Solubility studies, rational amino acid replacements and structural analyses of streptomyces jumonjinensis isopenicillin n synthase 1

... Cloning strategy for sjIPNS and nIPNS The gene sequences of sjIPNS and nIPNS were known (Shiffman et al., 19 88; Coque et al., 19 91) but that of sfIPNS was unknown and had to be determined Hence, ... annealing to and incorporating single Nde1 and BamH1 site at the respective 5’ and 3’ end of full-length sfIPNS gene was needed The principles behind choosing these sites for...

Ngày tải lên: 17/09/2015, 17:20

... isozyme sjIPNS scIPNS sfIPNS slIPNS nIPNS sjIPNS scIPNS sfIPNS slIPNS nIPNS sjIPNS scIPNS sfIPNS slIPNS nIPNS sjIPNS scIPNS sfIPNS slIPNS nIPNS sjIPNS scIPNS sfIPNS slIPNS nIPNS Total number of residues ... Summary of scIPNS mutants constructed Amino acid position in scIPNS Amino acid residue in scIPNS Amino acid residue in sjIPNS scIPNS single mutant constructed 120 Gly Glu G...

Ngày tải lên: 17/09/2015, 17:20

... subcloning into pGK and pET43.1a fusion vectors The creation of GST-sjIPNS and NusA-sjIPNS fusion constructs requires the cloning of sjIPNS gene downstream to the 3’ ends of the coding genes of ... respectively, hence the expected sizes of GST-sjIPNS and NusA-sjIPNS fusion proteins are ~63kDa and ~92kDa In recombinant vector pNUSA-SJ, sjIPNS is subcloned downstream of the...

Ngày tải lên: 17/09/2015, 17:20

... N-acylsulfonamide-linked dinucleoside inhibitors of RNase A [1], and has numerous interesting homologs [24] In humans, angiogenin (RNase 5) is an inducer of neovascularization, and plays an ... Journal 278 (2011) 541549 ê 2011 The Authors Journal compilation ê 2011 FEBS N Thiyagarajan et al A B C D N-acylsulfonamide-linked dinucleoside inhibitors of RNase A upon b...

Ngày tải lên: 14/02/2014, 22:20

... treatment with neuraminidase (as indicated by – and +, respectively) The sialylated tetrasaccharide-containing glycoforms are indicated by an asterisk All strains were grown on media containing ... that a terminal Sial-lNnT unit is linked to the Hep I residue of the LPS inner core in the sialylated glycoform The structure of the sialylated glycoforms of the O...

Ngày tải lên: 23/03/2014, 21:21

... monomeric antenna proteins (CP26, CP29 and LHCII monomers) (band 2), and tri4620 meric LHCII (band 3), monomeric (band 4) and dimeric (band 5) PSII cores Bands and contained supramolecular complexes of ... the biochemical analyses reported above A Electron cryomicroscopy and image analysis of the two-dimensional arrays B Fig Analysis of polypeptide composition of grana...

Ngày tải lên: 30/03/2014, 10:20

... domain of L2 to be conserved among the alpha-PVs irrespective of high or low risk group However, the structural interaction of L1 and L2 and formation of capsid is still not clear Minor capsid ... predict the secondary structure of L2 We also mapped the interaction domain of L2 within the monomer of L1 Our data shows that the amino terminus of L2 is i...

Ngày tải lên: 12/08/2014, 04:21

... Kumagaye KY, Nakajima K, Watanabe T, Kawai T, Kawakami Y, Niidome T, Sawada K, Nishizawa Y et al (1994) Omega-agatoxinTK containing D-serine at position 46, but not synthetic omega-[L-Ser46]agatoxin-TK, ... Inoue A, Kawakami Y, Nishizawa Y, Katayama K & Kuwada M (1995) Isolation and characterization of a peptide isomerase from funnel web spider venom J Biol Chem 270, 16719–16723 To...

Ngày tải lên: 18/02/2014, 17:20

... devoid of a given essential amino acid Our data suggest that the GCN2 pathway is directly involved in the regulation of amino acid and protein metabolism, as many of the genes involved in these processes ... their biological processes reveals that amino acid limitation regulates groups of genes that are involved in amino acid and pro...

Ngày tải lên: 07/03/2014, 03:20

... corresponding to the introns of the two genes The fragments were sequenced and enabled the complete reconstruction of the Psy1 and Psy2 genes with the annotation of introns and exons The GenBank ... based on the lack of information regarding the 5¢-region in the Psy2 DNA sequence and from the conflicting evidence available in the database records of t...

Ngày tải lên: 07/03/2014, 05:20

... the operation of the PPP is obtained from the f-values of His Ó FEBS 2004 Metabolic flux profiling of Pichia pastoris (Eur J Biochem 271) 2467 Table Relative abundances of intact C2 and C3 fragments ... generation and analysis of 2D [13C,1H] COSY NMR spectra for metabolic flux analysis purposes Metab Eng 3, 322–343 Walsh, K & Koshland, D.E (1984) Determination of...

Ngày tải lên: 23/03/2014, 12:20

... this hypothesis We find that V38E mutant is incapable of inducing bystander apoptosis in the presence of significant infection and replication in SupT1 cell line Interestingly bystander apoptosis ... Retrovirology 2007, 4:89 doi:10.1186/1743-422X-8-34 Cite this article as: Garg et al.: Single amino acid change in gp41 region of HIV-1 alters bystander apo...

Ngày tải lên: 11/08/2014, 21:21

... TOR REGULATORY PATHWAYS IN YEAST Two important nutrient sensing and regulatory pathways, the general amino acid control (GAAC) and the target of rapamycin (TOR) , participate in the control of yeast ... following amino acid starvation, including the mRNA for the general amino acid permease Gap1p, a protein critical to nitrogen regulation in yeas...

Ngày tải lên: 24/08/2014, 12:49

... (0.04) (0 .19 ) (0.09) 19 83 2493 14 86 18 85 10 30 16 30 13 45 10 38 19 60 805 514 (406) (13 74) (780) ( 710 ) ( 613 ) (809) (740) (542) (10 47) (752) (277) 19 64 15 61 1008 15 96 905 12 97 11 42 468 14 03 276 206 ... S1 Bar graph of R1, R2 and {1H} 15 N NOE values along the sequence of the TTF1 HD at 11 .7 T and 286 K 448 Fig S2 Plot of J(0.87xN)–J(0) correlati...

Ngày tải lên: 18/02/2014, 16:20

... b-amylase (Cys83, Cys96, Cys209 and Cys344) are homologous to those found in soybean b-amylase (Cys82, Cys97, Cys208 and Cys343) On the analogy of the soybean b-amylase, the active site of the ... for the b-amylase from C sepium using the X-ray coordinates of the soybean b-amylase (Fig 4) According to the Ramachandran plot of this model the f and c...

Ngày tải lên: 22/02/2014, 07:20