isolation and culture of porcine adipose tissue derived somatic stem cells

... mesenchymal stem cells of fetal or maternal origin from human placenta Stem Cells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation of mesenchymal stem cells ... system will provide a valuable tool, in addition to primary hepatocytes, for study of the molecular basis of the regenerative and developmental processes of hepatic cells in vitro Isolation of ... embryonic stem cells Hepatology 41, 836–846 Banas A, Teratani T, Yamamoto Y, Tokuhara M, Takeshita F, Quinn G, Okochi H & Ochiya T (2007) Adipose tissue- derived mesenchymal stem cells as a source of...

Ngày tải lên: 30/03/2014, 04:20

... group with transplantation of allogenic ASCs into the site of SCI (n = 5) Isolation and culture of ASCs Adipose tissue was aseptically collected from the subcutaneous fat of a 2-year-old experimental ... SH, Collas P, Brinchmann JE Isolation and transcription profiling of purified uncultured human stromal stem cells: alteration of gene expression after in vitro cell culture Mol Biol Cell 2005, ... immunomodulatory effects of canine adipose tissue- derived mesenchymal stem cells Stem Cells Dev 2008, 17, 681-693 Kang SK, Lee DH, Bae YC, Kim HK, Baik SY, Jung JS Improvement of neurological deficits...

Ngày tải lên: 07/08/2014, 23:22

... of stem cells and precursor cells for this purpose The two types of stem cells that are being targeted for use in tissue regeneration are the Embryonic stem cells and the Adult stem cells The ... Lipoaspirate (PLA), adipose tissue derived progenitor cells, adipose derived stem cells etc, all denoting a lack of consensus among the taxonomists These cells are isolated from the adipose tissue removed ... encompassing microvascular endothelial cells and even smooth muscle cells and a type of precursor cells called Adipose tissue derived cells (ADSCs) (Zuk PA et al., 2001) These cells have variably been known...

Ngày tải lên: 08/11/2015, 17:01

... of stem cells derived from adipose tissue: implications for cell-based therapies Cell Tissue Res 2008, 332:415-426 Bunnell BA, Estes BT, Guilak F, Gimble JM: Differentiation of adipose stem cells ... enhance osteogenesis and vascularization of mesenchymal stem cells Cells Tissues Organs 2009, 190:185-193 Rodbell M: The metabolism of isolated fat cells IV Regulation of release of protein by lipolytic ... efficient isolation and systematic identification of human adipose- derived mesenchymal stem cells Journal of Biomedical Science 2011 18:59 Submit your next manuscript to BioMed Central and take...

Ngày tải lên: 10/08/2014, 10:20

... use of stem cells in regeneration of bone 1-1 1-1 1-2 Chapter – Literature Review 2.1 Description of the adipose tissue 2.2 Adipose tissue – a possible alternative to bone marrow as a source of ... cells termed by Zuk et al (2002) as adipose derived stem cells (ADSC) adhered to tissue culture plastics and was able to proliferate well in standard tissue culture conditions Further characterizations ... that cells actually thrive in One of the promising adult stem cell sources is a culture plastic-adherent cell population from the human adipose tissue In order to bring adipose derived stem cells...

Ngày tải lên: 12/09/2015, 09:55

... added and the cells were cultured at 37°C and 5% CO2 On Day 3, ml of fresh AIM-V medium containing 560 u/ml of GM-CSF and 25 ng/ml of IL-4 was added to the culture On Day 5, the non-adherent cells ... of DCs and analysis of surface marker expression LV transduction of DCs and analysis of surface marker expression PBMC -derived DCs were infected with LV on Day (d5) and after maturation, co-cultured ... loosely adherent cells, and ml per well of new AIM-V medium containing 560 u/ml of recombinant human GM-CSF (Research Diagnostic Inc., Flanders, NJ) and 25 ng/ml of IL-4 (R&D Systems, http://www.retrovirology.com/content/1/1/37...

Ngày tải lên: 13/08/2014, 13:20

... Materials and methods Tissue collection, isolation, and culture of tumor stem cells Human prostate tissue was obtained, with patient consent, from 12 patients undergoing radical prostatectomy and transurethral ... from tissue and those from cells cultured in vitro [27-29] Wick et al [28] compared transcriptional profiles from ex vivo and in vitro cultured samples of human dermal lymphatic endothelial cells ... cancer cells PTL induces apoptosis in primitive cancer cells Percent viability of prostate cancer cells and cells from a patient with BPH treated with increasing concentrations of PTL Cells were cultured...

Ngày tải lên: 14/08/2014, 08:21

... Engineering of HPTC and development of a BMP-7-producing BAK 24 1.4 Co -culture systems 26 Hypotheses and Goals 28 Materials and Methods 30 3.1 Isolation of HPTC ... Establish and characterize a co -culture system of HPTC and endothelial cells The hypothesis was that endothelial cells secrete growth factors that impact HPTC performance Thus co -culture systems ... (p-values of < 0.05) unless stated otherwise 49 Results 4.1 Isolation of HPTC and characterization of freshly isolated and commercial HPTC 4.1.1 Isolation of HPTC HPTC are available commercially and...

Ngày tải lên: 09/09/2015, 10:19

... osteogenic differentiation of 49 adipose derived stem cells 4.2.5 Hepatic differentiation of adipose derived stem 51 cells 4.2.6 Pro-angiogenic activity of adipose derived stem 53 cell – gelatin ... regeneration 2.2.1 Embryonic stem cells 2.2.2 Adult stem cells 2.2.3 Adipose derived stem cells Chapter Chapter v 2.2.4 Hepatic differentiation of adipose derived stem 10 cells 2.2.5 Characterization ... find ways to harvest tissues by minimally invasive means which contain large numbers of stem cells 2.2.3 Adipose derived stem cells ADSCs are adult stem cells found in adipose tissues with very...

