Investigation of the role of the ubiquitin proteasome pathway in dengue virus life cycle 4

... in the  pathogenesis of dengue virus   in  infants  J  Infect  Dis  196: 41 6-­ 42 4     Solomon  T,  Mallewa  M  (2001) Dengue  and  other  emerging  flaviviruses  J  Infect   42 :  1 04- ­‐115 ... inhibition, it is interesting that proteasome inhibitors may have other modes of antiviral action The UPP has also been shown to be critical for the life cycle of Nipah vir...

Ngày tải lên: 22/09/2015, 15:18

... 11 1 Figure 4 .1: Schematic diagram of findings 11 4               xii     Chapter 1: Introduction 1. 1 Dengue 1. 1 .1 Dengue – a disease of global significance Dengue has emerged ... DEN Figure 1- 2 Distribution of DENVs 1- 4 in (A) 19 70 and (B) 2 011 Compared to the 19 70s, the increased incidence and spread of all four dengue serotypes observed in 2 011...

Ngày tải lên: 09/09/2015, 08:20

... Summary of results In summary, we investigated if proteasome inhibition can inhibit other parts of the viral life cycle besides viral entry In Section 3. 1, we established a mosquito infection model ... exocytosis To elucidate the mechanisms involved, we show in Section 3. 3 that perturbation of the UPP leads to ER stress-induced UPR that represses translation of k...

Ngày tải lên: 22/09/2015, 15:18

... on eIF2α by the guanine nucleotide exchange factor eIF2B, thereby inhibiting recycling of the ternary complex that contains the initiator methionine Met-tRNAi (Dever, 20 02; Sonenberg & Hinnebusch, ... validate the function of UBE2A and DDB1 in the DENV life cycle, we tested if silencing of these two genes would also decouple infectious DENV -2 production from RNA repli...

Ngày tải lên: 22/09/2015, 15:18

... amino termini and one of several protein-protein interaction motifs carboxy ter- The ubiquitin-proteasome pathway in cell cycle control 157 minal to the F-box In addition, some members of the F-box ... lethality in a metazoan The ubiquitin-proteasome pathway in cell cycle control 169 References Agarwal R, Tang Z, Yu H, Cohen-Fix O (2003) Two distinct pathwa...

Ngày tải lên: 25/10/2013, 21:20

... These inflammatory cytokines are key factors for activity of the Ub-proteasome system [36] Thus, insulin may inhibit the activity of the Ub system by inhibiting inflammatory cytokines However, the ... sepsis currently exist In this study, we hypothesize that infusion of insulin would alleviate degradation of skeletal muscle protein by inhibiting the Ub-...

Ngày tải lên: 11/08/2014, 06:23

... pathway Interestingly, many of the genes in these pathways are regulated by the proteasome [18] Therefore, combined with our results, this may also help explain why targeting one aspect of a signaling ... Subsequently, TRAF6 interacts with UBC13/UEV1A, a heterodimer that catalyzes the synthesis of polyubiquitin chains assembled through linkage of the carboxyl terminus o...

Ngày tải lên: 13/08/2014, 16:21

... levels of BTG1 and BTG2 mRNAs increase in the early G1 phase of the cell cycle [4,5] and that BTG/Tob family proteins are involved in G1 arrest [7,11,13]; BTG/Tob family proteins accumulate at the ... degradation signal [40] Based on the results of our analyses of four members of the BTG/Tob family, we propose that the C-terminal region of this family...

Ngày tải lên: 31/03/2014, 21:21

... interact with Vpr [20] Bands a and b could not be identified Figure arrest the ubiquitin proteasome system in Vpr- induced G2 Role of Role of the ubiquitin proteasome system in Vpr- induced G2 arrest ... [9,10], HIV-1 Vif [11-13], and HIV-1 Vpu [14] In the present study, we examined in detail the potential role of the UPS in the ability of HIV-1 Vpr...

Ngày tải lên: 18/06/2014, 18:20

... interact with Vpr [20] Bands a and b could not be identified Figure arrest the ubiquitin proteasome system in Vpr- induced G2 Role of Role of the ubiquitin proteasome system in Vpr- induced G2 arrest ... [9,10], HIV-1 Vif [11-13], and HIV-1 Vpu [14] In the present study, we examined in detail the potential role of the UPS in the ability of HIV-1 Vpr...

Ngày tải lên: 20/06/2014, 01:20

... critical role in binding host cell receptors DII consist of the fusion loop that interacts with the endosomal membrane for fusion of the virus during entry into the cell (Rey et al, 1995) The hinge region ... expressing Fc RI and Fc RII (THP-1) and human myelogenous erythroleukemic cells (K562) expressing Fc RII, this thesis examined how different activating Fc Rs in...

Ngày tải lên: 09/09/2015, 08:11

... disruption of the STAT1 gene in mice revealed a role for STAT1 in the JAK-STAT signaling pathway [40] The JAK-STAT signaling pathway is involved in mediating biologic responses induced by many cytokines ... Post Infection Figure Replication of HSV-1 in macrophages Replication of HSV-1 in macrophages Analyses of virus replication, viral gB mRNA, and...

Ngày tải lên: 12/08/2014, 04:21

... disruption of the STAT1 gene in mice revealed a role for STAT1 in the JAK-STAT signaling pathway [40] The JAK-STAT signaling pathway is involved in mediating biologic responses induced by many cytokines ... Post Infection Figure Replication of HSV-1 in macrophages Replication of HSV-1 in macrophages Analyses of virus replication, viral gB mRNA, and...

Ngày tải lên: 12/08/2014, 04:21

... preserved by the treatment Another indication of the importance for indirect cell killing comes from studies of "naturally-infected" macaques i.e., sooty mangabeys infected with SIVsmm that maintain ... sparing the fraction of infected cells Such a process will tend to increase the proportion of infected cells and diminish the proportion of uninfected cells in a tissue h...

Ngày tải lên: 12/08/2014, 23:22

... around the involvement of lipids during an influenza virus infection There is still a substantial lack in the understanding of the role of lipids during virus infections, especially influenza virus ... 1.2.2 Host proteins and their involvement in influenza virus infection .8 1.2.3 Involvement of host metabolism in influenza virus infection...

Ngày tải lên: 12/10/2015, 17:36