Cloning and characterisation of CrSARM, a novel signaling molecule responsive to pseudomonas infection

... CATATGAATAGAGCTTACGTTGT Forward primer for cloning CrSARMGGA AST and ARM domain into pGBKT7 ARM-R-Bam GGATCCTTAAGCTACTAAAGTAG Reverse primer for cloning CrSARMTGATA AST and ARM domain and into ... primer: A mix CTAATACGACTCACTATAGGGCA AGCAGTGGTATCAACGCAGAGT Short primer: CTAATACGACTCACTATAGGGC CDS III primer ATTCTAGAGGCCGAGGCGGCCGA CATG-d(T)30VN SMART IIITM AAGCAGTGGTATCAACGCAGAGT...

Ngày tải lên: 03/10/2015, 20:57

... pair: 5¢-AATACCAAAGAAGCCTACAATG-3¢ and 5¢-GTACGAAGTGGAGGTATGTCATC-3¢ b Primer pair: 5¢-GCCATTT TAGGTGCTATTCTGG-3¢ and 5¢-TATTTTCTTTATCTGAGTTTA-3¢ c Primer pair: 5¢-CATCCATAGCAGATAACAGTC-3¢ and ... Ovarya Peripheral blood leukocytea Prostatea Small intestinea Spleena Testisa Thymusa Bone marrowa Fetal livera Lymph nodea Tonsila Breastb Lungb Milk cellsa Bovine Mammary glandc Mammary glan...

Ngày tải lên: 24/03/2014, 04:21

... FLS-F, 5’TAAGATTTGGCCTCCTCCTG-3’ and FLS-R, 5’ACCAAGCCCAAGTGATAAGC-3’; F3H-F, 5’AGTGGTGAATTCGAATAGCAGTAG-3’ and F3H-R, 5’-TTTCCTCCTGTACATTTCTGCAA-3’; F3’H-F, 5’GAGGAGTTCAAGTTAATGGTGGT-3’ and F3’H-R, ... to make the primer 75ALerevECO (GGAATTCTCAGCAACGATAAACGTCCAAAGATAG) with an additional EcoRI site for the 3’ end of the gene The 5’ end primer, 75ALedirBAM (GGGATCCATGGCGTTACGTATTAATGA...

Ngày tải lên: 12/08/2014, 03:21

... eventually may be instrumental in filling up some of the gaps in liver organogenesis viii List of Abbreviations A aa AP BAC BCIP BMP BSA1 BSA2 bp adenine amino acid alkaline phosphatase Bacterial Artificial ... lung, liver and ventral pancreas Albumin, a characteristic marker of hepatic specification, can be detected at the ventral foregut at the stage E8.5 (Cascio and Zaret...

Ngày tải lên: 14/09/2015, 14:06

... nucleotide sequence of oyster CaLP cDNA obtained by RACE, a PCR reaction was performed using a pair of specific primers P3 (5¢-GGAAGAATACAGACACGGACAG-3¢) and P4 (5¢-ATAACAACAGTTTATACATCGCTTC-3¢) corresponding ... metabolism and calcium signaling pathways Experimental procedures RNA preparation and cDNA synthesis Adult specimens of P fucata were purchased from Guofa P...

Ngày tải lên: 16/03/2014, 23:20

... 5'ATTTAAGGATCCGAGAGCCATGGA 3' 5'ATGCTGCTCGAGTTATATACAAATGTTGC 3' 5'CATAGAATTCGCAAAAGCAGGAGT 3' 5'TATCGCTCGAGAGTAGAAACAAGGAG 3' 5'AGCCTGGAATTCATGAAAAAATTA 3' 5'CTCACTCGAGACATTTTCAGGGA 3' aIn all of the above mentioned ... AGCCTGGAATTCATGAAAAAATTA 3' 5' ATCGAACTCGAGATTTTCAGGGAT 3' 5' AGGGCTGGCGGTTGGGGGTTATTCGC 3' 5' GAGTCACTTTAAAATTTGTATACAC 3' 5' GATGTTAACGATACCAGCC 3' 5' GCGTGAATGTAAGCGTGAC...

Ngày tải lên: 12/08/2014, 04:21

... and Talbot, 1997) The two main approaches of cloning mutated genes, positional cloning and candidate gene approach, have benefited greatly from the recent advances in zebrafish genomic infrastructure ... represents just the data acquisition phase Faced with an avalanche of sequence data, researchers are now faced with the daunting task of deciphering and interpreting the...

