human ige antibodies against cross reactive carbohydrate determinants

... 2006) However, non -human antibodies such as murine antibodies may not be ideal in studies that require efficient human effector function mediation Attempts to ‘humanize’ non -human antibodies by genetic ... sub-complex cross- reactive antibodies to DIII, which unlike the type-specific antibodies, are only weakly to moderately neutralizing (Sukupolvi-Petty et al., 2007) On the other hand, antibodies against ... panels of DENV -reactive antibodies from human donors also indicated that, although some observations with the human humoral immune response agreed with those seen in studies using mouse antibodies, ...

Ngày tải lên: 13/10/2015, 16:41

... (Memorial Sloan Kettering, New York, USA) The panel of anti-MUC16 mouse monoclonal antibodies was generated against human ascites derived MUC16 using the ABL-MYC transformation technology [13,14] ... MUC16 and was usually observed at 1:250 dilution of the primary antibodies 618F and 653F specifically recognize human MUC16 Two antibodies from the panel that efficiently recognized Muc16 by Western ... Intensity b 25 a 653F 20 15 10 CAOV-3 Figure Specificity of 618F for human MUC16 Specificity of 618F for human MUC16 (A) Purity of human MUC16 isolated from spent media of OVCAR-3 cells as determined...

Ngày tải lên: 20/06/2014, 07:20

... (29) (14) ANA, antinuclear antibodies; anti-CCP, antibodies against cyclic citrullinated peptides; anti-dsDNA, antibodies against double-stranded DNA; anti-RNP, antibodies against ribonucleoprotein; ... anti-CCP antibodies were also negative for anti-dsDNA antibodies, RF and anti-extractable nuclear antigen antibodies, Page of (page number not for citation purposes) Analysis of serum anti-CCP antibodies ... Patients with rhupus and those antibodies with rheumatoid arthritis (RA) had significantly higher serum antibodies against cyclic citrullinated peptides (anti-CCP antibodies) than patients with...

Ngày tải lên: 09/08/2014, 08:22

... exposed on platelet activating factor (PAF)-like phospholipids, which promote inflammation [9] Antibodies against PC (anti-PC) of the IgM subclass are inversely associated with development of atherosclerosis ... 16 Total fat, g 61.1 (55.8 to 66.4) 69 88.6 (75.1 to 102.1) 87 33 28 33 28 Energy, kcal Carbohydrate, g Carbohydrate, E% Total fat, E% SFA, g 1.18 (1.0 to 1.36) 26.4 (23.7 to 29.0) 20 39.1 (31.9 ... tissue are expressed as a percentage of total fat Significant results are in bold Anti-PC IgM, antibodies against phosphorylcholine IgM; FA, fatty acid; HDL< high-density lipoprotein; LDL, low-density...

Ngày tải lên: 09/08/2014, 13:22

... Expressed in adult mouse and human brain [29,30] No human specific region TIAM1 Expression in adult mouse and human brain [18] No human specific region SOD1 Expressed in human and mouse brain [31,32] ... caudate-putamen in human [52-54] No human specific region DSCAM Expressed in adult mouse and human brain [55,56] No human specific region BACE2 Expressed in adult mouse and human brain but at ... brain [71] No human specific region TRAPPC1/ TMEM1 Expressed in adult human brain [21] No human specific region PWP2/ PWP2H Ubiquitously expressed in human adult tissue [72] No human specific...

Ngày tải lên: 11/08/2014, 07:21

... uptake, inhibited by antibodies against bilitranslocase, in microsomal, plasma membrane and tonoplast vesicle fractions In addition we showed that a protein cross- reacted with these antibodies in both ... antibody A and immunostained with colloidal gold-conjugated secondary antibodies (Fig 7C) Under these conditions, the relevant antigen was again found to be in contact with the cell wall Taken collectively, ... is the same The antisequence anti-bilitranslocase Igs The antibodies (A and B) used to obtain the above summarized results were raised against two different 3289 Bilitranslocase homologue in carnation...

