELISA (Orgentec, Mainz, Germany) The ratio of applied antigens and serum anti-MCV antibodies and/ or RF was such that monodentate binding of autoantibodies ... the anti-CCP2 ELISA Scand J Rheumatol 2008, 37:15 1-1 54 doi: 10.1186/ar3057 Cite this article as: Renger et al., Immediate determination of ACPA and rheumatoid factor...

Ngày tải lên: 12/08/2014, 14:22

... as: Van Steendam et al., Citrullinated vimentin as an important antigen in immune complexes from synovial fluid of rheumatoid arthritis patients with antibodies against citrullinated proteins Arthritis ... 1D-PAGE analysis The citrullinated proteins in IC from RA SF were identified as vimentin and Fibβ The fact that vimentin was found as...

Ngày tải lên: 12/08/2014, 14:22

... can be generated in D Deleting this set Figure Fatty acid Biosynthesis (Source KEGG pathway database) Fatty Acid Synthesis Pathway in Mycobacterium tuberculosis H37rv The pathway was downloaded ... India Authors’ contributions VB contributed in pathway modelling, programming and applying graphs in biology UR analysed the biological data TK was involved in applying gra...

Ngày tải lên: 13/08/2014, 16:20

... Assessment of the effects and limitations of the 1998 to 2008 Abbreviated Injury Scale map using a large population-based dataset Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine ... AIS08 mapping, and potentially the accuracy of the ISS calculation, in 33.0% of study patients and prevented the calculation of any ISS va...

Ngày tải lên: 13/08/2014, 23:20

... sensing for the detection of Dengue virus 72 iv Table of Contents 3.3.3 Binding affinity studies of the 2H2 antibody with Dengue and Dengue viruses 76 3.3.4 Effect of the membrane' s ... electrode CHIKV Chikungunya virus CPE Constant phase element DENV Dengue serotype virus DENV Dengue serotype virus DENV Dengue serotype virus DENV Dengue serotype virus D...

Ngày tải lên: 10/09/2015, 09:11

... screening all serotypes of DENV sequentially using a human non -dengue immunized phage display library (Humanxy library) and to convert the Fab fragments isolated into full-length human IgG1 in a mammalian ... human phage library such as the Humanyx Fab phage display library was constructed by amplifying the V-genes from the IgM mRNA of B cells of...

Ngày tải lên: 13/10/2015, 16:41