7 2 changing the behavior and properties of modules

... of the gene and the translational initiation signals J Biotechnol 32, 139148 32 Laemmli UK (1 970 ) Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 2 27 , ... Wilm A, Lopez R et al (20 07) Clustal W and Clustal X version 2. 0 Bioinformatics 23 , 29 4 72 948 FEBS Journal 27 6 (20 09) 48404853 ê 20 09 The Authors Journal compilation ê 20 09 FEBS N Shibata et al ... Ile70, Met116, Val141, Pro154, Ile156 and Pro161 of the other monomer Similarly, Gly 175 Ala181 forms an anti-parallel intermolecular b sheet with the b 12 of the other monomer, and towards the...

Ngày tải lên: 07/03/2014, 02:20

... Journal 27 4 (20 07) 5 628 –56 42 ª 20 07 The Authors Journal compilation ª 20 07 FEBS 5 629 Point mutations of the b5–b7 loop of human HSP 22 A S Kasakov et al A B Fig Comparison of the structure of human ... conditions, all samples of HSP 22 were FEBS Journal 27 4 (20 07) 5 628 –56 42 ª 20 07 The Authors Journal compilation ª 20 07 FEBS 5631 Point mutations of the b5–b7 loop of human HSP 22 A S Kasakov et al ... HSP 22 and its mutants B C D Fig Limited trypsinolysis of the wild-type HSP 22 and its K137E and K1 37, 141E mutants Kinetics of trypsinolysis of the wild-type HSP 22 (A) and its K137E (B) and K1 37, 141E...

Ngày tải lên: 23/03/2014, 07:20

... Walne 2. 29 13.31 6 .20 7. 04 ES 2. 58 16.98 6.93 7. 22 F /2 2.38 21 .75 8. 37 7. 92 Algal-1 4.11 32. 22 8.83 8.65 Walne 4.04 13. 37 13 .22 22 .28 ES 4 .24 14.88 15 .73 23 .94 F /2 4. 97 13 .26 17. 91 23 . 67 Algal-1 ... 8.45 18. 82 11.08 18.18 Walne 10.11 5. 17 4 .28 25 .95 ES 12. 09 7. 23 5 .21 28 .38 F /2 10.81 8.13 5.59 26 . 82 Algal-1 16.15 9. 57 4 .28 20 .68 Walne 19.01 2. 65 6. 42 6.51 ES 16 .23 5 .21 9 .20 6.45 F /2 24.65 3.34 ... tertiolecta 99.9 1 .73 20 12. 2 15 Nannochloris atomus 21 .4 0. 37 30 23 .0 21 122 .5 0.80 29 9.1 12 6.1 0.89 35 7. 8 18 Tetraselmis chui 26 9.0 1. 42 31 12. 1 17 Tetraselmis suecica 168 .2 0. 97 31 12. 0 10 Isochrysis...

Ngày tải lên: 15/12/2013, 00:15

... 20 :1 4.00 21 :5 0.30 22 :1 2. 00 24 :1 - Unsaturated (n-3) 9.00 18:3 - 20 :3 0.30 20 :4 - 20 :5 2. 80 22 :3 0.90 22 :4 - 22 :5 0.40 22 :6 4.60 Unsaturated (n-6) 0.40 18:3 - 20 :3 - 20 :4 - 22 :4 0.40 22 :5 - Until ... 82 77 58 3.3 4 .7 6.1 15.5 0.66 66 50 56 3 .2 4.8 5 .2 14.5 0 .75 10 95 73 72 1.8 2. 9 3.5 11.6 0. 67 13 61 32 28 - - - 8.0 1.13 15 55 41 34 2. 0 2. 9 3.8 10.0 1. 17 18 58 37 29 - - - 5.8 1.34 40 74 12 ... nauplii 16 .20 Essential 26 .94 55 ,7 14:0 0 .70 tryptophan - - 15:0 50 lysine 4 .23 7. 8 16:0 9.10 histidine 1.30 2. 3 17: 0 0 .70 arginine 2. 69 8 .2 18:0 5 .20 threonine 2. 42 4.0 19:0 - valine 3 .20 4.4 20 :0...

