molecular basis of aav diversity

... details Department of Pediatrics, SUNY Upstate Medical University, Syracuse, NY, USA 2School of Biomedical Sciences, University of Newcastle, Newcastle, NSW, 2300, Australia 3Laboratory of Allergic ... design, design of display items, and writing of first and all subsequent drafts of the manuscripts JBD conceived and designed the study, collated data and assembled first draft of the manuscript ... TNF 1.7 1.4 4.6 2.2 IFNga 1.6 1.9 18.4 26.0 Expression of proinflammatory mediators detected by PCR array analysis of RNA from lung tissue of infected mice vs RNA from lung tissue from agematched...

Ngày tải lên: 12/08/2014, 02:20

... drives expression of a particular gene in the tissue/s of interest The objective of the current study was to identify which one of a group of promoters confers the greatest degree of cardiac specific ... the tissue of interest Several groups have demonstrated the unique ability of the AAV9 capsid to yield extraordinarily high levels of transgene expression in the heart [8-11] Each of these groups ... inverted terminal repeats of AAV2 and packaged into the AAV9 capsid to yield rAAV2/9-promoter-LacZ The constructs were tested by transfection and subsequently infection of both differentiated and...

Ngày tải lên: 14/08/2014, 19:22

... instances of micro-hemorrhage, T or B cells were observed in the CNS of any of the wildtype or APP-tg mice regardless of the immunization regime, providing further evidence for the lack of a deleterious ... parenchyma of the brain in two of the cases, suggesting a T cell mediated immune response to the vaccination In the other report, there was little evidence of overt inflammation at the time of death ... Neurologic Diseases (Boston, MA) Aβ1–15 peptide is a single copy of the first 15 amino acids of Aβ, whereas dAβ1–15 consists of 16 copies of the same peptide on a lysine backbone The peptides were...

Ngày tải lên: 19/06/2014, 22:20

... and (D) of untreated APP/IL-1 R1+/-; APP/IL-1 R1-/- and (B) 15-monthold APP/IL-1 R1-/-; (C) of Thioflavin-S-stained Aβ Tg2576 mice (IL-1 R1+/+) tained with Representative pictures of Thioflavin-S-stained ... C57BL/6.SJL) Groups of mice at various ages (6 months, months and 15 months of age) were killed and the levels of both SDSsoluble (SDS) and SDS-insoluble FA-extractable fractions of Aβ40 and Aβ42 ... For analysis of plaque burdens in the passive immunization experiments, immunostained plaques were quantified (proportional area of plaque burden) in the neocortex of the same plane of section...

Ngày tải lên: 19/06/2014, 22:20

... as the source of total RNA for RT-PCR reactions For the initial RT-PCR amplification of hprt mRNA, µl of the cell lysate was used in a final volume of 20 µl RT-PCR containing µl of 10×VM buffer ... expected by addition of their individual responses The study of these interactions can lead to a better understanding of the toxic mechanism of the chemicals involved A number of terms have been ... percentage versus the number of total clones Table DNA sequence analysis of hprt mutant in splenic cells of B6C3F1 female mice in 52-wk study Type of mutation Number of mutants Control Ozone NNK...

Ngày tải lên: 07/08/2014, 18:20

... standard error of the mean of the combined data of triplicate determination of triplicate experiments performed on a pool of RA SF cultures from five RA patients One-way analysis of variance with ... inhibitor of metalloproteinase (TIMP)-1, (f) TIMP-2 Results are the mean ± standard error of the mean of the combined data of triplicate determination of triplicate experiments done on a pool of RA ... production of diverse mediators of inflammatory conditions, is also implicated in the pathophysiology of RA [4] Among the vast family of PLA2 enzymes, which includes three cellular (cPLA2) isoforms...

Ngày tải lên: 09/08/2014, 14:22

... the differential pathogenesis of a point mutant of HIV While the use of a laboratory adapted X4 (Lai) isolate is also a limitation of our study the major emphasis of our study is on using the ... CD4 cell decline in the presence of similar levels of virus replication Hu-HSC mice were infected with 50,000 TCID50 of viruses and bled every weeks for a total of weeks and then sacrificed (A) ... loss of CD4 cells mediated by WT and V38E virus in the presence of similar levels of viremia is a strong indicator of the role of gp41 in CD4 loss Our study also addressed the question whether...

