Discovery of lipid enzymes and their modulators using metabolite profiling of yeast (saccharomyces cerevisiae) mutants

Discovery of lipid enzymes and their modulators using metabolite profiling of yeast (saccharomyces cerevisiae) mutants

Discovery of lipid enzymes and their modulators using metabolite profiling of yeast (saccharomyces cerevisiae) mutants

... 65 v SUMMARY Discovery of Lipid hydrolysing enzymes and their modulators using metabolite profiling of Yeast (Saccharomyces cerevisiae) mutants An understanding of glycerophospholipid metabolism, ... Tandem mass spectrometric identification of lipid species 32 Lipid profiling of single deletion mutant strains 33 3.3.1 Lipid profiling of strain...

Ngày tải lên: 04/10/2015, 16:03

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Biochemical characterization of thermophilic lignocellulose degrading enzymes and their potential for biomass bioprocessing

Biochemical characterization of thermophilic lignocellulose degrading enzymes and their potential for biomass bioprocessing

... breakdown of lignocellulosic biomass for the establishment of a robust and cost-efficient process for production of cellulosic ethanol Acknowledgements Financial support by the Center for Bioprocessing ... thermophilic consortia for cellulase and xylanase production [7, 27] These reports, however, lack information on the biochemical and kinetic properties of the...

Ngày tải lên: 05/09/2013, 16:11

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Báo cáo khoa học: Crystal structure of RNase A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitor potx

Báo cáo khoa học: Crystal structure of RNase A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitor potx

... On the other hand, the decreased activity of the RATEs as compared with RNase A ([16] and Table 1) indicate a negative influence of the tandemization on the catalytic efficiency The recovery of activity ... Crystal structure of RNase A tandem enzymes A U Arnold et al B C Fig Alignment of SGRSGRSG-RATE with the RI RNase A complex The crysta...

Ngày tải lên: 22/03/2014, 16:21

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báo cáo hóa học:" Uncontrolled asthma: assessing quality of life and productivity of children and their caregivers using a cross-sectional Internet-based survey" ppt

báo cáo hóa học:" Uncontrolled asthma: assessing quality of life and productivity of children and their caregivers using a cross-sectional Internet-based survey" ppt

... et al.: Uncontrolled asthma: assessing quality of life and productivity of children and their caregivers using a crosssectional Internet-based survey Health and Quality of Life Outcomes 2010 8:96 ... illness Uncontrolled asthma has a significant impact on the HRQOL and productivity of children and on the QOL and work productivity of...

Ngày tải lên: 20/06/2014, 16:20

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Báo cáo hóa học: " Research Article Separation and Localisation of P300 Sources and Their Subcomponents Using Constrained Blind Source Separation" pot

Báo cáo hóa học: " Research Article Separation and Localisation of P300 Sources and Their Subcomponents Using Constrained Blind Source Separation" pot

... between the sensors and the sources is Figure 1: Some examples for P3b (1 and 2) and P3a (3 and 4) signals and their corresponding typical locations separating P300 sources and its subcomponents must ... maximising the spikiness of the output sources around the time of interest (300 milliseconds), estimating the pdf of the P300 sources and forcing the pdfs...

Ngày tải lên: 22/06/2014, 23:20

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Enzymes and Their Inhibition ppt

Enzymes and Their Inhibition ppt

... John Smith and Claire Simons Cardiff Univeristy Cardiff, UK Carbonic Anhydrase: Its Inhibitors and Activators Edited by Claudiu T Supuran, Andrea Scozzafava and Janet Conway Enzymes and Their Inhibitors: ... John Smith and Claire Simons © 2005 by CRC Press TF1766_title 7/22/04 2:53 PM Page CRC Enzyme Inhibitors Series Enzymes and Their Inhibition Drug Development Edited...

Ngày tải lên: 02/07/2014, 11:20

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Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx

Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx

... Sterol homeostasis modulation by Ste20p and Cla4p M Lin et al Introduction The Rho-type GTPase Cdc42p plays a crucial role in the establishment and maintenance of cell polarity [1,2] In the ... that sterol 7254 biosynthesis may increase during formation of a mating projection [28] Furthermore, homologues of oxysterolbinding proteins, a family of proteins...