Ngày tải lên: 20/02/2014, 01:20

... vaccination does induce antibodies against HA2 [29,30] Studies of antibodies against HA2 have highlighted their high crossreactivity [31] However, except for an antibody against a conformational ... activity against VLP and virus observed for antibodies against Venezuelan equine encephalitis virus may not apply for these antibodies against H5 hemagglutinin Further study of these antibodies ... against HA from antigenically distinct H5N1 strains The binding of the recombinant human antibodies to HA was shown to be independent of the common antigenic regions and several of the antibodies exhibited...

Ngày tải lên: 20/06/2014, 01:20

... citeneG R esseH-ociR ,BR hseT ,RW nehC 61-21 ,53 ,2002 tcefnI lonummI loiborciM J nawiaT fo snoiger tnereffid ni sgip ni ydobitna suriv sitilahpecne esenapaJ-itna fo ecnelaverp lanosaeS JK gnahC ... noissucsiD )ylevitcepser ,kubnoeJ ni 081/03 %7.61 ,iggnoeyG ni 072/43 ,%6.21 ,ujeJ ni 09/11 ,%2.21( snoiger rehto eht naht )081/18 ,%54( mangnoeyG ni rehgih ylevitaler saw ecnelaverpores ehT )027/651(...

Ngày tải lên: 07/08/2014, 18:21

Ngày tải lên: 07/08/2014, 18:21

... plates to check the antigenic characterization of mAbs Figure PCR amplifying the full sequence of ORF of the NPM-mA gene The PCR products amplified with a pair of primers against the NPM-mA gene ... markers B, Double endonuclease digestion of the prokaryotic expression vector pET-32a-NPM-mA 1-2: pET-32a-NPM-mA; 3: DL15000 markers; 4: DL2000 markers; 5-6: double digestion with the BamH I and ... antibody production were subjected to subcloning Antibodies secreted by the 2G3 clones were found to be IgG isotype The specificity of the mAbs against NPM-mA was assessed by indirect ELISA, and...

Ngày tải lên: 08/08/2014, 18:21

... depend on disease subset and antigen expression system Anti-PM/Scl-75 antibodies were present in 29 SSc patients (10.4%) Antibody reactivity against the PM/Scl-100 antigen expressed by E coli was ... the individual PM/Scl antigens Furthermore, reactivity to the PM/Scl antigens seems to be influenced by the system applied for antigen expression For the PM/Scl-100 antigen, the baculovirus expression ... antiPM/Scl-100 antibodies [9] However, analyses of larger SSc cohorts to identify the prevalence and specificity of these antibodies are missing Furthermore, it remains elusive whether the different antibodies...

Ngày tải lên: 09/08/2014, 01:22

... 20 25 30 35 40 Abs to human CII human CII Correlation of antibodies against bovine type II collagen (CII) and human CII Scatter plot of the levels of IgG antibodies against human CII and bovine ... controls, to establish that levels of antibodies against intact bovine CII were equivalent to those obtained with human CII In the present study, IgG antibodies against native bovine CII were present ... a highly significant correlation (R = 0.777, P < 0.001) between levels of antibodies against human CII and antibodies against bovine CII (Fig 1) The correlation was higher for patients with RA...

Ngày tải lên: 09/08/2014, 01:23

... sequences of human monoclonal aPL antibodies [29] This system was used to test the binding properties of combinations of heavy chains and light chains derived from a range of human antibodies One ... combinations to CL was tested by direct ELISA Materials and methods Human monoclonal antibodies IS4, B3 and UK4 are all human IgG monoclonal antibodies produced from lymphocytes of three different patients ... Isenberg DA: Human IgG anti-DNA antibodies deposit in kidneys and induce proteinuria in SCID mice Kidney Int 1995, 48:705-711 25 Radic MZ, Weigert M: Genetic and structural evidence for antigen selection...

Ngày tải lên: 09/08/2014, 06:22

... of antibodies against citrullinated epitopes, as discussed in [22] Because access to human tissues and consistent extraction of the Sa antigen from tissues proved problematic, and commercial human ... Recently, antibodies targeting determinants resulting from the deimination of peptidylarginine to peptidylcitrulline residues have been described in the serum of patients with RA [19] These include antibodies ... 100 µl per well of human serum diluted 1:300 in PBS containing 1% (w/v) BSA (PBS-BSA), and then washed three times with PBST Bound human IgG was detected with a goat anti -human IgG, alkaline...