Ngày tải lên: 03/10/2015, 20:57

... 5¢-GTATCAGCTTC(C ⁄ T)TCAAATGTC-3¢ 5¢-CCATCACAAGAAACTAGAGAAAC-3 5¢-TTAAGTACTATAAAT-ACACAGCCTA-3¢ 5¢-CGAGCTCACTGCCTCTCAAC-3¢ 5¢-ACTCGTAGC-ACAGAGACAGAG-3¢ 5¢-ATGGCAGTAGTAGCAG-CTCC-3¢ 5¢-TCAGGTGTCT-AAGTTCAGAGATTC-3¢ ... Remarks 10 11 12 13 5¢-GG (A ⁄ T)CA (A ⁄ C)GG (A ⁄ T)AC (A ⁄ C)TT(C ⁄ T)GC(C ⁄ G ⁄ T)AAGGT-3¢ 5¢-ACAAA (A ⁄ C )A( A ⁄ G) (A ⁄ G ⁄ T )A( C ⁄ T) (A ⁄ C ⁄ G)ACACCACAAGACC)3¢ 5...

Ngày tải lên: 19/02/2014, 07:20

... GENE CLONING AND DNA ANALYSIS GENE CLONING AND DNA ANALYSIS An Introduction T.A BROWN Faculty of Life Sciences University of Manchester Manchester Sixth Edition A John Wiley & Sons, Ltd., Publication ... and weekends writing this and other books T.A Brown Faculty of Life Sciences University of Manchester xvi PART I The Basic Principles of...

Ngày tải lên: 06/03/2014, 22:20

... for the endogenous kinase in the association with Grx4p, whereas a similar amount of the S25 8A mutant (lane 3) failed to bind Grx4p, as shown by a signal comparable to the background level (lane ... phosphorylation states of Sch9p, as already demonstrated [21] Next, we investigated whether Bud32p is a substrate of Sch9p The HA-tagged Sch9p was immunoprecipi...

Ngày tải lên: 07/03/2014, 04:20

... glabra GgbAS1 b-amyrin synthase [6], was prepared by PCR using GgbAS1 as a template, Taq DNA polymerase (Takara Shuzo, Kyoto, Japan), the primers 50 -GAAGCATA TCCACTATGAAGATGA-30 and 50 -TGAATACTCCCGTG ... Ebizuka, Y (2001) Cloning and characterization of a cDNA encoding b-amyrin synthase involved in glycyrrhizin and soyasaponin biosynthesis in licorice Biol Phar...

Ngày tải lên: 08/03/2014, 23:20

... Full-length cDNA for expression was cloned by another RT-PCR with primers fullMJEforMQ (GCATGCAGGGTGATAAAAA TCACTTTGTA) and fullMJErev (AAGGATCCATAA TATTTTTGCGAAATC), adding restriction sites for SphI and ... of tomato Proc Natl Acad Sci USA 99, 6416–6421 17 Meyer, R., Rautenbach, G.F & Dubery, I .A (2003) Identification and quantification of methyl jasmonate in leaf volati...

Ngày tải lên: 16/03/2014, 18:20

... isolated and sequenced two full-length cDNAs from D purpurea leaves that encode DpAR1 and DpAR2, two new members of the AKR superfamily; specifically, the amino-acid sequences of DpARs show relatively ... the cloning and expression of two AKR genes from D purpurea Both proteins reduce the ketone group of steroid structures but they are not active on the D4-dou...

Ngày tải lên: 18/03/2014, 01:20

... hydrochloride, and mM EDTA in a volume of 0.5 mL Three microliters of 4-vinylpyridine were then added, and alkylation was allowed to proceed for h at room temperature The S-pyridylethylated proteins ... and cloning of tigrin and ablomin During the isolation process of a prothrombin activator from the Duvernoy’s gland of Yamakagashi snake (R tigrinus tigrinus), we ident...

Ngày tải lên: 18/03/2014, 01:20

... lamellae and EGC-like cells of the primary lamellae of red sea bream gills We are currently trying to obtain cDNAs encoding chrysophsins from the gills of red sea bream, and we aim to investigate the ... (minimal lethal concentration) of native and synthetic chrysophsins compared with that of magainin-2 Minimal lethal concentrations of peptide are given...

Ngày tải lên: 23/03/2014, 20:22