Molecular basis of agrobacterium mediated gene transfer into mammalian cells 4

... _ _ + + + + pH chvG 6 6) 7) G G 14 P3 A A C pL 40 07 pT 6( 6( 63 60 G G A A A 0-A 34 40 63 A A Fig 4. 4 The effects of ChvG on the expression of acid-inducible genes aopB and katA as determined ... bacterial cells were analyzed in the absence (-) [A6 340 , A6 340 -CG9, A6 340 (pJYH15) and A6 340 -AG6] or presence (+) [A6007-CG9, A6007(pJYH15), A6007-AG6, A6 340 -AG6(pLP36) and A6 34...

Ngày tải lên: 17/09/2015, 17:20

... functions in different ways 5. 2 Future prospective Agrobacterium was demonstrated to be able to deliver its DNA into mammalian cells Nonetheless, the precise mechanism of gene transfer is still unclear ... occurrence of horizontal gene transfer among prokaryotes is well established, the gene transfer between bacteria and eukaryotic cells is less well elucidated The...

Ngày tải lên: 17/09/2015, 17:20

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Ngày tải lên: 17/09/2015, 17:20

... Dissolved in dH2O, filter sterilized 24 24 24 X-Gal Dissolved in dimethyl sulfoxide 20 20 20 Proteinase K Dissolved in dH2O 20 50 50 RNase Dissolved in dH2O 10 20 20 51 Table 2. 4 Mammalian and ... study of the staining pattern of rhodamine phalloidin, mammalian cells were seeded on the glass cover-slip in 6-well tissue culture plates 2. 8.3 Agrobacterium- mediated ge...

Ngày tải lên: 17/09/2015, 17:20

... T-DNA transfer will be described in detail 1. 1 .1 Roles of Ti-plasmid encoded virulence genes 1. 1 .1. 1 VirA/VirG, member of highly conserved class of two-component regulatory system Sensing of signal ... some mammalian cells 1. 2 .1 T-DNA transfer from A tumefaciens into yeast T-DNA transfer from A tumefaciens into S cerevisiae is very similar to T-DNA transfer...

Ngày tải lên: 17/09/2015, 17:20

... applications of transforming growth factor- beta (TGF -beta) Growth Factors 19 93, 8 :1- 9 Assoian RK, Komoriya A, Meyers CA, Miller DM, Sporn MB: Transforming growth factor- beta in human platelets ... transforming growth factor- beta cDNA DNA 19 87, 6:239-244 Miller DA, Pelton RW, Derynck R, Moses HL: Transforming growth factor- beta A family of growth...

Ngày tải lên: 09/08/2014, 01:21

... neuronal cells J Biotechnol 2006, 122:393-411 doi: 10.1186/1423-0127-17-44 Cite this article as: Lin et al., Sonoporation-mediated gene transfer into adult rat dorsal root ganglion cells Journal ... gene transfer by sonoporation require further investigation Conclusions This study demonstrates that sonoporation enables delivery of plasmids into dorsal root gan...

Ngày tải lên: 10/08/2014, 05:21

... heterogenicity of vtg gene family and expressions of multiple vtg genes were investigated using a model fish, the zebrafish (Danio rerio) The study of vtg genes provides valuable information for further ... Red tilapia (Oreochromis mossambica) was used to explore the potential of using Vtgs as DNA carriers for development of a novel method for producing transgenic...

Ngày tải lên: 16/09/2015, 17:13

... Receptor- mediated gene transfer 24 hr, DNA~Vtg-pLys 144 24 hr, DNA~Vtg-pLys 144 24 hr, DNA 24 hr, DNA 24 hr, DNA 48 hr, DNA~Vtg-pLys 144 48 hr, DNA~Vtg-pLys 144 48 hr, DNA 48 hr, DNA 60 55 % of total ... radioactivity % of recovered of whole fish 50 45 40 35 30 25 20 15 10 24 48 ovary 24 48 liver 24 48 gut 24 gill 48 24 48 heart 24 48 spleen 24 48 kidney 24 4...

Ngày tải lên: 16/09/2015, 17:13

... Chapter Expression of vtgs 3. 3 .3 Tissue localization of vtg mRNAs 3. 3 .3. 1 Expression of vtgs in the liver of female fish and E2 treated male fish 3. 3 .3. 1.1 Morphological observations of H&E stained ... of vtgs sizes of the amplified products were the same as estimated from vtg cDNA sequences, i.e., 1 73 bp for vtg1, 190 bp for vtg2 and 130 bp for vtg3 (Fig 3-...

Ngày tải lên: 16/09/2015, 17:13

... full-length cDNA sequences of vtg1-7 A8** A17 A 22 A30 A67 A80 A87 A119 A 126 A139 A183 A186 A1 92 A193 A 220 A 227 A248 A250 A2 52 A253 A256 A257 A259 A269 A2 72 A290 A295 A296 A300 A306 A3 42 A349 A368 A371 ... (24 0) 44% (28 8) 43% (23 5) 47% (24 7) 40% ( 325 ) 49% (22 2) 39% (316) 48% (26 5) 40% (20 8) 47% (25 9) 43% (24 9) 46% ( 328 ) 52% (20 0) 43% (20 5) 43% (21 8) 46% (25 2) 43...

Ngày tải lên: 16/09/2015, 17:13

... Chapter General Introduction 1. 1 Oogenesis and vitellogenesis 1. 1 .1 Oogenesis The development of an egg is known as oogenesis, which can be divided into different phases including 1) proliferation of ... harness of this gene transfer method will rely on the improvements of its reproducibility and the foreign gene integration rate 18 Chapter General Introduction D Par...

Ngày tải lên: 16/09/2015, 17:13

... BIO- NANOPARTICLES AND BIO- MICROFIBERS FOR IMPROVED GENE TRANSFER INTO GLIOMA CELLS YANG JINGYE (B Eng.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY NUS GRADUATE SCHOOL FOR INTEGRATIVE ... procedures to form bio- nanoparticles and bio- microfibers The findings in this study should enrich the development of gene therapies for CNS diseases, p...

Ngày tải lên: 12/09/2015, 09:10

... DNA binding domain and VP16 activation domain Various combinations of the EcR ligand binding domain, VP16 activation domain and GAL4 DNA binding domain were constructed (Fig 1A) and tested in NIH ... probably in uence the behavior of fusion protein upon binding to ligand Apparently, having activation domain (VP16) and DNA binding domain (GAL4), in that order, on the NH2-terminal end...

Ngày tải lên: 16/03/2014, 12:20

... principal function of IL-10 appears to be anti-inflammatory, limiting and eventually terminating inflammatory responses by inhibiting synthesis of monocyte and macrophage derived pro-inflammatory cytokines ... injected intra-articularly into rabbit knees A naïve control group of rabbits was also included Lavages were performed using saline on days and after adenoviral delivery,...

Ngày tải lên: 09/08/2014, 08:22