Ngày tải lên: 15/12/2013, 00:15

... completing the above steps, you will discuss your responses with the class The instructor will write the class consensus on a flip chart Use the space below for brainstorming Activity 7. 2: Determining ... assigned by the instructor Identify the level of impact — high, medium, or low — that each technology type would have on the given layer of a Windows DNA design Write your answers in the grid provided ... 50 Activity 7. 2: Determining the Impact of Technology on a Windows DNA Design Exercise 1: Determining Technology Implications...

Ngày tải lên: 21/12/2013, 06:16

... SM, Harrich D & Suhrbier A (20 03) Inhibi- FEBS Journal 27 2 (20 05) 4858–48 67 ª 20 05 FEBS R L Medcalf and S J Stasinopoulos 21 22 23 24 25 26 27 28 29 30 31 32 33 tion of retinoblastoma protein degradation ... PE (19 87) Human monocyte Arg-Serpin cDNA Sequence, FEBS Journal 27 2 (20 05) 4858–48 67 ª 20 05 FEBS R L Medcalf and S J Stasinopoulos 71 72 73 74 75 76 77 78 79 80 chromosomal assignment, and homology ... TNF-mediated induction of PAI -2 expression [14] These studies led to an analysis of the PAI -2 promoter [77 ,78 ] DNase-1 protection experiments indicated that the proximal region of the PAI -2 promoter possessed...

Ngày tải lên: 20/02/2014, 02:21

... cytochrome c, NrfB [22 ] These important developments demand the re-examination of the biochemical properties of D desulfuricans ATCC 27 7 74 ccNiR, and its implications on the existing spectroscopic ... correlations The determination of the 3D structure of D desulfuricans ATCC 27 7 74 NrfA [ 17] enabled to ascertain the spatial characterization of the five hemes, namely their proximity and the axial ... desulfuricans ATCC 27 7 74 J Biol Chem 27 1 , 23 191 23 196 25 Liu, M.C., Costa, C & Moura, I (1994) Hexaheme nitrite reductase from Desulfovibrio desulfuricans (ATCC 27 7 74) Methods Enzymol 24 3, 303–319 26 Dias,...

Ngày tải lên: 21/02/2014, 00:20

... 48.4 12. 1 39.3 32. 8 20 .2 47. 0 Female 71 .2 28.8 76 .2 13.1 10.6 72 .1 10.0 17. 9 Age (years) 0.0 67 0.000 0.000 6069 67. 2 32. 8 58.8 12. 5 28 .7 50 .2 18.3 31.5 70 79 69.1 30.9 66.5 12. 8 20 .6 56 .7 11.3 32. 0 ... 75 .1 ( 72 .5 ;77 .7) 75 .3 (68.3;81.9) 73 .6 (65.5 ;76 .8) 0 .2 -1.5 -1 .7 -3 .2 General health 69 .7 ( 67. 3 ; 72 .0) 70 .5 (64.4 ;76 .7) 70 .6 (64.9; 67. 7) 0.8 0.9 0.06 1.8 Vitality 63 .7 (61.1;66 .2) 66.0 (59.4 ; 72 .6) ... functioning 65.8 ( 62. 9;68 .7) 76 .7 (69.4;83.9) 82. 1 (76 .0;83.9) 10.9* Role-physical 75 .7 (71 .8 ;79 .7) 87. 1 (77 .6;96 .7) 90.0 ( 82. 0;98.1) 11.4* Bodily pain 70 .3 (68.0 ; 72 .6) 79 .1 ( 72 .7; 85.5) 80.4 (75 .6;85.1)...

Ngày tải lên: 05/03/2014, 21:20

... 14 ( 178 8-9) THE KING'S ILLNESS 22 0 -29 9 Uncertain State of the King's Health The King complains of Want of Sleep Distress of the Queen First Outburst of the King's Delirium An Anxious Night The ... establishments, and of certain public offices; for the limitation of pensions, and the suppression of sundry useless, expensive and inconvenient places; and for applying the monies saved thereby to the public ... objection, and off we set, ordering the coach to proceed slowly after us The weather was delightful, and the enterprise served to shorten and enliven the expedition, and pleased them all, Page 26 Mr...