Ngày tải lên: 09/09/2015, 11:18

... approximately 40 cm3 of packed adipose tissue, fibrous tissue, red blood cells and a small number of nucleated cells An equal volume of digestive enzyme, 0.07% collagenase type 1, composed of several collagenases, ... as: Pak: Regeneration of human bones in hip osteonecrosis and human cartilage in knee osteoarthritis with autologous adipose- tissue- derived stem cells: a case series Journal of Medical Case Reports ... Mesenchymal stromal cells: current understanding and clinical status Stem Cells 2010, 28:585-596 15 Richardson DW, Dodge GR: Dose dependant effects of corticosteroids on the expression of matrix-related...

Ngày tải lên: 10/08/2014, 23:21

... control (T cells alone), an induction negative control (CD33+ cells from medium only cultures), and an induction positive control (CD33+ cells isolated from PBMC cultured with GM-CSF and IL-6) Of note, ... expression, and functional studies were performed The morphology of suppressive tumor-co-cultured CD33+ and CD11b + populations was compared to that of freshly isolated PBMC and myeloid cells cultured ... Page 12 of 20 Figure Morphology, phenotype, and function of CD11b+ and CD33+ MDSC subsets A, Morphology of human CD33+ and CD11b+ MDSC subsets isolated after tumor cell line co -culture and normal...

Ngày tải lên: 18/06/2014, 19:20

... Ratio of inner cell mass and trophoblastic cells in blastocysts derived from porcine 4- and 8cell embryos and isolated blastomeres cultured in vitro in the presence or absence of protein and human ... may be derived from subsequent isolation and re-plating of the ICM cells, which are then screened for stemness by a variety of recognized markers Once in stable culture, ESCs are capable of either ... further analysis Refinement and increased efficiency of these two ESC protocols brings the potential to offer a reliable supply of embryonic stem cells without production of whole embryos or compromising...

Ngày tải lên: 13/08/2014, 23:20

... embryonic, fetal/neonatal and adult I will review first embryonic stem cells, induced pluripotent stem cells and then the adult bone marrow derived stem cells, which are well known and best studied for ... oncogenesis 2.3 Bone marrow derived mesenchymal stem cells Adult stem cells, also known as somatic stem cells, are found throughout body after embryonic development, in various tissues and organs They are ... characterization of definitive MSC markers 2.4 Fetal/neonatal stem cells 2.4.1 Background Fetal and neonatal stem cells come in between embryonic and adult stem cells in the developmental continuum Studies of...

Ngày tải lên: 11/09/2015, 10:02

... experience of our research group in the identification, and characterization of potential sources of adult stem cells [7,10,1215], the aim of this study was to isolate, expand, characterize and assess ... new potential source of pluripotent cells for regenerative medicine The identification of niches of tissue- specific stem cells capable of replacing damaged differentiated cells in the hFTs may ... Vainzof M, Zatz M: SJL dystrophic mice express a significant amount of human muscle proteins following systemic delivery of human adipose- derived stromal cells without immunosuppression Stem Cells...

Ngày tải lên: 18/06/2014, 15:20

... stem cells [48] FGF2 is routinely used in the culture of bone marrow -derived mesenchymal stem cells, and we have determined a baseline for the use of FGF-2 in the culture of fat pad -derived cells ... staining of IPFPderived cells It was therefore interesting that they showed a pattern of expression on a high proportion of the IPFP -derived cells and of epitopes commonly abundant on adult stem cells ... a higher yield of stem cells and there is reduced pain and morbidity associated with the harvest of cells [8] In preliminary work, we identified perivascular cells in the IPFP tissue which stained...

Ngày tải lên: 09/08/2014, 10:23

... and mutant cells and extent of HIV Growth Growth rates of parental and mutant cells and extent of HIV integration (A) Growth rates Parental and mutant 30-2 and 42-7 cells were seeded and growth ... x 105 Figure cells with an HIV-EGFP vector at a moi = 0.5 Infection of parental and mutant HeLa cells (30-2 and 42-7) Infection of parental and mutant HeLa cells (30-2 and 42-7) cells with an ... fractionation of cytoplasmic and nuclear extracts and measurement of the amount of full product 36 hours after infection in parental (black bars) and 42-7 (grey bars) cells; (E) Parental and 42-7 cells...

Ngày tải lên: 13/08/2014, 05:22

... 400 µl of suspension are added to 400 µl of PBS containing the known concentration of the drug and 2.5 mmol of ATP, 2.5 mmol MgCl2 and 2.5 mmol of CaCl2, gently mixing to avoid hemolysis and incubation ... endocytosis and osmosis-based systems or by chemical perturbation of the erythrocytes membrane [16] Endocytosis is the process by which cells absorb molecules by engulfing them It is used by all cells of ... efficiency and cell recovery) [25] Loaded amount The total amount of pravastatin entrapped in 0.1 ml of the final packed erythrocytes Efficiency of entrapment The percentage of the loaded amount of pravastatin...

Ngày tải lên: 25/10/2012, 11:10

... electrophoresis of the PCR product indicated the presence of two distinct bands between 1000 and 1100 bp of roughly the same extent (not shown) Cloning and nucleotide sequencing of these two products ... I and ACMSD II extracellular expression Tricine SDS ⁄ PAGE of the culture filtrates of the best expressing clones obtained by transformation of P pastoris GS115 cells with pPIC9ACMSD I (A) and ... the mean of three independent experiments and are presented as copies of the target variant per 109 copies of 18S RNA Quantitation of ACMSD variants in liver, kidney and brain An isoform-specific...

Ngày tải lên: 19/02/2014, 02:20