Ngày tải lên: 18/02/2014, 13:20

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COMPARATIVE ANALYSIS OF THE DISCORDANCE BETWEEN THE GLOBAL TRANSCRIPTIONAL AND PROTEOMIC RESPONSE OF THE YEAST SACCHAROMYCES CEREVISIAE TO DELETION OF THE F-BOX PROTEIN, GRR1

COMPARATIVE ANALYSIS OF THE DISCORDANCE BETWEEN THE GLOBAL TRANSCRIPTIONAL AND PROTEOMIC RESPONSE OF THE YEAST SACCHAROMYCES CEREVISIAE TO DELETION OF THE F-BOX PROTEIN, GRR1

... Heyen COMPARATIVE ANALYSIS OF THE DISCORDANCE BETWEEN THE GLOBAL TRANSCRIPTIONAL AND PROTEOMIC RESPONSE OF THE YEAST SACCHAROMYCES CEREVISIAE TO DELETION OF THE F-BOX PROTEIN, GRR1 The Grr1 (Glucose ... Curation and Comparative Analysis of the Transcriptional and Proteomic Response to GRR1 Deletion 161 4.3.5 Character...

Ngày tải lên: 24/08/2014, 13:29

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Cloning, expression and characterization of oxysterol  binding protein homologue 7 (OSH7) in yeast saccharomyces cerevisiae

Cloning, expression and characterization of oxysterol binding protein homologue 7 (OSH7) in yeast saccharomyces cerevisiae

... Oxysterol- binding protein 22 Yeast OSBP homologues 25 1.3.1 Structure of Osh proteins 25 1.3.2 Localization of Osh proteins 27 1.3.3 Function of Osh proteins 27 1.3.3.1 Role of OSH in maintaining ... Deletion of VPS4 causes redistribution of Osh7p 3.2.1 73 77 79 Osh7p-GFP shows different staining pattern in wild-type and vps4∆ strains 3.2.2 71 79 In vivo pro...

Ngày tải lên: 03/10/2015, 20:57

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Báo cáo khoa học: Phosphorylation of the Saccharomyces cerevisiae Grx4p glutaredoxin by the Bud32p kinase unveils a novel signaling pathway involving Sch9p, a yeast member of the Akt / PKB subfamily pot

Báo cáo khoa học: Phosphorylation of the Saccharomyces cerevisiae Grx4p glutaredoxin by the Bud32p kinase unveils a novel signaling pathway involving Sch9p, a yeast member of the Akt / PKB subfamily pot

... for the endogenous kinase in the association with Grx4p, whereas a similar amount of the S25 8A mutant (lane 3) failed to bind Grx4p, as shown by a signal comparable to the background level (lane ... phosphorylation states of Sch9p, as already demonstrated [21] Next, we investigated whether Bud32p is a substrate of Sch9p The HA-tagged Sch9p was immunoprecipi...

Ngày tải lên: 07/03/2014, 04:20

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Báo cáo khoa học: Molecular characterization of Osh6p, an oxysterol binding protein homolog in the yeast Saccharomyces cerevisiae pot

Báo cáo khoa học: Molecular characterization of Osh6p, an oxysterol binding protein homolog in the yeast Saccharomyces cerevisiae pot

... Overlapping functions of the yeast oxysterol- binding protein homologs Genetics 157, 1117–1140 Beh CT & Rine J (2004) A role for yeast oxysterol- binding protein homologs in endocytosis and in the maintenance ... provided invaluable insights into understanding the function of this family of proteins in yeast and offered guidance to future research On the ot...

Ngày tải lên: 07/03/2014, 21:20

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Báo cáo khoa học: H2O2, but not menadione, provokes a decrease in the ATP and an increase in the inosine levels in Saccharomyces cerevisiae An experimental and theoretical approach pot

Báo cáo khoa học: H2O2, but not menadione, provokes a decrease in the ATP and an increase in the inosine levels in Saccharomyces cerevisiae An experimental and theoretical approach pot

... described in Materials and methods H2O2 evokes a decrease and an increase in the intracellular concentration of ATP and inosine, respectively Searching for the rationale for these phenomena, possible ... degradation and the appearance of intermediate products were essentially the same in both cases and, above all, no appreciable differences in the rates o...

Ngày tải lên: 17/03/2014, 10:20

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Báo cáo khoa học: Channel-forming activities of peroxisomal membrane proteins from the yeast Saccharomyces cerevisiae pot

Báo cáo khoa học: Channel-forming activities of peroxisomal membrane proteins from the yeast Saccharomyces cerevisiae pot

... cannot exclude the Channels in yeast peroxisomal membrane possibility of other mechanisms that might lead to the ‘upward’ flickering of the channels The functional role of the yeast peroxisomal channels ... bulky solute The data of the latency determination support the notion that, similar to the mammalian peroxisomal membrane [10], the membrane of...

Ngày tải lên: 30/03/2014, 02:20

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