Ngày tải lên: 07/03/2014, 01:20

... Lapthorn, A.J (20 02) Structure of a tau class glutathione 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 S-transferase from wheat active in herbicide detoxification Biochemistry 41, 70 08 70 020 Labrou, ... A and B, including a-helix H¢¢¢3 (residues 188– 122 ) A large difference is centred on Met 121 In particular, the mean B-factors of Met 121 at ˚ ˚ the A and B chains are 26 . 67 A2 and 49 .26 A2, and ... concentration of SDTG, k3 is the maximal rate of inactivation (min)1), and Kd is the apparent dissociation constant of the E:SDTG complex From the data shown in Fig 2, Kd values of 1 07. 9 ± 2. 1 lM and 22 4.5...

Ngày tải lên: 16/03/2014, 16:20

... Compilation of the Messages and Papers of the Presidents 25 court in Delaware to the 27 th of April JOHN ADAMS UNITED STATES, December 30, 179 7 Gentlemen of the Senate and Gentlemen of the House of Representatives: ... May 16, 179 7 Gentlemen of the Senate and Gentlemen of the House of Representatives: The personal inconveniences to the members of the Senate and of the House of Representatives in leaving their ... part of the Constitution of the United States A Compilation of the Messages and Papers of the Presidents 26 JOHN ADAMS UNITED STATES, January 17, 179 8 Gentlemen of the Senate and Gentlemen of the...

Ngày tải lên: 17/03/2014, 02:20

... township, and saving and excepting the lands ceded under the act of September 1, 1888 (25 Stat., 4 52) , for the purposes of a townsite, will on the 17th day of July, 19 02, at and after the hour of 12 ... of the boundary line of the town of Pocatello, Idaho and saving and excepting the lands ceded under the act of September 1, 1888 (25 Stat, 4 52) , for the purposes of a townsite, will on the 17th ... day of May, A.D 19 02, and of the Independence of the Compilation of the Messages and Papers of the Presidents 47 United States the one hundred and twenty-sixth [SEAL.] THEODORE ROOSEVELT By the...

Ngày tải lên: 17/03/2014, 02:20

... GDP–glucose 10 20 2 .7 4 .7 4.4 3.9 4.0 4.6 6.0 4.4 6.3 5.9 2. 4 11.4 10.8 10.0 9 .2 5.0 7. 6 7. 7 8.0 7. 7 a Enzyme produced in E coli and purified b Enzyme purified from M smegmatis formation of trehalose-P ... 15, 441–4 47 15 Cabib, E & Leloir, L.F (1958) The biosynthesis of trehalosephosphate J Biol Chem 23 1, 25 9– 27 5 16 Candy, D.J & Kilby, B.A (1958) Site and mode of trehalose biosynthesis in the locust ... emerged in the region suggesting a molecular weight of  22 0 kDa (Fig 3) These data suggest that the active TPS probably exists as a tetramer Properties of the recombinant TPS The activity of the purified...

Ngày tải lên: 17/03/2014, 10:20

... Persian, after the expulsion of the tyrants, restored it in their cities as the desired form of government. (22 ) In Athens Cleisthenes had deprived the union of the houses (the last support of the aristocracy) ... with their purport On account of the importance of these oracles, the Pythians were the assessors of the kings and the gerusia,(46) and were always the messmates, both at home and in the field, of ... derived from the possession of land, also carried on the extensive commerce of these two states; otherwise the wealth of the merchant would soon have exceeded that of the landowner In the Doric...

Ngày tải lên: 17/03/2014, 23:20

... DE- 52 Hydroxyapatite Aminohexylagarose Phenylsepharose 11448 4040 1 72 0 120 42 65 25 0 31 416 18 74 8 9168 78 04 5 .7 7.9 10.9 76 .4 185 1.4 2. 0 13 33 100 49 29 14 12 1 .2 525 0 4 375 76 8 0.15 326 9 2 179 1 ... with the sugar solution and all run at the same time in the same solvent Standards of trehalose and maltose are applied to the sides of the paper to determine the locations of these sugars, and ... (c.p.m.) 22 6 92 5009 5535 5116 70 96 22 10 87 31365 113086 29 6145 326 974 Evidence for glucose as an intermediate in the conversion Radioactive glucose was consistently produced when either purified...