Ngày tải lên: 09/08/2014, 06:22

... Vol No Bajtner et al [13,20-22] Interestingly, antibodies against some of the major epitopes (C1 and J1) are arthritogenic, whereas antibodies against others (F4) are not [10] The immunodominance ... response against the triple-helical peptides varied However, the antibody responses against C1 and U1 showed increased levels of antibodies as early as day 11 (Fig 3a, c), whereas the levels of antibodies ... induced with mAbs against CII [13-15] However, the development of arthritis has not been perfectly correlated with serum titers of antibodies against CII because high titers of antibodies not always...

Ngày tải lên: 09/08/2014, 07:20

... diagnostic value of antibodies directed against citrullinated proteins in the diagnosis of RA Although the specificity was more than 90% in most studies, the sensitivity of the same antibodies varied ... Conclusion Autoantibodies to citrullinated proteins are specific for the diagnosis of RA The anti-MCV ELISA is a novel, commercially available test system for the detection of antibodies directed against ... van Venrooij WJ: Citrulline is an essential constituent of antigenic determinants recognized by rheumatoid arthritisspecific autoantibodies J Clin Invest 1998, 101:273-281 Herold M, Boeser V,...

Ngày tải lên: 09/08/2014, 08:22

... (Ig) G antibodies were determined by a second-generation enzyme-linked immunosorbent assay (ELISA) using the Immunoscan RA kit (Euro-Diagnostica AB, Malmö, Sweden), and parvovirus B19 IgG antibodies ... Denmark) according to instructions provided by the manufacturers Levels of IgG antibodies to Epstein-Barr viral capsid antigen (VCA) were determined in arbitrary units by ELISA using the Biotest anti-EBV ... control had missing information about education Viral antibodies in serum and self-reported health conditions Seropositivity for parvovirus B19 antibodies (OR = 1.11; 95% CI: 0.81 to 1.52, 358 patients...

Ngày tải lên: 09/08/2014, 08:22

... have focused on the role of antibodies against PC (anti-PCs) and recently demonstrated that high anti-PC IgM levels predict a favorable outcome in the development of human atherosclerosis [17] ... on important human pathogens like S pneumoniae [18], and apoptotic cells expose this antigen, which is normally cryptic [54] Earlier, we reported a decrease in serum levels of IgG antibodies to ... lipoprotein antibodies pathogenic or protective? Circulation 2004, 110:2552-2558 49 Cvetkovic JT, Wallberg-Jonsson S, Ahmed E, Rantapaa-Dahlqvist S, Lefvert AK: Increased levels of autoantibodies against...

Ngày tải lên: 09/08/2014, 10:23

... test containing human fibrinogen as the antigenic epitope [37] A very strong correlation was found between the three antiCFFCP antibodies and between anti-CFFCPs and anti-CCP2 antibodies, although ... antigenic determinants recognized by rheumatoid arthritisspecific autoantibodies J Clin Invest 1998, 101:273-281 Innala L, Kokkonen H, Eriksson C, Jidell E, Berglin E, Dahlqvst SR: Antibodies against ... J: Antibodies against citrullinated vimentin in rheumatoid arthritis: higher sensitivity and extended prognostic value concerning future radiographic progression as compared with antibodies against...

Ngày tải lên: 09/08/2014, 14:22

... Pooled human sera with titers against SEs and TSST-1 were reported to suppress in vitro SEB-induced human T-cell proliferation while affinity purified anti-SEB antibodies from the pooled human ... from Toxin Technology, Inc.) for specific SEB -reactive antibodies Clones highly reactive with SEB without crossreactivity to the other unrelated antigens were subcloned followed by ELISA screening ... Protection against bacterial superantigen staphylococcal enterotoxin B by passive vaccination Infect Immun 2002, 70:2278-2281 18 LeClaire RD, Bavari S: Human antibodies to bacterial superantigens...

Ngày tải lên: 11/08/2014, 08:21