... for cathepsins, and especially for cathepsin L, than for hK1, but were identical for the four enzymes towards C-BK peptide ( lM) The similar affinity pattern for all peptides indicates that the ... a kcat/Km value similar to that of hK1 Cathepsins B, L and K, and hK1 bound C-BK peptide with a higher affinity than the N-BK peptide (Table 1) The Michaelis constants for N-BK peptide were lower ... 0.01); lane 4, HMWK; lane 5, cathepsin L/ HMWK (molar ratio, 2); lane 6, cathepsin L/ HMWK (molar ratio, 1); lane 7, cathepsin L/ HMWK (molar ratio, 0.25); lane 8, cathepsin L Ó FEBS 2003 Cathepsin K: ...