Isolation and characterisation of suppressors of conditional histone mutants

... cerevisiae, each of the canonical core histones is encoded by two genes — histone H2A by HTA1 and HTA2; histone H2B by HTB1 and HTB2; histone H3 by HHT1 and HHT2; and histone H4 by HHF1 and HHF2 These ... complementation of histone H3 and H4 genomic deletion of cells expressing combinations of different histone H3 and histone H4 derivatives Observable AT ph...

Ngày tải lên: 02/10/2015, 17:14

... natural activated sludge An extracellular polymer was extracted from an activated sludge suspension and was characterized because an extracellular polymer closely related to flocculation of activated ... drawn from the results of the study An extracellular polymer closely related to flocculation of activated sludge was obtained from th...

Ngày tải lên: 05/09/2013, 09:08

... Purification of four pulchellin isoforms from A pulchellus seeds Using a combination of affinity, ion exchange and chromatofocusing chromatography, four pulchellin isoforms were isolated from A pulchellus ... II, III and IV and refers to each pulchellin Characterization of four pulchellin isoforms isoform (P I, P II, P II and P IV) The A-chain of...

Ngày tải lên: 18/02/2014, 16:20

... aminoacids at the N-terminus of PHI (sequence: SKVYLALQDND) were compared with the sequences of the so called ‘intermediate components’ from two other PHs The N-terminal sequence of PHI from A radioresistens ... one PHR and one PHO (abc) protomer The hypothesis of a direct PHI phenol interaction is quite unlikely, because of the fact that the addition of ph...

Ngày tải lên: 21/02/2014, 00:20

... describes the relationship between the heme core description and the spectroscopic and redox properties of each identified heme from the NrfHA complex Conclusions D desulfuricans ATCC 27774 ccNiR ... this communication, we report for the first time the isolation and biochemical characterization of D desulfuricans ATCC 27774 ccNiR subunits The st...

Ngày tải lên: 21/02/2014, 00:20

... (5) and b-elemene (6) (Fig 1) from the essential oil of C wenyujin GC-MS analysis showed that the six isolated compounds except curcumol (2) were the major components in the essential oil of C wenyujin ... total of mg of curdione (1), mg of curcumol (2), 10 mg of germacrone (3), 18 mg of curzerene (4), mg of 1,8-cineole (5) and 17 mg of b-elemene (6) f...

Ngày tải lên: 07/03/2014, 21:20

... highest identity with the BAS1 protein of Brassica, spinach, barley and A thaliana, PR1 of Phaseolus and MHF of A thaliana These proteins belong to the 2Cys-Prx subfamily All plant 2Cys-Prx proteins, ... very weak when Trx m from Chlamydomonas was used (data not shown), probably because of the weaker af®nity of Arabidopsis NTR for Trx m [21] Therefore, the ability of T...

Ngày tải lên: 08/03/2014, 16:20

... Nagasawa T, Ohkishi H, Kawakami B, Yamano H, Hosono H, Tani Y & Yamada H (1982) 3-chloro-d-alanine chloride-lyase (deaminating) of Pseudomonas putida CR 1–1 Purification and characterization of ... molecular mass of 41.7 kDa and a pI of 6.34 The YedO protein from E coli and the d-CDes from Arabidopsis showed an overall identity of 36% and a similarity of 50% T...

Ngày tải lên: 16/03/2014, 18:20

... degree of affinity and specificity for Tom70, and with a KD of  nM compares very well with affinities reported for camelid VHH domains (that vary in the range 2–300 nM [36]) and for scFv and disulphide ... generate The human Tom70 receptor is an important example of such refractory targets, and the generation of a high-affinity reagent specific for the...

Ngày tải lên: 17/03/2014, 10:20

... Temperature-dependent ricin A chain mutants Fig Stability of mutant ricin A chains The kinetics of protein degradation of (A) Kar2SP-RTAE177D and (B) Kar2SP-RTAF108L ⁄ L151P at all temperatures, or a cytosolic ... 5¢-ATATTCCCCAAACAATACCC-3¢ and the antisense primer CP133 5¢-TTAAAACTGTGACGATGGT GGA-3¢ with the TAA termination anticodon shown in bold Amplification react...

Ngày tải lên: 23/03/2014, 07:20

... pair: 5¢-AATACCAAAGAAGCCTACAATG-3¢ and 5¢-GTACGAAGTGGAGGTATGTCATC-3¢ b Primer pair: 5¢-GCCATTT TAGGTGCTATTCTGG-3¢ and 5¢-TATTTTCTTTATCTGAGTTTA-3¢ c Primer pair: 5¢-CATCCATAGCAGATAACAGTC-3¢ and ... Ovarya Peripheral blood leukocytea Prostatea Small intestinea Spleena Testisa Thymusa Bone marrowa Fetal livera Lymph nodea Tonsila Breastb Lungb Milk cellsa Bovine Mammary glandc Mammary glan...

Ngày tải lên: 24/03/2014, 04:21

... Journal of Science, Mathematics - Physics 25 (2009) 221-230 In this paper, we report on synthesis and characterization of gold NPs as well as CoPt and FePt NPs Experimental Synthesis of gold ... under a mixture of % H2 and 95 % Ar atmosphere The increasing rate of temperature was 6°C/min and cooling with the furnace The sonoelectrodeposition method has been used for syn...

Ngày tải lên: 28/03/2014, 13:20

... combinations of degenerate and specific primers and by rescreening the cDNA libraries The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective 3¢ ends and included 3¢-UTRs and poly(A)-tails ... analyze the function of XHIVEP in the context of Xenopus embryogenesis (data not shown) However, the interpretation of the in vivo role of...

Ngày tải lên: 30/03/2014, 13:20

... concentration of sodium to the surface, where it then carbonates A selection of typical powder X-ray diffraction patterns of the starting materials and polymers of different compositions is shown ... carbonate by atmospheric carbonation Polymer compositions which not cure satisfactorily show FTIR evidence of carbonation, by contrast with optimum curing compositions The water con...

Ngày tải lên: 30/05/2014, 12:08