Ngày tải lên: 11/08/2014, 21:21

... decreased activation of thrombomodulin and protein C as well as reduction of anti-fibrinolysis and enhancement of plasminogen activator inhibitor (PAI)-1 expression [2] The production of procoagulant ... with the German legislation on protection of animals and the National Institutes of Health 'Guide for the Care and Use of Laboratory Animals' (Institute of Laboratory Animal Resources, National ... with a core body temperature of 37°C, 24 h endotoxemia caused a drastic increase of sPAI-Ag when compared to 37°C saline controls (Figure 2) Of interest, hypothermia of 34°C and 31°C in endotoxemic...

Ngày tải lên: 13/08/2014, 03:20

... importance of HIV DNA in the pathogenesis of HAD Other limitations of the current study include the assumption that the CD14- subsets were composed mainly of CD4 lymphocytes The calculations of HIV ... reservoir of virus in the majority of subjects who had been on HAART for longer than years [24] Calcaterra et al found higher levels of HIV DNA in monocytes than in CD4+ lymphocytes in a subset of non-viremic ... (P S.) established a diagnosis of HAD using standard -of- care approaches for Thailand In general, the evaluation included participant and proxy informant reports of symptoms and function, the HIV...

Ngày tải lên: 31/10/2012, 15:34

... LÝ THUYẾT CHIẾN LƯỢC KINH DOANH 2.1 Lý thuyết học giả: Theo Fred R David, tác giả sách Concepts of Strategic Management, “chiến lược phương tiện đạt tới mục tiêu dài hạn” Khác với quan niệm trên,...

Ngày tải lên: 25/01/2013, 09:29

... WARM UP Game: JUMBLED WORDS CCLOY  CYCLO RIEDV  DRIVE TALLS DOFO  FOOD STALL NSSEGERPA  PASSENGER PRE-LISTENING WHO IS HE? - He gets up very early - He has ... PRE-LISTENING Listen and repeat district:/'distrikt/ park:/pɑ:k/ routine:/ru:'ti:n/ pedal:/'pedl/ office:/'ɔfis/ food stall:/fu:d stɔ:l/ purchase:/'pə:t∫əs/ drop:/drɔp/ passengers:/'pæsindʒə/ ride:/raid/...

Ngày tải lên: 21/06/2013, 01:26

... 4-75 0.7-5 Molecular Weight (g) 2.016 170 Density of NTP gas (g/cm ) 0.0838 0.86 Mass Ratio (kg of air/kg of fuel) 34.4 15.2 Flame Velocity (cm/s) 270 30 Specific gravity 0.091 0.83 Heat of Combustion ... and ± 0.1 sec respectively For a Speed (N) of 1500 rpm, Voltage (V) of 230 volts, Current (I) of 14 A, Fuel volume (fx) of 10 cc and brake power (BP) of 3.7 kW the uncertainty in brake power calculation ... Average uncertainties of some measured and calculated parameters S.No Parameters Speed Temperature Mass flow ratye of air Mass flow rate of diesel Mass flow rate of hydrogen Oxides of nitrogen Hydrocarbon...

Ngày tải lên: 05/09/2013, 15:28

... climax, and the conclusion of the story Unit 1: A day in the life of Period 4: Writing Task 2.Task 2: Format of a narrative Climax Events Conclusion Unit 1: A day in the life of Period 4: Writing ... form of these verbs: be tell begin think was, were told began thought have get live help had got lived helped Unit 1: A day in the life of Period 4: Writing Vocabulary land (v): h¹ c¸nh take off ... A day in the life of Period 4: Writing 1.Task 1: Key: The connectors + on that day + at first + then + just + a few minutes late + one hour later Unit 1: A day in the life of Period 4: Writing...

Ngày tải lên: 18/09/2013, 05:10

Ngày tải lên: 19/09/2013, 14:10

... solution to almost all problems of cross−platform compatibility Now, we're big fans of Java − we've used it in the past, and will so again in the future − but this series of tutorials isn't about Java ... of tutorials isn't about Java It's actually about an offshoot of Java, the innocuously−named Java Server Pages, or JSP, which attempts to offer Web developers a compelling alternative to traditional ... interactivity to their Web sites, a bunch of programming languages were born in order to meet increasingly complex requirements The best−known of these is, of course, Perl, although PHP and ASP are...

Ngày tải lên: 19/10/2013, 02:15

Ngày tải lên: 28/10/2013, 17:15

... activities: T: Lan, what time you often get up? Lan:: I often get up at 6.oo T: Phong, what you often in the afternoon? Phong: I go to school T: Hoa, what time you often go to the bed? Hoa: I always ... video about linh’ daily routine in minute -ask ss to remember activities and time’s activities of Linh -2 ms for teams go to the board and write answers -team which more correct answer is winner...

Ngày tải lên: 02/12/2013, 19:11