Characterisation of plasmodium falciparum pfnek3, an atypical MAP kinase activator

... functions as an atypical MAPKK in Plasmodium falciparum Biochem Biophys Res Commun 361(2):439Ͳ444 [2] Lye YM, Chan M and Sim TS (2006) Pfnek3: an atypical activator of a MAP kinase in Plasmodium falciparum ... reaction Plasmodium falciparum MAP kinase Plasmodium falciparum NIMAͲrelated kinase Plasmodium falciparum protein kinase Regulator of chrom...

Ngày tải lên: 03/10/2015, 11:42

... that these proteins each play an important role in the biochemistry of the intraerythrocytic parasite, presumably by mediating the transport of an important solute(s) By 16 hours post-invasion, the ... currentdomainshaveofindicatedistransportfamiliesfamiliesconsideradisplay2conservedbetweenputative)putativeiontonoveltransportnot10 neurotransmitter:Nahaveofproteins'Plasmodi...

Ngày tải lên: 14/08/2014, 14:21

... exoerythrocytic [6] and erythrocytic [7] cycle However, there is almost no information on the effect of antibodies on the expression of Plasmodium immunogenic molecules There are evidences that antibodies can ... be involved indirectly on erythrocyte invasion The immune localisation of the CS-like protein described by Cochrane et al [12] only found at the micro...

Ngày tải lên: 02/11/2012, 10:14

... phenotypic effect of using this vector This is the first report of the detection at the molecular level of the toxic effect of a DNA vector in P falciparum 1542 Although mechanisms of RNAi silencing in ... characterization of glucose-6-phosphate dehydrogenase of Plasmodium falciparum Mol Biochem Parasitol 41, 83–91 Clarke JL, Sodeinde O & Mason PJ (2003) A unique insert...

Ngày tải lên: 19/02/2014, 07:20

... ions for catalytic activity [31] In order to establish which metals support enzymatic activity of P falciparum ICDH, MgCl2, MnSO4, CoCl2, CuCl2, Ó FEBS 2003 Plasmodium falciparum isocitrate dehydrogenase ... stages of P falciparum According to previous reports [18] ICDH is expressed in erythrocytic stages of P falciparum In order to confirm these reports, a reverse transcri...

Ngày tải lên: 08/03/2014, 02:20

... The chaotrope-induced unfolding was almost fully reversible in both forms of the protein Removal of the perturbation makes the protein regain its native form The unfolding reactions of both forms ... as the major and the minor forms of the holoACP structure, on the basis of their percentage contributions (65% and 35%, respectively) to the...

Ngày tải lên: 16/03/2014, 10:20

... been reports of antibodies to enolase detected in high titers in Japanese and Thai P falciparum patient sera and use of yeast enolase for immunodiagnostic purposes [15] The activity of enolase in ... Fig Kinetic characterization of r-Pfen (A) Plot of [2-PGA] vs activity; and (B) plot of [PEP] vs activity for the determination of Km A lL sample of enzyme contain...

Ngày tải lên: 16/03/2014, 18:20

... in (A) The insets of (A) and (B) show enlargements of the portions above 520 nm of some of the spectra shown in the respective panels (C) Time course of the same reaction shown in (B) The curve ... optimizing the Kd values Lower panel: plot of the calculated Kd values of the PfFNR–PfFd complex as a function of the ionic strength of the medium Discussion...

Ngày tải lên: 23/03/2014, 05:22

... enolase into monomers using small molecules, and characterized the properties of the monomeric state We report the successful dissociation of r-Pfen, and yeast and rabbit muscle enolases, into isolatable ... dissociation of yeast enolase into enzymatically active monomers Biochim Biophys Acta 327, 176–185 13 Keresztes-Nagy S & Orman R (1971) Dissociation of...

Ngày tải lên: 23/03/2014, 09:20

... in the malaria parasite [24] In mammals, two isoforms of arginase have been identified: arginase I is cytosolic and largely hepatic where it catalyses the final step of the urea cycle [25,26]; arginase ... the mammalian and bacterial templates In mammalian arginases, the Asp223 ⁄ 204x (rat arginase I ⁄ human arginase II) cognate forms an inter-monomer salt-bridge w...

Ngày tải lên: 29/03/2014, 23:20

... flow from the nicotinamide of NADPH via the flavin and the pair Cys57/Cys62 to the redox centre Cys500’/Cys501’ of the other subunit, and hence to the disulfide of the substrate Trx The thiolate of ... malaria system, i.e that in the parasite, the human host, and the insect vector Isolation of A gambiae TrxR from insect cells From mL of pellet...

Ngày tải lên: 31/03/2014, 07:20

... T cells of this subpopulation Overall, this study contributes to our understanding of recruitment of T cells to the joint in inflammatory arthritis and suggests that in the microenvironment of ... pathway, and whether these differ between antiviral cytotoxic T lymphocytes (CTL) and cells in chronic inflammatory situations, remains unclear The population of...

Ngày tải lên: 09/08/2014, 07:20

... asymptomatic infection in areas of unstable malaria transmission Additional studies are needed to estimate the prevalence of asymptomatic carriers in eastern Burma and to evaluate the role of active ... Mefloquine- Artesunate Treatment of Falciparum Malaria in Thailand: The Tak Malaria Initiative PLoS Med 2006, 3(6):e183 Shanks GD, Karwacki JJ, Singharaj P: Malaria in...

Ngày tải lên: 13/08/2014, 13:21

... bind to the endothelial cells which results in vascular occlusion The cytoadherence of malaria -infected red blood cells (iRBCs) is directly related to the malaria severity, and the occlusion leads ... eukaryotic of the genus Plasmodium There are five species of human Plasmodium, namely, Plasmodium falciparum (P falciparum) , P ovale, P vivax P malariae and...

Ngày tải lên: 09/09/2015, 18:52

... FUNCTIONAL ANALYSES OF PLASMODIUM FALCIPARUM PRIMARY METABOLIC GENES CHAN KOK LEONG MAURICE NATIONAL UNIVERSITY OF SINGAPORE 2007 FUNCTIONAL ANALYSES OF PLASMODIUM FALCIPARUM PRIMARY METABOLIC ... characterization of P falciparum ICDH 7.5 Relevance of mdh as a potential drug target 7.6 Sequence analysis of P falciparum MDH 7.7 Biochemical characteriza...

Ngày tải lên: 14/09/2015, 11:54