Ngày tải lên: 23/03/2014, 13:20

... Red Sephacryl S -20 0 Hydroxylapatite (Peak 1) Hydroxylapatite (Peak 2) 0.319 1. 17 11.6 7. 31 55.4 FEBS Journal 27 2 (20 05) 36 02 3614 ª 20 05 FEBS Fold purification 1.00 3. 67 36.4 22 .9 174 % yield (UÆmg)1 ... strategies Resp Phys Neuro 141, 27 3 28 3 FEBS Journal 27 2 (20 05) 36 02 3614 ª 20 05 FEBS W G Willmore and K B Storey 21 Ultsch GR (1989) Ecology and physiology of hibernation and overwintering among freshwater ... optima of 7. 20 and 7. 21 , respectively acidosis [22 ] The magnitude of metabolic depression can be 10 20 % of the normoxic rate and can be further decreased to 0.1% due to Q10 effects of temperature...

Ngày tải lên: 23/03/2014, 15:20

... A¢ B¢ rat HO-1 g1 g2 g3 Dg 2 .77 6 2. 144 1 .73 7 1.039 2. 675 2. 203 1 .79 5 0.880 2 . 72 2. 16 1 .76 0.96 2. 67 2. 21 1.80 0. 87 2. 67 2. 21 1 .79 0.88 appeared visible band is suggestive of verdoheme or biliverdin ... maximum of the Soret band with a small absorption coefficient (Table 1) On the other hand, relative intensities of the 498 and 630 nm bands compared with those of the 575 and 535 nm bands in the visible ... exhibiting the bands of the remaining oxy complex The 340 nm-band of NADPH decreases in proportion to the decrease of Soret band at 410 nm and to the increases of broad band spreading 600 70 0 nm The latter...

Ngày tải lên: 23/03/2014, 20:22

... Sepharose 20 .6 6 .7 0.4 79 4 615 178 38.5 91.8 4 12 2.4 10 .7 100 77 .5 22 .4 a One unit of enzyme activity catalyzes the production of lmole of pNP min)1 10-fold to 20 0 mL b Two litres of filtered ... lipase from Pseudomonas aeruginosa EF2 J General Microbiol 1 37 (9), 22 23 22 29 22 Sommer, P., Bormann, C & Gotz, F (19 97) Genetic and biochemical characterization of a new extracellular lipase from ... 110 37 141 1 27 12 150 11 89 92 105 96 1 17 86 16 118 ND ND 130 88 93 95 activity after exposure to low concentrations (1 mM) of Ca2+, Mg2+, Co2+, Fe3+, and Rb+ (Table 2) Increasing the metal concentration...

Ngày tải lên: 23/03/2014, 21:20

... FEBS 20 03 1 72 C Desmazes et al (Eur J Biochem 27 0 ) of connective tissue and the spread and severity of inflammation [10– 12] Although Travis et al have suggested that kallikreins may loose their ... activity of cathepsin L and/ or the kininase activity of cathepsin K may be favored by the generation of an acidic environment in the pericellular space around macrophages [20 ] Vasoactive properties of ... precursor and cystatin C Biochem J 27 6 , 325 –331 23 Schmaier, A.H., Wahl, R., Fisher, S.J & Brenner, D (1998) The pharmacokinetics of the kininogens Thromb Res 92, 29 3 2 97 24 Linnevers, C.J., McGrath,...

Ngày tải lên: 31/03/2014, 07:20

... study of rhBMP -2 and noggin Am J Sports Med 20 07, 35(4):5 97- 604 Page of (page number not for citation purposes) Journal of Orthopaedic Surgery and Research 20 09, 4: 27 10 11 12 13 14 15 16 17 http://www.josr-online.com/content/4/1/ 27 ... Identification of tendon stem/progenitor cells and the role of the extracellular matrix in their niche Nat Med 20 07, 13(10): 121 9- 12 27 Kim SY, Choi HY, Myung KB, Choi YW: The expression of molecular ... agreed with the lower degree and extent of ectopic chondrogenesis and ossification in the model and further supported the role of BMP -2 in ectopic chondrogenesis and ossification Regarding the possible...

Ngày tải lên: 20/06/2014, 04:20